Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopy.

Computational super-resolution methods, including conventional analytical algorithms and deep learning models, have substantially improved optical microscopy. Among them, supervised deep neural networks have demonstrated outstanding performance, however, demanding abundant high-quality training data, which are laborious and even impractical to acquire due to the high dynamics of living cells. Here, we develop zero-shot deconvolution networks (ZS-DeconvNet) that instantly enhance the resolution of microscope images by more than 1.5-fold over the diffraction limit with 10-fold lower fluorescence than ordinary super-resolution imaging conditions, in an unsupervised manner without the need for either ground truths or additional data acquisition. We demonstrate the versatile applicability of ZS-DeconvNet on multiple imaging modalities, including total internal reflection fluorescence microscopy, three-dimensional wide-field microscopy, confocal microscopy, two-photon microscopy, lattice light-sheet microscopy, and multimodal structured illumination microscopy, which enables multi-color, long-term, super-resolution 2D/3D imaging of subcellular bioprocesses from mitotic single cells to multicellular embryos of mouse and C. elegans.

Multiple Sulfur Isotopic Evidence for Sulfate Formation in Haze Pollution.

The mechanism of sulfate formation during winter haze events in North China remains largely elusive. In this study, the multiple sulfur isotopic composition of sulfate in different grain-size aerosol fractions collected seasonally from sampling sites in rural, suburban, urban, industrial, and coastal areas of North China are used to constrain the mechanism of SO2 oxidation at different levels of air pollution. The Δ33S values of sulfate in aerosols show an obvious seasonal variation, except for those samples collected in the rural area. The positive Δ33S signatures (0‰ < Δ33S < 0.439‰) observed on clean days are mainly influenced by tropospheric SO2 oxidation and stratospheric SO2 photolysis. The negative Δ33S signatures (-0.236‰ < Δ33S < ∼0‰) observed during winter haze events (PM2.5 > 200 µg/m3) are mainly attributed to SO2 oxidation by H2O2 and transition metal ion catalysis (TMI) in the troposphere. These results reveal that both the H2O2 and TMI pathways play critical roles in sulfate formation during haze events in North China. Additionally, these new data provide evidence that the tropospheric oxidation of SO2 can produce significant negative Δ33S values in sulfate aerosols.

The nuclear cytokine IL-37a controls lethal cytokine storms primarily via IL-1R8-independent transcriptional upregulation of PPARγ.

Cytokine storms are crucial in the development of various inflammatory diseases, including sepsis and autoimmune disorders. The immunosuppressive cytokine INTERLEUKIN (IL)-37 consists of five isoforms (IL-37a-e). We identified IL-37a as a nuclear cytokine for the first time. Compared to IL-37b, IL-37a demonstrated greater efficacy in protecting against Toll-like receptor-induced cytokine hypersecretion and lethal endotoxic shock. The full-length (FL) form of IL-37a and the N-terminal fragment, which is processed by elastase, could translocate into cell nuclei through a distinctive nuclear localization sequence (NLS)/importin nuclear transport pathway. These forms exerted their regulatory effects independent of the IL-1R8 receptor by transcriptionally upregulating the nuclear receptor peroxisome proliferator-activated receptor (PPARγ). This process involved the recruitment of the H3K4 methyltransferase complex WDR5/MLL4/C/EBPß and H3K4me1/2 to the enhancer/promoter of Pparg. The receptor-independent regulatory pathway of the nuclear IL-37a-PPARγ axis and receptor-dependent signaling by secreted IL-37a maintain homeostasis and are potential therapeutic targets for various inflammatory diseases, including sepsis.

Legacy phosphorus delays the accomplishment of expected phosphorus management object.

Legacy phosphorus (P) in watersheds continuously affects the water quality. The time lag between anthropogenic P input and algal bloom has made P dynamics prediction in aquatic ecosystems more challenging. Whether the legacy P in the Yangtze River Watershed (YRW) exceeds its storage threshold remains unknown, and the continuous impact of legacy P on the water quality has not been analyzed. This study aimed to evaluate variation trends (1970-2018) and influencing factors for accumulated P in the YRW under different economic development periods, quantitatively identify the watershed P storage threshold based on the two split line models and estimate the time required for the return of legacy P to the baseline level using an exponential decay process. The results showed that the P storage threshold of the YRW was surpassed due to intense anthropogenic activities, and the residual P still had an impact on aquatic ecosystems for a long time. The dissolved total P loadings may become the top priority to achieve better P management goals. The time lags for the legacy P restoration would require for about 1000 years to be exhausted. The legacy P in the YRW would continuously undermine the restoration efforts of the water quality. The combined effects of watershed P surplus reductions and depletion of residual P may become essential to better manage P in the future. We still need to strengthen our efforts to make soil legacy P more absorbed by crops and improve sewage treatment capacity to achieve sustainable development of YRW.

