ABSTRACT
Traditional Chinese medicine (TCM) aromatherapy regulates the internal qi of the human body through the selection of specific aromatic medicinals, promoting the normal transportation and circulation of qi and blood and the coordination of zang-fu organs, and at the same time focusing on spiritual harmonization and nourishment, to achieve the therapeutic goal of nourishing both body and spirit. Its theoretical characteristics include the holism view of the unity of human beings and nature, the dynamic view of the balance of yin and yang, the pattern differentiation view of the three categories of disease cause, the preventive view of treating disease before it arises, and the regulation-care view of treating both body and spirit. From the perspective of qi theory, the theoretical connotation of TCM aromatherapy was analysed in depth, including the concept of life and disease, the concept of qi and blood, the theory of qi movement, and stressed the embodiment of the trinity view of body-qi-spirit in TCM aromatherapy, and the significant guidance of the principle of seeking the similar qi in TCM aromatherapy practice. This paper systematically summarized the theoretical characteristics and connotations of TCM aromatherapy, which could provide guidance for the practice of TCM aromatherapy.
ABSTRACT
Swept-source optical coherence tomography angiography(SS-OCTA)is a new vascular imaging technique that was recently proposed. It has the advantages of being non-invasive, quick, high-resolution, and automated vascular stratification imaging. It is extremely helpful in the early diagnosis of ophthalmology-related diseases, as well as in the evaluation of treatment effectiveness and the tracking of disease progression. Based on the foundation of OCTA, SS-OCTA utilizes a fast-tuning laser with a wavelength of 1 050 nm for deeper penetration and non-invasive depth-resolved imaging of the retinal and choroidal microvascular systems, deepening the understanding of the characteristics of a wide range of ophthalmic diseases(fundus lesions, glaucoma, neurodegenerative diseases, etc.). The structures of the anterior segment of the eye can also be studied using SS-OCTA, including changes in the depth and density of corneal neovascularization as well as changes in iris neovascularization before and after therapy. This approach provides a novel tool for ophthalmic clinical practice. The development of the clinical use of SS-OCTA technology in ophthalmology is reviewed in this article.
ABSTRACT
Type 2 diabetes mellitus (T2DM) is a heterogeneous disease with insulin deficiency and insulin resistance (IR) as the main etiology and is often accompanied by complications. Volatile oil is a volatile oily liquid extracted from natural plants, which has many pharmacological effects such as regulating Qi, relieving pain, inhibiting bacteria, and reducing inflammation. In recent years, there have been numerous reports on the treatment of T2DM by natural plant volatile oil and its effective components, which has become one of the new directions in the treatment of T2DM. With natural plant essential oil and its active components as the starting point, this paper comprehensively analyzed and summarized the material basis, mechanism, and signaling pathways of essential oil in the treatment of T2DM and its complications in China and abroad in recent years, and focused on the inhibitory effect of essential oil and its active components, such as carvacrol, paeonol, and β-caryophylene, on IR to improve T2DM by protecting pancreatic β-cells, inhibiting α-glucosidase activity, regulating the abundance and diversity of intestinal microbiota, and regulating glucose transporter protein type4 (GLUT4), adenylate 5′-monophosphate-activated protein kinase (AMPK), phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt) signaling pathways to provide some references for the volatile oil intervention in T2DM and the development of new green antidiabetic drugs.
ABSTRACT
AIM: To investigate the effect of oxypeucedanin (OPD) on doxorubicin resistance in human breast cancer MCF-7/DOX cells and its possible mechanism. METHODS: MCF-7/DOX cells were cultured in vitro, MTT assay was used to detect the effect of OPD on the survival of MCF-7/DOX cells, and the effect of OPD combined with different concentrations of doxorubicin on the proliferation of MCF-7/DOX cells were investigated. The effect of OPD combined with doxorubicin on the expression of genes including MDR1, MRP1, AGPAT2, CHKA, CEPT1, DGKA, PCYT1A, PLA2G15 in MCF-7/DOX cells was measured by qRT-PCR. The effect of OPD combined with doxorubicin on the protein expression of MDR1, MRP1, CHKA and CCTα in MCF-7/DOX cells was determined by Western blot. RESULTS: The IC
ABSTRACT
Objective:To investigate the effects of graded exposure in vivo treatment on kinesiophobia and resilience in patients with coronary heart disease, to provide reference for clinical nursing care.Methods:A total of 86 coronary heart disease patients from January 2018 to October 2019 in the First People′s Hospital of Jingzhou were randomly divided into experimental group and control group with 43 cases in each group according to the random number table method. The control group received routine nursing, while the experimental group implemented graded exposure in vivo treatment addition to routine nursing. The results of intervention were assessed by the Tampa Scale for Kinesiophobia Heart (TSK-SV Heart) and Connor-Davidson Resilience Scale (CD-RISC), respectively.Results:After intervention, the scores of perceived danger, fear of injury, avoidance of exercise, dysfunctional self dimension and total socres in TSK-SV Heart were (5.45 ± 1.13), (6.55 ± 1.62), (6.92 ± 1.32), (6.42 ± 1.14), (25.34 ± 3.91) points in the experimental group, significantly lower than those in the control group (7.20 ± 1.91), (7.80 ± 2.30), (8.15 ± 1.85), (7.98 ± 1.44), (31.13 ± 4.47) points, the differences were statistically significant ( t values were 2.60-6.07, all P<0.05). The scores of tenacity, strength, optimism dimension and total scores in CD-RISC were (19.58 ± 5.29), (19.68 ± 5.16), (12.76 ± 4.81), (52.03 ± 8.64) points in the experimental group, significantly higher than those in the control group (17.43 ± 3.95), (16.80 ± 3.61), (10.28 ± 3.98), (44.50 ± 6.24) points, the differences were statistically significant ( t values were 2.03-4.39, all P<0.05). Conclusions:Graded exposure in vivo treatment can effectively alleviate kinesiophobia and promote resilience of patients with coronary heart disease.
ABSTRACT
Breast cancer is a systemic malignant tumor caused by multiple pathogenic factors, and its pathological mechanism is complex and has not been clarified so far. It has gradually become the largest killer threatening women's life. The common method for the treatment of breast cancer is lesion resection combined with radiation and chemical therapy, endocrine therapy, or targeted therapy. However, due to the limitations of western medicine therapies, there are still considerable breast cancer patients with poor disease control and high tumor recurrence rate in clinical practice. At the same time, the side effects and complications produced by these therapies affect the quality of life of patients. Therefore, it is urgent to develop new drugs or find safe and effective alternative therapies against breast cancer. Volatile oil (VO), as a unique volatile component of Chinese herbal medicines, has anti-inflammatory, antiviral, and anti-tumor activities. It has been applied in the treatment of breast cancer and has demonstrated good efficacy by exerting the unique effects of strengthening healthy Qi, eliminating pathogenic factors, moving Qi, resolving stasis, warming Yang, soothing liver, and relieving depression. The recent studies have confirmed that VO and its chemical components can prevent and treat breast cancer via multiple mechanisms, while there is a lack of systematic review. The relevant literature published in recent years has demonstrated that VO can inhibit the occurrence and development of breast cancer by regulating the level of estrogen, inhibiting the proliferation and inducing the apoptosis of cancer cells, enhancing immunity, resisting inflammation, and regulating emotions. We introduced the pathogenesis of breast cancer, as well as the mechanisms and advantages of VO in the prevention and treatment of breast cancer, aiming to provide new ideas for the research on VO in the treatment of breast cancer.
