Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 22
Filter
Add more filters











Publication year range
1.
Plant Mol Biol ; 102(1-2): 39-54, 2020 Jan.
Article in English | MEDLINE | ID: mdl-31807981

ABSTRACT

KEY MESSAGE: Arabidopsis ETHYLENE RESPONSE FACTOR12 (ERF12), the rice MULTIFLORET SPIKELET1 orthologue pleiotropically affects meristem identity, floral phyllotaxy and organ initiation and is conserved among angiosperms. Reproductive development necessitates the coordinated regulation of meristem identity and maturation and lateral organ initiation via positive and negative regulators and network integrators. We have identified ETHYLENE RESPONSE FACTOR12 (ERF12) as the Arabidopsis orthologue of MULTIFLORET SPIKELET1 (MFS1) in rice. Loss of ERF12 function pleiotropically affects reproductive development, including defective floral phyllotaxy and increased floral organ merosity, especially supernumerary sepals, at incomplete penetrance in the first-formed flowers. Wildtype floral organ number in early formed flowers is labile, demonstrating that floral meristem maturation involves the stabilisation of positional information for organogenesis, as well as appropriate identity. A subset of erf12 phenotypes partly defines a narrow developmental time window, suggesting that ERF12 functions heterochronically to fine-tune stochastic variation in wild type floral number and similar to MFS1, promotes meristem identity. ERF12 expression encircles incipient floral primordia in the inflorescence meristem periphery and is strong throughout the floral meristem and intersepal regions. ERF12 is a putative transcriptional repressor and genetically opposes the function of its relatives DORNRÖSCHEN, DORNRÖSCHEN-LIKE and PUCHI and converges with the APETALA2 pathway. Phylogenetic analysis suggests that ERF12 is conserved among all eudicots and appeared in angiosperm evolution concomitant with the generation of floral diversity.


Subject(s)
Arabidopsis Proteins/classification , Arabidopsis/growth & development , DNA-Binding Proteins/classification , Flowers/growth & development , Gene Expression Regulation, Plant , Homeodomain Proteins/classification , Phylogeny , Plant Development/physiology , Arabidopsis/genetics , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Flowers/genetics , Flowers/metabolism , Gene Regulatory Networks , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Inflorescence/metabolism , Meristem/genetics , Meristem/growth & development , Meristem/metabolism , Mutation , Open Reading Frames/genetics , Oryza/genetics , Oryza/growth & development , Oryza/metabolism , Phenotype , Plant Development/genetics , Plant Proteins/genetics , Plant Proteins/metabolism , Sequence Alignment , Transcription Factors , Transcriptome
2.
J Exp Bot ; 68(13): 3457-3472, 2017 06 15.
Article in English | MEDLINE | ID: mdl-28859377

ABSTRACT

The biphasic floral transition in Arabidopsis thaliana involves many redundant intersecting regulatory networks. The related AP2 transcription factors DORNRÖSCHEN (DRN), DORNRÖSCHEN-LIKE (DRNL), and PUCHI individually execute well-characterized functions in diverse developmental contexts, including floral development. Here, we show that their combined loss of function leads to synergistic floral phenotypes, including reduced floral merosity in all whorls, which reflects redundant functions of all three genes in organ initiation rather than outgrowth. Additional loss of BLADE-ON-PETIOLE1 (BOP1) and BOP2 functions results in the complete conversion of floral meristems into secondary inflorescence shoots, demonstrating that all five genes define an essential regulatory network for establishing floral meristem identity, and we show that their functions converge to regulate LEAFY expression. Thus, despite their largely discrete spatiotemporal expression domains in the inflorescence meristem and early floral meristem, PUCHI, DRN, and DRNL interdependently contribute to cellular fate decisions. Auxin might represent one potential non-cell-autonomous mediator of their gene functions, because PUCHI, DRN, and DRNL all interact with auxin transport and biosynthesis pathways.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Arabidopsis/growth & development , Arabidopsis/genetics , Flowers/growth & development , Gene Expression Regulation, Plant , Meristem/growth & development , Arabidopsis/metabolism , Flowers/genetics , Indoleacetic Acids/metabolism , Meristem/genetics , Organogenesis, Plant , Phylogeny , Transcription Factors/genetics , Transcription Factors/metabolism
3.
J Exp Bot ; 65(12): 3097-110, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24744428