Attenuated airways inflammation and remodeling in IL-37a and IL-37b transgenic mice with an ovalbumin-induced chronic asthma.

BACKGROUND: Asthma is a common chronic respiratory disease characterized by airways inflammation, hyperresponsiveness and remodeling. IL-37, an anti-inflammatory cytokine, consists of five splice isoforms, that is, a-e. Although it has been previously shown that recombinant human IL-37b is able to inhibit airway inflammation and hyperresponsiveness in animal models of asthma, the effects and difference of other IL-37 isoforms, such as IL-37a on features of asthma are unknown. METHODS: Animal models of chronic asthma were established using IL-37a and IL-37b transgenic mice with C57BL/6J background and wild-type (WT) mice sensitized and nasally challenged with ovalbumin (OVA). Airway hyperresponsiveness was measured using FlexiVent apparatus, while histological and immunohistological stainings were employed to measure airways inflammation and remodeling indexes, including goblet cell metaplasia, mucus production, deposition of collagen, hypertrophy of airway smooth muscles and pulmonary angiogenesis. RESULTS: Compared to WT mice, both IL-37a and IL-37b transgenic mice had significant reduced airway hyperresponsiveness and the declined total numbers of inflammatory cells, predominant eosinophils into airways and lung tissues. Furthermore, all features of airways remodeling, including degrees of mucus expression, collagen deposition, hypertrophy of smooth muscles, thickness of airways and neovascularization markedly decreased in IL-37 transgenic mice compared with OVA-treated WT mice. CONCLUSION: Our data suggest that both IL-37a and IL-37b isoforms are able to not only ameliorate airways inflammation and airways hyperresponsiveness, but also greatly reduce airways structural changes of animal models of chronic asthma.

Source partitioning using phosphate oxygen isotopes and multiple models in a large catchment.

Excessive phosphorus (P) loadings cause major pollution concerns in large catchments. Quantifying the point and nonpoint P sources of large catchments is essential for catchment P management. Although phosphate oxygen isotopes (δ18O(PO4)) can reveal P sources and P cycling in catchments, quantifying multiple P sources in a whole catchment should be a research focus. Therefore, this study aimed to quantitatively identify the proportions of multiple potential end members in a typical large catchment (the Yangtze River Catchment) by combining the phosphate oxygen isotopes, land use type, mixed end-element model, and a Bayesian model. The δ18O(PO4) values of river water varied spatially from 4.9‰ to18.3‰ in the wet season and 6.0‰ to 20.9‰ in the dry season. Minor seasonal differences but obvious spatial changes in δ18O(PO4) values could illustrate how human activity changed the functioning of the system. The results of isotopic mass balance and the Bayesian model confirmed that controlling agricultural P from fertilizers was the key to achieving P emission reduction goals by reducing P inputs. Additionally, the effective rural domestic sewage treatment, development of composting technology, and resource utilization of phosphogypsum waste could also contribute to catchment P control. P sources in catchment ecosystems can be assessed by coupling an isotope approach and multiple-models.

Coupling isotopic signatures and partial extraction method to examine lead pollution in mangrove sediments.

Elevated lead (Pb) has been widely observed in mangrove sediments due to human activities, yet understanding the sources of Pb in these sediments and the factors influencing Pb accumulation is challenging. Here, we combined Pb isotopes with partial extraction methods to study Pb contamination levels in mangrove sediments from the eastern and western parts of the Maowei Sea, China. Our results showed that the Pb in the leachate and residual fraction was mainly from anthropogenic and natural sources, respectively. The use of 204Pb isotope analysis can reveal some overlooked differences between anthropogenic and natural sources. Calculation by Bayesian mixing model showed no significant difference in the total anthropogenic contribution between the two sites, but the relative contribution of each end member differed. The contribution of Pb/Zn ores was much higher in the eastern sites (30.9 ± 5.1%) than in the west (18.4 ± 5.5%), while that of agricultural activities was much lower in the east (5.2 ± 3.1%) than in the west (13.5 ± 4.6%). The elevated anthropogenic Pb accumulation in mangrove sediments was ascribed to organic matter. This study provides more data on Pb isotopic composition and new insights into Pb biogeochemistry in the mangrove environment.

