ABSTRACT
BACKGROUND: Our previous data suggested that three single-nucleotide polymorphisms (SNPs), rs1048661, rs3825942, and rs2165241, of the lysyl oxidase-like 1 gene (LOXL1) are significantly associated with exfoliation syndrome (XFS) and exfoliation glaucoma (XFG). The following study investigated other SNPs that potentially effect XFS/XFG. METHODS: A total of 216 Uygur patients diagnosed with XFS/XFG, and 297 Uygur volunteers were admitted to the First Affiliated Hospital at Xinjiang Medical University between January 2015 and October 2017. Blood samples were collected by venipuncture. Alleles and genotypes of LOXL1, TBC1D21, ATXN2, APOE, CLU, AFAP1, TXNRD2, CACNA1A, ABCA1, GAS7, and CNTNAP2 were analyzed by direct sequencing. RESULTS: The allele G of rs41435250 of LOXL1 was a risk allele for XFS/XFG (P < 0.001), whereas the allele G of rs893818 of LOXL1 was a protective allele for XFS/XFG (P < 0.001). After adjusting all data for age and gender, the following results were obtained: the frequency of genotype CC for rs7137828 of ATXN2 was significantly higher in XFS/XFG patients than in controls (P = 0.027), while no significance was found with reference to the frequency of genotype TT. The frequency of genotype GG for rs893818 of LOXL1 (P < 0.001) and the frequency of genotype AA were both significantly higher in XFS/XFG groups compared to the control group (P < 0.001). In addition, the frequency of genotype TT for rs41435250 of LOXL1 was higher in XFS/XFG patients than in controls (P = 0.003), while no significant difference was found with reference to the frequency of genotype GG after adjusting for age and gender. In addition, the haplotypes G-A/T-G/G-G for rs41435250 and rs893818 were significantly associated with XFS/G. CONCLUSIONS: With reference to LOXL1, the rs41435250 resulted as a risk factor and rs893818 as a protective factor for XFS/XFG in the Uygur populations. Meanwhile, the rs16958445 of TBC1D21 and the rs7137828 of ATXN2 have also shown to be associated with pathogenesis of XFS/XFG.
ABSTRACT
AIM: To study the histopathological changes in the retina and flash electroretinogram (F-ERG) features of ozone-treated streptozotocin (STZ)-induced diabetic rats. METHODS: Seventy male Sprague Dawley rats were grouped as follows: blank group (GB, n=10), model control group (GM, n=18), ozone group (GO3, n=19), and oxygen group (GO2, n=18). The model was induced by single intraperitoneal injection of STZ. Ozone or oxygen enteroclysm was given twice per week for 4wk. F-ERG and histopathological examinations were performed one month after treatment. RESULTS: Under dark adaption, as compared to GB, the other groups each had differential decreases in the a-wave amplitudes (P<0.05); the latencies were delayed in GM, GO2, and GO3 rats (P<0.05). Similar results were observed under light adaption, with the exception that the a-wave of the amplitudes (F=0.28, P>0.05). There were significant differences in the apoptosis index among the groups (P<0.05). Under ozone treatment, apoptosis was decreased in GO3 as compared to GM and GO2. CONCLUSION: Ozone administration alleviates nerve damage and reduces pathology and apoptosis in the retinas of diabetic rats.
ABSTRACT
OBJECTIVE: To estimate the prevalence of visual impairment among adults aged ≥ 40 years, compare the prevalence between Uyghur and Han residents and analyze the causes of visual impairment. METHODS: Population-based cluster sampling survey was conducted among a target population of 5032 persons ≥ 40 years of age from Shuimogou District in Urumqi, Xinjiang Uyghur Autonomous Region. Distance visual acuity (VA) was measured with a Tumbling-E chart at 5 m. Presenting and best corrected VA were recorded. Clinical evaluations were performed. And primary causes of visual impairment was determined for those with VA < 0.3. RESULTS: Among them, there were 4104 responders (Uyghur 2006 persons, Han 2098 persons) with a response rate 81.56%. Prevalence of bilateral blindness was 1.25%/1.86% (Uyghur/Han) (P < 0.05) and bilateral low vision 4.04%/3.29% (Uyghur/Han) (P < 0.05). Primary cause of visual impairment among participants was cataract (cause proportion of 49.23% among Uyghurs and 51.85% among Hans). Visual impairment was correlated with advanced age, males and poor literacy. Prevalences of bilateral blindness and bilateral low vision in age group 40 - 50 yr increased from 0.48% and 1.13% to 3.47% and 10.88% in age group ≥ 70 among Uygur citizens respectively (χ(2) = 22.36, P < 0.05 vs χ(2) = 70.97, P < 0.05). Relative data among the Han citizens varied from 0% and 1.42% to 4.39% and 7.89% (χ(2) = 27.94, P < 0.05 vs χ(2) = 39.99, P < 0.05). CONCLUSION: The most frequent cause of visual impairment in this area is cataract (Uyghur 49.23% vs Han 51.85%) while retinal diseases and glaucoma are important causes as well. Blindness prevention programs targeting the elderly should be expanded, particularly in areas with a limited access and affordability of eye care services.
