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1.
Mar Drugs ; 22(3)2024 Feb 29.
Article in English | MEDLINE | ID: mdl-38535459

ABSTRACT

Seven new phenol derivatives, subversins A-E (1-5), subversic acid A (6) and epi-wortmannine G (7); one new natural product, 4-hydroxy-7-methoxyphthalide (8); and five known compounds (9-13) were isolated from the fungus Aspergillus subversicolor CYH-17 collected from the Haima cold seep. The structures and absolute configurations of these compounds were determined via NMR, MS, optical rotation, electronic circular dichroism (ECD) calculation, X-ray diffraction analysis and comparison with the literature. Compounds 2 and 5 were two pairs of enantiomers. All compounds were tested for their α-glucosidase and acetylcholinesterase (AChE) inhibitory activity, antioxidant activity and antibacterial activity, but no obvious activity was observed among these studied compounds.


Subject(s)
Acetylcholinesterase , Aspergillus , Phenol , Phenols , Fungi
2.
Nat Prod Res ; : 1-6, 2023 Dec 15.
Article in English | MEDLINE | ID: mdl-38099373

ABSTRACT

One novel diketopiperazine derivative 8R-methoxy-9R-hydroxyl-fumitremorgin C (1), together with twelve known compounds, was separated from the fungus Aspergillus fumigatus CYH-5 collected from Haima cold seep. The structures of the compounds were identified by NMR, MS, optical rotation, hydrolysis reaction and comparing with literatures. Among them, compounds 10 and 11 exhibited inhibitory effect against bacteria. Compound 11 showed inhibitory activity on α-glucosidase and compound 8 displayed acetylcholinesterase (AchE) inhibitory activity.

3.
Nat Prod Res ; 37(3): 389-396, 2023 Feb.
Article in English | MEDLINE | ID: mdl-34498972

ABSTRACT

A new indole diketopiperazine alkaloid, named penilline D (1), together with five known indole alkaloid analogues (2-5, 11), two meroterpenoids (6 and 12), and four butenolide derivatives (7-10), were isolated from the Antarctic fungus Penicillium sp. SCSIO 05705. Extensive spectroscopic analysis and electronic circular dichroism (ECD) calculation were used to elucidate the structure of penilline D (1), including its absolute configuration. All isolated compounds (1-12) were evaluated for their cytotoxic, antibacterial and enzyme inhibitory activities against acetylcholinesterase (AChE) and pancreatic lipase (PL). Among them, compound 5 exhibited moderate in vitro cytotoxic activity against the 143B cell line with IC50 value of 12.64 ± 0.78 µM. Compound 6 showed strong inhibitory activity against AChE with IC50 value of 0.36 nM (IC50 18.7 nM for Tacrine), while compounds 6 and 11 showed weak PL enzyme inhibitory activity. Furthermore, an in silico molecular docking study was also performed between 6 and AChE.


Subject(s)
Antineoplastic Agents , Penicillium , Polyketides , Acetylcholinesterase , Circular Dichroism , Diketopiperazines , Indole Alkaloids , Molecular Docking Simulation , Molecular Structure , Penicillium/chemistry , Polyketides/chemistry
4.
Nat Prod Res ; 37(20): 3512-3518, 2023.
Article in English | MEDLINE | ID: mdl-35722895

ABSTRACT

A new furanone analog, (E)-2-(8,9-dihydroxy-6,8-dimethyldec-4-en-2-yl)-met-hylfuran-3(2H)-one (1), together with six known compounds, including two diterpenoids (2 and 3), one butyrolactone (4) and three isocoumarins (5-7), were isolated from a deep-sea fungus, Purpureocillium sp. SCSIO 06693. Among them, compound 1 existed as two tautomeric forms (1a and 1b) differing in configuration of the furan ring. The chemical structures were elucidated by the basis of spectroscopic evidences, including HRESIMS, NMR and optical rotation. Isolated compounds were evaluated for their cytotoxic, antiviral, antibacterial, antioxidant, acetyl cholinesterase (AChE) and pancreatic lipase (PL) enzyme inhibitory activities. Biological evaluation results revealed that compound 4 showed modest antioxidant activity against DPPH with IC50 value of 72.03 µM. In addition, compounds 1-4 exhibited PL enzyme inhibitory activities.