ABSTRACT

In the Arabidopsis inflorescence meristem (IM), auxin is considered a prepatterning signal for floral primordia, whereas a centripetal mode of positional information for floral organ identity is inherent to the ABCE model. However, spatio-temporal patterns of organ initiation in each whorl at the earliest initiation stages are largely unknown. Evidence suggests that initial flower development occurs along an abaxial/adaxial axis and conforms to phytomer theory. Use of the founder cell marker DORNRÖSCHEN-LIKE (DRNL) as a tool in leafy, puchi, and apetala 1 cauliflower mutant backgrounds suggests that bract founder cells are marked at the IM periphery. The DRNL transcription domain in the wild-type IM is spatially discrete from DR5 expression, suggesting that bract initiation is independent of canonical auxin response. When bracts develop in lfy and puchi mutant floral primordia the initiation of lateral sepals precedes the specification of medial sepals compared with wild type, showing an interplay between bract and abaxial sepal founder cell recruitment. In the perianthia (pan) mutant background, DRNL expression indicates that a radial outer whorl arrangement derives from splitting of sepal founder cell populations at abaxial and adaxial positions. This splitting of incipient sepal primordia is partially dependent on PRESSED FLOWER (PRS) activity and implies that sepal specification is independent of WUSCHEL and CLAVATA3 expression, as both marker genes only regain activity in stage-2 flowers, when patterning of inner floral organs switches to a centripetal mode. The transition from an initially abaxial/adaxial into a centripetal patterning programme, and its timing represent an adaptive trait that possibly contributes to variation in floral morphology, especially unidirectional organ initiation.


Subject(s)
Arabidopsis/growth & development , Arabidopsis/metabolism , Indoleacetic Acids/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Flowers/genetics , Flowers/growth & development , Flowers/metabolism , Meristem/genetics , Meristem/growth & development , Meristem/metabolism , Polymerase Chain Reaction , Transcription Factors/genetics , Transcription Factors/metabolism
4.
Plant Cell Rep ; 32(1): 45-59, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23011125

ABSTRACT

KEY MESSAGE : Transgenic DRN::erGFP and DRNL::erGFP reporters access the window from explanting Arabidopsis embryos to callus formation and provide evidence for the acquisition of shoot meristem cell fates at the microcalli surface. The DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) genes encode AP2-type transcription factors, which are activated shortly after fertilisation in the zygotic Arabidopsis embryo. We have monitored established transgenic DRN::erGFP and DRNL::erGFP reporter lines using live imaging, for expression in embryonic suspension cultures and our data show that transgenic fluorophore markers are suitable to resolve dynamic changes of cellular identity at the surface of microcalli and enable fluorescence-activated cell sorting. Although DRN::erGFP and DRNL::erGFP are both activated in surface cells, their promoter activity marks different cell identities based on real-time PCR experiments and whole transcriptome microarray data. These transcriptome analyses provide no evidence for the maintenance of embryogenic identity under callus-inducing high-auxin tissue culture conditions but are compatible with the acquisition of shoot meristem cell fates at the surface of suspension calli.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/cytology , Arabidopsis/embryology , Imaging, Three-Dimensional , Promoter Regions, Genetic/genetics , Seeds/cytology , Transcription Factors/genetics , Arabidopsis/genetics , Arabidopsis Proteins/metabolism , Flow Cytometry , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Green Fluorescent Proteins/metabolism , Protoplasts/metabolism , Recombinant Fusion Proteins/metabolism , Seeds/metabolism , Suspensions , Transcription Factors/metabolism , Transcriptome/genetics , Transgenes/genetics
5.
Gene ; 271(2): 131-42, 2001 Jun 27.
Article in English | MEDLINE | ID: mdl-11418234

ABSTRACT

We will describe gene expression patterns in the maize caryopsis, which provide clues to developmental decisions and questions in the embryo and endosperm. The emphasis will be on the development of the root/shoot axis, which is the main achievement of plant embryogenesis. Data obtained in the vegetative seedling are included as far as they may be relevant to the elaboration of the shoot/root axis. Development of the embryo will be briefly compared to endosperm as both seed compartment exhibit pronounced differences.


