Environmental risk substances in soil on seed germination: Chemical species, inhibition performance, and mechanisms.

Nowadays, numerous environmental risk substances in soil worldwide have exhibited serious germination inhibition of crop seeds, posing a threat to food supply and security. This review provides a comprehensive summary and discussion of the inhibitory effects of environmental risk substances on seed germination, encompassing heavy metals, microplastics, petroleum hydrocarbons, salinity, phenols, essential oil, agricultural waste, antibiotics, etc. The impacts of species, concentrations, and particle sizes of various environmental risk substances are critically investigated. Furthermore, three primary inhibition mechanisms of environmental risk substances are elucidated: hindering water absorption, inducing oxidative damage, and damaging seed cells/organelles/cell membranes. To address these negative impacts, diverse effective coping measures such as biochar/compost addition, biological remediation, seed priming, coating, and genetic modification are proposed. In brief, this study systematically analyzes the negative effects of environmental risk substances on seed germination, and provides a basis for the comprehensive understanding and future implementation of efficient treatments to address this significant challenge and ensure food security and human survival.

Aquatic life criteria of hydrothermal liquefaction wastewater via ecotoxicity test and modeling.

Wastewater resulting from hydrothermal liquefaction (HTL-AP) of biowaste is gaining attention as an emerging hazardous material. However, there is a lack of specific and systematic ecotoxicity studies on HTL-AP. This study addresses this gap by conducting acute toxicity tests on HTL-AP using typical aquatic species and integrating these results with predicted toxicity values from interspecies correlation estimation models to establish aquatic life criteria. HTL-AP exhibited significant toxicity with LC50 of 956.12-3645.4 mg/L, but demonstrated moderate toxicity compared to common freshwater pollutants like commercial microbicides, personal care products, and insect repellents. The resulting hazardous concentration for 5 % of species (HC5), the criterion maximum concentration, and the short-term water quality criteria for aquatic were 506.0, 253.0, and 168.7 mg/L, respectively. Notably, certain organisms like Misgurnus anguillicaudatus and Cipangopaludina chinensis showed high tolerance to HTL-AP, likely due to their metabolic capabilities on HTL-AP components. The significant decrease in HC5 values for some HTL-AP substances compared to pure compounds could indicate the synergistic inhibition effects among HTL-AP compositions. Furthermore, according to the established criteria, HTL-AP required significantly less diluted water (13 t) than carbendazim (1009 t) to achieve biosafety, indicating a safer release. This research establishes a preliminary water quality criterion for HTL-AP, offering a valuable reference for risk assessment and prediction in the utilization of HTL-AP within environmental contexts.

Comparative study of wound outcomes and surgical strategies: Internal fixation versus external stabilization in rib fracture patients with traumatic chest wounds.

The clinical management of traumatic chest incisions accompanied by rib fractures presents the formidable challenge. The study was carried out to compare the outcomes of auscultatory triangle internal fixation (ATIF) and external fixation (EF) in such injuries. From June 2019 to June 2022, 105 patients with multiple rib fractures participated in the cohort study in which they were divided into two groups: 53 patients underwent ATIF and 52 patients underwent EF. The incidence of surgical site infection, wound healing time, incidence of wound dehiscence, number of dressing changes, pain as measured by the visual analogue scale (VAS), duration of hospitalization, period of return to work, pulmonary complications and functionality of the upper limbs as assessed by the Disability of Arm, Shoulder, and Hand (DASH) questionnaire were among the parameters evaluated. In comparison with EF, ATIF demonstrated the decreased incidence of wound dehiscence (1.9% vs. 9.6%) (p < 0.05), surgical site infection (3.8 vs. 11.5) and wound healing time (12.3 ± 2.1 vs. 18.5 ± 3.7 days) (p < 0.05). Furthermore, during their ATIF treatment, patients required fewer changes of dressing (3.5 ± 0.8 vs. 5.7 ± 1.2) and demonstrated enhanced pain management, reduced hospital stays and expedited return to work (p < 0.05). ATIF group demonstrated enhancements in both upper limb functionality and post-operative pulmonary function (p < 0.05). The utilization of ATIF as opposed to EF for the treatment of traumatic chest wounds accompanied by rib fractures yields superior outcomes in terms of wound healing, infection reduction and restoration of pulmonary and upper limb functionality.

