The antioxidant status and biochemical parameters in kid goats naturally infected with peste des petits ruminants virus

Background: Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small ruminants. The disease is high economical importance because of the high mortality rate. Oxidative stress is an active field of research in small ruminant medicine and has been implicated in numerous disease processes including sepsis, mastitis, acidosis, enteritis, pneumonia, respiratory, and joint diseases. Compared to human medicine, only a limited number of conditions have been investigated in regard to the effects of oxidative stress in small ruminants. The aim of this study was to determined and compared the oxidative status and some biochemical parameters in kid goats with PPR. Materials, Methods & Results: The study was performed on 15 healthy hair of kid goats (control group) and 15 kids naturally infected with Peste des Petits Ruminants (PPR). Competitive enzyme linked immunosorbent assay (C-ELISA) was used for serological detection of PPRV specific antibodies, and a reverse transcription polymerase chain reaction (RT-PCR) was performed for the detection of PPR virus. Concentrations of plasma biochemical parameters were analysed by a clinical chemistry analyser, and blood biochemical indices determined, including total protein, albumin, alkaline phosphatase (ALP), aspartate amino transferase (AST), γ- glutamyl transferase (GGT), lactate dehydrogenase (LDH), glucose, very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL). The plasma CAT activity, plasma GSHPx activity and plasma lipid peroxidation level was measured according to the specific methods. Besides, vitamin C values were colorimetrically determined using a phosphotungustic method acid method and vitamin E values were determined spectrophotometrically method.[...](AU)

The antioxidant status and biochemical parameters in kid goats naturally infected with peste des petits ruminants virus

Background: Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small ruminants. The disease is high economical importance because of the high mortality rate. Oxidative stress is an active field of research in small ruminant medicine and has been implicated in numerous disease processes including sepsis, mastitis, acidosis, enteritis, pneumonia, respiratory, and joint diseases. Compared to human medicine, only a limited number of conditions have been investigated in regard to the effects of oxidative stress in small ruminants. The aim of this study was to determined and compared the oxidative status and some biochemical parameters in kid goats with PPR. Materials, Methods & Results: The study was performed on 15 healthy hair of kid goats (control group) and 15 kids naturally infected with Peste des Petits Ruminants (PPR). Competitive enzyme linked immunosorbent assay (C-ELISA) was used for serological detection of PPRV specific antibodies, and a reverse transcription polymerase chain reaction (RT-PCR) was performed for the detection of PPR virus. Concentrations of plasma biochemical parameters were analysed by a clinical chemistry analyser, and blood biochemical indices determined, including total protein, albumin, alkaline phosphatase (ALP), aspartate amino transferase (AST), γ- glutamyl transferase (GGT), lactate dehydrogenase (LDH), glucose, very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL). The plasma CAT activity, plasma GSHPx activity and plasma lipid peroxidation level was measured according to the specific methods. Besides, vitamin C values were colorimetrically determined using a phosphotungustic method acid method and vitamin E values were determined spectrophotometrically method.[...]