ABSTRACT
The effect and mechanism of Huangqin Qingre Chubi Capsules(HQC) on rheumatoid arthritis(RA) were studied.Seventy male SPF rats were randomly divided into normal group, model group, low-(0. 18 g·kg~(-1)), middle-(0. 36 g·kg~(-1)), and high-(0. 72 g·kg~(-1)) dose groups of HQC, methotrexate group(MTX, 0. 75 mg·kg~(-1)), and negative control group(NC group, model +saline). Adjuvant arthritis fibroblast-like synoviocytes(AA-FLS) were divided into normal group, model group, low-, middle-, and high-dose groups of HQC, and negative control group. RT-qPCR and Western blot were used to detect the m RNA and protein expressions of METTL3, SFRP4, ß-catenin, CCND1, c-Myc, MMP3, and fibronectin. The protein expression of MMP3 and ß-catenin was detected by immunofluorescence. The gene expression level of METTL3 on AA-FLS was knocked down to further examine the expression of each gene. ELISA measured the levels of IL-1ß, IL-6, and IL-8. The results showed that compared with the normal group, rats in the model group found redness and swelling in their limbs and significantly increased joint swelling. Compared with the model group, the joint swelling degree of each treatment group significantly decreased(P<0. 05). The paw retraction threshold and body weight mass index both significantly increased(P<0. 05). METTL3 was highly expressed on AA and negatively correlated with the expression of SFRP4. After treatment, the m RNA and protein expression of METTL3, ß-catenin, CCND1, c-Myc, fibronectin, and MMP3 were significantly decreased on AA-FLS(P< 0. 05). Compared with the model group, knocking down METTL3 resulted in reduced m RNA and protein expression of ß-catenin, CCND1, c-Myc, fibronectin, and MMP3(P< 0. 05). At the same time, the m RNA and protein expressions of ß-catenin, CCND1, c-Myc, fibronectin, and MMP3 in the HQC+METTL3 knockdown group were significantly lower than those in the METTL3 knockdown group(P<0. 05). HQC could reduce the levels of IL-1ß, IL-6, and IL-8 to varying degrees(P<0. 05). The results indicate that HQC has a significant improvement effect on arthritis in AA rats. The expression of METTL3 is significantly increased in synovial tissue and AA-FLS of AA rats, which may be a potential target for the diagnosis and treatment of RA. HQC improves RA through the METTL3-SFRP4/Wnt/ß-catenin signaling pathway and has significant antiinflammatory and anti-rheumatic effects.
Subject(s)
Arthritis, Rheumatoid , Capsules , Drugs, Chinese Herbal , Wnt Signaling Pathway , beta Catenin , Animals , Arthritis, Rheumatoid/drug therapy , Arthritis, Rheumatoid/metabolism , Arthritis, Rheumatoid/genetics , Drugs, Chinese Herbal/administration & dosage , Drugs, Chinese Herbal/pharmacology , Rats , Male , Wnt Signaling Pathway/drug effects , beta Catenin/metabolism , beta Catenin/genetics , Methyltransferases/genetics , Methyltransferases/metabolism , Humans , Rats, Sprague-Dawley , Synoviocytes/drug effects , Synoviocytes/metabolism , Proto-Oncogene ProteinsABSTRACT
The correlated spinful Haldane model exhibits rich topological phases consisting of chiral topological superfluids (TSFs) and topological spin density waves. However, most of previous studies mainly focus on the case with the fixed hopping phase or at zero temperature. In this paper, we study the attractive spinful Haldane model with arbitrary phase at finite temperature. The chiral TSFs with Chern numberC = 2 and 4 emerge driven by the phase and temperature. In particular, the temperature can drive aC = 2 topological superfluid from a trivial normal insulator phase at an appropriate interaction. The bulk topology of all TSFs is uncovered by the Wilson loop method, and confirmed by the responses of edge dislocations.