Subject(s)
Gene Expression Profiling , Seeds/genetics , Zea mays/genetics , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Seeds/growth & development , Zea mays/embryology
6.
Nucleic Acids Res ; 28(18): 3542-50, 2000 Sep 15.
Article in English | MEDLINE | ID: mdl-10982874

ABSTRACT

The PHD finger, a Cys(4)-His-Cys(3) zinc finger, is found in many regulatory proteins from plants or animals which are frequently associated with chromatin-mediated transcriptional regulation. We show here that the PHD finger activates transcription in yeast, plant and animal cells. In plant homeodomain transcription factors the PHD finger is combined with an upstream leucine zipper. Both domains together form a highly conserved 180 amino acid region called the ZIP/PHDf motif and transcriptional activity of the PHD finger is masked when embedded in this motif. Our results indicate that the ZIP/PHDf domain is a potential regulatory domain of PHDf-HD proteins. The leucine zipper upstream of the PHD finger interacts with 14-3-3GF14 mu from Arabidopsis thaliana and 14-3-3GF14-12 from maize via a leucine zipper conserved in helix 4 of various 14-3-3 proteins from plants and animals. PHD-type plant homeodomain proteins consequently may represent potential targets of 14-3-3 signalling.


Subject(s)
Gene Expression Regulation , Leucine Zippers , Proteins/metabolism , Transcriptional Activation , Tyrosine 3-Monooxygenase , Zinc Fingers/physiology , 14-3-3 Proteins , Animals , Arabidopsis/genetics , Arabidopsis/physiology , Escherichia coli , Fungal Proteins/genetics , Gene Expression Regulation, Fungal , Gene Expression Regulation, Plant , Homeodomain Proteins/genetics , Plant Proteins/genetics , Recombinant Fusion Proteins/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/physiology , Transcription Factors/genetics , Transcription Factors/physiology , Zebrafish
7.
Dev Genes Evol ; 210(6): 300-10, 2000 Jun.
Article in English | MEDLINE | ID: mdl-11180835

ABSTRACT

Using confocal laser scanning microscopy we have characterized early and intermediate stages of maize wild-type embryogenesis and compared to mutant development of four different embryo-specific mutations, emb*-8518, emb*-8521, emb*-8537, and emb*-8542. Confocal laser scanning microscopy is well suited to study embryo development in maize in a nondisruptive manner from shortly after fertilization to late stages in embryogenesis. The analysis of the mutant morphology indicated that two of the recessive mutations, emb*-8518 and emb*-8521, cause an early developmental arrest in the proembryo/early transition stage: mutant embryos are unable to enter the morphogenetic phase of embryogenesis. In contrast, homozygous emb*-8537, and emb*-8542 embryos progress at least to the coleoptilar stage and sometimes establish a functional shoot meristem that can determine leaf primordia. The morphological characterization of mutants was confirmed by analysis of the expression pattern of three different marker genes: Lipid transfer protein 2, Zea mays Outer Cell Layer 1, and Knotted 1. Our data indicate that both emb*-8518 and emb*-8521 mutant embryos are impaired in restriction of ZmOCL1 transcripts to the embryonic protoderm and therefore fail to establish a normal radial organization. In contrast, emb*-8537 and emb*-8542 embryos exhibit the wild-type pattern and proceed in development to the formation of a shoot apical meristem and the establishment of bilateral symmetry.


