To Bag or Not to Bag? How AudioMoth-Based Passive Acoustic Monitoring Is Impacted by Protective Coverings.

Bare board AudioMoth recorders offer a low-cost, open-source solution to passive acoustic monitoring (PAM) but need protecting in an enclosure. We were concerned that the choice of enclosure may alter the spectral characteristics of recordings. We focus on polythene bags as the simplest enclosure and assess how their use affects acoustic metrics. Using an anechoic chamber, a series of pure sinusoidal tones from 100 Hz to 20 kHz were recorded on 10 AudioMoth devices and a calibrated Class 1 sound level meter. The recordings were made on bare board AudioMoth devices, as well as after covering them with different bags. Linear phase finite impulse response filters were designed to replicate the frequency response functions between the incident pressure wave and the recorded signals. We applied these filters to ~1000 sound recordings to assess the effects of the AudioMoth and the bags on 19 acoustic metrics. While bare board AudioMoth showed very consistent spectral responses with accentuation in the higher frequencies, bag enclosures led to significant and erratic attenuation inconsistent between frequencies. Few acoustic metrics were insensitive to this uncertainty, rendering index comparisons unreliable. Biases due to enclosures on PAM devices may need to be considered when choosing appropriate acoustic indices for ecological studies. Archived recordings without adequate metadata may potentially produce biased acoustic index values and should be treated cautiously.

Methods of acoustic gas flux inversion-Investigation into the initial amplitude of bubble excitation.

Passive acoustic inversion techniques for measuring gas flux into the water column have the potential to be a powerful tool for the long-term monitoring and quantification of natural marine seeps and anthropogenic emissions. Prior inversion techniques have had limited precision due to lack of constraints on the initial amplitude of a bubble's excitation following its release into the water column ( R). R is determined by observing the acoustic signal of bubbles released from sediment in a controlled experiment and its use is demonstrated by quantifying the flux from a volcanic CO2 seep offshore Panarea (Italy), improving the precision by 78%.

Measurement of bistatic sea surface scattering with a parametric acoustic source.

This work presents the results from a series of bistatic sea surface scattering experiments conducted in shallow water using a parametric acoustic array as a source and a receiver comprising a horizontal linear array. The experiments measured scattering at three frequencies (4, 8, and 15 kHz) and at three incident grazing angles (13º, 20º, and 30º). The measurements were made over a 5 day period during which a variety of environmental conditions were encountered. This paper provides an outline of the experiments and presents some results for the forward scattering strength. The results show that the wave direction has a significant effect on the surface forward scattering. At each incident grazing angle, the fluctuations of scattering strength due to environmental conditions decreases as the frequency increases.

The response of common carp (Cyprinus carpio) to insonified bubble curtains.

Acoustic bubble curtains have been marketed as relatively low cost and easily maintained behavioural deterrents for fisheries management. Their energy efficiency can be improved by reducing air flow and exploiting bubble resonance. In a series of three flume experiments, we: (1) investigated the reactions of carp to a low air flow bubble curtain, (2) compared the effectiveness of resonant versus non-resonant insonified bubble curtains (for the same volume flux of gas injected through the nozzles) to deter passage, and determined the stimuli responsible for eliciting deterrence, and (3) included the effect of visual cues generated by the bubble curtain. This study showed that bubble curtains with a higher proportion of resonant bubbles deterred carp relatively better. Passage rejection was likely influenced by multiple cues at distances within a body length of the fish- specifically the rate of change in both particle motion and flow velocity caused by rising bubbles. All acoustic bubble curtains were less effective in the presence of daylight, suggesting that vision plays an important role at mediating carp reactions. We discuss the importance of ascertaining the bubble size distribution, in addition to the gas flow rate and aperture size, when characterising acoustically active bubble curtains.

Acoustic propagation in gassy intertidal marine sediments: An experimental study.