Isotopic signatures unveil the lead sources and migration in surface mangrove sediments.

Mangrove sediments act as both sinks and secondary sources for lead (Pb), yet the sources, migration, and transformations of Pb in mangrove environments are poorly understood. In this study, Pb concentration in three mangrove sediments adjacent to different land-use types was evaluated. The Pb sources were quantitatively identified using Pb isotopes. Our data indicated minor Pb contamination in the mangrove sediments, possibly due to the relative lack of developed industry in this region. The Pb isotopic ratios suggested, on average, natural sources, coal combustion, agricultural activities, and traffic-related emissions respectively contributed approximately 61.4 %, 18.8 %, 14.0 %, and 5.8 % of the Pb accumulation in the mangrove sediments, suggesting that coal combustion and agriculture were important anthropogenic Pb sources. Significant relationships were observed between the 206Pb/207Pb ratios and total organic content (TOC) in mangrove sediments, which implied contrasting Pb cycling in two mangrove environments. We further suggested that organic matter and sulfur content significantly reduced Pb mobility and bioavailability in mangrove sediments. Our study provides isotopic method to investigate the Pb sources and migration in the mangrove environment.

Control mechanisms of water chemistry based on long-term analyses of the Yangtze River.

Long-term series data can provide a glimpse of the influence of natural and anthropogenic factors on water chemistry. However, few studies have been conducted to analyze the driving forces of the chemistry of large rivers based on long-term data. This study aimed to analyze the variations and driving mechanisms of riverine chemistry from 1999 to 2019. We compiled published data on major ions in the Yangtze River, one of the three largest rivers in the world. The results showed that Na+ and Cl- concentrations decreased with increasing discharge. Significant differences in riverine chemistry were found between the upper and middle-lower reaches. Major ion concentrations in the upper reaches were mainly controlled by evaporites, especially Na+ and Cl- ions. In contrast, major ion concentrations in the middle-lower reaches were mainly affected by silicate and carbonate weathering. Furthermore, human activities were the drivers of some major ions, notably SO42- ions associated with coal emissions. The increased major ions and total dissolved solids in the Yangtze River in the last 20 years were ascribed to the continuous acidification of the river and the construction of the Three Gorges Dam. Attention should be given to the impact of anthropogenic activities on the water quality of the Yangtze River.

Rare earth elements in soil around coal mining and utilization: Contamination, characteristics, and effect of soil physicochemical properties.

REEs are emerging contaminants, and soils nearby coal and coal ash with high REEs composition are vulnerable to REEs contamination. Besides, coal industry can alter surrounding soil characteristics. However, there is information paucity about REEs contamination and geochemical behaviors along with soil characteristics around coal industrial areas, which are essential for understanding their toxicity and mobilization. The study was conducted in soils surrounding Kriel coal-fired power plant (KCM) and Greenside coal mining in Witbank (GSCM), South Africa. Multivariate statistical analysis, pollution and fractionation indices, and BCR sequential extraction were applied. The ∑REEs in the soils were compared to ∑REEs abundance in the upper earth's crust (UEC), and slightly higher ∑REEs was found in KCM but slightly lower in GSCM. Generally, LREEs are abundant. The soil REEs were normalized using the Post-Archean Australian Shale (PAAS) and Eu and Gd in KCM and Gd in GSCM were >1. Contamination assessment revealed that the soils are slightly to moderately contaminated by REEs. ∑REEs in KCM was significantly correlated with soil particle sizes of 2.00-50.00 µm, Al2O3, Fe2O3, and MnO, while with 2.00-3.00 µm and Al2O3 in GSCM. Fractionation characteristics showed a positive Ce anomaly, with positive linear regressions with Fe2O3 and MnO. In contrast, a negative Eu anomaly was found with positive linear regressions with Al, Ca, and Mg-oxides. Oxidizable fractioned REEs accounted for 32.33% of the ∑REEs in GSCM and 35.85% in KCM, and their high EF suggests enrichment that could be due to coal mining and utilization. Most soil physicochemical properties appear to be negatively correlated with the exchangeable REEs. Overall, the soils are contaminated by REEs and the REEs characteristics are considerably influenced by major elements oxide, U, and Th contents. Therefore, more attention should be paid to REEs contamination and impacts around coal mining and utilization.