Subject(s)
Mutation , Seeds/growth & development , Zea mays/genetics , Base Sequence , DNA Primers , Homozygote , Phenotype , Seeds/metabolism
8.
Plant Mol Biol ; 41(5): 615-25, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10645721

ABSTRACT

Elucidation of the exon/intron structure of the maize Zmhox1a homeobox gene revealed two small introns in the homeodomain. Both intron positions are conserved in animal counterparts encoded in the metazoan homeobox gene clusters and thus may indicate a common ancestor. The transcription start of the Zmhox1a gene has been localized far from the protein-coding region. Two distal untranslated leading exons are alternatively spliced to either the Zmhox1a coding exons or an unrelated open reading frame comprising two exons located internally of the large second Zmhox1a intron. Due to significant homology to the C-terminus of the Mutator transposase this alternative gene product was named Trap (transposon-associated protein). Splice site selection may involve two sequence elements conserved at the splice acceptor sites in front of the Zmhox1a and Trap protein-coding regions. The translation of a mRNA species devoid of exon 3 which encodes the Zmhox1a transcription start codon may give rise to an N-terminal deletion polypeptide, deltaZmhox1a. Ectopic expression experiments in transgenic tobacco indicate a putative function distinct from the full-length Zmhox1a protein.


Subject(s)
Alternative Splicing , Bacterial Proteins , Exons/genetics , Homeodomain Proteins/genetics , Plant Proteins/genetics , Promoter Regions, Genetic , RNA-Binding Proteins/genetics , Transcription Factors/genetics , Zea mays/genetics , Amino Acid Sequence , Base Sequence , Blotting, Northern , DNA, Plant/chemistry , DNA, Plant/genetics , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Genes, Homeobox/genetics , Genes, Plant/genetics , Genetic Variation , Homeodomain Proteins/physiology , Molecular Sequence Data , Plant Proteins/physiology , Protein Isoforms/genetics , Protein Isoforms/physiology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Analysis, DNA , Transcription, Genetic , Zea mays/chemistry
9.
Nucleic Acids Res ; 26(20): 4714-20, 1998 Oct 15.
Article in English | MEDLINE | ID: mdl-9753741

ABSTRACT

The maize ZmHox2a gene encodes two homeodomains which originated by a 699 bp duplication within an ancestral precursor. The sequences of the two ZmHox2a homeodomains are highly diverged in the N-terminal arm, while residues in the helical part have mostly been conserved. We show here that both ZmHox2a homeodomains are functional DNA-binding motifs but exhibit different target site specificities. CASTing experiments reveal a TCCT motif recognized by HD1 but a GATC tetranucleotide as the recognition sequence of HD2. Mutation of the central nucleotides in both tetranucleotide core motifs abolishes DNA binding. A domain swap experiment indicates that target site specificity is achieved in a combinatorial manner by the contributions of the diverged N-terminal arms together with the slightly different recognition helices. Computer modelling suggests that K47 and H54 in the recognition helices preferentially contact the bases at the 3'-terminus of the tetranucleotide target sequences.


Subject(s)
DNA-Binding Proteins/metabolism , Gene Duplication , Genes, Homeobox , Homeodomain Proteins/metabolism , Plant Proteins/metabolism , Zea mays/genetics , Amino Acid Sequence , Base Sequence , Binding Sites , Binding, Competitive , Computer Simulation , Consensus Sequence , DNA-Binding Proteins/genetics , Evolution, Molecular , Genes, Plant , Homeodomain Proteins/genetics , Molecular Sequence Data , Oligodeoxyribonucleotides/biosynthesis , Oligodeoxyribonucleotides/metabolism , Plant Proteins/genetics , Polymerase Chain Reaction , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/isolation & purification , Substrate Specificity , Zea mays/metabolism
10.
Mol Gen Genet ; 255(3): 248-57, 1997 Jul.
Article in English | MEDLINE | ID: mdl-9268015