ABSTRACT
【Objective】 To investigate the impact of ceasing mutual blood donation on voluntary blood donation in Guangzhou. 【Methods】 The data of blood donation from July 2016 to December 2019 (42-month before and after the official cease of mutual blood donation) in the Blood Collection and Supply System of Guangzhou Blood Center, including whole blood donations and apheresis platelets donations, were collected for interrupted time series analysis by month. Blood donors who donated (either whole blood or platelets) during 2016 were followed up until December 31, 2019, and the re-donation rate was analyzed by Chi-square test, t test and logistic regression analysis. 【Results】 The results showed that ceasing mutual blood donation had a significantly positive effect on the increase of platelet donations, but had no significant effect on whole blood donation. In 2016, whole blood donations and platelet donations were mainly voluntary (86.4% and 60.8%, respectively). In comparison of voluntary blood donation, the overall blood deferral rate(by dual assays) of mutual blood donation was higher (P<0.01), but the difference diminished as they donated twice or more. The re-donation rate of blood donors (mutual non-remunerated, voluntary, or both) all increased after the ceasing of mutual blood donation (mutual non-remunerated, : 4.7% vs 4.0%, χ2=29.8, P<0.01; voluntary: 24.8% vs 9.9%, χ2=17295.3, P<0.01; both: 36.3% vs 28.1%, χ2=29.3, P<0.01). The re-donation rate of mutual platelet donors decreased after the ceasing of mutual blood donation, but the number of voluntary platelet donors increased. 【Conclusion】 The ceasing of mutual blood donation was in favour of voluntary blood donation in Guangzhou since various means had been previously adopted by Guangzhou Blood Center to create a long-term mechanism of voluntary blood donation. The number of voluntary blood donors has increased, and the clinical use of blood has been further guaranteed.
ABSTRACT
【Objective】 To explore the effect of questionnaires on the re-recruitment of lapsed blood donors, and to ensure the retention of regular blood donors for blood supply in blood stations. 【Methods】 Blood Donation Motivation Questionnaire and Blood Donation Deterrents Questionnaire were designed for inactive and lapsing blood donors to inquire the motivation of the latest blood donation, such as "blood donation can save lives" and the reasons for no longer participating in blood donation, such as "there are no blood donation sites nearby", respectively. 13 093 blood donors with donation frequency ≥3 times and last donation during January 1 to May 17, 2018 in Guangzhou were selected as subjects. Text messages containing the links to the correspondent electronic questionnaires were sent to intervention group 1 (n=4 364) to fulfill the Blood Donation Motivation Questionnaire and intervention group 2 (n=4364) to fulfill Blood Donation Deterrents Questionnaire from May 18 to 25, 2020. None questionnaire was issued to the control group (n=4 365). The re-donation rates in the three groups within 2 months after the questionnaire delivery were analyzed by intention to treat (ITT) analysis and average treatment effect (ATT) estimation. 【Results】 The response rate of valid questionnaires was 5.422% (710/13 093), of which 7.424% (324/4 364) were in intervention group 1 and 8.845% (386/4 364) in intervention group 2. The collected questionnaire showed that the score of "blood donation can save lives" was the highest (2.31±0.79)in intervention group 1, and the score of "no blood donation site nearby" was the highest (2.31±0.80). in intervention group 2.2 months of observation showed that the re-donation rate was similar among all three groups by ITT analysis (Ps>0.05). ATT estimation results showed that the re-donation rates of intervention group 1 and intervention group 2 were 5.56%(18/324) and 3.11%(12/386), respectively(P<0.05). 【Conclusion】 Motivation questionnaire is a simple and convenient way to remind blood donors who have multiple donations to donate blood again.
