ABSTRACT
OBJECTIVE: Atopic dermatitis (AD) is an inflammatory skin disease. Naringenin (Nar) possesses an anti-inflammatory property. This paper attempts to discuss the functional mechanism of Nar in AD mice through the Janus kinase 2 (JAK2)/signal transducer and activation of transcription 3 (STAT3) pathway. METHODS: Mouse models of DNFB-induced AD were established and treated with Nar, followed by intraperitoneal injection with the JAK2/STAT3 pathway activator Coumermycin A1. Dermatitis severity was scored and the thickness of right ear was measured. The pathological changes in dorsal skin tissues were observed by HE staining. The number of infiltrated mast cells and eosinophilic granulocytes was counted by TB staining. The serum IgE level and levels of TNF-α, IL-6, IFN-γ, IL-12, and IL-5 in dorsal skin tissues were measured by ELISA. The levels of p-JAK2, JAK2, p-STAT3, and STAT3 were determined by Western blot. RESULTS: Nar decreased dermatitis scores and right ear thickness, alleviated skin lesions, and reduced the number of infiltrated mast cells and eosinophilic granulocytes in AD mice. The serum IgE level and levels of TNF-α, IL-6, IFN-γ, IL-12, and IL-5 in dorsal skin tissues of AD mice were diminished after Nar treatment in a dose-dependent manner. Nar inhibited the activation of the JAK2/STAT3 pathway. The activation of the JAK2/STAT3 pathway partially nullified the therapeutic function of Nar on AD mice. CONCLUSION: Nar protects mice from AD by inhibiting inflammation and promoting immune responses through the inhibition of the JAK2/STAT3 pathway.
Subject(s)
Dermatitis, Atopic , Flavanones , Mice , Animals , Dermatitis, Atopic/drug therapy , Dermatitis, Atopic/pathology , Interleukin-6 , Tumor Necrosis Factor-alpha , Janus Kinase 2/metabolism , Interleukin-5/adverse effects , Cytokines , Inflammation/drug therapy , Immunoglobulin E/adverse effects , Interleukin-12/adverse effectsABSTRACT
Spontaneous rupture of the bladder (SRUB) is an extremely rare emergency that can be misdiagnosed due to its non-specific clinical presentation, leading to patient death. This study aimed to summarize SRUB's clinical features, diagnosis, and treatment through our case. We report a case of a 39-year-old female with preoperative peritonitis and peritoneal effusion diagnosis. We performed an emergency laparoscopic surgery and found she had a ruptured bladder. She was treated with laparoscopic rupture repair and suprapubic cystostomy. She recovered well, was discharged after seven days, and had the cystostomy tube removed after five weeks.