The need to predict acoustic propagation through marine sediments that contain gas bubbles has become increasingly important for civil engineering and climate studies. There are relatively few in situ acoustic wave propagation studies of muddy intertidal sediments, in which bubbles of biogenic gas (generally methane, a potent greenhouse gas) are commonly found. We used a single experimental rig to conduct two in situ intertidal acoustical experiments to improve understanding of acoustic remote sensing of gassy sediments, eventually including gas bubble size distributions. In the first experiment, we measured sediment sound speed and attenuation between four aligned hydrophones for a quasi-plane wave propagating along the array. The second experiment involved a focused insonified sediment volume created by two transducers emitting coincident sound beams at different frequencies that generated bubble-mediated acoustic signals at combination frequencies. The results from sediment core analyses, and comparison of in situ acoustic velocity and attenuation values with those of water-saturated sediments, together provide ample evidence for the presence of in situ gas bubbles in the insonified volumes of sediments. These datasets are suitable for linear and non-linear inversion studies that estimate in situ greenhouse gas bubble populations, needed for future acoustical remote sensing applications.

Collective behaviour of the European minnow (Phoxinus phoxinus) is influenced by signals of differing acoustic complexity.

Collective behaviour, such as shoaling in fish, benefits individuals through a variety of activities such as social information exchange and anti-predator defence. Human driven disturbance (e.g. anthropogenic noise) is known to affect the behaviour and physiology of individual animals, but the disruption of social aggregations of fish remains poorly understood. Anthropogenic noise originates from a variety of activities and differs in acoustic structure, dominant frequencies, and spectral complexity. The response of groups of fish may differ greatly, depending on the type of noise, and how it is perceived (e.g. threatening or attractive). In a controlled laboratory study, high resolution video tracking in combination with fine scale acoustic mapping was used to investigate the response of groups of European minnows (Phoxinus phoxinus) to signals of differing acoustic complexity (sinewave tones vs octave band noise) under low (150 Hz) and high (2200 Hz) frequencies. Fish startled and decreased their mean group swimming speed under all four treatments, with low frequency sinewave tones having the greatest influence on group behaviour. The shoals exhibited spatial avoidance during both low frequency treatments, with more time spent in areas of lower acoustic intensity than expected. This study illustrates how noise can influence the spatial distribution and social dynamics within groups of fish, and owing to the high potential for freshwater aquatic environments to be influenced by anthropogenic activity, wider consequences for populations should be further investigated.

Mobile surveys and machine learning can improve urban noise mapping: Beyond A-weighted measurements of exposure.

Urban noise pollution is a major environmental issue, second only to fine particulate matter in its impacts on physical and mental health. To identify who is affected and where to prioritise actions, noise maps derived from traffic flows and propagation algorithms are widely used. These may not reflect true levels of exposure because they fail to consider noise from all sources and may leave gaps where roads or traffic data are absent. We present an improved approach to overcome these limitations. Using walking surveys, we recorded 52,366 audio clips of 10 s each along 733 km of routes throughout the port city of Southampton. We extracted power levels in low (11 to 177 Hz), mid (177 Hz to 5.68 kHz), high (5.68 to 22.72 kHz) and A-weighted frequencies and then built machine-learning (ML) models to predict noise levels at 30 m resolution across the entire city, driven by urban form. Model performance (r2) ranged from 0.41 (low frequencies) to 0.61 (mid frequencies) with mean absolute errors of 4.05 to 4.75 dB. The main predictors of noise were related to modes of transport (road, air, rail and water) but for low frequencies, port activities were also important. When mapped to the city scale, A-weighted frequencies produced a similar spatial pattern to mid-frequencies, but did not capture the major sources of low frequency noise from the port or scattered hotspots of high frequencies. We question whether A-weighted noise mapping is adequate for health and wellbeing impact assessments. We conclude that mobile surveys combined with ML offer an alternative way to map noise from all sources and at fine resolution across entire cities that may more accurately reflect true exposures. Our approach is suitable for noise data gathered by citizen scientists, or from a network of sensors, as well as from structured surveys.

Automated extraction of dolphin whistles-A sequential Monte Carlo probability hypothesis density approach.

The need for automated methods to detect and extract marine mammal vocalizations from acoustic data has increased in the last few decades due to the increased availability of long-term recording systems. Automated dolphin whistle extraction represents a challenging problem due to the time-varying number of overlapping whistles present in, potentially, noisy recordings. Typical methods utilize image processing techniques or single target tracking, but often result in fragmentation of whistle contours and/or partial whistle detection. This study casts the problem into a more general statistical multi-target tracking framework and uses the probability hypothesis density filter as a practical approximation to the optimal Bayesian multi-target filter. In particular, a particle version, referred to as a sequential Monte Carlo probability hypothesis density (SMC-PHD) filter, is adapted for frequency tracking and specific models are developed for this application. Based on these models, two versions of the SMC-PHD filter are proposed and the performance of these versions is investigated on an extensive real-world dataset of dolphin acoustic recordings. The proposed filters are shown to be efficient tools for automated extraction of whistles, suitable for real-time implementation.

