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2.
Arthritis Rheumatol ; 72(8): 1330-1340, 2020 08.
Article in English | MEDLINE | ID: mdl-32182401

ABSTRACT

OBJECTIVE: Patients with SjÓ§gren's syndrome (SS) have an increased risk of developing malignant B cell lymphomas, particularly mucosa-associated lymphoid tissue (MALT)-type lymphomas. We have previously shown that a predominant proportion of patients with SS-associated salivary gland MALT lymphoma express somatically hypermutated IgM with strong amino acid sequence homology with stereotypic rheumatoid factors (RFs). The present study was undertaken in a larger cohort of patients with SS-associated MALT lymphoma to more firmly assess the frequency of RF reactivity and the significance of somatic IGV-region mutations for RF reactivity. METHODS: B cell antigen receptors (BCRs) of 16 patients with SS-associated salivary gland MALT lymphoma were analyzed. Soluble recombinant IgM was produced of 12 MALT lymphoma samples, including 1 MALT lymphoma sample that expressed an IgM antibody fitting in a novel IGHV3-30-encoded stereotypic IGHV subset. For 4 of the 12 IgM antibodies from MALT lymphoma samples, the somatically mutated IGHV and IGKV gene sequences were reverted to germline configurations. Their RF activity and binding affinity were determined by enzyme-linked immunosorbent assay and surface plasmon resonance, respectively. RESULTS: Nine (75%) of the 12 IgM antibodies identified in patients with SS-associated salivary gland MALT lymphoma displayed strong monoreactive RF activity. Reversion of the IGHV and IGKV mutations to germline configuration resulted in RF affinities for IgG that were significantly lower for 3 of the 4 somatically mutated IgM antibodies. In stereotypic IGHV3-7/IGKV3-15-encoded RFs, a recurrent replacement mutation in the IGKV3-15-third complementarity-determining region was found to play a pivotal role in the affinity for IgG-Fc. CONCLUSION: A majority of patients with SS-associated salivary gland MALT lymphoma express somatically mutated BCRs that are selected for monoreactive, high-affinity binding of IgG-Fc. These data underscore the notion that soluble IgG, most likely in immune complexes in inflamed tissues, is the principal autoantigen in the pathogenesis of a variety of B cell lymphomas, particularly SS-associated MALT lymphomas.


Subject(s)
Immunoglobulin G/immunology , Lymphoma, B-Cell, Marginal Zone/genetics , Mutation/immunology , Rheumatoid Factor/immunology , Sjogren's Syndrome/genetics , Humans , Lymphoma, B-Cell, Marginal Zone/immunology , Receptors, Antigen, B-Cell/genetics , Receptors, Antigen, B-Cell/immunology , Salivary Glands/immunology
3.
Am J Pathol ; 186(12): 3273-3284, 2016 12.
Article in English | MEDLINE | ID: mdl-27750045

ABSTRACT

Follicular lymphoma (FL) is an indolent B-cell non-Hodgkin lymphoma able to transform into germinal center-type diffuse large B-cell lymphoma. We describe four extraordinary cases of FL, which progressed to TdT+CD20- precursor B-lymphoblastic lymphoma (B-LBL). Fluorescence in situ hybridization analysis showed that all four B-LBLs had acquired a MYC translocation on transformation. Comparative genomic hybridization analysis of one case demonstrated that in addition to 26 numerical aberrations that were shared between the FL and B-LBL, deletion of CDKN2A/B and 17q11, 14q32 amplification, and copy-neutral loss of heterozygosity of 9p were gained in the B-LBL cells. Whole-exome sequencing revealed mutations in FMN2, NEB, and SYNE1 and a nonsense mutation in KMT2D, all shared by the FL and B-LBL, and TNFRSF14, SMARCA2, CCND3 mutations uniquely present in the B-LBL. Remarkably, all four FL-B-LBL pairs expressed IgG. In two B-LBLs, evidence was obtained for ongoing rearrangement of IG light chain variable genes and expression of the surrogate light chain. IGHV mutation analysis showed that all FL-B-LBL pairs harbored identical or near-identical somatic mutations. From the somatic gene alterations found in the IG and non-IG genes, we conclude that the FLs and B-LBLs did not develop in parallel from early t(14;18)-positive IG-unmutated precursors, but that the B-LBLs developed from preexistent FL subclones that accumulated additional genetic damage.


Subject(s)
Immunoglobulin Light Chains, Surrogate/genetics , Immunoglobulin gamma-Chains/genetics , Lymphoma, B-Cell/genetics , Lymphoma, Follicular/genetics , B-Lymphocytes/pathology , Comparative Genomic Hybridization , Cyclin D3/genetics , Cyclin-Dependent Kinase Inhibitor p15/genetics , Cyclin-Dependent Kinase Inhibitor p16 , Cyclin-Dependent Kinase Inhibitor p18/genetics , DNA Mutational Analysis , Female , Germinal Center/pathology , Humans , Immunoglobulin Light Chains, Surrogate/metabolism , Immunoglobulin gamma-Chains/metabolism , In Situ Hybridization, Fluorescence , Lymphoma, B-Cell/pathology , Lymphoma, Follicular/pathology , Male , Middle Aged , Mutation , Neurofibromin 1/genetics , Receptors, Tumor Necrosis Factor, Member 14/genetics , Transcription Factors/genetics , Translocation, Genetic , Young Adult
5.
Arthritis Rheumatol ; 67(4): 1074-83, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25546553