5.
Mar Drugs ; 20(1)2022 Jan 14.
Article in English | MEDLINE | ID: mdl-35049926

ABSTRACT

Six new α-pyrone meroterpenoid chevalones H-M (1-6), together with six known compounds (7-12), were isolated from the gorgonian coral-derived fungus Aspergillus hiratsukae SCSIO 7S2001 collected from Mischief Reef in the South China Sea. Their structures, including absolute configurations, were elucidated on the basis of spectroscopic analysis and X-ray diffraction data. Compounds 1-5 and 7 showed different degrees of antibacterial activity with MIC values of 6.25-100 µg/mL. Compound 8 exhibited potent cytotoxicity against SF-268, MCF-7, and A549 cell lines with IC50 values of 12.75, 9.29, and 20.11 µM, respectively.


Subject(s)
Anthozoa , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Aspergillus , Pyrones/pharmacology , Animals , Anti-Bacterial Agents/chemistry , Antineoplastic Agents/chemistry , Aquatic Organisms , Cell Line, Tumor , China , Humans , Inhibitory Concentration 50 , Microbial Sensitivity Tests , Pyrones/chemistry
6.
Mar Drugs ; 19(10)2021 Sep 26.
Article in English | MEDLINE | ID: mdl-34677441

ABSTRACT

To enlarge the chemical diversity of Eurotium sp. SCSIO F452, a talented marine-derived fungus, we further investigated its chemical constituents from a large-scale fermentation with modified culture. Four pairs of new salicylaldehyde derivative enantiomers, euroticins F-I (1-4), as well as a known one eurotirumin (5) were isolated and characterized. Compound 1 features an unprecedented constructed 6/6/6/5 tetracyclic structures, while 2 and 3 represent two new types of 6/6/5 scaffolds. Their structures were established by comprehensive spectroscopic analyses, X-ray diffraction, 13C NMR, and electronic circular dichroism calculations. Selected compounds showed significant inhibitory activity against α-glucosidase and moderate cytotoxic activities against SF-268, MCF-7, HepG2, and A549 cell lines.


Subject(s)
Aldehydes/pharmacology , Antineoplastic Agents/pharmacology , Antioxidants/pharmacology , Eurotium , Aldehydes/chemistry , Animals , Antineoplastic Agents/chemistry , Antioxidants/chemistry , Aquatic Organisms , Cell Line, Tumor/drug effects , Humans , Molecular Structure , Stereoisomerism
7.
Fitoterapia ; 150: 104839, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33513431

ABSTRACT

Three new tricyclic cyclopiazonic acid (CPA) related alkaloids asperorydines N-P (1-3), together with six known compounds (4-9) were isolated and characterized from the fungus Aspergillus flavus SCSIO F025 derived from the deep-sea sediments of South China Sea. The structures including absolute configurations of 1-3 were deduced from spectroscopic data, X-ray diffraction analysis, and electronic circular dichroism (ECD). All compounds were evaluated for the antioxidative activities against DPPH, cytotoxic activities against four tumor cell lines (SF-268, HepG-2, MCF-7, and A549), and antimicrobial activities. Compound 9 showed significant radical scavenging activities against DPPH with an IC50 value of 62.23 µM and broad-spectrum cytotoxicities against four tumor cell lines with IC50 values ranging from 24.38 to 48.28 µM. Furthermore, compounds 4-9 exhibited weak antimicrobial activities against E scherichia coli, and compound 9 also showed antibacterial activity against Bacillus thuringiensis, Micrococcus lutea, Staphylococcus aureus, Bacillus subtilis, Methicillin resistant Staphylococcus aureus.