Group behavior and tolerance of Eurasian minnow (Phoxinus phoxinus) in response to tones of differing pulse repetition rate.

Behavioral guidance systems are commonly used in freshwater fish conservation. The biological relevance of sound to fish and recorded responses to human-generated noise supports the viability of the use of acoustics as an effective stimulus in such technologies. Relatively little information exists on the long-term responses and recovery of fish to repeated acoustic exposures. In a controlled laboratory study, the response and tolerance of Eurasian minnow (Phoxinus phoxinus) shoals to tonal signals (150 Hz of 1 s pulse duration) differing only in temporal characteristics ("continuous," "slow," "intermediate," or "fast" pulse repetition rate) were investigated. In comparison to independent control groups, fish increased their mean group swimming speed, decreased inter-individual distance, and became more aligned in response to the onset of all four acoustic treatments. The magnitude of response, and time taken to develop a tolerance to a treatment differed according to pulse repetition rate. Groups were found to have the greatest and longest lasting response to tone sequences tested in this study when they were pulsed at an intermediate rate of 0.2 s-1. This study illustrates the importance of understanding the response of fish to acoustic signals, and will assist toward the development of longer-term effective acoustic guidance systems.

Underwater radiated noise from hydrofoils in coastal water.

Underwater noise from commercial shipping throughout the oceans has been increasing over the past decades and the environmental impact of this noise remains an area of great uncertainty. This has led to the measurement of noise from commercial vessels in order to understand the impacts that these vessels may engender. Hydrofoils are used by ferries in various locations around the world and locally may be a significant contributing factor of the soundscape. However, the investigation on underwater radiated noise from the activity of hydrofoils in the field has not been widely conducted. This article is an attempt to characterize the noise from hydrofoils in the field. Detailed measurements in the coastal water close to the Panarea port, Italy are reported. The investigation describes the broadband frequency spectrum with the main energy approximately centered on 30-130 Hz but covering frequencies up to tens of kHz. A key result was that the spectrum of the noise varied between the three stages (displacement, transition, and foiling) of the hydrofoils heading into or out of the port.

Three-dimensional finite element simulation of acoustic propagation in spiral bubble net of humpback whale.

In 2004, Leighton hypothesized that the acoustic calls emitted by humpback whales when feeding using bubble nets, may enhance the effectiveness of the net in confining prey (such as herring) by forming a "wall of sound" with a quiet zone within. Modelling of the acoustics of this phenomenon was previously restricted to 2D; this paper conducts a 3D model of the propagation of signals resembling those emitted by humpback whales when bubble netting, projected into an upward spiral bubble net which data to date suggest is the accurate form for the bubble net in 3D space. In this study, the feeding calls were analyzed in the time-frequency domain to extract acoustic information sufficient to allow modeling of the resulting spatial distribution of acoustic pressure and particle velocity, and how they vary over the duration of the call. Sound propagation in the bubble net was described by using a linear steady-state formulation for an effective medium of bubbly water. Using the predicted attenuation, phase velocity and density in bubbly water, a 3D finite element model was constructed to numerically simulate the upward-spiral bubble net which consists of a mixture of bubbles that exhibit a range of radii. The acoustic pressure field and particle motion field were both calculated within the bubble net. The simulation results show that the energy of the whale feeding call could be effectively focused in the bubble net, generating intensive sound pressure and particle motion fields in the bubbly arm of the net, but with some "quiet" regions closer to the center of the net, as Leighton hypothesized. Furthermore, when the hearing ability of herring is taken into consideration, the results suggest that this acoustic focusing effect could be a plausible factor in trapping them in the bubble net. It also allows speculation on the possible enhancements that the time-varying nature of the call during feeding could give to the whale in this mechanism for the bubble net feeding by humpback whales.

