ABSTRACT
BACKGROUND & OBJECTIVE: Raddeanin A, a triterpenoid saponin from Anemone raddeana Regel, has good antitumor activity in vitro. This study was to investigate its antitumor effects on tumor cell xenografts in mice. METHODS: The inhibitory effects of raddeanin A on the proliferation of human nasopharyngeal carcinoma KB cells and ovarian cancer SKOV3 cells were measured by MTT assay. The inhibitory effects of raddeanin A injection on the growth of sarcoma S180, liver cancer H22 and cervical carcinoma U14 cell xenografts in mice and the effect of raddeanin A lavage on the growth of S180 cell xenografts were measured. The acute toxicity of raddeanin A was also measured. RESULTS: The 50% inhibition concentration (IC(50)) of raddeanin A was 4.64 microg/mL for KB cells and 1.40 microg/mL for SKOV3 cells. When injected with raddeanin A at a dose of 4.5 mg/kg, the growth inhibition rates of S180, H22 and U14 cell xenografts were 60.5%, 36.2% and 61.8%, respectively. When lavaged with raddeanin A at a dose of 200 mg/kg, the growth inhibition rate of S180 cell xenografts was 64.7%. The median lethal dose (LD50) of raddeanin A lavage was 1.1 g/kg and that of raddeanin A injection was 16.1 mg/kg. CONCLUSION: Raddeanin A has good antitumor activity both in vitro and in vivo, and would be a potential antitumor medicine.
Subject(s)
Anemone/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Drugs, Chinese Herbal/pharmacology , Liver Neoplasms, Experimental/pathology , Sarcoma 180/pathology , Animals , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/toxicity , Cell Line, Tumor , Cell Proliferation/drug effects , Drugs, Chinese Herbal/isolation & purification , Drugs, Chinese Herbal/toxicity , Female , Humans , Male , Mice , Mice, Inbred ICR , Nasopharyngeal Neoplasms/pathology , Neoplasm Transplantation , Ovarian Neoplasms/pathology , Plants, Medicinal/chemistry , Random Allocation , Tumor Burden/drug effects , Uterine Cervical Neoplasms/pathology , Xenograft Model Antitumor AssaysABSTRACT
Besides a series of known sterols and triterpenoids, a new resorcinol (1) and a known resorcinol (2) have been isolated from ethanol extract of Ardisia maculosa for the first time. The structures of these resorcinol derivatives were elucidated as 2-methyl-5-(Z-heptadec-8-enyl) resorcinol and 5-Z-heptadec-8-enyl) resorcinol by HRESI-MS, NMR ((1)H, (13)C, HSQC, HMBC) experiments. In our in vitro assay, compounds 1 and 2 showed no antimicrobial activities, however, compound 2 exhibited cytotoxity activity against human cancer cell line with GI(50) value of 2.14 x 10(- 4) mmol/ml.
Subject(s)
Ardisia/chemistry , Primulaceae/chemistry , Resorcinols/isolation & purification , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Resorcinols/chemistry , Resorcinols/pharmacology , Spectrometry, Mass, Electrospray IonizationABSTRACT
A new aporphine alkaloid, 1-hydroxy-2,3-dimethoxy-6-formyl-6a,7-dehydroaporphine, along with 15 known compounds have been isolated from the stems and leaves of Sabia yunnanensis. Their structures were established on the basis of spectral analysis.
Subject(s)
Aporphines/isolation & purification , Plants, Medicinal/chemistry , Aporphines/chemistry , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Plant Leaves/chemistry , Plant Stems/chemistry , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Infrared , Spectrophotometry, UltravioletABSTRACT
A new amide, 4-hydroxy-N-[4-[3-(4-hydroxy-phenyl)-E-acryloylamino]-butyl]-benzamide, trivially named as glochidiatusamide (1), along with 5 known compounds, beta-sitosterol, daucosterol, coffee acid, ethyl caffeate, and kaempferol-3-O-beta-D-glucoside, were isolated from the ethanol extract of Thyrocarpus glochidiatus Maxim. Their structures were elucidated by the spectral and chemical evidence.
Subject(s)
Benzamides/chemistry , Boraginaceae/chemistry , Benzamides/isolation & purification , China , Chromatography , Ethanol , Molecular Structure , Plant Extracts/chemistry , SpectrophotometryABSTRACT
A novel flavonoid, [2-(3,4-dihydroxy-phenyl)-3,5,7-trihydroxy-4-oxo-4H-chromen-8-yl]-[8-hydroxy-7-(E-4-hydroxy-3-methyl-but-2-enyl)-2,2-dimethyl-chroman-5-yl]-acetic acid methyl ester (10), trivially named sabian, along with 11 known compounds, have been isolated from the stems and leaves of Sabiayunnanensis. Their structures were established on the basis of spectral analysis.
Subject(s)
Flavonoids/isolation & purification , Plants, Medicinal/chemistry , Flavonoids/chemistry , Molecular Structure , Spectrum Analysis/methodsABSTRACT
A new flavone, wightianin (1), along with five known compounds, n-triacontanol (2), betulinic acid (3), oleanolic acid (4), 3,4-O-isopropylidene-shikimic acid (5), and isoquercitrin (6), were isolated from the whole plants of Hypericum wightianum Wall ex Wight et Arn. Their structures were elucidated on the basis of spectral data, including 2D NMR techniques.
Subject(s)
Flavones/chemistry , Hypericum/chemistry , Flavones/isolation & purification , Molecular StructureABSTRACT
A phytochemical study on an ethanol extract of Ardisia arborescens resulted in the isolation of five new diarylundecanones, named ardisinones A-E (1-5). The structures were established by HRESIMS and NMR ((1)H, (13)C, DEPT, HSQC, HMBC) as 11-(2-acetoxy-6-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)undecan-1-one (1), 11-(2-acetoxy-6-hydroxyphenyl)-1-(2,6-dihydroxy-4-methoxyphenyl)undecan-1-one (2), 1-(2,6-dihydroxy-4-methoxyphenyl)-11-(2,6-dihydroxyphenyl)undecan-1-one (3), 1-(2,4,6-trihydroxyphenyl)-11-(2,6-dihydroxyphenyl)undecan-1-one (4), and 1-(2,4,6-trihydroxyphenyl)-11-(2-hydroxyphenyl)undecan-1-one (5). In our in vitro disk diffusion assay, compounds 1 and 4 showed some slight inhibition of three bacteria, while 2 and 3 did not show antimicrobial activity.