Subject(s)
Alkaloids/pharmacology , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/pharmacology , Aspergillus flavus/chemistry , Indoles/pharmacology , Alkaloids/isolation & purification , Anti-Bacterial Agents/isolation & purification , Antineoplastic Agents/isolation & purification , Aquatic Organisms/chemistry , Bacillus/drug effects , Cell Line, Tumor , China , Escherichia coli/drug effects , Geologic Sediments/microbiology , Humans , Indoles/isolation & purification , Methicillin-Resistant Staphylococcus aureus/drug effects , Microbial Sensitivity Tests , Micrococcus/drug effects , Molecular Structure , Seawater/microbiology
8.
Nat Prod Res ; 34(14): 1984-1991, 2020 Jul.
Article in English | MEDLINE | ID: mdl-30721083

ABSTRACT

A new butenolide derivative (±)-asperteretal F (1) and related congener (2) recently reported containing an unusual 2-benzyl-3-phenyl substituted lactone core, together with five known compounds (3-7) were isolated and characterized from the fungus Aspergillus terreus. SCSIO FZQ028 derived from a deep-sea sediment of South China Sea. Their chemical structures were established on the basis of 1D- and 2D-NMR spectroscopic data, and HR-ESI-MS analysis. Additionally, all the compounds were evaluated for the antioxidative activities against DPPH, cytotoxic activities against two tumor cell lines (SF-268 and HepG-2), and antimicrobial activities. Compounds 2-4, and 7 showed significant activities against DPPH with IC50 ranging from 5.89 to 10.07 µg/mL. Compounds 2 and 4 showed moderate antimicrobial activities against all four tested bacteria.[Figure: see text].


Subject(s)
4-Butyrolactone/analogs & derivatives , Anti-Infective Agents/isolation & purification , Antineoplastic Agents/isolation & purification , Antioxidants/isolation & purification , Aspergillus/chemistry , 4-Butyrolactone/chemistry , 4-Butyrolactone/isolation & purification , 4-Butyrolactone/pharmacology , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Antioxidants/chemistry , Antioxidants/pharmacology , Bacteria/drug effects , Cell Line, Tumor , China , Fungi , Humans , Magnetic Resonance Spectroscopy , Mass Spectrometry , Molecular Structure
9.
Int J Syst Evol Microbiol ; 68(11): 3487-3493, 2018 Nov.
Article in English | MEDLINE | ID: mdl-30300120

ABSTRACT

A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198T, was isolated from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-positive, rod-shaped and salmon pink in colour. Good growth occurred on marine agar with 1-5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight. Sensitive to short ultraviolet radiation. Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198T had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868T, and loosely related (<94.2 %) to all other species in the genus Rubrobacter. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter. The total cellular fatty acid profile was dominated by C16 : 0 12-methyl. MK-8 was the main menaquinone. The peptidoglycan type was A3α (l-Lys-l-Ala). The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids. Based on these polyphasic data, a new species, Rubrobacterindicoceani sp. nov., is proposed, with the type strain SCSIO 08198T (=DSM 105148T=CGMCC 1.16398T).


Subject(s)
Actinobacteria/classification , Geologic Sediments/microbiology , Phylogeny , Actinobacteria/genetics , Actinobacteria/isolation & purification , Bacterial Typing Techniques , Base Composition , Cell Wall/chemistry , DNA, Bacterial/genetics , Fatty Acids/chemistry , Indian Ocean , Peptidoglycan/chemistry , Phospholipids/chemistry , Pigmentation , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Ultraviolet Rays , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistry
10.
Mar Drugs ; 16(4)2018 Apr 21.
Article in English | MEDLINE | ID: mdl-29690501

ABSTRACT

Three new prenylated indole 2,5-diketopiperazine alkaloids (1⁻3) with nine known ones (5⁻13), one new indole alkaloid (4), and one new bis-benzyl pyrimidine derivative (14) were isolated and characterized from the marine-derived fungus Eurotium sp. SCSIO F452. 1 and 2, occurring as a pair of diastereomers, both presented a hexahydropyrrolo[2,3-b]indole skeleton. Their chemical structures, including absolute configurations, were elucidated by 1D and 2D NMR, HRESIMS, quantum chemical calculations of electronic circular dichroism, and single crystal X-ray diffraction experiments. Most isolated compounds were screened for antioxidative potency. Compounds 3, 5, 6, 7, 9, 10, and 12 showed significant radical scavenging activities against DPPH with IC50 values of 13, 19, 4, 3, 24, 13, and 18 µM, respectively. Five new compounds were evaluated for cytotoxic activities.