ABSTRACT
Objective:To explore the value of radiographic assessment of lung edema (RALE) score in evaluating the severity and prognosis of patients with acute respiratory distress syndrome (ARDS).Methods:A retrospective study was conducted. Patients with ARDS admitted to the department of intensive care unit (ICU) of Affiliated Nantong Third Hospital of Nantong University from January 2016 to November 2020 were enrolled. Clinical data of those patients were collected, and two senior radiologists who did not know the outcome of the patients independently scored each chest radiograph, the mean value of which was taken as the RALE score. The patients were divided into death group and survival group according to the 28-day prognosis. The differences of the basic data, PaO 2/FiO 2, sequential organ failure assessment (SOFA) score, acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) score and RALE score between groups were analyzed. ARDS patients were classified according to the Berlin standard and RALE scores were compared between groups. Then, the correlations between RALE score and PaO 2/FiO 2, SOFA score, APACHEⅡ score were analyzed. The prognostic capacity of RALE score for 28-day prognosis of ARDS patients were analyzed by Kaplan-Meier survival curve. Results:Of the 98 ARDS patients, 62 were included in the final analysis, 39 patients survived and 23 patients died. The 28-day mortality was 37.1%. Compared with the survival group, patients in the death group were older (years old: 72.83±12.21 vs. 64.44±14.68), had lower PaO 2/FiO 2 [mmHg (1 mmHg = 0.133 kPa): 122.66±48.32 vs. 150.26±50.40], and higher SOFA score and greater difference of RALE score between the third day and the first day after admission (D3-D1 RALE score) (SOFA score: 11.26±3.91 vs. 9.04±3.72, D3-D1 RALE score: 1.35±6.42 vs. -2.74±7.35), with statistically significant differences (all P < 0.05). However, there were no significant differences in gender, cause of ARDS, APACHEⅡ score, and RALE scores on the first and the third day of admission (D1 RALE, D3 RALE) between the two groups. Among the 62 patients, there were 11 mild cases (17.7%), 36 moderate cases (58.1%), and 15 severe cases (24.2%). The D1 RALE score of patients with mild and moderate ARDS were lower than those of patients with severe ARDS (19.09±3.65, 22.58±6.79 vs. 27.07±5.23, both P < 0.05). Correlation analysis showed that D1 RALE score was negatively correlated with PaO 2/FiO 2 ( r = -0.385, P = 0.002), and positively correlated with SOFA score and APACHEⅡ score ( r1 = 0.433, r2 = 0.442, both P < 0.001). Kaplan-Meier survival curve analysis showed that the 28-day survival rate of ARDS patients in D3-D1 RALE score ≥ -1 group was significantly higher than that in D3-D1 RALE score < -1 group (73.08% vs. 55.56%; log-rank test: χ 2 = 3.979, P = 0.046). Conclusions:The RALE score is a simple and reliable non-invasive evaluation index, which can be used to evaluate the severity of ARDS patients. The difference of RALE score in early stage is helpful to identify ARDS patients with poor prognosis.
ABSTRACT
Objective:To explore the application value of artificial intelligence (AI) in image post-processing of reconstructed CTA based on CT cerebral perfusion (CTP).Methods:Clinical and radiological data of 100 patients suspected of cerebrovascular diseases in Hebei General Hospital from January to July 2020 were retrospectively selected. All patients were divided into A and B group on average according to the different examination schemes. Cerebral CTP examination was performed in group A (the temporal maximum intensity projective data set generated by the first 5 time phases in the maximum period of the difference between arteriovenous CT values selected as subgroup A1, and the corresponding original thin-layer images selected as subgroup A2), single phase CTA examination was performed in group B, manual and AI image post-processing were performed respectively. Subjective scoring of the image data was performed, and the objective bid evaluation indexes such as CT value, noise (SD), signal-to-noise ratio (SNR), contrast to noise ratio (CNR) were measured, the qualified rate of artificial and AI vascular segmentation was counted, and post-processing time were recorded. The objective evaluation indexes were compared between three groups using one-way ANOVA, and the Kruskal-Wallis H test was used to compare the difference of subjective scores.Results:Statistically significant differences were observed in subjective score and objective evaluation index of original images among group A1, group A2 and group B (all P<0.05). Among them, arterial enhancement, arteriolar detail display score, cerebral artery CT value, SNR and CNR in group A1 were higher than those in group A2 and group B (all P<0.05). In a total of 100 patients with 1 100 blood vessels, the qualified rates of AI vascular segmentation in group A1 [98.4% (541/550)] and group B [98.7% (543/550)] were higher than those of manual [82.9% (456/550), 87.1% (479/550), χ2=77.392, 56.521, P<0.001], but the qualified rate of AI vascular segmentation of group A2 [78.4% (431/550)] was lower than that of manual [85.6% (471/550), χ2=9.855, P=0.002]. The completion time of AI post-processing were reduced by 56.30%, 49.63%, 50.81%, respectively than those with manual. Conclusion:Compared with manual image post-processing, AI has certain advantages in image quality and work efficiency of reconstructed CTA post-processing based on CTP de-noising dataset, and it is worth popularizing and applying in the image post-processing of cerebrovascular disease, combined with artificial quality control.
