Vertebral column regionalisation in Chinook salmon, Oncorhynchus tshawytscha.

Teleost vertebral centra are often similar in size and shape, but vertebral-associated elements, i.e. neural arches, haemal arches and ribs, show regional differences. Here we examine how the presence, absence and specific anatomical and histological characters of vertebral centra-associated elements can be used to define vertebral column regions in juvenile Chinook salmon (Oncorhynchus tshawytscha). To investigate if the presence of regions within the vertebral column is independent of temperature, animals raised at 8 and 12 °C were studied at 1400 and 1530 degreedays, in the freshwater phase of the life cycle. Anatomy and composition of the skeletal tissues of the vertebral column were analysed using Alizarin red S whole-mount staining and histological sections. Six regions, termed I-VI, are recognised in the vertebral column of specimens of both temperature groups. Postcranial vertebrae (region I) carry neural arches and parapophyses but lack ribs. Abdominal vertebrae (region II) carry neural arches and ribs that articulate with parapophyses. Elastic- and fibrohyaline cartilage and Sharpey's fibres connect the bone of the parapophyses to the bone of the ribs. In the transitional region (III) vertebrae carry neural arches and parapophyses change stepwise into haemal arches. Ribs decrease in size, anterior to posterior. Vestigial ribs remain attached to the haemal arches with Sharpey's fibres. Caudal vertebrae (region IV) carry neural and haemal arches and spines. Basidorsals and basiventrals are small and surrounded by cancellous bone. Preural vertebrae (region V) carry neural and haemal arches with modified neural and haemal spines to support the caudal fin. Ural vertebrae (region VI) carry hypurals and epurals that represent modified haemal and neural arches and spines, respectively. The postcranial and transitional vertebrae and their respective characters are usually recognised, but should be considered as regions within the vertebral column of teleosts because of their distinctive morphological characters. While the number of vertebrae within each region can vary, each of the six regions is recognised in specimens of both temperature groups. This refined identification of regionalisation in the vertebral column of Chinook salmon can help to address evolutionary developmental and functional questions, and to support applied research into this farmed species.

Gastric dilation and air sacculitis in Chinook salmon, Oncorhynchus tshawytscha (Walbaum); correlation of macroscopic and microscopic lesions, and relationship of the syndrome to glomerulonephritis and serum biochemistry.

Macroscopic and microscopic assessment procedures were developed to evaluate the severity and enable diagnosis from histological samples, of gastric dilation and air sacculitis (GDAS) in Chinook salmon, Oncorhynchus tshawytscha. Stomachs and swim bladders were examined from young fish with experimentally induced GDAS and from larger fish with the syndrome held in commercial saltwater net-pens. Fish fed a diet previously known to induce GDAS had significantly wider stomachs with decreased prominence of longitudinal stomach folds that contained larger amounts of fluid (P < 0.001), and thinner stomach walls with greater inter-nerve distances (P < 0.001), than fish fed an alternative commercial diet not associated with the syndrome. These fish also had swim bladders that were more likely to be opaque and contain more fluid (P < 0.001). These observations correlated well with selected criteria for stomach tissue (P < 0.002) and swim bladder (P < 0.04) that could be evaluated microscopically. Four stomach measurements, primarily measurements of wall or partial wall thickness and inter-nerve distances, were suitable for discriminating between affected and non-affected fish. A stomach width ratio, that was independent of fish weight and highly correlated with macroscopic stomach measurements (P < 0.0001), was particularly useful; this ratio was derived from the distances between both the outer border of the muscularis mucosa and mesothelium of the serosal surface to the stratum compactum. Serum biochemistry parameters (osmolality, calcium and magnesium) did not differ between fish fed different diets, but serum creatinine concentration was correlated with the microscopic thickness of the muscularis externa of the stomach wall and the total stomach thickness (P < 0.001 and P < 0.003, respectively). A glomerulonephritis was also noted in these fish. The severity of the lesion was not significantly related to GDAS nor to any serum biochemistry parameter assayed however.

Nodular gill disease causing proliferative branchitis and mortality in Chinook salmon (Oncorhynchus tshawytscha).