Impacts of mining activities on the spatial distribution and source apportionment of soil organic matter in a karst farmland.

Worldwide mining activities produce vast quantities of mine tailings, which pose a threat to soil quality, crop yields, and the regional environment in the adjacent agricultural soil, but little is known about the impact of mining activities on the SOM source and migration. In this study, soil samples of the topsoil (0-15 cm) and soil profiles (0-50 cm), as well as the potential sources samples (C3 plants, C4 plants and mining tailings) were collected from mine-contaminated karst farmland of four different pollution levels (NP, non-polluted; SP, slightly polluted; MP, moderately polluted; and HP, heavily polluted). Total organic carbon (TOC), total organic nitrogen (TON), and stable isotopic compositions (δ13Corg and δ15Norg) of soil and potential sources samples were determined. In the topsoil, the concentrations of TOC (1.9 ± 1.4 %) and TON (0.1 ± 0.1 %), and the value of δ13Corg (-25.4 ± 0.9 ‰) and δ15Norg (-3.6 ± 3.6 ‰), were not significantly different among HP, MP and SP farmland (P > 0.05). C3 plants (42.1 %-49.9 %) and mine tailings (32.3 %-40.1 %) were identified as the dominant source of topsoil SOM. In the soil profile, TOC%, TON%, δ13Corg, and δ15Norg were affected by soil depth and pollution level. TOC% and TON% in the soil profiles of NP changed slightly with soil depth, while that in the other soil profiles was decreased with the increasing of soil depth. The δ15Norg value in the SP soil dropped sharply when the soil depth was >15 cm, while that in the HP and MP soil was fluctuated and no obvious vertical pattern. Our findings provide valuable information regarding the impact of mining activities on SOM distribution and source apportionment in karst farmlands. The effects of mine tailings on SOM should be considered when the soil quality was estimated in the mine-grain composite area.

A comprehensive method of source apportionment and ecological risk assessment of soil heavy metals: A case study in Qingyuan city, China.

The study combined multiple models to provide a deeper understanding to soil heavy metal contamination and source information, which are essential for controlling pollution and reducing human health risks. In this study, the agricultural soils were collected from the Qingyuan City of China as an example. The multiple models (APCS/MLR, PMF, and GDM) were used to identify and quantitatively apportion the main sources of heavy metal pollution in the area. The results showed that Cu (56.4 %), Ni (70.9 %), B (44.5 %), and Cr (72.8 %) were associated with natural sources, such as soil parent material and soil-forming processes. However, Pb (41.2 %), Zn (61.8 %), Hg (67.0 %), and Cd (69.6 %) were associated with agricultural activities, atmospheric deposition, vehicle exhaust emissions, and vehicle tires, while Mo, Se, and Mn were possibly derived from natural sources, including rock weathering and soil parent materials. Additionally, the network of environmental analysis revealed that soil microbes are far more sensitive to soil heavy metal pollution than herbivores, vegetation, and carnivores. This study can serve as a guideline for reducing the ecological and health risks associated with heavy metals in soil by controlling their preferential sources. Environmental implication Combining multiple models is more effective approach to wide understanding of heavy metal contamination and source information, which is essential for controlling pollution and reducing human health risks. Based on multiple models (APCS/MLR, PMF, and GDM) and network environ analysis, a comprehensive method for apportioning soil heavy metal sources and assessing ecological risk had been provided. Further, the present study can be a guideline for reducing ecological and health risks by heavy metals in soil by controlling preferential sources.

Foliar cadmium uptake, transfer, and redistribution in Chili: A comparison of foliar and root uptake, metabolomic, and contribution.