Subject(s)
Alkaloids/chemistry , Aquatic Organisms/chemistry , Eurotium/chemistry , Fungi/chemistry , Alkaloids/pharmacology , Antioxidants/chemistry , Cell Line, Tumor , Circular Dichroism/methods , Crystallography, X-Ray/methods , Cytotoxins/chemistry , Cytotoxins/pharmacology , Diketopiperazines/chemistry , Diketopiperazines/pharmacology , Hep G2 Cells , Humans , Magnetic Resonance Spectroscopy/methods
11.
Fitoterapia ; 125: 49-54, 2018 Mar.
Article in English | MEDLINE | ID: mdl-29269234

ABSTRACT

Seven new compounds, including four new chlorinated diphenyl ethers, namely chrysines A-D (1-4), one new dichlorinated xanthone, chrysoxanthone (5), dichloroorcinol (6), and one new benzeneacetic acid derivative, 3-isopentyl-4-hydroxy phenylacetic acid methyl ester (7), along with fourteen known compounds (8-21), were isolated from a deep-sea-derived fungus Penicillium chrysogenum SCSIO 41001. Their structures were determined by extensive spectroscopic methods and X-ray single-crystal diffraction analysis. All of the isolated compounds (1-21) were evaluated for their α-glucosidase inhibitory activity using PNPG method. Among them, nine compounds (2, 3, 5, 6, 8, 9, 13, 17, and 18) exhibited inhibitory activity against α-glucosidase with IC50 values of 0.35, 0.20, 0.04, 0.16, 0.15, 0.09, 0.14, 0.14, and 0.12mM, respectively (IC50 0.28mM for the positive control acarbose).


Subject(s)
Glycoside Hydrolase Inhibitors/isolation & purification , Halogenated Diphenyl Ethers/isolation & purification , Penicillium chrysogenum/chemistry , Xanthones/isolation & purification , Molecular Structure , Seawater/microbiology , alpha-Glucosidases
12.
Antonie Van Leeuwenhoek ; 106(6): 1089-95, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25249446

ABSTRACT

A Gram-positive, rod-shaped bacterium, designated strain SCSIO N0306(T), was isolated from an abyssal sediment sample collected from the Indian Ocean. The isolate was found to grow optimally at 0-2 % (w/v) NaCl, pH 7.0 and 30 °C. Comparative analysis of the 16S rRNA gene sequence showed that the isolate SCSIO N0306(T) belongs phylogenetically to the genus Paenibacillus, and to be most closely related to P. algorifonticola XJ259(T) (with 95.47 % sequence similarity), sharing less than 95.0 % sequence similarity with all other taxa of this genus. Chemotaxonomic analysis revealed MK-7 as the major isoprenoid quinone, the DNA G+C content was determined to be 45.5 mol%, and anteiso-C15:0, C16:0, and iso-C15:0 were identified as the major fatty acids. On the basis of this polyphasic taxonomic data, isolate SCSIO N0306(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus abyssi sp. nov. is proposed. The type strain is SCSIO N0306(T) (= DSM 26238(T) = CGMCC 1.12987(T)).


Subject(s)
Geologic Sediments/microbiology , Paenibacillus/classification , Paenibacillus/isolation & purification , Bacterial Typing Techniques , Base Composition , Cluster Analysis , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Hydrogen-Ion Concentration , Indian Ocean , Microscopy, Electron, Transmission , Molecular Sequence Data , Paenibacillus/genetics , Paenibacillus/physiology , Phylogeny , Quinones/analysis , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sodium Chloride/metabolism , Temperature
13.
Int J Syst Evol Microbiol ; 64(Pt 3): 875-881, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24271213

ABSTRACT

A nitrogen-fixing bacterium, designated strain SCSIO N0430(T), was isolated from a mangrove sediment sample. Analysis of the sequence of the nifH gene responsible for nitrogen fixation in this strain indicated a close relationship to an uncultured bacterium ZNZ-D11 (GenBank accession no. JF896696). 16S rRNA gene sequence analysis revealed that this isolate had less than 93 % similarity to its closest relative, Sunxiuqinia elliptica DQHS4(T). A phylogenetic tree reconstructed based on 16S rRNA gene sequences revealed that strain SCSIO N0430(T) was a member of the phylum Bacteroidetes. Chemotaxonomic and physiological characteristics, including phospholipids and major fatty acids, readily distinguished the isolate from established members of the phylum Bacteroidetes. It is concluded that strain SCSIO N0430(T) represents a novel genus and species, for which the name Mangrovibacterium diazotrophicum gen. nov., sp. nov. is proposed, with the type strain of the species SCSIO N0430(T) ( = KCTC 32129(T) = DSM 27148(T) = JCM 19152(T)). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the three genera Sunxiuqinia, Prolixibacter and Mangrovibacterium are proposed to make up a novel family, Prolixibacteraceae fam. nov., in the order Bacteroidales.


Subject(s)
Bacteroidetes/classification , Geologic Sediments/microbiology , Nitrogen Fixation , Phylogeny , Bacteroidetes/genetics , Bacteroidetes/isolation & purification , Base Composition , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Genes, Bacterial , Molecular Sequence Data , Oxidoreductases/genetics , Phospholipids/chemistry , RNA, Ribosomal, 16S/genetics , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistry , Wetlands
14.
Antonie Van Leeuwenhoek ; 104(5): 829-36, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23934481

ABSTRACT

A strictly aerobic, Gram-stain positive, slightly halophilic strain, designated SCSIO 04524(T), was isolated from a deep sea sediment sample collected from the northern South China Sea at a depth of 3415 m. The isolate slightly embedded into the medium after 72 h incubation at 30 °C. Growth was found to occur on media with 0-10 % NaCl but extremely weak growth occurred without supplying NaCl. The predominant menaquinone was determined to be MK-7. The major cellular fatty acid identified was iso-C15:0. The diagnostic polar lipids were determined to be diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content was determined to be 38 mol%. 16S rRNA gene sequences analysis showed that this strain had the highest similarities with Bacillus carboniphilus JCM 9731(T) (94.7 %) and Bacillus endophyticus 2DT(T) (94.3 %). Phylogenetic analysis revealed that strain SCSIO 04524(T) formed a distinct lineage with Bacillus chungangensis CAU 348(T) and B. carboniphilus JCM 9731(T). Physiological characteristics including utilization of sole nitrogen and carbon sources, and chemotaxonomic properties of cellular fatty acids and polar lipids could readily distinguish strain SCSIO 04524(T) from its most closely related species. Based on this polyphasic taxonomic data, a new species, Bacillus oceani sp. nov., is proposed, with the type strain SCSIO 04524(T) (=DSM 26213(T) = KCTC 33077(T)).


Subject(s)
Bacillus/classification , Bacillus/isolation & purification , Aerobiosis , Bacillus/genetics , Bacillus/physiology , Bacterial Typing Techniques , Base Composition , Carbon/metabolism , China , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Geologic Sediments/microbiology , Molecular Sequence Data , Nitrogen/metabolism , Phospholipids/analysis , Phylogeny , Quinones/analysis , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sodium Chloride/metabolism , Temperature
15.
Int J Syst Evol Microbiol ; 63(Pt 12): 4552-4556, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23912721

ABSTRACT

A novel filamentous bacterium, strain SCSIO 11153(T), was isolated from a sediment sample collected from the Indian Ocean (80° 03.099' E 01° 03.300' N) at a depth of 4593 m. Good growth was observed at 50-55 °C and pH 7.0 with 3 % NaCl. It formed ivory-white colonies with radial wrinkles. Aerial mycelium was absent on the media tested. Phenotypic characteristics and 16S rRNA gene sequence analysis indicated that strain SCSIO 11153(T) belonged to the family Thermoactinomycetaceae. It exhibited 96.4% and 96.2% 16S rRNA gene sequence similarities to Melghirimyces algeriensis NariEX(T) and Melghirimyces thermohalophilus Nari11A(T), respectively, while lower sequence similarity values (<95.4%) were observed between strain SCSIO 11153(T) and other species of genera in the family Thermoactinomycetaceae. The menaquinone type was MK-7. Major cellular fatty acids were iso-C15:0, anteiso-C15:0 and iso-C17:0. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain SCSIO 11153(T) was 52.6 mol%. On the basis of the genotypic and phenotypic characteristics, it is proposed that strain SCSIO 11153(T) represents a novel species of the genus Melghirimyces with the name Melghirimyces profundicolus sp. nov. The type strain is SCSIO 11153(T) ( = DSM 45787(T) = CCTCC AA 2012007(T) = NBRC 109068(T)).


