ABSTRACT
AIM: To examine human beta-defensin-3 (hBD-3) expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H. pylori infection for better understanding the innate immune response to H. pylori. METHODS: We used reverse transcription-polymerase chain reactions and immunohistochemistry to examine hBD-3 expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H. pylori. Effects of hBD-3 against H. pylori were also evaluated. RESULTS: The mean mRNA expression of hBD-3 in H. pylori-positive specimens was significantly higher than that in H pylori-negative specimens (P = 0.0002, Mann-Whitney). In addition, unlike uninfected samples, 8 of 15 (53.33%) infected mucosal samples expressed hBD-3 protein. H. pylori dose-dependently induced mRNA expression of hBD-3 in MKN45 cells, an effect inhibited by adding anti-toll-like receptor (TLR)-4 antibody. HBD-3 protein completely inhibited H. pylori growth. CONCLUSION: Our results suggest that like hBD-2, hBD-3 may be involved in the pathophysiology of H. pylori-induced gastritis.
Subject(s)
Gastric Mucosa/metabolism , Gastritis/metabolism , Helicobacter Infections/metabolism , beta-Defensins/metabolism , Antibodies, Anti-Idiotypic/immunology , Antibodies, Anti-Idiotypic/pharmacology , Cell Line, Tumor , Gastric Mucosa/microbiology , Gastric Mucosa/pathology , Gastritis/genetics , Gastritis/microbiology , Gastritis/physiopathology , Gene Expression Regulation , Gene Expression Regulation, Neoplastic , Helicobacter Infections/genetics , Helicobacter Infections/physiopathology , Helicobacter pylori/drug effects , Helicobacter pylori/pathogenicity , Humans , RNA, Messenger/genetics , RNA, Messenger/metabolism , Stomach Neoplasms/genetics , Stomach Neoplasms/metabolism , Toll-Like Receptor 4/immunology , beta-Defensins/genetics , beta-Defensins/pharmacologyABSTRACT
BACKGROUND: Ischemia-reperfusion (I/R) injury occurs in various situations, including transplantation, trauma, and shock. We previously reported that the synthetic beta-SQDG (18:0), which was derived from sulfoquinovosyl diacylglycerol of the sea urchin, possessed immunosuppressive effects, such as inhibition of T-cell responses in human allogenic human mixed lymphocyte reactions (MLR) and skin allograft survival in rats. beta-SQAG9 was synthesized from beta-SQDG (18:0) to improve structural stability in aqueous solution with the same biological activities to bind to CD62L (L-selectin) and CD62P (P-selectin) in vitro. We hypothesized that beta-SQAG9 might attenuate leukocyte rolling on the endothelium and neutrophil infiltration in which L-selectin and P-selectin are key molecules. We investigated the protective effect of beta-SQAG9 against hepatic I/R injury. METHODS: Male Lewis rats were divided into 6 groups: sham, control, and treatment. Rats in the control, and the treatment groups were subjected to hepatic ischemia for 30 minutes. They were injected with PBS or beta-SQAG9 at doses of 5, 10, 25, and 50 mg/kg into the penile vein immediately before reperfusion. To assess the damage to the hepatic parenchyma, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) were measured and histological evaluation was performed at 6 hours after reperfusion. RESULTS: In the group treated with beta-SQAG9 at a dose of 10 mg/kg, AST, ALT, and LDH were significantly reduced, and the amount of neutrophil infiltration also was significantly reduced. CONCLUSIONS: Our data suggest that SQAG-9 (10 mg/kg) reduces the warm hepatic I/R injury.
