[Research on nondestructive measurement of soluble tannin content of astringent persimmon using visible and near infrared diffuse reflection spectroscopy].

The objectives of the present study were to establish the relationships of the visible and near infrared diffuse reflection (Vis/NIR) spectroscopy and the soluble tannin content of internal quality index of astringent persimmon, and to evaluate the value of Vis/NIR spectroscopy in measuring the soluble tannin content of internal quality index of astringent persimmon. In the spectral region between 570 and 1 848 nm, calibration results for the soluble tannin content of astringent persimmon were compared with different regression techniques, different derivative treatments and different scatter and standard treatments. The results showed that the modified partial least squares(MPLS) model, with respect to the first derivative D1 log(1/R) and detrend only, provided better prediction performance for the soluble tannin content of astringent persimmon fruit, with the correlation coefficient of cross validation of calibration (R(cv)) and correlation coefficient of prediction (R(p2)), the root mean square error of cross validation of calibration (RMSECV) and the root mean square error of prediction (RMSEP) of 0.722 7, 0.678 5, 0.148, and 0.176 3 respectively. The preliminary research on the built models indicated that nondestructive measurement of the soluble tannin content of astringent persimmon using Vis/NIR spectroscopy technique was feasible, but the precision of the models could be improved further.

Sustained release of insulin-like growth factor-1 from poly(lactide-co-glycolide) microspheres improves osseointegration of dental implants in type 2 diabetic rats.

Dental implantation is an effective and predictable treatment modality for replacing missing teeth and repairing maxillofacial defects. However, implants in patients with type 2 diabetes mellitus are likely to have a high failure rate and poor initial osseointegration. In the current study, we established an effective drug delivery system designed to improve osseointegration of dental implants in an animal model of type 2 diabetes. Twenty type 2 diabetic rats were divided into two groups: a group receiving recombinant rat Insulin-like Growth Factor 1 (rrIGF-1) Microsphere Therapy (MST) (10 rats) and a control group (10 rats). The rrIGF-1 was encapsulated into poly(lactide-co-glycolide) (PLGA) microspheres to produce a sustained-release effect around titanium (Ti) dental implants in the rrIGF-1 MST group. Scanning electron microscopy, confocal laser scanning microscopy, and cumulative-release studies were conducted to verify the release effect of the microspheres as well as rrIGF-1 bioactivity. Five rats from each group were sacrificed at weeks 4 and 8 post surgery, and a histological analysis was performed on the rats from both groups. Compared to the control group, rats that received rrIGF-1 by PLGA microsphere treatment were observed to have a higher bone-implant contact percentage around the Ti implants at week 4 or week 8 post surgery (P<0.05). This result clearly indicates that sustained release of rrIGF-1 through encapsulation by PLGA microspheres positively affects osseointegration of dental implants in type 2 diabetic rats.

Type 2 diabetes mellitus impairs bone healing of dental implants in GK rats.

Type 2 diabetes is an increasingly prevalent disease with oral health manifestations. In this study, titanium implants were placed in the femora of 10 type 2 diabetic and 10 age-matched normal rats. We compared the results of bone histomorphometry around the dental implants at 4 and 8 weeks postsurgery.