Van Krevelen diagrams based on machine learning visualize feedstock-product relationships in thermal conversion processes.

Feedstock properties play a crucial role in thermal conversion processes, where understanding the influence of these properties on treatment performance is essential for optimizing both feedstock selection and the overall process. In this study, a series of van Krevelen diagrams were generated to illustrate the impact of H/C and O/C ratios of feedstock on the products obtained from six commonly used thermal conversion techniques: torrefaction, hydrothermal carbonization, hydrothermal liquefaction, hydrothermal gasification, pyrolysis, and gasification. Machine learning methods were employed, utilizing data, methods, and results from corresponding studies in this field. Furthermore, the reliability of the constructed van Krevelen diagrams was analyzed to assess their dependability. The van Krevelen diagrams developed in this work systematically provide visual representations of the relationships between feedstock and products in thermal conversion processes, thereby aiding in optimizing the selection of feedstock and the choice of thermal conversion technique.

Physical properties, chemical composition, and toxicity leaching of incineration fly ash by multistage water washing.

Incineration fly ash contains a large amount of chloride, which limits the scope of its resource utilization. Water washing effectively removes chlorides and soluble substances, increasing the ability to dispose of them. The properties of incineration fly ash after multi-level water washing have been studied, providing theoretical guidance for the safe disposal of water-washed ash at all levels. Taking a practical project as an example, this paper analyzed the impact of three-stage countercurrent water washing on the physicochemical properties and toxicity leaching of incineration fly ash with different washing grades by XRD, BET, XRF, SEM, and ICP-MS. The results showed that with the improvement of washing grade, the removal rate of chloride ions was more than 86.96%. However, due to the removal of soluble substances, dioxins enriched from 98 ng-TEQ/kg of raw ash to 359 ng-TEQ/kg of tertiary washed incineration fly ash. Cr, Cu, and Zn also increased from 40.35 mg/L, 356.55 mg/L, and 3290.58 mg/L of raw ash to 136.30 mg/L, 685.75 mg/L, and 5157.88 mg/L, respectively. Pozzolanic activity had increased from 40.56% of the raw ash to 74.12% of the tertiary-washed incineration fly ash. There was no risk of excessive heavy metal leaching, and the dioxin content was lower than the raw ash in the primary washed incineration fly ash. After multi-stage water washing, incineration fly ash accumulated heavy metals, so more attention must be paid to the issue of heavy metal content in the safe disposal process.

A review of plant antipathogenic constituents: Source, activity and mechanism.

Green prevention and control of plant pathogens is a development direction of sustainable and low-carbon agriculture given the limitation of traditional chemicals. Plant-derived antipathogenic constituents (PAPCs) exhibit the advantages of being environmental benign and a broad spectrum of target pathogens over traditional chemicals. Here, we review the research advances on plant sources, chemical compositions, activities of antipathogenic constituents in the past 20 years. Reported PAPCs are classified into categories of phenols, flavonoids, terpenoids, alkaloids and antimicrobial peptides. Angiosperms, gymnosperms and some lower plants are the main plant source of detected PAPCs. The PAPCs act on pathogens through multiple pathways including destroying cell structures, blocking key composition synthesis and inhibiting cell metabolism. The development trends of PAPCs are finally prospected. This review serves as a comprehensive review on the study of plant antipathogenic constituents and a key reference for forecasting the source, characteristic and activity of PAPC.

Understand the antibacterial behavior and mechanism of hydrothermal wastewater.

Unlocking the antibacterial potential is an emerging strategy to valorizing the toxic wastewater from hydrothermal liquefaction (HTL). Here, we investigated the response and biological mechanism of antibacterial properties of HTL wastewater. Four different biowastes i.e. microalgae, cornstalk, cow manure and swine manure were used as the feedstock of HTL to create wastewater with diverse molecule spectrum, whereas ten strains i.e. five gram-positive strains and five gram-negative strains were employed to represent typical pathogenic microorganism. HTL wastewater exhibited antibacterial potential and obvious reduction on cell viability at high inclusion ratio, although the minimum inhibitory concentration (MIC) and cell response intensity varied depending on different HTL feedstocks and strain species. The decreased ATP generation and increased H2O2 accumulation in treated cells further confirmed the inhibition of HTL wastewater on the cell metabolism. The antibacterial mechanism of HTL wastewater was confirmed, including damage to biomolecules or membranes, depletion of crucial components, disruption of metabolic circuits and imbalance of creation of redox cofactor. The complex compounds in HTL wastewater were probably attributed to the multiple inhibition pathways and the relationship among those multiple pathways was speculated. The present study contributes to the mechanism analysis of complex compound mixture and bactericide characteristics of HTL wastewater.