Subject(s)
Bacillales/classification , Geologic Sediments/microbiology , Phylogeny , Seawater/microbiology , Bacillales/genetics , Bacillales/isolation & purification , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/genetics , Fatty Acids/chemistry , Indian Ocean , Molecular Sequence Data , Peptidoglycan/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistry
16.
Int J Syst Evol Microbiol ; 63(Pt 11): 4243-4247, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23811137

ABSTRACT

A Gram-stain-positive actinobacterium, designated strain SCSIO 15020(T), was isolated from sediment of the South China Sea, and characterized by using a polyphasic approach. The temperature range for growth was 24-60 °C, with optimal growth occurring at 50 °C. The pH range for growth was 6-10 (optimum pH 8-9). The NaCl concentration range for growth was 0-5 % (w/v). The peptidoglycan type was A4α. Polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown polar lipid. The major menaquinone was MK-8(H4); MK-7(H4) was present as a minor component. The major fatty acids (>5 %) were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0. The DNA G+C content of strain SCSIO 15020(T) was 73.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 15020(T) belonged to the genus Georgenia, with the closest neighbours being Georgenia muralis 1A-C(T) (96.3 % similarity), Georgenia thermotolerans TT02-04(T) (95.7 %) and Georgenia ruanii YIM 004(T) (95.6 %). Based on evidence from the present polyphasic study, strain SCSIO 15020(T) is considered to represent a novel species of the genus Georgenia, for which the name Georgenia sediminis sp. nov. is proposed. The type strain is SCSIO 15020(T) ( = DSM 25884(T) = NBRC 108941(T)).


Subject(s)
Actinomycetales/classification , Geologic Sediments/microbiology , Phylogeny , Actinomycetales/genetics , Actinomycetales/isolation & purification , Bacterial Typing Techniques , Base Composition , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Molecular Sequence Data , Peptidoglycan/chemistry , RNA, Ribosomal, 16S/genetics , Seawater/microbiology , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistry
17.
Antonie Van Leeuwenhoek ; 103(5): 963-9, 2013 May.
Article in English | MEDLINE | ID: mdl-23314911

ABSTRACT

A Gram-positive bacterium, designated SCSIO 15042(T), was isolated from a sediment of the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew at 20-60 °C, pH 6.0-10.0 and it could grow with up to 10 % (w/v) NaCl. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and an unknown polar lipid. The only menaquinone was determined to be MK-7. The major fatty acids were identified as C16:1 ω7c/C16:1 ω6c, C16:0, iso-C15:0, anteiso-C15:0, and iso-C16:0. The DNA G+C content of strain SCSIO 15042(T) was determined to be 43.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SCSIO 15042(T) to the genus Bacillus. Levels of 16S rRNA gene sequence similarities between strain SCSIO 15042(T) and Bacillus herbersteinensis D-1-5a(T), Bacillus infantis SMC 4352-1(T), Bacillus novalis LMG 21837(T) and Bacillus drentensis LMG 21831(T) were 96.2, 96.2, 96.1 and 96.1 %, respectively. Based on the evidence of the present polyphasic study, strain SCSIO 15042(T) is considered to represent a novel species of the genus Bacillus, for which the name Bacillus abyssalis sp. nov. is proposed. The type strain is SCSIO 15042(T) (=DSM 25875(T) = CCTCC AB 2012074(T) = NBRC 109102(T)).


Subject(s)
Bacillus/classification , Bacillus/isolation & purification , Geologic Sediments/microbiology , Bacillus/genetics , Bacillus/physiology , Bacterial Typing Techniques , Base Composition , Cell Wall/chemistry , China , Cluster Analysis , Cytosol/chemistry , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Diaminopimelic Acid , Fatty Acids/analysis , Hydrogen-Ion Concentration , Molecular Sequence Data , Phospholipids/analysis , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sodium Chloride/metabolism , Temperature , Vitamin K 2/analysis
18.
Int J Syst Evol Microbiol ; 63(Pt 3): 893-899, 2013 Mar.
Article in English | MEDLINE | ID: mdl-22634702

ABSTRACT

An aerobic actinomycete, designated SCSIO 01299(T), was isolated from a deep-sea sediment collected from the northern South China Sea at a depth of 3258 m. The isolate was found to be a natural producer of the synthesized antitumour agent deoxynyboquinone and its three new derivatives, pseudonocardians A, B and C. A blast search based on almost-complete 16S rRNA gene sequences showed that strain SCSIO 01299(T) had high sequence similarities with members of the genus Pseudonocardia. The 16S rRNA gene sequence phylogenetic tree revealed that strain SCSIO 01299(T) was a member of the genus Pseudonocardia. Phenotypic analysis, chemotaxonomy and DNA-DNA relatedness could readily distinguish the isolate from established members in this genus. It was concluded that strain SCSIO 01299(T) represents a novel species, for which the name Pseudonocardia antitumoralis sp. nov. is proposed. The type strain is SCSIO 01299(T) ( = DSM 45322(T)  = CCTCC M 2011255(T)).


Subject(s)
Actinomycetales/classification , Geologic Sediments/microbiology , Phylogeny , Actinomycetales/genetics , Actinomycetales/isolation & purification , Bacterial Typing Techniques , China , DNA, Bacterial/genetics , Fatty Acids/analysis , Molecular Sequence Data , Nucleic Acid Hybridization , Quinones/metabolism , RNA, Ribosomal, 16S/genetics , Seawater/microbiology , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/analysis , Water Microbiology
19.
Bioorg Med Chem Lett ; 22(23): 7265-7, 2012 Dec 01.
Article in English | MEDLINE | ID: mdl-23079524

ABSTRACT

Two new indole diketopiperazines, namely luteoalbusins A-B (1-2), along with eight known ones (3-10), were isolated from the fungus Acrostalagmus luteoalbus SCSIO F457 originated from deep-sea sediment. Their structures were determined by 1D/2D NMR, MS, and CD data analyses. Each of these compounds was evaluated for their cytotoxic activities against SF-268, MCF-7, NCI-H460, and HepG-2 cell lines, and compounds 1-5 showed significant cytotoxicties against all four cancer cell lines. Moreover, new compounds 1 and 2 had more potent cytotoxicity than the other ones and cisplatin.


Subject(s)
Ascomycota/chemistry , Diketopiperazines/chemistry , Indoles/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Diketopiperazines/isolation & purification , Diketopiperazines/toxicity , Drug Screening Assays, Antitumor , Hep G2 Cells , Humans , Indoles/isolation & purification , Indoles/toxicity , MCF-7 Cells , Magnetic Resonance Spectroscopy , Molecular Conformation , Oceans and Seas
20.
Antonie Van Leeuwenhoek ; 102(1): 157-62, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22456792

ABSTRACT

A novel actinobacterium, designated strain SCSIO 10480(T), was isolated from a gorgonian coral sample of Anthogorgia sp. Phylogenetic and phenotypic properties of the organism supported that it belonged to the genus Janibacter. Phylogenetic analysis indicated that the levels of 16S rRNA gene sequence similarity between strain SCSIO 10480(T) and other type strains of recognized members of the genus Janibacter were 96.0-97.8 %. Growth in the presence of up to 17 % (w/v) NaCl and optimally at pH 9.0-10.0 was a distinctive characteristic of strain SCSIO 10480(T). Other biochemical and physiological properties and the fatty acid profile also differentiated the isolate from other members of Janibacter species. Based on the results obtained in this study, we propose that strain SCSIO 10480(T) should be classified within a novel species of the genus Janibacter, for which the name Janibacter alkaliphilus sp. nov. is proposed, with SCSIO 10480(T) (=CCTCC AB 2011027(T) = DSM 24723(T)) as the type strain.


Subject(s)
Actinomycetales/classification , Actinomycetales/isolation & purification , Anthozoa/microbiology , Actinomycetales/genetics , Actinomycetales/physiology , Animals , Aquatic Organisms/microbiology , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Hydrogen-Ion Concentration , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sodium Chloride/metabolism
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