Subject(s)
Glycolipids/therapeutic use , Liver Circulation , Reperfusion Injury/prevention & control , Animals , Glycolipids/isolation & purification , Immunosuppressive Agents/isolation & purification , Immunosuppressive Agents/therapeutic use , Liposomes , Liver/drug effects , Liver/pathology , Male , Necrosis , Neutrophils/pathology , Rats , Rats, Inbred Lew , Sea UrchinsABSTRACT
BACKGROUND: In hepatic surgery and liver transplantation, ischemia-reperfusion (I/R) is an unavoidable process, and protection against hepatic I/R injury is a major unresolved problem. In this study, we investigated whether 3-O-(6-deoxy-6-sulfono-beta-D-glucopyranosyl)-1,2-di-O-acylglycerol bound to saturated C18 fatty acids (beta-SQAG9), which was derived from sea urchin intestines, could reduce this injury. This agent was recently reported to have immunosuppressive effects in allogeneic rat skin grafts. MATERIALS & METHODS: Male Lewis rats were divided into two experimental groups. Group 1 rats were injected with SQAG9 (50 mg/kg) into the penile vein 15 minutes before the induction of ischemia and into the portal vein just reperfusion. The same amounts of normal saline were injected into rats in the control group (group 2). Each experimental groups included six rats. Seventy percent hepatic ischemia (20 minutes) was induced by occluding the blood vessels and bile duct with a vascular clamp. For examination of hepatic function, serum levels of aspartate aminotransferase, (AST) alanine transaminase (ALT), and lactic dehydrogenase (LDH) were measured. In addition, histological examination was also assessed. RESULTS: Three hours after reperfusion, the mean plasma concentration of AST, ALT, LDH in group 1 was suppressed compared with group 2. Six hours after reperfusion, the hepatic damage in group 1 was mild in comparison with that in group 2. CONCLUSIONS: Our data demonstrated that SQAG-9 reduced the warm hepatic I/R injury.
Subject(s)
Diglycerides/pharmacology , Glycolipids/pharmacology , Liver Circulation/drug effects , Liver , Reperfusion Injury/prevention & control , Animals , Liver/blood supply , Liver/drug effects , Liver/pathology , Liver Function Tests , Male , Rats , Rats, Inbred Lew , Sea Urchins/metabolismABSTRACT
BACKGROUND: Intra-operative monitoring of the position of the central sulcus (CS) is indispensable to properly treat a peri-motor cortex lesion. Noninvasive preoperative studies for identification of CS are also needed for choosing the optimal operative procedure. Magneto-encephalography (MEG) has recently been introduced for non-invasive preoperative investigation and cortical functional mapping. METHODS: Stereotactic mapping of functional CS anatomy was performed preoperatively on 13 subjects using somatosensory evoked fields with MRI-linked whole head MEG system. All subjects had a left sided peri-motor cortex lesion with diagnoses including the following conditions: three arteriovenous malformations (AVM), seven gliomas, three meningiomas. FINDINGS: Functional CS in supratentorial brain tumor and parietal AVM cases corresponded with anatomical CS identified by MRI. But the AVM cases in whom the nidus was situated within the peri-motor cortex showed discrepancies between anatomical CS and functional CS. INTERPRETATION: Careful consideration of the operative procedure combined with non-invasive cortical functional mapping is needed to optimally treat AVM and congenital brain lesions situated in the anatomical peri-motor cortex.
Subject(s)
Brain Mapping/methods , Intracranial Arteriovenous Malformations/complications , Intracranial Arteriovenous Malformations/surgery , Motor Cortex/anatomy & histology , Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Magnetic Resonance Imaging , Magnetoencephalography , Male , Middle Aged , Monitoring, Intraoperative , Motor Cortex/surgery , Stereotaxic TechniquesSubject(s)
Angiotensin Receptor Antagonists , Benzimidazoles/therapeutic use , Biphenyl Compounds/therapeutic use , Hepatocyte Growth Factor/physiology , Liver Circulation/physiology , Liver , Reperfusion Injury/prevention & control , Tetrazoles , Transforming Growth Factor beta/physiology , Animals , Enzyme-Linked Immunosorbent Assay , Gene Expression Regulation/drug effects , Hepatocyte Growth Factor/genetics , Liver/pathology , Liver Circulation/drug effects , Male , Rats , Rats, Inbred Strains , Receptor, Angiotensin, Type 1 , Transforming Growth Factor beta/geneticsABSTRACT
The objective of this study was to understand more of the innate immune response to Helicobacter pylori by determining the expression of human beta-defensin-2 (hBD-2) in various gastric mucosal tissues and MKN45 gastric cancer cells with or without H. pylori. Semi-quantitative TaqMan RT-PCR and immunohistochemistry were carried out. The antimicrobial effects of a transfected hBD-2 gene against H. pylori were also evaluated. The results showed that hBD-2 was expressed in inflamed gastric mucosal tissues with H. pylori infection, but not in the absence of H. pylori infection. Expression was also detected in gastric cancers in patients with H. pylori infection. Expression was induced in the MKN45 gastric cancer cell line by H. pylori in a manner dependent on the abundance of bacteria. hBD-2-transfected 3T3J2-1 cells secreted hBD-2 protein into the culture medium and this protein inhibited growth of H. pylori completely. The results suggest that hBD-2 may be involved in the pathophysiology of H. pylori-induced gastritis.
Subject(s)
Gastric Mucosa/microbiology , Gene Expression Regulation , Helicobacter Infections/genetics , Helicobacter Infections/immunology , Helicobacter pylori/immunology , beta-Defensins/genetics , beta-Defensins/immunology , Adult , Colony Count, Microbial , Enterococcus faecalis/physiology , Escherichia coli/physiology , Gastric Mucosa/chemistry , Gastric Mucosa/immunology , Gastritis/immunology , Gastritis/microbiology , Helicobacter pylori/isolation & purification , Helicobacter pylori/physiology , Humans , Immunity, Innate , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Salmonella typhimurium/physiology , Staphylococcus aureus/physiology , Stomach Neoplasms/immunology , Stomach Neoplasms/microbiology , Transfection , Tumor Cells, Cultured , beta-Defensins/analysisABSTRACT
It has been clearly shown that autoimmune diseases can be treated by apheresis by eliminating immune complexes, however, the effects of therapeutic apheresis are not limited to immune disorders. Almost all diseases are associated with immune systems. Immune systems can be regulated by advanced techniques of apheresis, including immunoadsorption and immunocytapheresis, removing immune effector molecules and various immune-associated cells selectively. Therefore, apheresis can be used as a nondrug treatment for many diseases. In addition, disease-associated proteins that cause disease or are produced in the course of diseases and accumulate in the body could be eliminated selectively by apheresis using the extremely powerful ability of the immune system to recognize polypeptide structures specifically and distinguish miniscale differences among molecules. In this article, we discuss the current status of treatment of immune diseases by apheresis and possible treatment approach of a variety of diseases by apheresis based on immune reactions.
Subject(s)
Blood Component Removal , Immune System Diseases/therapy , Autoimmune Diseases/therapy , B-Lymphocytes/physiology , Cytokines/immunology , Extracorporeal Circulation , Humans , Immunosorbent Techniques , Neoplasms/immunology , T-Lymphocytes/immunology , Transplantation ImmunologyABSTRACT
AIMS: The aim of this study was to analyse genotypes for clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA), including hetero-vancomycin-resistant Staph. aureus (VRSA), at a Japanese university hospital. METHODS AND RESULTS: Seventy-eight clinical isolates of MRSA were analysed by arbitrarily primed-polymerase chain reaction (AP-PCR) using ERIC2 primer and by pulse-field gel electrophoresis (PFGE) following SmaI digestion. Analyses of the nine genotypes and 28 subtypes defined by PFGE, and of the three genotypes and 22 subtypes defined by AP-PCR, both facilitated epidemiological tracing. Used in combination, AP-PCR and PFGE provided more precise classification than the use of a single genotyping method. The six hetero-VRSA isolates were classified into four genotypes defined by the combination of both methods, but these genotypes contained non-VRSA isolates. CONCLUSIONS: The results suggest that both PFGE and AP-PCR are useful in discriminating MRSA, but not hetero-VRSA, isolates for epidemiological analysis. SIGNIFICANCE AND IMPACT OF THE STUDY: Combining the results of PFGE with the results of AP-PCR can provide more detail differentiation of MRSA and hetero-MRSA isolates than either method alone.