Simultaneously Improving Ductility and Stretch Formability of Mg-3Y Sheet via High Temperature Cross-Rolling and Subsequent Short-Term Annealing.

In this work, Mg-3Y sheet was prepared by high temperature cross-rolling and subsequent short-term annealing. The effect of annealing on microstructure, texture, mechanical properties, and stretch formability of Mg-3Y sheet was primarily investigated. Micro-nano size coexistence of ß-Mg24Y5 phases can be well deformed with matrix. The as-rolled Mg-3Y sheet exhibited a homogeneous deformation microstructure consisting of deformed grains with extensive kink bands and dispersed ß-Mg24Y5 phases. A double peak texture character appeared in as-rolled Mg-3Y sheet with a split of the texture peaks of about ±20° tilted to rolling direction. After annealing, the as-annealed Mg-3Y sheet presented complete static recrystallized (SRXed) microstructure consisting of uniform equiaxed grains. The texture orientation distribution was more dispersed and a weakened multiple-peak texture orientation distribution appeared. In addition, the maximum intensity of basal plane decreased from 5.2 to 3.1. The change of texture character was attributed to static recrystallization (SRX) induced by kink bands and grain boundaries. The as-annealed Mg-3Y sheet with high Schmid factor (SF) for basal slip, prismatic slip, pyramidal slip, and pyramidal slip exhibited high ductility (~25.6%). Simultaneously, enhanced activity of basal slip and randomized grain orientation played a significant role in decreasing anisotropy for the as-annealed Mg-3Y sheet, which contributed to the formation of high stretch formability (~6.2 mm) at room temperature.

A Cohort Study on the Comparison of Complications, Short-Term Efficacy, and Quality of Life between Thoracoscopic Surgery and Traditional Surgery in the Treatment of Rib Fractures.

Objective: A case-control study was conducted, to assess the complications, short-term effectiveness, and quality of life of video-assisted thoracoscopic surgery with conventional surgery in the treatment of rib fractures. Methods: From February 2018 to April 2021, 100 patients with rib fractures who required surgical treatment at the hospital were selected. Patients were randomly divided into control and study groups. The study group received thoracoscopy-assisted rib internal fixation, and the control group received traditional open reduction and internal fixation for rib fractures. The treatment effect, postoperative complication rate, surgery-related indicators, stress response, blood gas indicators, VAS (visual analog scale) pain score, and SF-36 quality of life score were compared between the two groups. Results: The total effective rate of the study group was higher than that of the control group, and the difference was statistically significant (P < 0.05). The postoperative complications in the study group were significantly lower than those in the control group (χ 2-5.317; P < 0.05), but there was no significant difference in hospitalization costs between the two groups (P > 0.05). The operation time, intraoperative blood loss, incision length, drainage tube placement time, postoperative activity time, and hospital stay in the study group were significantly lower than those in the control group. The SF-36 score and VAS score in the study group were higher than those in the control group (P < 0.05). Compared with the two groups after the operation, the levels of PaO2, SaO2, and PaO2/FiO2 in the study group were significantly higher than those in the control group (P < 0.05). Before surgery, there was no significant difference in stress response indicators such as cortisol, blood sugar, and C-reactive protein between the two groups (P > 0.05), but there was no significant difference in stress response indicators after surgery (P > 0.05). Cortisol, blood sugar, C-reactive protein, and other indicators were increased in both groups, but compared with the control group, the study group had decreased postoperative cortisol, blood sugar, C-reactive protein, and other stress response indicators (P < 0.05). Conclusion: There is a significant difference between thoracoscopic surgery and traditional surgery in the treatment of rib fractures. The probability of postoperative complications of thoracoscopic surgery is lower, and the operation time, intraoperative blood loss, and incision length are better. The pain of patients before and after the operation is significantly reduced, the quality of life is improved greatly, and the stress response is weak.

Construct a novel anti-bacteria pool from hydrothermal liquefaction aqueous family.