Measurements of ultrasonic deterrents and an acoustically branded hairdryer: Ambiguities in guideline compliance.

Acoustic radiation from three commercial pest deterrents and two hair dryers were measured in an anechoic chamber. The deterrents were chosen because the frequency range at which they emit the most energy is either in the very high-frequency sound band (11.2-17.8 kHz) or the ultrasound band (greater than 17.8 kHz). These are sources that may be heard by a subset of the general population, with the young typically having better high frequency sensitivity. A hairdryer reported to increase the frequency of the motor noise above the audible hearing range was compared with a standard hairdryer. The outputs of the deterrents are compared against six international regulations and guidelines for audible and ultrasound exposure. Multiple ambiguities in the application of these guidelines are discussed. These ambiguities could lead to a device being considered as in compliance despite unconventionally high levels. Even if a device measured here meets a guideline, actual exposures can exceed those taken here and may therefore breach guidelines if the listener is closer to the device or reflections increase the exposure level.

Public exposure to ultrasound and very high-frequency sound in air.

Recent work showing the presence of a new generation of ultrasound (US) sources in public places has reopened the debate about whether there are adverse effects of US on humans, and has identified weaknesses in standards and exposure guidelines. Systems that rely on very high-frequency sound (VHFS) and US include public-address voice-alarm (PAVA) systems (whose operational status is often monitored using tones at ∼20 kHz) and pest deterrents. In this study, sound pressure levels (SPLs) produced by 16 sources that were either publically available or installed in busy public spaces were measured. These sources were identified through a citizen science project, wherein members of the public were asked to provide smartphone recordings of VHFS/US sources. With measurements made in realistic listening positions, pest deterrents were found that produced levels of up to 100 dB SPL at ∼20 kHz, and a hand dryer was found to produce 84 dB SPL at 40 kHz. PAVA systems were found to emit lower levels of up to 76 dB SPL at ∼20 kHz. Pest deterrents measured breach recommended safe listening limits for public exposure for people who are nearby even for relatively short periods.

Effects of very high-frequency sound and ultrasound on humans. Part II: A double-blind randomized provocation study of inaudible 20-kHz ultrasound.

Some people have reported symptoms such as nausea, dizziness, and headaches that they attribute to ultrasound (US) emitted by devices in public places. The primary aim of the present study was to investigate whether inaudible US can provoke adverse symptoms compared to a sham presentation, under double-blind conditions. A second aim was to investigate whether the expectation of US being present could provoke adverse symptoms (a nocebo response). The US stimulus was a 20 kHz tone presented continuously for 20 min set to at least 15 dB below the participants' detection threshold, giving a typical sound pressure level (SPL) of 84 dB. No evidence that US provoked symptoms was found, but there was evidence of small nocebo effects. A case study on an individual with high self-reported sensitivity to US gave similar results. The present study did not reproduce the severe symptoms reported previously by some members of the public; this may be due to the SPL or duration of the stimulus, or strength of the nocebo stimulus. These findings cannot be used to predict outcomes from exposures to sounds that are audible to the individual in question, or to sounds with higher SPLs, longer durations, or different frequency content.

Effects of very high-frequency sound and ultrasound on humans. Part I: Adverse symptoms after exposure to audible very-high frequency sound.

Various adverse symptoms resulting from exposure to very high-frequency sound (VHFS) and ultrasound (US) have previously been reported. This study aimed to establish whether these symptoms are experienced under controlled laboratory conditions and are specific to VHFS/US. To do this, participants were exposed to VHFS/US (at frequencies between 13.5 and 20 kHz and sound pressure levels between 82 and 92 dB) and to a 1 kHz reference stimulus, both at 25 dB above their hearing threshold. The VHFS/US and reference stimuli were presented 4 times, each time for 3 min, during which participants performed a sustained attention task, rated their symptom severity, and had their galvanic skin response (GSR) measured to assess their level of anxiety. Prior to exposure, participants were assigned either to a symptomatic or an asymptomatic group, based on their prior history of symptoms that they attributed to VHFS/US. In both groups, overall discomfort ratings were higher in the VHFS/US condition than the reference condition. In the symptomatic group only, difficulty concentrating and annoyance were also rated higher in the VHFS/US than the reference condition. No difference between the two stimulus conditions was seen in performance on the attention task or on average GSRs for either group.