Atmospheric deposition is an essential cadmium (Cd) pollution source in agricultural ecosystems, entering crops via roots and leaves. In this study, atmospherically deposited Cd was simulated using cadmium sulfide nanoparticles (CdSN), and chili (Capsicum frutescens L.) was used to conduct a comparative foliar and root experiment. Root and foliar uptake significantly increased the Cd content of chili tissues as well as the subcellular Cd content. Scanning electron microscopy and high-resolution secondary ion mass spectrometry showed that Cd that entered the leaves via stomata was fixed in leaf cells, and the rest was mainly through phloem transport to the other organs. In leaf, stem, and root cell walls, Cd signal intensities were 47.4%, 72.2%, and 90.0%, respectively. Foliar Cd uptake significantly downregulated purine metabolism in leaves, whereas root Cd uptake inhibited stilbenoid, diarylheptanoid, and gingerol biosynthesis in roots. Root uptake contributed 90.4% Cd in fruits under simultaneous root and foliar uptake conditions attributed to xylem and phloem involvement in Cd translocation. Moreover, root uptake had a more significant effect on fruit metabolic pathways than foliar uptake. These findings are critical for choosing pollution control technologies and ensuring food security.

Slight transition in Chinese atmospheric Pb isotopic fingerprinting due to increasing foreign Pb.

Atmospheric lead (Pb) pollution negatively affects human health and ecosystem, and extensive research is required to identify its sources and develop robust mitigation methods. In this study, the concentration and isotopic composition of Pb in fine particulate matter (PM2.5) at five sites in the China's Beijing-Tianjin-Hebei (BTH) region were analyzed. The results showed that the Pb concentration in the BTH region declined along the northwest direction in winter owing to the East Asian monsoon. Pb isotopic signatures confirmed that anthropogenic activities significantly contributed to Pb pollution, compared with natural sources. With the increasing import of foreign Pb (with a relatively lower 208Pb/206Pb ratio) to China, we hypothesized that the unique isotopic signature of Pb in Chinese aerosols may decline over time. Therefore, the application of the isotopic approach for quantifying Pb transported from China should be carefully appraised in future research to provide a realistic estimate of the contribution of local sources and the transboundary effect consistent with air mass trajectories analysis. This study provides a theoretical reference for supporting the utilization of Δ208Pb values for better clarify the transboundary impact of Pb pollution and to reduce international disputes.

Tracing the transboundary transport of atmospheric Particulate Bound Mercury driven by the East Asian monsoon.

Taking Beijing-Tianjin-Hebei (BTH) with severe atmospheric mercury (Hg) and PM2.5 pollution as a typical region, this study clarified the characteristics and transboundary transport of atmospheric Particulate Bound Mercury (PBM2.5) affected by the East Asian monsoon. Five sampling sites were conducted in rural, suburban, urban, industrial, and coastal areas of BTH from northwest to southeast along the East Asian monsoon direction. PBM2.5 showed increasing concentrations from northwest to southeast and negative δ202Hg values, indicating significant contributions from anthropogenic sources. However, the mean Δ199Hg values of PBM2.5 at the five sites were significantly positive, probably triggered by the photoreduction of Hg(II) during long-range transport driven by the East Asian monsoon. Apart from local anthropogenic emissions as the primary sources, the transboundary transport of PBM2.5, driven by west and northwest air masses originating in Central Asia and Russia, contributed significantly to the PBM2.5 pollution of BTH. Moreover, these air masses reaching BTH would carry elevated PBM2.5 concentrations further transported to the ocean by the East Asian monsoon. In contrast, the southeast air masses transported from the ocean by the East Asian monsoon in summer diluted inland PBM2.5 pollution. This study provides insight into the atmospheric Hg circulation affected by the East Asian monsoon.

Influence of arbuscular mycorrhizal fungi on bioaccumulation and bioavailability of As and Cd: A meta-analysis.