ABSTRACT
The limited excitation efficiency of quantum dots in the detection of subsurface defects in optical elements by quantum dot fluorescence gives rise to insufficient accuracy. To enhance the excitation efficiency of quantum dots, we studied the modulation of the polarization direction of linearly polarized incident light on quantum dot fluorescence. We first apply density matrix evolution theory to study the quantum dots interacting with linearly polarized incident light and emitting fluorescence. The fluorescence intensity exhibits cosine oscillations versus modulated laser polarization. It reaches a maximum value at the polarization angle zero, and then decreases as the angle becomes larger until π/2. The experimental results for the quantum dot in both solutions and subsurface defect of optical elements confirmed these results. For optical elements tagged with CdSe/ZnS quantum dots, the fluorescence intensity increases by 61.7%, and the area for the detected subsurface defects increases by 142.9%. Similarly, for C and InP/ZnS quantum dots, there are also increases in both the fluorescence intensity and the area of subsurface defects. Our study suggests that the subsurface defect detection in optical elements by the linearly polarized incident light could enhance the detection accuracy of subsurface defects in optical elements, and potentially achieve super-resolution imaging of subsurface defects.
ABSTRACT
A Gram-positive, acid-fast, aerobic, rapidly growing and non-motile strain was isolated from lead-zinc mine tailing sampled in Lanping, Yunnan province, Southwest China. 16S rRNA gene sequence analysis showed that the most closely related species of strain KC 300T was Mycolicibacterium litorale CGMCC 4.5724T (98.47â%). Additionally, phylogenomic and specific conserved signature indel analysis revealed that strain KC 300T should be a member of genus Mycolicibacterium, and Mycobacterium palauense CECT 8779T and Mycobacterium grossiae DSM 104744T should also members of genus Mycolicibacterium. The genome size of strain KC 300T was 6.2 Mb with an in silico DNA G+C content of 69.2 mol%. Chemotaxonomic characteristics of strain KC 300T were also consistent with the genus Mycolicibacterium. The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values, as well as phenotypic, physiological and biochemical characteristics, support that strain KC 300T represents a new species within the genus Mycolicibacterium, for which the name Mycolicibacterium arseniciresistens sp. nov. is proposed, with the type strain KC 300T (=CGMCC 1.19494T=JCM 35915T). In addition, we reclassified Mycobacterium palauense and Mycobacterium grossiae as Mycolicibacterium palauense comb. nov. and Mycolicibacterium grossiae comb. nov., respectively.
Subject(s)
Mycobacterium , Zinc , RNA, Ribosomal, 16S/genetics , Base Composition , China , Phylogeny , Sequence Analysis, DNA , DNA, Bacterial/genetics , Bacterial Typing Techniques , Fatty Acids/chemistry , Mycobacterium/geneticsABSTRACT
Strain KC 927T was isolated during an investigation of the soil bacteria diversity on Jiaozi Mountain, central Yunnan, Southwest China. The strain was Gram-stain-negative, rod-shaped, non-motile, oxidase-negative, catalase-positive and aerobic. Results of 16S rRNA gene alignment and phylogenetic analysis indicated that strain KC 927T was a member of the genus Chryseobacterium and closely related to Chryseobacterium caseinilyticum GCR10T (98.4%), Chryseobacterium piscicola DSM 21068T (98.3â%) and 'Chryseobacterium formosus' CCTCC AB 2015118T (97.9â%). With a genome size of 4 348 708 bp, strain KC 927T had 33.5âmol% DNA G+C content and contained 4012 protein-coding genes and 77 RNA genes. The average nucleotide identity and digital DNA-DNA hybridization values between strain KC 927T and C. caseinilyticum GCR10T, C. piscicola DSM 21068T and 'C. formosus' CCTCC AB 2015118T were 80.1, 79.6 and 90.7â%, and 25.5, 23.6 and 42.0â%, respectively. The main polar lipid of strain KC 927T was phosphatidylethanolamine and the respiratory quinone was MK-6. The major fatty acids (≥10â%) were iso-C15â:â0, iso-C17â:â1 ω9c and iso-C17â:â0 3-OH. Evidence from phenotypic, phylogenetic and chemotaxonomic analyses support that strain KC 927T represents a new species of the genus Chryseobacterium, for which the name Chryseobacterium luquanense sp. nov. is proposed. The type strain is KC 927T (=CGMCC 1.18760T=JCM 35707T).