ABSTRACT

The transcription start site of the maize Shrunken-1 (Sh-1) gene is sufficient for transcriptional initiation in the absence of other promoter elements and is recognized in vitro by the Initiator Binding Protein (IBP). We describe here in situ hybridization experiments performed on various maize tissues to quantify IBP transcription at the cellular level. IBP transcripts are found in the endosperm and in differentiating, enlarging cells of the shoot and the root of the maize seedling. This expression pattern overlaps with that of the Sh-1 gene and is therefore compatible with the hypothesis that the Sh-1 transcription start site is a target for IBP. In the developing spikelets of male and female inflorescences IBP transcript levels are very high in those organs that are later aborted when flowers become unisexual. Overexpression of the maize IBP1 gene product in transgenic tobacco causes a reduction in internodal elongation and effects gibberellin hormonal balance. The cellular expression pattern described here establishes IBP transcripts as an interesting molecular marker for enlarging, and presumably differentiating, cells released from the root or shoot apex.


Subject(s)
DNA-Binding Proteins/biosynthesis , Genes, Plant , Plant Proteins , Plant Roots/growth & development , Plant Shoots/growth & development , Zea mays/genetics , Cell Division , DNA-Binding Proteins/genetics , Gene Expression , In Situ Hybridization , Plant Roots/cytology , Plant Roots/genetics , Plant Shoots/cytology , Plant Shoots/genetics , Plants, Genetically Modified , Plants, Toxic , Tissue Distribution , Nicotiana/genetics , Transcription, Genetic
11.
Plant Cell ; 8(3): 349-62, 1996 Mar.
Article in English | MEDLINE | ID: mdl-8721746

ABSTRACT

The ZmHox1a and ZmHox1b (for Zea mays homeobox) genes map on chromosomes 8 and 6, respectively. Both homeobox genes encode proteins that show 91% similarity and are transcribed simultaneously in meristematic and proliferating cells of the maize plant. To gain insight into the biological function of these genes, both open reading frames were expressed in tobacco, under the control of the cauliflower mosaic virus 35S promoter. The resulting transgenic ZmHox1a or ZmHox1b plants showed identical phenotypic alterations that fall into three classes: size reduction, formation of adventitious shoots, and homeotic floral transformations. Approximately 30% of the ZmHox1-expressing plants grew to only one-third of the wild-type size, and most axillary buds gave rise to lateral shoots. Flower abnormalities included formation of petaloid stamens and development of secondary flowers within the primary gynoecium. Therefore, the ectopic expression of the maize ZmHox1 homeobox gene products affects the vegetative as well as the reproductive phase of tobacco plants. All phenotypic alterations were transmitted to the next generation.


Subject(s)
Genes, Homeobox , Genes, Plant , Homeodomain Proteins/biosynthesis , Nicotiana/physiology , Plant Proteins/biosynthesis , Plants, Toxic , Zea mays/genetics , Amino Acid Sequence , Chromosome Mapping , Gene Expression , Homeodomain Proteins/chemistry , Homeodomain Proteins/genetics , Molecular Sequence Data , Open Reading Frames , Phenotype , Plant Proteins/chemistry , Plant Proteins/genetics , Plants, Genetically Modified , Recombinant Fusion Proteins/biosynthesis , Sequence Homology, Amino Acid , Nicotiana/growth & development , Zea mays/physiology
12.
Plant Mol Biol ; 30(3): 439-53, 1996 Feb.
Article in English | MEDLINE | ID: mdl-8605297

ABSTRACT

Clones of two highly related genes, ZmHox2a and ZmHox2b (Zea mays homeobox), were isolated from maize embryo cDNA libraries by screening with the ZmHox1a homeobox sequence. The genes map to chromosomes 3 and 8, respectively, and encode mRNA transcripts of 6kb. The encoded proteins, ZmHox2a and b, share 84% sequence identity and exhibit a modular structure with several novel plant-specific protein domains. Interestingly, each ZmHox2a, gene product contains two complete homeodomains which, for Zmhox2a, were both shown to be functional DNA-binding motifs in vitro. Not only probes encoding the homeobox but also DNA fragments corresponding to other ZmHox2 domains hybridize to multiple bands in genomic Southern blots, indicating that related protein domains may be conserved in other maize genes. The ZmHox2a/b genes, therefore, are members of a novel and large class of maize genes, some of which can be expected to encode new transcription factors.