Asymmetric transfer of CO2 across a broken sea surface.

Most estimates of the climatically-important transfer of atmospheric gases into, and out of, the ocean assume that the ocean surface is unbroken by breaking waves. However the trapping of bubbles of atmospheric gases in the ocean by breaking waves introduces an asymmetry in this flux. This asymmetry occurs as a bias towards injecting gas into the ocean where it dissolves, and against the evasion/exsolution of previously-dissolved gas coming out of solution from the oceans and eventually reaching the atmosphere. Here we use at-sea measurements and modelling of the bubble clouds beneath the ocean surface to show that the numbers of large bubbles found metres below the sea surface in high winds are sufficient to drive a large and asymmetric flux of carbon dioxide. Our results imply a much larger asymmetry for carbon dioxide than previously proposed. This asymmetry contradicts an assumption inherent in most existing estimates of ocean-atmosphere gas transfer. The geochemical and climate implications include an enhanced invasion of carbon dioxide into the stormy temperate and polar seas.

A blind source separation approach for humpback whale song separation.

Many marine mammal species are highly social and are frequently encountered in groups or aggregations. When conducting passive acoustic monitoring in such circumstances, recordings commonly contain vocalizations of multiple individuals which overlap in time and frequency. This paper considers the use of blind source separation as a method for processing these recordings to separate the calls of individuals. The example problem considered here is that of the songs of humpback whales. The high levels of noise and long impulse responses can make source separation in underwater contexts a challenging proposition. The approach present here is based on time-frequency masking, allied to a noise reduction process. The technique is assessed using simulated and measured data sets, and the results demonstrate the effectiveness of the method for separating humpback whale songs.

Acoustic wave propagation in gassy porous marine sediments: The rheological and the elastic effects.

The preceding paper in this series [Mantouka, Dogan, White, and Leighton, J. Acoust. Soc. Am. 140, 274-282 (2016)] presented a nonlinear model for acoustic propagation in gassy marine sediments, the baseline for which was established by Leighton [Geo. Res. Lett. 34, L17607 (2007)]. The current paper aims further advancement on those two studies by demonstrating the particular effects of the sediment rheology, the dispersion and dissipation of the first compressional wave, and the higher order re-scattering from other bubbles. Sediment rheology is included through the sediment porosity and the definition of the contact interfaces of bubbles with the solid grains and the pore water. The intrinsic attenuation and the dispersion of the compressional wave are incorporated using the effective fluid density model [Williams, J. Acoust. Soc. Am. 110, 2276-2281 (2001)] for the far field (fully water-saturated sediment). The multiple scattering from other bubbles is included using the method of Kargl [J. Acoust. Soc. Am. 11, 168-173 (2002)]. The overall nonlinear formulation is then reduced to the linear limit in order to compare with the linear theory of Anderson and Hampton [J. Acoust. Soc. Am. 67, 1890-1903 (1980)], and the results for the damping coefficients, the sound speed, and the attenuation are presented.

Automated tracking of dolphin whistles using Gaussian mixture probability hypothesis density filters.

This work considers automated multi target tracking of odontocete whistle contours. An adaptation of the Gaussian mixture probability hypothesis density (GM-PHD) filter is described and applied to the acoustic recordings from six odontocete species. From the raw data, spectral peaks are first identified and then the GM-PHD filter is used to simultaneously track the whistles' frequency contours. Overall over 9000 whistles are tracked with a precision of 85% and recall of 71.8%. The proposed filter is shown to track whistles precisely (with mean deviation of 104 Hz, about one frequency bin, from the annotated whistle path) and 80% coverage. The filter is computationally efficient, suitable for real-time implementation, and is widely applicable to different odontocete species.

Does Masking Matter? Shipping Noise and Fish Vocalizations.

Shipping creates large near-field background noises at levels similar to or higher than fish vocalizations and in the same critical bandwidths. This noise has the potential to "mask" biologically important signals and prevent fish from hearing them; any interference with the detection and recognition of sounds may impact fish survival. The Lombard effect, whereby vocalizations are altered to reduce or exclude masking effects, is an adaptation that has been observed in mammals and birds. Research is needed to establish whether the Lombard effect occurs in fish to gain a better understanding of the implications of noise pollution on fish populations.