Hydrothermal liquefaction aqueous phase (HTL-AP) is complex and toxic, which severely hinders the scale-up of HTL technology. Distinguished from degrading organics and extracting chemical energy or nutrients from HTL-AP via biological fermentation or algae cultivation, here, we propose an innovative strategy to valorize the HTL-AP as a powerful anti-bacterial pool. Six model ingredients, i.e. lipids, cellulose, xylan, lignin, protein and the mixture were employed, to obtain a thirty-HTL-AP pool for characteristics database construction. We found that the xylan group at 230 °C on Escherichia coli (E. coli) and at 200 °C on Staphylococcus aureus (S. aureus) exhibited the highest anti-bacterial activities via plate experiments, nearly equal to 100 µg/ml streptomycin which far exceeded the working concentration of streptomycin (10-50 µg/ml). The liquid cultivation studies further revealed HTL-APs from the mixture feedstock, protein, real biomass microalgae and cornstalk had more stable anti-bacterial activities as chemically stable substances. Interestingly, the Gram-positive strain S. aureus was more susceptible than the Gram-negative E. coli on the HTL-APs, probably owing to the outer selectively permeable membrane difference and the strong reducibility and acidity of HTL-APs. This study provides a new vision to seek the anti-bacterial potential of HTL aqueous, supporting further investigations on its molecular mechanism and new bactericide development.

The relationship between Beclin 1 expression and lymph node metastasis in non-small cell lung cancer / 中国胸心血管外科临床杂志

@#Objective    To explore the relationship between Beclin 1 level and lymph node metastasis in patients with non-small cell lung cancer. Method    A total of 204 surgical specimens of patients with non-small cell lung cancer from September 2011 to September 2016 were collected in our hospital. There were 116 males and 88 females . Beclin 1 levels were detected by Western blotting. There were 116 males and 88 females at average age of 55.3±11.2 years. The patients were divided into three groups including a group N0 (no lymph node metastasis), a group N1(intralobar and interlobar lymph node metastases, and no mediastinal lymph node metastasis), and a group N2 (mediastinal lymph node metastasis). The differences of Beclin 1 levels in tumor tissues and lymph nodes of patients with N0, N1 and N2 were statistically analyzed. Results    Among 204 patients of lung cancer, 36 patients were squamous cell carcinoma and 168 patients were adenocarcinoma. The levels of Beclin 1 in tumor tissues of N0, N1 and N2 groups decreased gradually with a statistical difference (P<0.05). In the three groups, the levels of Beclin 1 in the lung hilum and intrapulmonary lymph nodes (N1 Beclin 1) of N1 and N2 groups were less than that of N0 group with a statistical difference (P<0.01). In the three groups, the level of Beclin 1 in the mediastinal lymph nodes (N2 Beclin 1) of N2 group was less than that of the N0 and N1 groups with a statistical difference (P<0.01). In the N1 group, the level of N1 Beclin 1 was less than that of N2 group (P<0.01). In the N2 group, though the level of N1 Beclin 1 was less than N2 Beclin 1, there was no statistical difference (P>0.05). Conclusion    Beclin 1 level can be used as a reference index to judge the benign and malignant lung masses, and lymph node Beclin 1 level can be used as an important reference index to help determine whether there is lymph node metastasis in lung cancer.

Identifying Keystone Species in the Microbial Community Based on Cross- Sectional Data.

BACKGROUND: In microbial communities, the keystone species have a greater impact on the performance and dynamics of ecosystem than that of other species, in which we can see from the results that losing gut microbiome causes some specific diseases. A number of ongoing studies aim at identifying links between microbial community structure and human diseases. METHOD: In this paper, we have introduced a valid keystone species identification method, in which a new Spread Intensity (SI) algorithm is used. Because the accuracies of current keystone species identification algorithms are difficult to evaluate for the high diversity and uncultivated status of microbial communities, we simulated cross-sectional data of microbial communities with known interactions and set up standard keystoneness rankings using Generalized Lotka-Volterra (GLV) model. Subsequently, we compared the SI algorithm with existing methods by using simulated data and obtained an obvious better performance of SI algorithm than other methods. Also, we applied this method to gut microbiota datasets and identified some microbes having the potential association with body weight. We first assembled three correlation metrics to calculate the interspecies correlation. Then we applied network deconvolution to remove indirect correlations. Finally, we used Molecular Ecological Network Analysis (MENA) to construct the co-occurrence network. According to experimental results, SI algorithm has an excellent performance in identifying highly correlated species in gut microbiome to body weight. RESULT: This result provides an effective indicator for modulating gut microbiota and thus enables the gene therapy and other gene-level treatments for losing-weight and other gut-associated diseases.