Subject(s)
Genes, Homeobox , Genes, Plant , Zea mays/genetics , Amino Acid Sequence , Animals , Base Sequence , Biological Evolution , Cell Nucleus/metabolism , Chromosome Mapping , DNA Primers , DNA, Plant , Homeodomain Proteins/genetics , Molecular Sequence Data , Multigene Family , Plant Proteins/genetics , Repetitive Sequences, Nucleic Acid
13.
Plant Mol Biol ; 25(3): 493-506, 1994 Jun.
Article in English | MEDLINE | ID: mdl-7914101

ABSTRACT

South-western screening of lambda gt11 expression library with a fragment of the Shrunken promoter containing the initiator element resulted in cloning of a novel maize gene. The encoded initiator-binding protein (IBP1) interacts at the transcription start site of the Shrunken promoter. Analysis of the 680 amino acid (aa) long polypeptide revealed a novel bipartite DNA-binding domain at the carboxyl terminus. In its amino-terminal part, it is weakly related to Myb R-repeats but the following basic region is also essential for DNA binding. A region of similarity to the conserved 2.1 and 2.2 motifs in bacterial sigma-factors is located close to the IBP1 amino terminus. Two putative nuclear localization signals are compatible with the presence of antigenically related polypeptides in nuclear protein extracts. The IBP1 gene was mapped to the long arm of chromosome 9 (9L095); a second highly related gene IBP2 is located on the short arm of chromosome 1 (1S014). Both genes encode proteins sharing 93% similarity and are transcribed with similar activity in different plant organs. A small 82 nucleotide intron in the IBP2 transcript is found unspliced to a variable degree in different tissues. Translation of this incompletely processed transcript would result in a truncated amino-terminal polypeptide lacking the DNA-binding domain.


Subject(s)
DNA-Binding Proteins/genetics , Genes, Plant/genetics , Nucleotidyltransferases/genetics , Plant Proteins , Promoter Regions, Genetic/genetics , Zea mays/genetics , Amino Acid Sequence , Base Sequence , Blotting, Southern , Blotting, Western , Cell Compartmentation , Cell Nucleus/chemistry , Chromosome Mapping , Cloning, Molecular , Glucose-1-Phosphate Adenylyltransferase , Molecular Sequence Data , Polymorphism, Restriction Fragment Length , RNA Splicing , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Transcription, Genetic
14.
EMBO J ; 11(9): 3367-74, 1992 Sep.
Article in English | MEDLINE | ID: mdl-1354614

ABSTRACT

A new maize homeobox gene was isolated by screening a lambda gt11 expression library with the 26 bp Shrunken feedback control element. Zmhox1a (Zea mays homeobox) is an unidentified maize gene mapping to the long arm of chromosome 8. It is a member of a new class of maize homeobox genes only distantly related to the Knotted class. The 3.1 kb Zmhox1a transcript can be detected in different maize tissues and encodes a polypeptide of 719 amino acids. Western blotting experiments detect the native 112 or 115 kDa protein in nuclear protein extracts, the nuclear localization being compatible with a function in transcriptional control. No Zmhox1a protein is detected in maize roots despite the presence of the Zmhox1a transcript; this may indicate a post-transcriptional control mechanism. A highly acidic central region of the Zmhox1a polypeptide implies a transcriptional activator function. The carboxy-terminal part of the maize homeodomain protein is related to the human Oct2 transcription factor, but homology to the POU specific domain is restricted to the POU-B subdomain. It was confirmed by DNase I footprinting experiments that DNA binding of the Zmhox1a homeodomain was at three sites flanking the TATA-box of the Shrunken promoter.