Subject(s)
Electrophoresis, Gel, Pulsed-Field/methods , Methicillin Resistance , Polymerase Chain Reaction/methods , Staphylococcus aureus/classification , DNA, Bacterial/analysis , Genome, Bacterial , Genotype , Humans , Japan/epidemiology , Staphylococcus aureus/drug effects , Staphylococcus aureus/genetics , Vancomycin ResistanceABSTRACT
Thirty Japanese clinical isolates of Mycobacterium tuberculosis were analysed by pyrazinamide susceptibility testing and pyrazinamidase assay, as well as polymerase chain reaction for single-strand conformational polymorphism and direct sequencing of the gene encoding pyrazinamidase (pncA). All sensitive isolates showed pyrazinamidase activity and a wild-type pncA gene, but three resistant isolates had pncA gene mutations and lacked pyrazinamidase activity. The latter isolates showed a minimum inhibitory concentration of at least 100 mg/l by the 7H10 agar proportion method and 400 mg/l by the 7H9 liquid medium method. Isolate 28 showed T-to-C change at position 11, leading to Leu4 --> Ser substitution; isolate 29 had an 8-bp deletion from position 382; and isolate 30 had A-to-C change at position 29, leading to Gln10 --> Pro substitution. The deletion has not been described previously. This is the first demonstration of pncA gene mutations in PZA-resistant M. tuberculosis strains isolated from Japanese patients.
Subject(s)
Amidohydrolases/genetics , Antitubercular Agents/pharmacology , DNA, Bacterial/genetics , Mycobacterium tuberculosis/drug effects , Mycobacterium tuberculosis/genetics , Pyrazinamide/pharmacology , DNA Mutational Analysis , DNA Primers , Dose-Response Relationship, Drug , Drug Resistance/genetics , Humans , Japan , Polymerase Chain ReactionSubject(s)
Anti-Bacterial Agents/pharmacology , Electrophoresis, Gel, Pulsed-Field/methods , Methicillin Resistance/genetics , Staphylococcus aureus/genetics , Vancomycin/pharmacology , Genes, Bacterial , Genotype , Humans , Microbial Sensitivity Tests , Phenotype , Staphylococcus aureus/classification , Staphylococcus aureus/drug effectsABSTRACT
Telomerase adds hexameric repeats of 5'-TTAGGG-3' termed telomeres to ends of chromosomal DNA. This enzyme has been implicated in cellular immortalization and cellular senescence. Recently, a number of relevant genes have been cloned, including these encoding three major components of human telomerase: human telomerase RNA component (hTR), human telomerase reverse transcriptase (hTERT), and telomerase-associated protein-1 (TEP1). Also important are genes encoding human telomeric-repeat binding factor protein (TRF) 1 and 2. To clarify mechanisms regulating telomerase activity, we studied telomerase activity, the telomeric restriction fragment (TRF) length and gene expression of these telomerase components and the telomeric-repeat binding factor proteins in sequential observation following X-irradiation of cultured pancreatic cancer cells. We previously reported that PANC-1 cells are better able to tolerate thermal stress, antineoplastic drugs, and exposure to tumour necrosis factor than MIAPaCa-2 cells. MIAPaCa-2 and PANC-1 cells were exposed to X-irradiation, their telomerase activity was increased at 2 days and then decreased gradually. Of the three telomerase components, only hTERT mRNA expression showed parallel changes. TRF length was stable just before and after X-irradiation. Among binding factor proteins, TRF1 mRNA showed reciprocal changes possibly directed toward maintaining a stable telomere length. In this study, our results demonstrate that not only hTERT but also TRF1 are important regulator of telomerase activity.