ABSTRACT
Objective:To investigate the feasibility of one-step coronary and carotid-cerebrovascular computed tomography angiography (CTA) using high-pitch Double Turbo Flash mode and to analyze the image quality and radiation dose in patients with heart rate variability using multi-slice detector dual-source CT.Methods:A total of 79 patients with heart rate variability higher than 3 beat/min (bpm) were retrospectively analyzed. They were grouped by the scanning methods. Group A ( n=40) were performed double turbo flash mode for one-step coronary and carotid-cerebrovascular arteriesinjected with one injection of contrast agent.further divided into Single A (only the phase 1 image)and Double A(combined images of phases 1 and 2). Group B ( n=39) were performed separately with twice injection of contrast agent. Subjective scoring was performed on the image quality of the group Single A, Double A and B using a 4-point. Then the objective parameters of image quality, CT attenuations, image noise, signal-to-noise (SNR), and contrast-to-noise (CNR), were evaluated. Then subjective scores, objective evaluation indicators, and radiation dose were compared with one-way ANOVA analysis among the three groups.Contrast agent were compared by using t test in group A and B. Results:No significant difference was found ( F=2.093, P=0.128) for the subjective scores of the head and neck CTA among the three groups with (3.47±0.51), (3.53±0.51), (3.69±0.47). Significant difference was found ( F=50.955, P<0.01) for the subjective scores of coronary CTA among the three groups with (2.70±0.76), (3.35±0.66), (3.58±0.50), and there was no statistically significant difference between Double A and B groups ( P=0.104), met the requirements for diagnosis. The objective evaluation of the three groups compared the head and neck CTA images were statistically significant ( P<0.05), of which the difference between the Single A and the Double A was not statistically significant ( P>0.05), met the requirements for diagnosis; the differences in SNR and CNR of coronary CTA images are statistically significant ( F=12.991, 12.236, P<0.01), and there was no statistically significant difference between Double A and B ( P>0.05), met the requirements for diagnosis. Radiation dose in the group Double A was lower than group B (decreased by 46.15%, P<0.01).The amount of the contrast agent in the group Double A was lower than group B (decreased by 44.13%, t=-45.455; P<0.01). Conclusion:The double turbo flash mode is feasibility for one-step coronary and carotid-cerebrovascular CTA in patients with the heart rate variability using multi-slice dual-source CT.This scan mode can maintain the diagnostic image quality with low contrast agent and radiation dose.
ABSTRACT
Objective@#To investigate the feasibility of one-step coronary and carotid-cerebrovascular computed tomography angiography (CTA) using high-pitch Double Turbo Flash mode and to analyze the image quality and radiation dose in patients with heart rate variability using multi-slice detector dual-source CT.@*Methods@#A total of 79 patients with heart rate variability higher than 3 beat/min (bpm) were retrospectively analyzed. They were grouped by the scanning methods. Group A (n=40) were performed double turbo flash mode for one-step coronary and carotid-cerebrovascular arteriesinjected with one injection of contrast agent.further divided into Single A (only the phase 1 image)and Double A(combined images of phases 1 and 2). Group B (n=39) were performed separately with twice injection of contrast agent. Subjective scoring was performed on the image quality of the group Single A, Double A and B using a 4-point. Then the objective parameters of image quality, CT attenuations, image noise, signal-to-noise (SNR), and contrast-to-noise (CNR), were evaluated. Then subjective scores, objective evaluation indicators, and radiation dose were compared with one-way ANOVA analysis among the three groups.Contrast agent were compared by using t test in group A and B.@*Results@#No significant difference was found (F=2.093, P=0.128) for the subjective scores of the head and neck CTA among the three groups with (3.47±0.51), (3.53±0.51), (3.69±0.47). Significant difference was found (F=50.955, P<0.01) for the subjective scores of coronary CTA among the three groups with (2.70±0.76), (3.35±0.66), (3.58±0.50), and there was no statistically significant difference between Double A and B groups (P=0.104), met the requirements for diagnosis. The objective evaluation of the three groups compared the head and neck CTA images were statistically significant (P<0.05), of which the difference between the Single A and the Double A was not statistically significant (P>0.05), met the requirements for diagnosis; the differences in SNR and CNR of coronary CTA images are statistically significant (F=12.991, 12.236, P<0.01), and there was no statistically significant difference between Double A and B (P>0.05), met the requirements for diagnosis. Radiation dose in the group Double A was lower than group B (decreased by 46.15%,P<0.01).The amount of the contrast agent in the group Double A was lower than group B (decreased by 44.13%, t=-45.455;P<0.01).@*Conclusion@#The double turbo flash mode is feasibility for one-step coronary and carotid-cerebrovascular CTA in patients with the heart rate variability using multi-slice dual-source CT.This scan mode can maintain the diagnostic image quality with low contrast agent and radiation dose.