Subject(s)
Caseins , Chryseobacterium , Base Composition , China , Chryseobacterium/genetics , Fatty Acids/chemistry , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , DNA, Bacterial/genetics , Bacterial Typing Techniques , BacteriaABSTRACT
Coherent perfect absorption (CPA) or reflection (CPR) are methods to realize the extreme manipulation on an optical field. We propose a scheme to operate a bistable switch with convertible CPA and/or CPR. Generally, CPA and CPR occur with different input-field phases. For example, CPA is realized when two input probe beams are in phase; instead, CPR is achieved when they are out of phase. In this scheme, a CPA state can be converted to a CPR state by an incoherent field although two input fields are in phase. When we use the incoherent field as a switching field, the CPA (CPR) state is treated as the closed (open) state. As a result, the switching efficiency can theoretically reach a maximum value, i.e., η = 1. In addition, the switch can be operated in the linear regime with a weak input field, and in the nonlinear or bistable regime with a strong input field. Moreover, the efficiency of the bistable switch is sensitively dependent on the input-field intensity. It provides a potential application of this work on sensitive optical detecting.
ABSTRACT
BACKGROUND: Familial hypercholesterolemia (FH) leads to high plasma low-density lipoprotein cholesterol (LDL-C) levels and early cardiovascular morbidity and mortality. We treated a pair of siblings with FH. The cardiovascular manifestations in the proband were more severe than those in his elder sister, although they had almost similar LDL-C levels, ages, and lifestyles. Herein, we report the cases of this family to explore the possible causes of clinical phenotypic differences within the same genetic background. CASE PRESENTATION: We treated a 27-year-old male patient and his 30-year-old sister, both with FH. The coronary angiogram in the male patient revealed 80, 70, and 100% stenosis of the initial, distal right coronary artery branch, and left anterior descending branch, respectively, whereas his sister had almost no coronary stenosis. We treated them accordingly and performed family screening. We found that the LDL-C/particle discordance of the proband is much greater than that of his elder sister. In addition, the average size of LDL-C particle in the proband was smaller than that in his sister. CONCLUSIONS: Patients with FH have a much higher risk of premature atherosclerotic cardiovascular disease, but the clinical manifestations are heterogeneous. The smaller LDL particle size may be the underlying cause for different clinical outcomes in this pair of FH cases and be a potential novel indicator for predicting the prognosis of FH.
Subject(s)
Hyperlipoproteinemia Type II , Siblings , Male , Humans , Cholesterol, LDL , Constriction, Pathologic , PhenotypeABSTRACT
A novel actinobacterium, designated KC 17012T, was isolated from lead zinc tailings collected from Lanping, Yunnan, PR China. Comparative 16S rRNA gene sequencing showed that KC 17012T belonged to the genus Streptomyces and was most closely related to the type strains of Streptomyces neyagawaensis (98.34%), Streptomyces panaciradicis (98.34%) and Streptomyces heilongjiangensis (98.27%). Phylogenetic tree analysis revealed strain KC 17012T formed a distinct clade. The genome size was 8.64 Mbp with a DNA G+C content of 70.8%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain KC 17012T and those of S. neyagawaensis JCM 4796T (25.3 and 81.5â%) and S. panaciradicis NBRC 109811T (30.1 and 85.7â%) were below the thresholds of 70 and 96% for prokaryotic conspecific assignation. The strain formed long straight aerial hyphae which generated regular short rod spores with spiny surfaces. Growth occurred at 10-45 °C, pH 6-8 and with 0-9â% NaCl (w/v). Strain KC 17012T contained ll-diaminopimelic acid and the major whole-cell hydrolysates included glucose, mannose and ribose. The menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). The major cellular fatty acids were iso-C15â:â0, anteiso-C15â:â0, iso-C16â:â0 and anteiso-C17â:â0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified lipid and one unidentified phospholipid. On the basis of the results of a polyphasic taxonomic study, it is concluded that KC 17012T represents a novel species of the genus Streptomyces, for which the name Streptomyces plumbidurans sp. nov., is proposed. The type strain is KC 17012T (CGMCC 4.7704T=JCM 35204T).
