Diagnostic and prognostic significance of miR-451a in patients with atherosclerosis.

OBJECTIVES: Atherosclerosis (AS) is a chronic inflammatory vascular disease. This study aimed to detect the expression level of miR-451a and investigate the diagnostic and prognostic values of miR-451a for AS patients. METHODS: The relative expression of miR-451a was assessed by qRT-PCR. Comparison of groups was analyzed with the t-test and chi-squared test. Pearson analysis was used to validate the correlation of miR-451 with CRP and CIMT. The receiver operating characteristic (ROC) curves, K-M analysis, and Cox regression analysis were conducted to explore the roles of miR-451a in diagnosing AS patients and predicting outcomes of AS patients. RESULTS: The expression of miR-451a was significantly decreased in the serum of AS patients. The results of Pearson analysis showed the expression of miR-451a was negatively correlated with CRP and CIMT. The data of ROC proposed miR-451a could differentiate AS patients from healthy individuals with high sensitivity and specificity. K-M analysis and Cox regression showed miR-451a might be an independent biomarker of suffering cardiovascular endpoint diseases in AS patients. The expression of miR-451a was obviously inhibited in AS patients with cardiovascular endpoint events. CONCLUSION: Deregulation of miR-451a might be associated with the development of AS. MiR-451a might be used as a promising diagnostic and prognostic biomarker for clinical treatment of AS patients.

Association of miR-192-5p with Atherosclerosis and its Effect on Proliferation and Migration of Vascular Smooth Muscle Cells.

To evaluate the diagnostic significance of serum miR-192-5p for atherosclerosis (AS) and explore the effect of miR-192-5p on cell proliferation and migration of vascular smooth muscle cells (VSMCs). The expression level of serum miR-192-5p was measured by qRT-PCR. Correlations of miR-192-5p with CRP and CIMT were evaluated by Pearson correlation coefficient. The diagnostic significance of miR-192-5p was assessed using an ROC curve. CCK-8 assay and Transwell assay were used to analyze the effect of miR-192-5p on cell proliferation and migration. Luciferase reporter gene assay was used to evaluate the effect of miR-192-5p with ATG7. The expression level of serum miR-192-5p in AS patients was significantly high compared with healthy controls. miR-192-5p was positively correlated with CRP and CIMT, and it has diagnostic value for AS. In vitro cell experiments confirmed that overexpression of miR-192-5p could promote cell proliferation and migration of VSMCs. miR-192-5p directly targets ATG7 in VSMCs. Down-regulation of miR-192-5p level increased ATG7 expression and inhibited cell proliferation and migration. miR-192-5p may be a new biomarker for the diagnosis of AS and may provide new idea for the treatment of AS.