ABSTRACT
Extraction of roots of Patrinia rupestris (Pall.) Juss. gave a new iridoid compound, 1ß,3α-diethyloxy-7-hydromethyl-4-(3-methyl-butyryloxymethyl)-cyclopenta-4(4a),7(7a)-diene[c]pyran-6-one (1), together with a known compound, (1α,4aα, 6α,7ß,7aα)-[4a,5,6,7,7a-hexahydro-6,7-dihydroxy-1-(3-methyl-1-oxobutoxy) cyclopenta[c]pyran-4,7-diyl]bis(methylene) 3-methyl-butanoic acid ester (2). The structure of 1 was characterised by HRESIMS, IR, UV, 1-D NMR and 2-D NMR methods. Compound 2 was isolated from this genus for the first time.
Subject(s)
Iridoids/isolation & purification , Patrinia/chemistry , Plant Extracts/isolation & purification , Plant Roots/chemistry , Iridoids/analysis , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Extracts/analysis , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Infrared , Spectrophotometry, UltravioletABSTRACT
A dimeric eremophilane sesquiterpene lactone with a cyclobutane ring, biliguhodgsonolide (1) and an uncommon seco-sesquiterpene derivative, (4S,5S,6R,10R)-8,9-seco-12-hydroxyeremophil-7(11)-en-14,6;12,8-diolid-9-al (2), were isolated from the roots and rhizomes of Ligularia hodgsonii Hook. Their structures, including the absolute stereochemistry, were elucidated by spectroscopic data and CD analysis. The cyclobutane ring was confirmed by single-crystal X-ray diffraction.
Subject(s)
Asteraceae/chemistry , Lactones/chemistry , Naphthalenes/chemistry , Sesquiterpenes/chemistry , Crystallography, X-Ray , Lactones/isolation & purification , Models, Molecular , Molecular Conformation , Naphthalenes/isolation & purification , Plant Roots/chemistry , Polycyclic Sesquiterpenes , Rhizome/chemistry , Sesquiterpenes/isolation & purification , StereoisomerismABSTRACT
With the development of cell culture technology in vitro, people have successfully cultivated osteoblast cells of typical characteristics from a number of animal skull, bone marrow, periosteum and bone tissues; studies have shown that these osteoblasts have good biological characteristics, can create bone tissue in different environments, can apply joint stand for the construction of tissue engineered bone, be implanted in the body to repair bone defects. In this article, the source of osteoblast, regulatory factor, composite graft and Chinese medicine research progress were reviewed.