Subject(s)
DNA-Binding Proteins/metabolism , Telomerase/metabolism , Cell Count , Gene Expression Regulation , Humans , Pancreatic Neoplasms/metabolism , RNA, Messenger/metabolism , Telomeric Repeat Binding Protein 1 , Tumor Cells, Cultured/cytology , Tumor Cells, Cultured/radiation effectsABSTRACT
A 20-year-old female experienced temporary unintentional mirror writing associated with low perfusion of the bilateral anterior cerebral arteries. When she was 17 years old, she had developed multiple idiopathic intracerebral hemorrhages and right hemiparesis. At the age of 20, she had a generalized convulsion for which she was transferred to our department. Computed tomography (CT) and magnetic resonance images of the brain were obtained, but no fresh abnormal lesion could be detected. The following day, after she had recovered from postictal symptoms, she wrote mirror image words, and her mirror writing then gradually improved within one week. Single photon emission CT showed low perfusion of both anterior cerebral arteries. We concluded that bilateral vascular insufficiency to the supplementary motor areas and corpus callosum caused mirror writing in this case.
Subject(s)
Anterior Cerebral Artery , Cerebral Arterial Diseases/physiopathology , Cerebrovascular Circulation , Dominance, Cerebral , Adult , Brain/diagnostic imaging , Brain/pathology , Cerebral Arterial Diseases/diagnosis , Female , Humans , Magnetic Resonance Imaging , Tomography, Emission-Computed, Single-PhotonABSTRACT
BACKGROUND: The significance of serum concentrations of tumor necrosis factor-alpha (TNF-alpha) in the pathogenesis of inflammatory bowel disease (IBD) is uncertain. We measured TNF-alpha in serum from IBD patients by immuno-PCR to analyze the relationship between TNF-alpha and pathophysiologic state in IBD. METHODS: Serum samples were collected from 54 healthy blood donors, 29 patients with ulcerative colitis (UC; 46 samples), and 7 patients with Crohn disease (CD; 8 samples). DNA label was generated by PCR amplification using biotinylated primer and was bound with streptavidin to biotinylated third antibody. TNF-alpha sandwiched by antibodies was detected by PCR amplification of the DNA label. RESULTS: TNF-alpha could be measured in all samples. The median serum concentration in IBD patients overall was approximately 390-fold higher than in healthy donors (median increase, 380-fold for UC, 640-fold for CD). The median serum TNF-alpha concentration was 1.7-fold higher in the active stage of UC than in the inactive stage (P <0.05), and this difference could be detected in individual patients. CONCLUSIONS: Sensitive measurement of serum TNF-alpha could provide an important pathophysiologic marker for the presence and activity of IBD.
Subject(s)
Inflammatory Bowel Diseases/blood , Tumor Necrosis Factor-alpha/analysis , Adult , Aged , Antibodies , Biomarkers/blood , Biotinylation , Colitis, Ulcerative/blood , Colitis, Ulcerative/pathology , Crohn Disease/blood , Crohn Disease/pathology , Enzyme-Linked Immunosorbent Assay , Female , Humans , Inflammatory Bowel Diseases/pathology , Male , Middle Aged , Polymerase Chain Reaction/methods , Sensitivity and Specificity , StreptavidinABSTRACT
Telomerase activation is a characteristic of immortalized tumor cells but not of normal cells. Telomerase activity has been detected in approximately 85% of malignant tumors, and assaying for telomerase activity is thought to be useful for diagnosing cancer. Three telomerase-associated molecules [human telomerase RNA component (hTR), telomerase-associated protein (TEP1), and human telomerase reverse transcriptase (hTERT)] have been cloned. We semiquantitatively measured telomerase activity and the expression of these genes in cancerous and noncancerous regions of gastric cancer patients. We also investigated whether the expression of these genes correlated with telomerase activity. Telomerase activity in cancerous regions was significantly higher than in noncancerous regions, but there was no correlation between telomerase activity and the expression of these genes. Furthermore, no clear difference was observed between cancerous and noncancerous regions. These data indicate that the level of three telomerase-associated genes (i.e., hTR, TEP1 mRNA, hTERT mRNA), do not reflect telomerase activity, and the RNA levels of these genes are not useful markers for diagnosing gastric cancer.
