Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters











Database
Language
Publication year range
1.
FASEB J ; 35(8): e21723, 2021 08.
Article in English | MEDLINE | ID: mdl-34224609

ABSTRACT

Sperm acquire the ability to fertilize in a process called capacitation and undergo hyperactivation, a change in the motility pattern, which depends on Ca2+ transport by CatSper channels. CatSper is essential for fertilization and it is subjected to a complex regulation that is not fully understood. Here, we report that similar to CatSper, Cdc42 distribution in the principal piece is confined to four linear domains and this localization is disrupted in CatSper1-null sperm. Cdc42 inhibition impaired CatSper activity and other Ca2+ -dependent downstream events resulting in a severe compromise of the sperm fertilizing potential. We also demonstrate that Cdc42 is essential for CatSper function by modulating cAMP production by soluble adenylate cyclase (sAC), providing a new regulatory mechanism for the stimulation of CatSper by the cAMP-dependent pathway. These results reveal a broad mechanistic insight into the regulation of Ca2+ in mammalian sperm, a matter of critical importance in male infertility as well as in contraception.


Subject(s)
Calcium Channels/metabolism , Spermatozoa/metabolism , cdc42 GTP-Binding Protein/metabolism , Animals , Calcium/metabolism , Calcium Channels/deficiency , Calcium Channels/genetics , Calcium Signaling , Cyclic AMP/metabolism , Female , Fertilization in Vitro , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Models, Biological , Signal Transduction , Sperm Capacitation/physiology , Sperm Motility/physiology , Sperm Tail/metabolism , Spermatozoa/drug effects , Spermatozoa/ultrastructure , cdc42 GTP-Binding Protein/antagonists & inhibitors
2.
J Cell Sci ; 131(21)2018 11 08.
Article in English | MEDLINE | ID: mdl-30301778

ABSTRACT

Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In mammalian sperm, acrosomal exocytosis (denoted the acrosome reaction or AR), a special type of controlled secretion, is regulated by multiple signaling pathways and the actin cytoskeleton. However, the dynamic changes of the actin cytoskeleton in live sperm are largely not understood. Here, we used the powerful properties of SiR-actin to examine actin dynamics in live mouse sperm at the onset of the AR. By using a combination of super-resolution microscopy techniques to image sperm loaded with SiR-actin or sperm from transgenic mice containing Lifeact-EGFP, six regions containing F-actin within the sperm head were revealed. The proportion of sperm possessing these structures changed upon capacitation. By performing live-cell imaging experiments, we report that dynamic changes of F-actin during the AR occur in specific regions of the sperm head. While certain F-actin regions undergo depolymerization prior to the initiation of the AR, others remain unaltered or are lost after exocytosis occurs. Our work emphasizes the utility of live-cell nanoscopy, which will undoubtedly impact the search for mechanisms that underlie basic sperm functions.This article has an associated First Person interview with the first author of the paper.


Subject(s)
Acrosome/metabolism , Actin Cytoskeleton/metabolism , Spermatozoa/metabolism , Animals , Exocytosis , Male , Mice , Molecular Imaging
3.
J Biol Chem ; 293(24): 9435-9447, 2018 06 15.
Article in English | MEDLINE | ID: mdl-29700114

ABSTRACT

Protein kinase A (PKA) is a broad-spectrum Ser/Thr kinase involved in the regulation of several cellular activities. Thus, its precise activation relies on being localized at specific subcellular places known as discrete PKA signalosomes. A-Kinase anchoring proteins (AKAPs) form scaffolding assemblies that play a pivotal role in PKA regulation by restricting its activity to specific microdomains. Because one of the first signaling events observed during mammalian sperm capacitation is PKA activation, understanding how PKA activity is restricted in space and time is crucial to decipher the critical steps of sperm capacitation. Here, we demonstrate that the anchoring of PKA to AKAP is not only necessary but also actively regulated during sperm capacitation. However, we find that once capacitated, the release of PKA from AKAP promotes a sudden Ca2+ influx through the sperm-specific Ca2+ channel CatSper, starting a tail-to-head Ca2+ propagation that triggers the acrosome reaction. Three-dimensional super-resolution imaging confirmed a redistribution of PKA within the flagellar structure throughout the capacitation process, which depends on anchoring to AKAP. These results represent a new signaling event that involves CatSper Ca2+ channels in the acrosome reaction, sensitive to PKA stimulation upon release from AKAP.


Subject(s)
A Kinase Anchor Proteins/metabolism , Acrosome Reaction , Cyclic AMP-Dependent Protein Kinases/metabolism , Protein Interaction Maps , Sperm Capacitation , Spermatozoa/cytology , Animals , Cyclic AMP-Dependent Protein Kinases/analysis , Exocytosis , Fertilization , Male , Mice , Mice, Inbred C57BL , Signal Transduction , Spermatozoa/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL