ABSTRACT
Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are two predominant histological types of nonmelanoma skin cancer (NMSC), lacking effective early diagnostic markers. In this study, we assessed the diagnostic value of autoantibodies against p53, MMP-7, and Hsp70 in skin SCC and BCC. ELISA was performed to detect levels of autoantibodies in sera from 101 NMSC patients and 102 normal controls, who were recruited from the Cancer Hospital of Shantou University Medical College. A receiver operator characteristic curve was used to evaluate the diagnostic value. The serum levels of autoantibodies against p53, MMP-7, and Hsp70 were higher in NMSCs than those in the normal controls (all P < 0.01). The AUC of the three-autoantibody panel was 0.841 (95% CI: 0.788-0.894) with the sensitivity and specificity of 60.40% and 91.20% when differentiating NMSCs from normal controls. Furthermore, measurement of this panel could differentiate early-stage skin cancer patients from normal controls (AUC: 0.851; 95% CI: 0.793-0.908). Data from Oncomine showed that the level of p53 mRNA was elevated in BCC (P < 0.05), and the Hsp70 mRNA was upregulated in SCC (P < 0.001). This serum three-autoantibody panel might function in assisting the early diagnosis of NMSC.
Subject(s)
Autoantibodies/immunology , Biomarkers, Tumor/metabolism , HSP70 Heat-Shock Proteins/immunology , Matrix Metalloproteinase 7/immunology , Skin Neoplasms/diagnosis , Tumor Suppressor Protein p53/immunology , Adult , Female , Humans , Infant, Newborn , MaleABSTRACT
As a preliminary but important step in the investigation of the function of the new androgen-dependent DNA binding cytosol protein (C(3)P(4)) discovered by using gel retardation assay with a 31 bp fragment of DNA of the promoter of the rat ventral prostatic binding protein (PSBP) C(3) gene as a probe, a general survey was carried out on the tissue distribution of this protein in rat mice, rabbit and monkey of both sexes and also in some human pathological tissues with the following findings: (1) C(3)P(4) is present in all the male sex organs in different amount, but none in female sex organs. (2) C(3)P(4) has been found in the brain of all the species studied. (3) Its occurrence in other tissues are rare and show species variation, but no sex difference. (4) Of the samples collected from 8 different human pathological tissues, stomach cancer, both benign and malignant mammary tumors and three of the benign prostatic hyperplasia tissues revealed certain changes, the potential significance of these findings were discussed.