Subject(s)
DNA-Binding Proteins/genetics , Genes, Homeobox/genetics , Homeodomain Proteins , Plant Proteins/genetics , Regulatory Sequences, Nucleic Acid , Zea mays/genetics , Amino Acid Sequence , Base Sequence , Blotting, Southern , Chromosome Mapping , Cloning, Molecular , Consensus Sequence , DNA/metabolism , DNA-Binding Proteins/metabolism , Feedback , Molecular Sequence Data , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Plant Proteins/metabolism , RNA, Messenger/genetics , Sequence Homology, Nucleic Acid
15.
Plant Mol Biol ; 16(2): 199-207, 1991 Feb.
Article in English | MEDLINE | ID: mdl-1893097

ABSTRACT

Both exon 1 and intron 1 of the maize Shrunken-1 (Sh1) gene individually stimulate expression of reporter genes in transient gene expression experiments if present within the transcription unit. The Sh1 exon 1 mediates a 10-fold increase in activity when inserted at the 5' end of the bacterial chloramphenicol transacetylase (CAT) marker gene in both monocot and dicot protoplasts. The Sh1 intron 1 enhances chimeric gene expression in rice and maize protoplasts approximately 100-fold but inhibits CAT expression in tobacco protoplasts. In combination, the stimulatory effects of Sh1 exon 1 and intron 1 are multiplicative in monocot protoplasts resulting in a final enhancement of up to 1000-fold compared to the unmodified CAT or luciferase marker genes.


Subject(s)
Cloning, Molecular/methods , DNA , Exons , Regulatory Sequences, Nucleic Acid , Zea mays/genetics , Base Sequence , Chloramphenicol O-Acetyltransferase/genetics , Genetic Markers/genetics , Introns , Luciferases/genetics , Molecular Sequence Data , Plants, Toxic , Plasmids , Promoter Regions, Genetic , Protoplasts , Nicotiana/genetics
16.
EMBO J ; 9(11): 3447-52, 1990 Nov.
Article in English | MEDLINE | ID: mdl-2145150

ABSTRACT

The transcriptional activity of the Shrunken (Sh) promoter of Zea mays was monitored in transient expression assays using the neomycin phosphotransferase (NPT) II gene as a reporter in maize suspension protoplasts. Shortly after transfection, expression of this chimeric NPTII gene was negatively affected by high extracellular sucrose concentrations in the protoplast cultivation medium. However, 3-5 days after transfection an up to 405-fold increase in NPTII activity was observed. This could be blocked by dichlorobenzonitril (DCB) an inhibitor of cellulose biosynthesis. In the analysis of promoter deletions 20 bp upstream of the Sh transcription start site were sufficient to reproduce the expression profile and the activity of the full promoter. Surprisingly this start sequence does not include the natural TATA-box.


Subject(s)
Gene Expression Regulation , Glucosyltransferases/genetics , Promoter Regions, Genetic , Zea mays/genetics , Base Sequence , Cell Wall/metabolism , Cells, Cultured , Cloning, Molecular , Feedback , Gene Expression Regulation/drug effects , Molecular Sequence Data , Regulatory Sequences, Nucleic Acid , Sucrose/pharmacology , Transcription, Genetic/drug effects
17.
Mol Gen Genet ; 221(1): 94-101, 1990 Mar.
Article in English | MEDLINE | ID: mdl-2325634

ABSTRACT

We describe an analysis of protein-DNA interactions detectable with nuclear extracts prepared from maize kernels and DNA fragments from the immediate upstream region of the Shrunken gene from maize. The data demonstrate that sequences from position -235 to the transcription start are recognized by sequence specific nuclear proteins. In footprinting and competition experiments at least six different protein-DNA interactions can be distinguished within this upstream region. Two sequence related inverted repeat structures, 67 and 64 bp in length, cross compete for protein recognition.