Biodegradable Magnesium Alloys Developed as Bone Repair Materials: A Review.

Bone repair materials are rapidly becoming a hot topic in the field of biomedical materials due to being an important means of repairing human bony deficiencies and replacing hard tissue. Magnesium (Mg) alloys are potentially biocompatible, osteoconductive, and biodegradable metallic materials that can be used in bone repair due to their in situ degradation in the body, mechanical properties similar to those of bones, and ability to positively stimulate the formation of new bones. However, rapid degradation of these materials in physiological environments may lead to gas cavities, hemolysis, and osteolysis and thus, hinder their clinical orthopedic applications. This paper reviews recent work on the use of Mg alloy implants in bone repair. Research to date on alloy design, surface modification, and biological performance of Mg alloys is comprehensively summarized. Future challenges for and developments in biomedical Mg alloys for use in bone repair are also discussed.

A silica sands-based method for faithful analysis of microbial communities and DNA isolation from a wide range of species.

A silica sands-based method has been developed to isolate high quality genomic DNAs from cells of animals, plants and microorganisms, such as Hemisalanx prognathus, Spinacia oleracea, Pichia pastoris, Bacillus licheniformis and Escherichia coli. To the best of our knowledge, no DNA isolation method has so wide application until now. In addition, this method and a commercially available kit were compared in analysis of microbial communities using high-throughput 16s rDNA sequencing. As a result, the silica sands-based method was found to be even more efficient in isolating genomic DNA from gram-positive bacteria than the kit, indicating that it would become a very valuable choice to faithfully reflect the composition of microbial communities.

A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis.

The plant homeodomain (PHD) finger-containing proteins have been implicated in many human diseases including cancer. In this study, we found that PHF14, a newly identified PHD finger protein, is highly expressed in lung cancer. The high expression level of PHF14 was associated with adenocarcinoma and poor survival in lung cancer patients. Knocking down PHF14 suppressed cancer cell growth and carcinogenesis, while over-expressing PHF14 promoted cell proliferation. During cell division, PHF14 directly bound to and co-localized with KIF4A (a nuclear motor protein involved in lung carcinogenesis) to form a functional complex. Similarly to the effect of KIF4A depletion, silencing PHF14 in several cell lines caused cell mitotic defects, prolonged M phase, and inhibited cell proliferation. What's more, these two proteins had a synergistic effect on cell proliferation and were significantly co-overexpressed in lung cancer tissues. Our data provide new insights into the biological significance of PHD finger proteins and imply that PHF14 may be a potential biomarker for lung cancer.

Correlation between anastomotic angle and postoperative anastomotic stricture in the surgery of esophageal carcionma: A randomized controlled trial / 中国胸心血管外科临床杂志

@#Objective    To investigate the correlation between end-to-side anastomotic angle and postoperative anastomotic stricture in the surgery of esophageal carcinoma. Methods    From January 2011 to June 2015, 130 patients with middle/lower esophageal carcinoma or gastric cardia cancer underwent operations in Shanghai Pudong Hospital and Lishui Central Hospital, Zhejiang Province. Depending on the end-to-side anastomotic angle, they were randomly divided into two groups (n=65 in each): a 0 degree group (49 males and 16 females, aged 64.5±8.3 years) and a 45 degrees group (52 males, 13 females, aged 61.7±9.1 years). Stooler degree grading was adopted to evaluate the anastomotic stricture in each group 6 months postoperatively. Results    There were two patients with anastomotic fistula in each group (P>0.05). Pathology showed squamous carcinoma in 116 patients and adenocarcinoma in 14 patients. The postoperative esophageal stricture in the 45 degrees group was significantly less than that in the 0 degree group. There was no statistical difference in the duration of chest tube (5.9±6.7 d vs. 5.8±6.8 d) and recovery of intestinal peristalsis (2.6±0.8 d vs. 2.6±0.7 d) between the 45 degrees group and the 0 degree group. Conclusion    Esophagogastric anastomotic angle is related to the formation of postoperative anastomotic stricture. Oblique anastomosis with 45 degrees is helpful to decrease the severity of stricture.

