ABSTRACT
OBJECTIVE: To investigate the value of combined tests of serum Golgi protein-73, alpha-fetoprotein- L3 and Tat-interacting protein-30 in the diagnosis of hepatitis B virus related cirrhosis and hepatocellular carcinoma. METHODS: The cross-sectional study was conducted at Yuebei People's Hospital, Guangdong, China, from January to October 2017, and comprised hepatitis B patients and healthy controls. Serum Golgi protein-73, alpha-fetoprotein-L3 and Tat-interacting protein-30 levels in both groups were detected by enzyme-linked immunosorbent assay (ELISA). Alpha-fetoprotein-L3 was separated and quantified by electrochemiluminescence immunoas says and the percentage of alpha-fetoprotein-L3 to alphafetoprotein was calculated. RESULTS: Of the 721 subjects, 525(%) were patients and 196(%) were healthy controls. Among the patients, 271(%) had chronic hepatitis B, 161(%) had liver cirrhosis and 93(%) had hepatocellular carcinoma. Serum Golgi protein-73, alpha-fetoprotein-L3 and Tat-interacting protein-30 levels were significantly different in the hepatocellular carcinoma patients compared to controls, and those with chronic hepatitis and liver cirrhosis (p<0.01 each). The sensitivity and specificity of the combined detection of the three serum levels for diagnosing cirrhosis were 78.26% and 86.72%. The corresponding values for diagnosing hepatocellular carcinoma were 86.02% and 92.51%. CONCLUSIONS: Combined detection of Golgi protein-73, alpha fetoprotein-L3 and Tat-interacting protein was found to have the potential to improve diagnostic accuracy.
Subject(s)
Acetyltransferases/blood , Carcinoma, Hepatocellular/diagnosis , Hepatitis B, Chronic/blood , Liver Cirrhosis/diagnosis , Liver Neoplasms/diagnosis , Membrane Proteins/blood , Transcription Factors/blood , alpha-Fetoproteins/metabolism , Adult , Aged , Carcinoma, Hepatocellular/blood , Carcinoma, Hepatocellular/etiology , Case-Control Studies , Female , Hepatitis B, Chronic/complications , Humans , Liver Cirrhosis/blood , Liver Cirrhosis/etiology , Liver Neoplasms/blood , Liver Neoplasms/etiology , Male , Middle Aged , Protein Isoforms/blood , Sensitivity and SpecificityABSTRACT
Objective To discuss the clinical value of high-sensitivity C-Reactive protein ( hs-CRP) and Interlukin-6 (IL-6) on patients with diabetes mellitus and athero sclerosis. Methods Serum hs-CRP is measured through particle enhanced immuoturbidimetric method by using analysis instrument 7200 . LDL-C is examined by one-step method. IL-6 is measured by double antibody assay. Results (1) Diabetic with higer LDL-C: the density of serum hs-CRP is (6. 81 ?3. 32) mg/L. IL-6 is (17. 2 ?5. 6) mg/L . Both are remarkably positive related. related coefficient r = 0. 61 ;P 0. 05). Conclusion For diabetic serum IL-6 plays roles in inducing the synthetic of CRP when there is an acute liver and also plays roles in the pathologic process of diabetes athero sclerosis.