Subject(s)
DNA-Binding Proteins/metabolism , Genes, Plant , Nucleoproteins/metabolism , Plant Proteins/metabolism , Transcription, Genetic , Zea mays/genetics , Base Sequence , Binding, Competitive , Molecular Sequence Data , Promoter Regions, Genetic
18.
Plant Cell Rep ; 8(3): 148-51, 1989 Mar.
Article in English | MEDLINE | ID: mdl-24233091

ABSTRACT

Experimental conditions influencing DNA uptake efficiency by maize protoplasts in polyethyleneglycol (PEG) mediated transfection experiments have been studied systematically. The data provide evidence that the extracellular DNA is precipitated efficiently by combined action of PEG together with divalent cations and DNA is taken up by the plant protoplasts in the precipitated form. The particle size is strongly effected by the pH of the PEG solution. At optimal pH 6- 6.5 a very fine and homogenous precipitate forms in presence of Ca(2+) and Mg(2+) ions and is efficiently incorporated by maize and rice protoplasts.

19.
Mol Gen Genet ; 212(2): 342-50, 1988 May.
Article in English | MEDLINE | ID: mdl-3405202

ABSTRACT

Nuclear proteins were extracted from isolated nuclei of immature maize kernels. The promoter region (1.5 kb) of the Shrunken gene, which is highly transcribed in the developing endosperm of the kernel, was scanned for protein-DNA interactions. Several promoter fragments showed protein-DNA complex formation in gel retardation experiments. Two different nucleo-protein complexes (MNP1 and MNP2) have been distinguished in competition and DNase I footprinting experiments. Both nuclear DNA-binding activities are able to recognize multiple sites distributed over a 1.5 kb upstream region of the Shrunken gene. Some of the binding sites established in the in vitro reconstitution experiments are located near to DNase I hypersensitive sites found in the promoter of the Shrunken gene (Frommer and Starlinger 1988).


Subject(s)
Genes , Mutation , Nuclear Proteins/metabolism , Plants/genetics , Promoter Regions, Genetic , Base Sequence , Molecular Sequence Data , Protein Binding , Transcription, Genetic , Zea mays/genetics
20.
Mol Gen Genet ; 205(3): 461-8, 1986 Dec.
Article in English | MEDLINE | ID: mdl-2436026

ABSTRACT

The Shrunken gene, located on the short arm of chromosome 9 of Zea mays, encodes the enzyme sucrose synthase (EC 2.4.1.13). The gene is known to be expressed in the endosperm of the developing maize kernel and seems to be involved in sucrose breakdown prior to starch synthesis. We have analyzed different tissues of the maize plant for transcripts of the Shrunken gene and have found rather high transcription rates in the etiolated shoot and the primary root of the germinating kernel. If the etiolated seedlings are illuminated, the transcript level drops by about 95% in the greening plant parts (1st and 2nd leaves) which are active in photosynthesis. A very low transcript level is found in mature green leaves where sucrose is formed from products of photosynthesis via a separate pathway. Upon anaerobic stress of the young seedling, the level of Shrunken transcripts increases 10 and 20 times in shoot and root tissue respectively. Apparently anaerobic induction supersedes the negative control that is observed after illumination in the 1st and 2nd leaves. From the experiments outlined here we conclude that the anaerobic protein 87 (ANP87, Hake et al. 1985) is encoded by the Shrunken locus. While the expression of the Shrunken gene varies in different tissues and in response to external stimuli, transcription of the second sucrose synthase (B) gene seems to be irresponsive to anaerobic stress and to be expressed at a similar low level in all of the tissues examined.


Subject(s)
Gene Expression Regulation , Glucosyltransferases/genetics , Transcription, Genetic , Zea mays/genetics , Alcohol Dehydrogenase/genetics , Anaerobiosis , Chromosomes , Genes , Nucleic Acid Hybridization , Plant Proteins/genetics , Poly A , RNA , RNA, Messenger , Zea mays/enzymology
SELECTION OF CITATIONS
SEARCH DETAIL