ABSTRACT
Objective To explore the effects of improved emergency nursing intervention on complications and clinical prognosis of severe patients with craniocerebral injury accompanied by cerebral hernia, and provide evidence for clinical nursing. Methods The patients with severe craniocerebral injury accompanied by cerebral hernia were enrolled in Dongguan People's Hospital from January 2016 to December 2018. According to the admission time, the patients were divided into routine nursing group (Admission from January 2016 to June 2017) and improved emergency nursing methods group (Admission from July 2017 to December 2018). To analyze the effect of two different nursing methods on the prognosis of patients by improving first aid nursing measures and routine nursing. Both groups of patients underwent craniotomy, the conventional nursing group was treated with routine operating room care, the improved emergency nursing methods group was given improved emergency care interventions based on the conventional nursing, the operation preparation time, operation time, intra-operative blood loss and the incidence of postoperative complications, clinical prognosis of the two groups were observed; the self-made questionnaire was used to evaluate the scores of the patient's satisfaction and compliance. Results The operation preparation time, operation time and intra-operative blood loss of the improved emergency nursing methods group (40 cases) were significantly lower than those in the conventional nursing group [40 cases, operation preparation time (minutes): 25.03±5.47 vs. 36.73±5.83, operation time (minutes): 52.56±10.63 vs. 92.10±10.73, intra-operative blood loss (mL): 218.73±20.74 vs. 402.66±23.70, all P < 0.05]; the clinical good prognosis rate, rate of satisfaction to nursing, treatment compliance score of the improved emergency nursing methods group were significantly higher than those of the conventional nursing group [clinical good prognosis rate: 20.0% (8/40) vs. 12.5% (5/40), rate of satisfaction to nursing: 95.0% (38/40) vs. 82.5% (33/40), treatment compliance scores: 95.83±4.09 vs. 81.27±5.04, all P < 0.05]; the complication rate of the improved emergency nursing methods group was significantly lower than that of the conventional nursing group [32.5% (13/40) vs. 55.0% (22/40), P <0.05]. Conclusion The improved emergency nursing intervention can significantly improve the clinical prognosis and reduce the incidence of complications in patients with severe craniocerebral injury accompanied by cerebral hernia, so that the clinical application value is relatively high.
ABSTRACT
Objective To know the relationship between the uterine fibroid symptom severity and the quality of life andthe correlation among all the subscales of the quality of life base through analysis of the questionnaires of the uterine fibroids health-related quality of life.Methods Retrospectively analyze the UFS-QOL subscales and SF-36 subscales of the 302 women with uterine fibroids who underwent ultrasound ablation in the high intensity focused ultrasound center of the First Affiliated Hospital of Chongqing Medical University in 2013.Results The Cronbach'sa of the UFS-QOL symptom severity subscale and the HRQL subscales were 0.794 and 0.953,reflecting adequate internal consistency.Except the sexual function field,the symptomatic group reported significantly greater symptom severity and worse health-related quality of life(HRQL) than the asymptomatic group(P< 0.05).Spearman's correlations between the symptom severity subscale and each HRQL subscales were moderately in the asymptomatic group,there was a linear positive correlation between the UFS-QOL subscales of activities,energy/mood and control(P< 0.05).In the symptomatic group,there was a linear negative correlation between the symptom severity subscale and the UFS-QOL subscales of concern,activities,energy/mood(P<0.05),and there was a linear positive correlation between the UFS-QOL subscale of activities and the UFS-QOL subscales of concern,energy/mood.Conclusion Uterine fibroids related symptoms have an impact on the quality of life in women with uterine fibroids,and the assessments among the subscales of the quality of life influence each other.
