Bacterial biofilms: a possible mechanism for chronic infection in patients with lumbar disc herniation - a prospective proof-of-concept study using fluorescence in situ hybridization.

A relationship has been suggested between lumbar disc herniation (LDH) and chronic bacterial infection frequently involving Propionibacterium acnes, which is known to cause chronic infection through the formation of biofilm aggregates. The objective of the study was to assess whether a disc infection involving biofilm formation is present in patients with LDH. A total of 51 LDH patients and 14 controls were included. Bacterial DNA was detected by real-time polymerase chain reaction (PCR) in 16/51 samples in the LDH group and 7/14 controls (p = 0.215). Sequencing identified bacteria in 9/16 and 6/7 PCR positive samples in the LDH and control groups, respectively. All samples were stained using fluorescence in situ hybridization (FISH) and examined by confocal laser scanning microscopy. Microscopy demonstrated tissue-embedded bacterial aggregates with host inflammatory cells in 7/51 LDH patients and no controls. The presence of both bacterial aggregates and inflammatory cells suggests a chronic infection in a subset of LDH patients. The finding of bacterial 16S rDNA in both LDH and control disc tissue highlights the importance of microscopic observation to discriminate infection vs contamination. Our findings may have therapeutic implications, as the treatment of biofilm infections is different and more challenging than traditional infections.

Traumatic atlantoaxial rotatory dislocation and displaced ossiculum terminale epiphysiolysis treated with a halo device: a case report.

INTRODUCTION: The horizontal synchondroses of the infant axis are biomechanical weak regions in trauma. CASE: We report the case of a 6-year-old girl who presented with delayed atlantoaxial dislocation and displaced ossiculum terminale epiphysiolysis after a fall from a tree. TREATMENT: The patient was treated with halo traction for 3 days after which a CT scan showed normal position of the C1/C2 joint, and an acceptable position of the ossiculum terminale whereafter a halo brace was applied. Because of delayed union on CT scans, the treatment was prolonged to a total of 21 weeks. RESULT: At final follow-up 26 months after halo brace removal, the patient demonstrated normal range of movement of the neck on clinical examination and radiographs of the cervical spine including lateral flexion/extension radiographs showed no sign of instability of the atlantoaxial joint. DISCUSSION: Conservative treatment with a halo device versus surgical treatment is discussed.

Inter- and Intra-rater Agreement in Assessment of Adult Spinal Deformity Using the Scoliosis Research Society-Schwab Classification.

STUDY DESIGN: Cross-sectional assessment of inter- and intra-rater agreement. OBJECTIVES: To assess inter- and intra-rater agreement between spine surgeons with different levels of experience in a large consecutive series of adult patients referred to a tertiary institution for evaluation of a spinal deformity using the Scoliosis Research Society (SRS)-Schwab classification. BACKGROUND: The development of the SRS-Schwab classification of adult spinal deformity is based on current knowledge about relevant radiographic variables related to health-related quality of life. Clinical implementation of the classification requires satisfactory reliability. Two recent reports on agreement were based on a small selected case sample using pre-marked radiographs and a large cohort of only surgical patients, including congenital deformity. METHODS: Scoliosis Research Society-Schwab classification of 67 consecutive adult patients referred for surgical evaluation of a spinal deformity was done in a blinded fashion without pre-marking by 2 senior spine surgeons, 1 attending, and 1 spine fellow. After 2 weeks, the classification was repeated on recoded radiographs. Inter- and intra-rater agreement was analyzed using Fleiss kappa statistics. Rater bias was assessed using Bhapkar test for marginal homogeneity. RESULTS: According to Landis and Koch, the observed agreements were considered substantial to almost perfect for curve type and sagittal modifiers and moderate for entire grade, with no consistent differences between surgeons with different levels of experience. CONCLUSIONS: The results for each individual radiographic parameter correspond to previous findings and support use of the SRS-Schwab classification in adult spinal deformity. The results for entire grade were considered only moderate. The authors recommend that it is not used as an individual parameter.