ABSTRACT
Periodontal disease is a common infectious disease that can lead to the loss of teeth. Hower how to effectively suppress the inflammation with medication is unclear. The aim of the present study was to investigate the antiinï¬ammatory effect of Oroxylin A in periodontitis and its potential role through heme oxygenase1 (HO1). Primary rat gingival fibroblasts (RGFs) were cultured using the tissue block method and identified by immunofluorescence. Following lipopolysaccharide (LPS) stimulation of RGFs, Oroxylin A was administered at 50, 100, 200 or 400 µg/ml. Reverse transcriptionquantitative PCR was used to assess mRNA expression of cyclooxygenase (COX)2, TNFα, RANKL and osteoprotegerin (OPG). Western blotting was used to detect protein expression levels of COX 2, TNFα, RANKL and OPG. Following HO1 knockdown, the same treatment was performed. The expression of COX2 in rat gingival tissue was observed by immunohistochemistry. Oneway analysis of variance and Student's t test were used for statistical analysis. Oroxylin A downregulated mRNA expression of COX2, TNFα, RANKL and OPG in LPSinduced RGFs. With increase of Oroxylin A dose, the expression of HO1 was gradually upregulated. When HO1 was knocked down, Oroxylin A did not downregulate the expression of COX2, TNFα, RANKL and OPG in LPSinduced RGFs. Immunohistochemical results showed that expression of COX2 was downregulated by Oroxylin A, and the expression of TNFα, RANKL and OPG were also downregulated. Oroxylin A decreased expression of inflammatory cytokines in LPSinduced RGFs and had a good inhibitory effect on periodontitis in rats.