ABSTRACT
Objective To analyze the comparation of national early warning score (NEWS), rapid emergency medicine score (REMS) and acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ) score in predicting prognosis of critically ill patients in emergency department (ED). Methods A retrospective study was conducted. Critically ill patients, aged > 16 years, hospitalized > 24 hours, and admitted to the ED of Nanhua Hospital Affiliated to South China University from January 2016 to June 2017 were enrolled. NEWS, REMS and APACHE Ⅱ score were calculated based on the worst value of each index within 24 hours after emergency admission. The primary endpoint was 28-day mortality. The relationship between the three scoring systems and the prognosis of patients was analyzed. The predictive value of three scoring systems for the prognosis of critically ill patients in ED was analyzed by receiver operating characteristic curve (ROC). Results A total of 119 emergency severe patients were enrolled in the study, and the 28-day mortality was 21.0%. The scores of NEWS, REMS and APACHE Ⅱ in the death group were significantly higher than those in the survival group (NEWS score: 9.40±3.19 vs. 5.72±2.35, REMS score: 12.64±4.46 vs. 7.97±3.28, APACHE Ⅱscore: 26.64±6.92 vs. 16.19±5.48, all P < 0.01). With the increase of NEWS, REMS and APACHE Ⅱ score, the 28-day mortality of patients gradually increased [28-day mortality of NEWS < 5, 5-6, ≥ 7 was 3.03% (1/34), 13.33% (4/34), 64.25% (20/51); 28-day mortality of REMS < 12, 12-16, ≥ 17 was 10.99% (10/91), 50.00% (11/22), 66.67% (4/6); 28-day mortality of APACHE Ⅱ < 15, 15-24, ≥ 25 was 2.33% (1/43), 15.09% (8/59), 69.57% (16/23), respectively, all P < 0.01]. The ROC curve analysis showed that the areas under the ROC curve (AUC)of NEWS, REMS and APACHE Ⅱ score for predicting the prognosis of emergency critically ill patients were 0.830 [95% confidence interval (95%CI) = 0.737-0.923], 0.782 (95%CI = 0.671-0.892) and 0.878 (95%CI = 0.800-0.956), respectively (all P = 0.000), and the accuracy of prediction was 57.4%, 48.6%, 65.4%, respectively. Conclusions The scores of NEWS, REMS and APACHE Ⅱ were useful in predicting prognosis of critically ill patients, with the highest accuracy of APACHE Ⅱ forecast, followed by NEWS, and the lowest of REMS. After comprehensive consideration of cost-effectiveness, NEWS is more reliable in ED.
ABSTRACT
Objective To investigate the effect of WeChat on nutrition instructions to women of advanced maternal age with gestational diabetes mellitus (GDM). Methods Eighty women of advanced maternal age with GDM in our hospital during January to December 2014 were divided according to their registration nubmer into two group in eqaul number: those with even number in the observation group and those with odd number in the control group. Those in the control group took part in the health educaiton lectures by the parturient school and those in the observation group were instructed on the WeChat besides education from the school. The two groups were compared in terms of blood glucose control and delivery outcomes. Result The level of blood glucose in the observation group was significantly lower than that of the control group (P0.05). Conclusion Wechat is effective in providing the women of advanced maternal age with nutrition instructions and beneficial for the control of blood glucose level and the decrease of cesarean delivery and vaginal birth rates.
ABSTRACT
Objective To discuss the effect of team-based learning(TBL)combined with net-work environment in pathological experiment teaching. Methods Totally 112 clinical medical under-graduates were selected as research object. Digital microscope interactive teaching platform under the network environment was used in experimental group(n=56). In pathology experiment teaching,TBL steps of training,teaching plan demonstrating,grouping,teaching task arranging,asking questions, group learning and communicating were implemented. Traditional method (lecture)was used in con-trol group (n=56). Teaching effect was analyzed through questionnaire survey and score analysis. Stu-dents' evaluation on TBL teaching and network environment resources was expressed as percentage. Results In experiment group,average score was (85.5±3.11)points and average final exam score was (83.8±2.53)points,while in control group,average final exam score was (76.4±11.89)points. There were significance differences between the two groups (t=7.018,P<0.01). Surveys showed that most students accepted TBL teaching combined with network environment. Conclusions TBL teach-ing combined with network environment is feasible and effective in the pathology experimental class. Students' learning enthusiasm is generally improved and effectiveness of TBL teaching is satisfactory.
ABSTRACT
AIM:To establish a fast , accurate and economical technique for culturing mouse pulmonary arte-riolar smooth muscle cells ( PASMCs ) , and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs.METHODS:In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask.Centrifugal procedure was not used dur-ing the cell passage .The cell morphology was observed under an inverted phase-contrast microscope .α-Smooth muscle ac-tin was identified by immunocytochemistry and immunofluorescence .The effects of hypoxia on the proliferation and apopto-sis of the PASMCs were detected by CCK-8 assay and TUNEL assay .RESULTS:PASMCs were identified by the methods of immunocytochemistry , immunofluorescence staining and observation of morphology .Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern.The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells .CCK-8 assay demonstra-ted that the A values of PASMCs exposed to hypoxia increased after 24 h ( P<0.05) as compared with normoxia .TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05).CONCLUSION:This technique for obtaining cultured mouse PASMCs is simple , fast, accurate and economical .The digestion time is easy to control.Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs .
ABSTRACT
To explore the effects of two breathholding and exerting methods on the second labor course of primiparas.Methods Six hundred primiparas were divided according to the admission time into two groups: the control group and observation group. The former took conventional breathholding and exerting method and the latter breathholding and exerting method with breathing techniques. The two groups were compared in terms of outcome of delivery, rate of episiotomy, time for breathholding and exerting and the hemorrhage volume 2h after delivery, perineal laceration and neonate asphyxia.Results The rate of spontaneous delivery in the observation group was higher than that in the control group. The rate of episiotomy, bleeding volume 2h after delivery and perineal laceration were lower. The breathholding time was significantly shorter(P<0.05).Conclusion Breathholding and exerting at the second stage of labor can reduce energetic consumption and improve the outcome of delivery.
ABSTRACT
AIMS: In the present study, we explored the hypothesis that initiation of PH involves the upregulation of monocyte chemoattractant protein-1 (MCP-1) in acute PTE. We evaluated the effects of resveratrol and the role of p38 mitogen-activated protein kinase (MAPK) in this process. MAIN METHODS: A rat model of acute PTE was established by infusion of an autologous blood clot into the pulmonary artery through a polyethylene catheter. Rats were randomly divided into 1, 4, and 8 hour time groups. Resveratrol, C1142 (a rodent chimeric mAb that neutralizes rat MCP-1) or SB203580 (a p38MAPK specific inhibitor) was administered to the animals beginning 1 h prior to the start of the acute PTE protocol. At each time point, the mean pulmonary artery pressure (mPAP), mRNA and protein expressions of MCP-1 were measured. The phosphorylation of p38 MAPK (p-pMAPK) was also detected. KEY FINDINGS: Acute PTE elicited significant increases in mean pulmonary artery pressure (mPAP), and up-regulated the expression of monocyte chemoattractant protein-1 (MCP-1) and phosphorylation of p38 mitogen-activated protein kinase (p-p38 MAPK). Administration of C1142 markedly reduced mPAP. Furthermore, pre-treatment of rats with resveratrol significantly reduced mPAP and down-regulated the expression of MCP-1, which was associated with robustly suppressed acute PTE-induced p-p38MAPK expression. SIGNIFICANCE: These findings suggested that MCP-1 was involved in the formation of acute PTE-induced PH, and resveratrol down-regulated the expression of MCP-1 by inhibiting acute PTE-induced p-p38MAPK activation, which contributed to the decrease in PH.