ABSTRACT

OBJECTIVE: Among autoimmune diseases, Sjögren's syndrome (SS) is most strongly associated with the development of malignant B cell lymphoma, in particular mucosa-associated lymphoid tissue (MALT)-type lymphoma. Previously, we have shown that in ∼40% of cases of salivary gland MALT lymphoma, high-affinity stereotypic rheumatoid factor (RF) B cell receptors, specific for IgG-Fc, are expressed. This study was undertaken to investigate whether in the inflamed salivary glands of patients with SS, a similar RF-biased Ig repertoire is present. METHODS: Extensive analyses of the B cell Ig VH region repertoire were performed on microdissected tissue samples from the labial salivary glands of 4 patients with SS. RESULTS: All SS labial salivary glands harbored expanded B cell clones, of which 1 or 2 were highly expanded and detected in >50% of the microdissected samples. However, among the identified 464 distinct Ig clonotypes, only 3 stereotypic RF-expressing clones were detected. In 2 patients with SS, an RF-expressing clone was detected at low frequency in 1 of the microdissected samples, whereas 1 patient with SS harbored a highly expanded RF-expressing clone that was detected in all microdissected samples and also detected in the peripheral blood. Two years after analysis of this sample, the latter patient developed a diffuse large B cell lymphoma originating from the same RF clone. CONCLUSION: Inflamed labial salivary glands in patients with SS generally harbor 1 or 2 highly expanded B cell clones. The repertoire strongly biased toward stereotypic RFs in salivary gland MALT lymphomas is not a reflection of a similar repertoire in the inflamed salivary glands of patients with SS; rather, in the latter, the repertoire is based on a strong selection advantage of incidental stereotypic RF-expressing B cells.


Subject(s)
Lymphoid Tissue/metabolism , Lymphoma, B-Cell, Marginal Zone/metabolism , Mucous Membrane/metabolism , Rheumatoid Factor/metabolism , Salivary Glands/metabolism , Sjogren's Syndrome/metabolism , Adolescent , Adult , Aged , B-Lymphocytes/metabolism , B-Lymphocytes/pathology , Child , Female , Humans , Lymphoid Tissue/pathology , Lymphoma, B-Cell, Marginal Zone/pathology , Male , Middle Aged , Mucous Membrane/pathology , Salivary Glands/pathology , Sjogren's Syndrome/pathology , Young Adult
6.
Blood ; 112(8): 3355-61, 2008 Oct 15.
Article in English | MEDLINE | ID: mdl-18687986

ABSTRACT

Extranodal marginal zone B-cell lymphomas (MZBCLs) arise on a background of chronic inflammation resulting from organ-specific autoimmunity, infection, or by unknown causes. Well-known examples are salivary gland MZBCL in Sjögren's sialadenitis and gastric MZBCL in Helicobacter pylori gastritis. MZBCLs express CXCR3, a receptor for interferon-gamma-induced chemokines highly expressed in the chronic inflammatory environment. The immunoglobulin (Ig) variable heavy/light chain (IgV(H)/IgV(L)) gene repertoire of salivary gland and gastric MZBCL appears restricted and frequently encodes B-cell receptors with rheumatoid factor reactivity. Primary cutaneous marginal zone B-cell lymphomas (PCMZLs) are regarded as the skin-involving counterparts of extranodal MZBCLs. Although PCMZLs have been associated with Borrelia burgdorferi dermatitis, PCMZLs generally arise because of unknown causes. We studied an extensive panel of PCMZLs and show that PCMZLs do not conform to the general profile of extranodal MZBCL. Whereas most noncutaneous MZBCLs express IgM, PCMZLs in majority express IgG, IgA, and IgE and do not show an obvious immunoglobulin repertoire bias. Furthermore, the isotype-switched PCMZLs lack CXCR3 and seem to arise in a different inflammatory environment, compared with other extranodal MZBCLs.


Subject(s)
Immunoglobulins/metabolism , Lymphoma, B-Cell, Marginal Zone/immunology , Lymphoma, B-Cell, Marginal Zone/metabolism , Th2 Cells/metabolism , Antigens, CD20/biosynthesis , Complementarity Determining Regions/metabolism , Humans , Immunoglobulin A/chemistry , Immunoglobulin Class Switching , Immunoglobulin E/chemistry , Immunoglobulin G/chemistry , Inflammation , Interferon-gamma/metabolism , Lymphoma, B-Cell, Marginal Zone/pathology , Models, Biological , Receptors, CXCR3/metabolism , Rheumatoid Factor/metabolism
7.
J Exp Med ; 204(11): 2655-65, 2007 Oct 29.
Article in English | MEDLINE | ID: mdl-17938234

ABSTRACT

To reveal migration trails of antigen-responsive B cells in lymphoid tissue, we analyzed immunoglobulin (Ig)M-V(H) and IgG-V(H) transcripts of germinal center (GC) samples microdissected from three reactive human lymph nodes. Single B cell clones were found in multiple GCs, one clone even in as many as 19 GCs. In several GCs, IgM and IgG variants of the same clonal origin were identified. The offspring of individual hypermutated IgG memory clones were traced in multiple GCs, indicating repeated engagement of memory B cells in GC reactions. These findings imply that recurring somatic hypermutation progressively drives the Ig repertoire of memory B cells to higher affinities and infer that transforming genetic hits in non-Ig genes during lymphomagenesis do not have to arise during a single GC passage, but can be collected during successive recall responses.


Subject(s)
B-Lymphocytes/immunology , Immunologic Memory , Lymph Nodes/immunology , Lymphocyte Activation , B-Lymphocytes/cytology , Cell Division , Clone Cells , Gene Amplification , Humans , Immunoglobulin Heavy Chains/genetics , Immunoglobulin M/genetics , Lymph Nodes/cytology , T-Lymphocytes/immunology , Transcription, Genetic
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