ABSTRACT
As they typically have limited direct contact, children's attitudes towards older adults have more opportunity to be shaped by other social influences such as their parents and children's literature. Children's books have been noted for their tendency to portray older adults in stereotypical ways and their tendencies to underrepresent older adults. We investigated how the portrayal of older adults as major versus supporting characters, as well as parents' age-related expectations were related to parental preference for children's books. We designed 24 children's book covers that depicted an older adult as a main character, a younger adult as a main character, or only children. One-hundred-seventy-five parents of children ages 0-12 rated their preference for the covers, their age-related expectations for the books' stories, and their personal aging expectations. Parents preferred covers featuring only children, and this preference was stronger for parents with more positive personal aging expectations. Cover preference was further predicted by age-related story expectations. When parents expected a book to conform to older-age stereotypes, they liked that particular cover less. Controlling for parents' age-related story expectations for each book resulted in near equal levels of preference for all types of book covers. Carefully designed children's books could provide an opportunity to increased vicarious intergenerational contact. These finding suggests that parents' interest in selecting these books for their children will be higher when they do not perceive the books to align with older-age stereotypes. Supplementary information: The online version contains supplementary material available at 10.1007/s12144-023-04298-6.
ABSTRACT
Immune checkpoint molecules are highly relevant as potential prognostic markers and therapeutic targets in malignant diseases. HVEM belongs to the TNF receptor family and provides stimulatory as well as inhibitory signals depending on the ligand. Abnormal HVEM expression has been described in various malignancies, but the role in AML is unknown. Here we report extensive data on HVEM surface protein expression analyzed by flow cytometry on bone marrow leukemic cells of 169 AML patients at diagnosis. An independent cohort of 512 AML patients was analyzed for HVEM mRNA expression in bone marrow samples by Affymetrix microarrays. Consistently for both cohorts and methods, we show that HVEM was differentially expressed and that expression levels were associated with defined genetic markers. HVEM expression was lower in cases with FLT3-ITD (p = 0.001, p < 0.001), with mutations in NPM1 (p = 0.001, p < 0.001) or with the combination of NPM1 mutation and FLT3 wild type (p = 0.049, p = 0.050), while a biallelic mutation in CEBPA correlated positively with higher HVEM expression (p = 0.015, p < 0.001). In a differential gene expression analysis, we found 13 genes including HOXA9, MEIS1 and MN1 that were closely associated with HVEM expression. Besides, four gene sets closely linked to immunity were enriched in HVEM (high) samples. Finally, high expression of HVEM was associated with a trend toward longer relapse-free survival. The results of this study provide new information on the potential significance of HVEM in AML.