Subject(s)
Actinobacteria , Streptomyces , Bacterial Typing Techniques , Base Composition , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Lead , Phospholipids , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2ABSTRACT
An isolate of Gram-stain-negative and strictly aerobic bacterium, designated KC 17139T, was isolated from Jiaozi Mountain sample in Yunnan, China. Cells were non-motile cocci to oval, catalase-positive and oxidase-positive. Growth occurred at 0-7% NaCl (w/v; optimum, 0%), pH 6.0-8.0 (optimum, pH 7.0) and 15-45 °C (optimum, 28-37 °C). The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and four unidentified aminolipids (UALs). Strain KC 17139T contained summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), C18:1 2OH and C16:0 as major cellular fatty acids (> 5%) and ubiquinone-10 as the sole isoprenoid quinone. The 16S rRNA gene sequence analysis indicated that strain KC 17139T shared highest similarities with Siccirubricoccus phaeus 1-3T (96.7%) and Siccirubricoccus deserti SYSU D8009T (95.0%). Strain KC 17139T clustered with the two Siccirubricoccus type strains, but formed a separate branch in both 16S rRNA gene and genome-scale phylogenetic dendrograms. The genomic DNA G + C content of strain KC 17139T was 71.2%. Genomic comparisons between strain KC 17139T and its close relatives showed the highest digital DNA-DNA hybridisation to S. phaeus (35.5%), highest average nucleotide identity to S. phaeus (88.2%), indicating that strain KC 17139T represents a novel species. On the basis of results of phenotypic, chemotaxonomic and molecular analysis, we report a new bacterium strain KC 17139T belonged to genus Siccirubricoccus, for which the name Siccirubricoccus soli sp. nov. is proposed. The type strain is KC 17139T (= CGMCC 1.18756T = JCM 35132T).
Subject(s)
Phosphatidylethanolamines , Ubiquinone , Bacterial Typing Techniques , Cardiolipins , Catalase , China , DNA, Bacterial/genetics , Fatty Acids/chemistry , Nucleotides , Phosphatidylcholines , Phospholipids/chemistry , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sodium Chloride , Soil , Terpenes , Ubiquinone/chemistryABSTRACT
Higher-order topological superconductors and superfluids (SFs) host lower-dimensional Majorana corner and hinge states since novel topology exhibitions on boundaries. While such topological nontrivial phases have been explored extensively, more possible schemes are necessary for engineering Majorana states. In this paper we propose Majorana corner states could be realized in a two-dimensional attractive quantum spin-Hall insulator with opposite in-plane Zeeman energy at two sublattice sites. The appropriate Zeeman field leads to the opposite Dirac mass for adjacent edges of a square sample, and naturally induce Majorana corner states. This topological phase can be characterized by Majorana edge polarizations, and it is robust against perturbations on random potentials and random phase fluctuations as long as the edge gap remains open. Our work provides a new possibility to realize a second-order topological SF in two dimensions and engineer Majorana corner states.
ABSTRACT
Using mean field method and random phase approximation, we studied the phase driven topological exotic states in correlated Haldane model on a honeycomb lattice. It is found that topological spin density waves emerge with the phase change of next-nearest-neighbor hopping. We also investigated the topological properties of these spin density waves, including Chern number, edge state and Hall conductivity. Our work provides a new insight for topological phase transitions in correlated quantum anomalous Hall insulators.
ABSTRACT
We investigate the interacting spinful Haldane model with on-site and nearest-neighbor repulsions on honeycomb lattices. Interactions drive spin-density and charge-density waves competing with each other. This competition enriches the phase diagram of the system greatly, where both topological and non-topological coexistence phases emerge with spin-density and charge-density wave orders. Moreover, the topological phase transition accompanied by the energy gap closure in coexistence phases is of third order. Finally, we study the topological properties consisting ofChernnumbers and edge states for different phases.
ABSTRACT
Second-order topological superconductors host Majorana corner and hinge modes in contrast to conventional edge and surface modes in two and three dimensions. However, the realization of such second-order corner modes usually demands unconventional superconducting pairing or complicated junctions or layered structures. Here we show that Majorana corner modes could be realized using a 2D quantum spin Hall insulator in proximity contact with an s-wave superconductor and subject to an in-plane Zeeman field. Beyond a critical value, the in-plane Zeeman field induces opposite effective Dirac masses between adjacent boundaries, leading to one Majorana mode at each corner. A similar paradigm also applies to 3D topological insulators with the emergence of Majorana hinge states. Avoiding complex superconductor pairing and material structure, our scheme provides an experimentally realistic platform for implementing Majorana corner and hinge states.
ABSTRACT
In sharp contrast to conventional topological superfluids, higher order (order r > 1) topological superfluids in n dimensions do not host n - 1 dimensional Majorana boundary states, instead host n - r dimensional Majorana excitations. In this paper, we propose Majorana corner modes can emerge in a second order superfluid with s-wave pairing, instead of unconventional pairings such as d-wave and [Formula: see text]-wave pairings in most of previous proposals. There are three key ingredients in this scheme consisting of a topological insulator, an in-plane Zeeman field, and an s-wave pairing. Based on the low energy theory for edge states, where the effective Dirac mass sign changes at the corner, we unveil the emergence of Majorana corner modes. Our proposal provides a promising platform for implementing 2D second order topological superfluids and Majorana corner modes.
ABSTRACT
In this paper, we present a study on the repulsive Haldane-Hubbard model with spin-rotation symmetry in square lattices by deriving non-linear σ model for magnetic states. It is found that a chiral spin liquid state as the ground state of the correlated system exists in the [Formula: see text] topological spin-density wave proposed by Wu et al (2016 J. Phys.: Condens. Matter 28 115602), of which the low energy physics can be determined by the Chern-Simons-Hopf gauge field theory.
ABSTRACT
In this paper, based on mean-field approach and random-phase-approximation, we study the magnetic properties of the repulsive Haldane-Hubbard model on a square lattice. We find antiferromagnetic order driven topological spin density waves beyond Landau's symmetry-breaking paradigm, for which the effective low energy physics is determined by Chern-Simons-Hopf gauge field theories with different K matrices.
ABSTRACT
Aquaporins are thought to be associated with water transport and play important roles in cotton (Gossypium hirsutum) fibre elongation. Among aquaporins, plasma membrane intrinsic proteins (PIPs) constitute a plasma-membrane-specific subfamily and are further subdivided into PIP1 and PIP2 groups. In this study, four fibre-preferential GhPIP2 genes were functionally characterized. The selective interactions among GhPIP2s and their interaction proteins were studied in detail to elucidate the molecular mechanism of cotton fibre development. GhPIP2;3 interacted with GhPIP2;4 and GhPIP2;6, but GhPIP2;6 did not interact with GhPIP2;4. Coexpression of GhPIP2;3/2;4 or GhPIP2;3/2;6 resulted in a positive cooperative effect which increased the permeability coefficient of oocytes, while GhPIP2;4/2;6 did not. GhBCP2 (a blue copper-binding protein) inhibited GhPIP2;6 water channel activity through their interaction. Overexpression of GhPIP2 genes in yeast induced longitudinal growth of the host cells. By contrast, knockdown of expression of GhPIP2 genes in cotton by RNA interference markedly hindered fibre elongation. In conclusion, GhPIP2 proteins are the primary aquaporin isoforms in fibres. They selectively form hetero-oligomers in order to regulate their activities to meet the requirements for rapid fibre elongation.
Subject(s)
Aquaporins/metabolism , Cotton Fiber , Gossypium/metabolism , Plant Proteins/metabolism , Animals , Aquaporins/genetics , Cell Membrane/metabolism , Down-Regulation , Fluorescence , Gene Expression Regulation, Plant , Gene Knockdown Techniques , Genes, Plant/genetics , Gossypium/genetics , Oocytes/metabolism , Plant Proteins/genetics , Protein Binding , RNA Interference , Reverse Transcriptase Polymerase Chain Reaction , Schizosaccharomyces/genetics , Subcellular Fractions/metabolism , Two-Hybrid System Techniques , Xenopus laevis/metabolismABSTRACT
Homeodomain-leucine zipper (HD-Zip) proteins are transcription factors unique to plants. In this study, three cDNAs (designated as GhHB2, GhHB3 and GhHB4) encoding HD-Zip proteins were isolated from cotton cDNA library. GhHB2 gene encodes a protein of 300 amino acids, GhHB3 gene encodes a peptide with 254 amino acids, and GhHB4 gene encodes a protein of 281 amino acids. The deduced proteins, which contain the homeodomain and leucine-rich zipper motif, share relatively high similarities with the other plant HD-Zip proteins. Quantitative RT-PCR analysis indicated that GhHB3 and GhHB4 were preferentially expressed in hypocotyls and cotyledons, whereas GhHB2 gene was predominantly expressed in young stems, at relatively high levels in hypocotyls. Expressions of all the three genes were up-regulated in roots, hypocotyls and cotyledons after GA(3) treatments. Additionally, GhHB4 expression was enhanced by 6-BA treatment. A GhHB2 promoter fragment was isolated from cotton by Genome-Walking PCR. Expression of GUS gene controlled under GhHB2 promoter was examined in the transgenic Arabidopsis plants. Strong GUS staining was detected in cotyledon, veins of the emerging leaves and shoot apices of 5- to 15-day-old transgenic seedlings, but GUS activity became more and more weak as the seedlings further developed. In addition, the promoter activity was induced by exogenous GA, indicating that GhHB2 promoter is very active during early seedling development, and may be GA-inducible. The results suggested that the three HB genes may function in early seedling development of cotton and in response to gibberellin signaling.