Increasing industrial activity has led to a growing risk of arsenic (As) and cadmium (Cd) accumulations and biomagnifications in plants and humans. Arbuscular mycorrhizal fungi (AMF) have been extensively studied as a soil amendment owing to their capability to reduce the accumulation of As and Cd in plant tissues. However, a quantitative and data-based consensus has yet to be reached on the effect of AMF on As and Cd bioaccumulation and bioavailability. Here, a meta-analysis was conducted to quantitatively evaluate the impact of AMF using 1430 individual observations from 194 articles. The results showed that AMF inoculation caused a decrease in shoot and root As and Cd accumulation compared to control, and the reduction rates were affected by experimental duration, P fertilizer, AMF species, plant family, plant lifecycle, and soil properties. Intermediate experimental duration (lasting 56-112 days) and no P fertilizer favored AMF to reduce the shoot As and root Cd accumulation. Compared to other plant families, the reduction in As and Cd accumulation in legumes was the greatest, following AMF inoculation. The soils with alkaline, high organic carbon (OC), and low available phosphorus (AP) appeared to be more favorable for AMF to reduce As accumulation in plant tissues, while soils with low AP were more conducive to reducing the Cd accumulation in plant tissues. In addition, AMF inoculation increased pH (1.92%), OC (6.27%), easily-extractable glomalin-related soil protein (EE-GRSP) (29.36%), and total glomalin-related soil protein (T-GRSP) (29.99%), and reduced bioavailable As (0.52%) and Cd (2.35%) in soils compared to control. Overall, the meta-analysis provides valuable guidelines for the optimal use of AMF in different plant-soil systems.

Early diagenetic control on the enrichment and fractionation of rare earth elements in deep-sea sediments.

The rare earth elements and yttrium (REY) in bioapatite from deep-sea sediments are potential proxies for reconstructing paleoenvironmental conditions. However, the REY enrichment mechanism and the reliability of this tracer remain elusive because of the lack of key information from ambient pore water. Here, we report high-resolution geochemical data for pore water, bottom water, and bioapatite from deep-sea sites in the western Pacific. Our results reveal that the benthic flux of REY from the deep sea is less substantial than from the shallow marine realm, resulting in REY-rich sediment. The depth distribution of REY in pore water is opposite to that of bioapatite, and REY patterns and neodymium isotopic compositions are not uniformly distributed within bioapatite. These results indicate alteration of REY and neodymium isotopic compositions during early diagenesis. Therefore, we infer that REY from bioapatite are not robust recorders of the deep marine environment through Earth's history.

Broadband Nonlinear Optical Absorption Induced by Bandgap Renormalization in CVD-Grown Monolayer MoSe2.

Optical modulation on ultrashort time scales is both of central importance and an essential operation for applications in photonics and optoelectronics. Here, with a giant bandgap renormalization due to a high density of carrier injected by a femtosecond pulse, we realize an expected broadband saturable absorption in chemical vapor deposition grown monolayer transition-metal dichalcogenide MoSe2. Our findings reveal the band edge shift from ∼1.53 to ∼0.52 eV under the pump excitation of 0.80 eV, which is induced by the nonequilibrium occupation of electron-hole states after a Mott transition as well as the increase of carrier temperature.

Characteristics of cadmium translocation and isotope fractionation in Ricinus communis seedlings: Effects from split/cut-root and limited nutrients.

Studying cadmium (Cd) transport in plants will improve the current understanding of Cd tolerance mechanisms. Due to the influence of analytical techniques, the application of Cd isotopes in plants is still in its early stages. Therefore, the relationships between Cd isotope fractionation and Cd translocation in plants remain unclear. In this study, we cultured Ricinus communis in hydroponic solutions during split/cut-root experiments and limited and infinite nutrient experiments. To understand the Cd transport process, the Cd2+ and other ion concentrations in different tissues (i.e., roots, stems, and leaves) and nutrient solutions, Cd isotope composition and the soluble protein in tissues were measured. The results showed that although significant effects were evident in the top leaves, the principal roots had less pronounced effects on Cd2+ translocation in the stems. Moreover, Cd underwent homolateral transport before it was translocated from the principal roots to the leaves on the side without Cd. It was apparent that the stems were responsible for translocating Cd2+ in plants. In addition, the continuous supply of high Cd2+ concentrations inhibited the growth of the top leaves, while in low Cd2+ concentrations, it was gradually transferred to the top leaves. Moreover, the tissues of R. communis were enriched with lighter Cd isotopes compared with the solutions. The clear differences between the Cd isotope fractionation of leaves under infinite and limited nutrient experiments may be attributed to plant growth and Cd uptake rates. This study provides important information for understanding Cd2+ translocation in R. communis and furthers our understanding of its tolerance and hyperaccumulation.