Curcumin inhibits cell proliferation and induces apoptosis of human non-small cell lung cancer cells through the upregulation of miR-192-5p and suppression of PI3K/Akt signaling pathway.

Curcumin is the main active ingredient extracted from the traditional Chinese medicine, turmeric, which acts against non-small cell lung cancer cell (NSCLC), lowers blood pressure, is anti-inflammatory, choleretic, and exerts anti­oxidant effects, without any obvious toxicity in the long term. The aim of the present study was to investigate whether the anticancer effect of curcumin inhibited cell proliferation and induced apoptosis of human NSCLC through the upregulation of microRNA­192-5p (miR-192-5p) and suppression of the PI3K/Akt signaling pathway. In the present study, treatment with curcumin inhibited cell proliferation, induced cell apoptosis and increased the caspase-3 activity of A549 cells. The results also showed that, miR-192-5p relative expression of NCL-H460 cells was relatively lower than that of A549 cells, which was higher, with that of BEAS-2E cells being the highest. miR-192-5p mimics suppressed cell proliferation and increased cell apoptosis of A549 cells. However, anti-miR-192-5p mimics increased cell proliferation and inhibited cell apoptosis of A549 cells. Curcumin treatment effectively increased the relative miR­192-5p expression and suppressed the PI3K/Akt signaling pathway. miR-192-5p mimics enhanced the effect of curcumin on cell viability and apoptosis and suppressed the PI3K/Akt signaling pathway in A549 cells. Anti-miR-192-5p mimics reversed the effect of curcumin on A549 cells and PI3K/Akt expression. Collectively, our findings suggested that curcumin inhibited cell proliferation and induced apoptosis of human non-small cell lung cancer cells through the upregulation of miR-192-5p and suppression of the PI3K/Akt signaling pathway.

The complicated substrates enhance the microbial diversity and zinc leaching efficiency in sphalerite bioleaching system.

This study used an artificial enrichment microbial consortium to examine the effects of different substrate conditions on microbial diversity, composition, and function (e.g., zinc leaching efficiency) through adding pyrite (SP group), chalcopyrite (SC group), or both (SPC group) in sphalerite bioleaching systems. 16S rRNA gene sequencing analysis showed that microbial community structures and compositions dramatically changed with additions of pyrite or chalcopyrite during the sphalerite bioleaching process. Shannon diversity index showed a significantly increase in the SP (1.460), SC (1.476), and SPC (1.341) groups compared with control (sphalerite group, 0.624) on day 30, meanwhile, zinc leaching efficiencies were enhanced by about 13.4, 2.9, and 13.2%, respectively. Also, additions of pyrite or chalcopyrite could increase electric potential (ORP) and the concentrations of Fe3+ and H+, which were the main factors shaping microbial community structures by Mantel test analysis. Linear regression analysis showed that ORP, Fe3+ concentration, and pH were significantly correlated to zinc leaching efficiency and microbial diversity. In addition, we found that leaching efficiency showed a positive and significant relationship with microbial diversity. In conclusion, our results showed that the complicated substrates could significantly enhance microbial diversity and activity of function.

Docetaxel-loaded chitosan microspheres as a lung targeted drug delivery system: in vitro and in vivo evaluation.

The aim of this study was to prepare docetaxel-loaded chitosan microspheres and to evaluate their in vitro and in vivo characteristics. Glutaraldehyde crosslinked microspheres were prepared using a water-in-oil emulsification method, and characterized in terms of the morphological examination, particle size distribution, encapsulation ratio, drug-loading coefficient and in vitro release. Pharmacokinetics and biodistribution studies were used to evaluate that microspheres have more advantage than the conventional formulations. The emulsion crosslinking method was simple to prepare microspheres and easy to scale up. The formed microspheres were spherical in shape, with a smooth surface and the size was uniform (9.6 ± 0.8 µm); the encapsulation efficiency and drug loading of prepared microspheres were 88.1% ± 3.5% and 18.7% ± 1.2%, respectively. In vitro release indicated that the DTX microspheres had a well-sustained release efficacy and in vivo studies showed that the microspheres were found to release the drug to a maximum extent in the target tissue (lung). The prepared microspheres were found to possess suitable physico-chemical properties and the particle size range. The sustained release of DTX from microspheres revealed its applicability as drug delivery system to minimize the exposure of healthy tissues while increasing the accumulation of therapeutic drug in target sites.