Subject(s)
Genes, Homeobox , Gossypium/genetics , Homeodomain Proteins/metabolism , Plant Growth Regulators/metabolism , Seedlings/growth & development , Amino Acid Sequence , Arabidopsis/genetics , Arabidopsis/growth & development , Arabidopsis/metabolism , Gene Expression Regulation, Plant , Gene Library , Genes, Plant , Gibberellins/metabolism , Gossypium/growth & development , Gossypium/metabolism , Homeodomain Proteins/genetics , Leucine Zippers , Molecular Sequence Data , Phylogeny , Plants, Genetically Modified/genetics , Plants, Genetically Modified/growth & development , Plants, Genetically Modified/metabolism , Promoter Regions, Genetic , Seedlings/genetics , Sequence AlignmentABSTRACT
Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters water stress such as drought or waterlog during its growth season (Summer). To investigate molecular mechanism of water regulation in cotton plants, three cDNAs encoding the plasma membrane intrinsic protein (PIP) were isolated from cotton root cDNA library, and designated GhPIP1;1, GhPIP2;1 and GhPIP2;2, respectively. All of the three PIP proteins displayed water channel activity in Xenopus laevis oocytes. GhPIP2;1 and GhPIP2;2 proteins, however, showed much higher water transport activity than that of the GhPIP1;1 protein. Northern blot analysis revealed that all of the three genes were preferentially expressed in young roots. Further analysis by Real-time quantitative RT-PCR revealed that the transcripts of all the three genes were accumulated at high levels in 3-day-old young roots, but dramatically declined to much lower levels in 6-14 days old roots during seedling development, suggesting that expressions of the isolated GhPIP genes are developmentally regulated in roots. Additionally, expressions of the three genes were remarkably up-regulated or down-regulated under different stresses such as NaCl, cold, PEG (polyethylene glycol) treatments. Collectively, the results suggest that these genes may be involved in root development and in response to stresses.
Subject(s)
Aquaporins/genetics , Gossypium/genetics , Plant Proteins/genetics , Plant Roots/genetics , Amino Acid Sequence , Aquaporins/chemistry , Aquaporins/metabolism , Blotting, Northern , Cold Temperature , Gene Expression Regulation, Plant/drug effects , Gene Expression Regulation, Plant/genetics , Gossypium/drug effects , Gossypium/growth & development , Molecular Sequence Data , Plant Proteins/chemistry , Plant Proteins/metabolism , Plant Roots/drug effects , Plant Roots/growth & development , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , Sodium Chloride/pharmacologyABSTRACT
Plant aquaporins, belonging to the MIP superfamily, are a series of transmembrane proteins that facilitate water transport through cell membranes. In this study, a cDNA clone encoding the PIP1-like protein was isolated from cotton (Gossypium hirsutum) cDNA libraries, and designated as GhAQP1 (Fig.1). We also isolated the GhAQP1 gene from cotton genome by PCR. The gene is 2,096 bp in length, including an open reading frame (ORF) and 5'-/3'-untranslated regions (UTR). It contains two introns in its ORF. The first intron is inserted between codons 209 and 210 in the fifth transmembrane helix, and another is located between codons 256 and 257 in the sixth transmembrane helix of GhAQP1, respectively (Figs.2 and 3). Northern blot analysis showed that GhAQP1 gene is expressed specifically in 6-15 DPA ovule, and reaches a peak in 9 DPA ovule (Figs.4 and 5), suggesting that its expression is ovule-specific and developmentally regulated in cotton.