CASE HISTORY: A period of escalating mortality occurred among Chinook salmon (1-5 g) held in freshwater raceways at a commercial culture facility. The mortality rate peaked at 1.5% of the population per day, water temperature was 9-10 degrees C, and water quality had recently deteriorated due to an influx of suspended solids. The affected fish did not respond to several chloramine-T bath treatments (10 ppm for 1 hour). CLINICAL FINDINGS: The dead fish did not exhibit flared opercula typical of infection with bacterial gill disease (BGD) nor did the remaining fish show obvious signs of respiratory distress. Clinical lesions were limited to the gills of the fish, and were consistent with a proliferative branchitis, including substantial hyperplasia and fusion of lamellae. DIAGNOSIS: Numerous angular amoebic organisms (approximately 10 x 8 microm), with a central, large vesicular nucleus and faintly acidophilic cytoplasm, visible on H&E-stained sections, were located on the hyperplastic epithelia of the lamellae, filaments, and remaining lining of the branchial cavity. Morphologically, the amoebae were similar to representatives of the genus Cochliopodia spp. described from North American salmonids, that presented similar lesions and clinical signs; consequently, the disease was diagnosed as nodular gill disease (NGD). CLINICAL RELEVANCE: Grossly, NGD can be mistaken for BGD; the distinction is important because chloramine-T, routinely used in the treatment of BGD, is ineffective for the treatment of NGD. Static formalin baths of 170 ppm (37% formaldehyde) for 1 hour, supplemented with oxygen, may reduce amoebic infections but should be used cautiously as treatment can precipitate mortality in fish with gill disease. Cues for the onset of NGD are not completely understood, although poor water quality and elevations in temperature are likely to exacerbate infections. Similarly, the prevalence of this disease is not accurately known, but it is possible that it may be more common among salmonids in New Zealand than is presently recognised.

An immune-complex glomerulonephritis of Chinook salmon, Oncorhynchus tshawytscha (Walbaum).

Chinook salmon from New Zealand were shown to have a generalized membranous glomerulonephritis that was most severe in large fish. Marked thickening of the glomerular basement membrane was the most consistent lesion, with the presence of an electron-dense deposit beneath the capillary endothelium.Severely affected glomeruli also had expansion of the mesangium and loss of capillaries,synechiae of the visceral and parietal epithelium and mild fibrosis of Bowmans capsule. Chinook salmon from British Columbia, Canada with bacterial kidney disease caused by Renibacterium salmoninarum had similar histological lesions. They also had thickened glomerular basement membranes that were recognized by rabbit antiserum to rainbow trout immunoglobulin. This was true only when frozen sections of kidney were used and not formalin-fixed tissue. An attempt to experimentally produce a glomerulopathy in rainbow trout by repeated immunization with killed R. salmoninarum was not successful. Case records from the Fish Pathology Laboratory at the University of Guelph over a 10-year period revealed that a range of species were diagnosed with glomerulopathies similar to those seen in Chinook salmon. The majority of these cases were determined to have chronic inflammatory disease. This report has identified the presence of immunoglobulin within thickened basement membranes of Chinook salmon with glomerulonephritis and supports the existence of type III hypersensitivity in fish.

Experimental production of gastric dilation and its association with osmoregulatory stress and biogenic amines in chinook salmon, Oncorhynchus tshawytscha (Walbaum).

Chinook salmon smolt in fresh water fed a commercial diet known to produce minimal gastric dilation and air sacculitis (GDAS) were randomly assigned to four experimental tanks with flow-through sea water. All four groups were acclimatized to sea water for 3 weeks and fed a diet of minced fresh seafood. After 3 weeks the groups were fed either; seafood as before, a different commercial pelleted diet associated with the development of GDAS on farms, or either diet supplemented with 500 mg L(-1) putrescine, 300 mg L(-1) cadaverine and 250 mg L(-1) tyramine. Gastric dilation was produced in fish fed the commercial diet for 1 month but not by feeding a diet of minced seafood. The addition of putrescine, cadaverine and tyramine to either diet had no significant effect on the development of gastric dilation. Fish fed the commercial diet had significantly (P < 0.0001) wider weight-adjusted stomach widths, less prominent longitudinal stomach folds (P < 0.0001) and lower (P < 0.0001) stomach-width ratios than fish fed the fresh seafood diet. There was no significant difference in serum osmolality or sodium concentration between fish from groups with or without gastric dilation or fed biogenic amines.