Subject(s)
Gene Expression , Stomach Neoplasms/enzymology , Telomerase/metabolism , Carrier Proteins/metabolism , DNA-Binding Proteins , Humans , RNA/metabolism , RNA-Binding Proteins , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
We established a new cell line, HPC-3P4a, with high peritoneal disseminated potential in nude mice. HPC-3P4a was derived from a human pancreatic carcinoma cell line (HPC-3) that had low capacity for peritoneal dissemination. HPC-3P4a developed peritoneal dissemination in 10 of 11 (90.9%) cases, whereas parental HPC-3 developed peritoneal dissemination in one of six (16.7%) cases. The metastatic foci in the peritoneum showed essentially the same histologic appearance of parental involvement. The tumorigenicity, motility, and adhesive activity of HPC-3P4a to the extracellular matrix were stronger than were those of the HPC-3. In FACS analysis, HPC-3P4a significantly increased the expression of alpha6 and alpha(v)beta5 integrins, while it decreased alpha2 integrin, hCD44H, and hCD44v 10, as compared with HPC-3. The VEGF production of HPC-3P4a was significantly lower than that of HPC-3. Analysis of gene macroarrays showed a variety of cytokines, interleukin, and other immunomodulatory, and their receptors were up-regulated and down-regulated on an mRNA level in HPC-3P4a cells, compared with HPC-3 cells. Intrasplenic injection of HPC-3P4a produced no liver metastasis. We named our original highly liver metastatic cell line HPC-3H4 (previously reported). This HPC-3H4 cell was established by repeated intrasplenic injection from parental cell HPC-3; thus, it developed high liver metastasis. Moreover, HPC-3H4 developed peritoneal dissemination by intra-abdominal injection. In contrast, HPC-3P4a did not develop liver metastasis by intrasplenic injection. These findings are very interesting and might suggest that the process of hematogenous metastasis differed from that of peritoneal dissemination. Thus, this cell line may be useful for investigating the mechanism of peritoneal dissemination in human pancreatic cancer.
Subject(s)
Adenocarcinoma/secondary , Pancreatic Neoplasms/pathology , Peritoneal Neoplasms/secondary , Adenocarcinoma/pathology , Animals , Antigens, CD/genetics , Cell Adhesion , Cell Adhesion Molecules/analysis , Cytokines/biosynthesis , Cytokines/genetics , DNA/analysis , Endothelial Growth Factors/genetics , Female , Flow Cytometry , Gene Expression , Humans , Integrin alpha6 , Interleukins/genetics , Lymphokines/genetics , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplasm Transplantation , Peritoneal Neoplasms/pathology , Ploidies , RNA, Messenger/analysis , Tumor Cells, Cultured , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth FactorsABSTRACT
BACKGROUND: In this study we investigated expression profiles of the anti-apoptotic gene survivin in malignant human haematopoietic cells. MATERIALS AND METHODS: Using a quantitative TaqMan reverse transcription-polymerase chain reaction, survivin and bcl-2 mRNA expression were examined in 12 malignant haematopoietic cell lines, in 21 patients with haematopoietic malignancies and in normal leukocyte fractions. RESULTS: Survivin mRNA levels, demonstrable in all 12 malignant cell lines, differed but showed no relationship to the cell of origin. Conversely, no survivin mRNA expression was detected in normal leukocyte fractions. Further, survivin mRNA was expressed in 16 out of 21 patients with malignancies. Five days after treatment of HL-60 cells with a combination of all-trans retinoic acid and tumor necrosis factor, survivin expression decreased to 14.1% of that in untreated cells. Further, survivin mRNA expression in K-562/ADR cells with acquired resistance to adriamycin was 1.7 times that in parent K-562 cells. CONCLUSION: Our results indicated the possibility that quantitation of survivin mRNA expression is a useful tool for the detection of haematopoietic tumor cells in clinical laboratory test and that survivin could be a target for treatment of haematopoietic malignancies.
Subject(s)
Apoptosis/genetics , Hematopoietic Stem Cells/metabolism , Leukemia/metabolism , Lymphoma, Non-Hodgkin/metabolism , Microtubule-Associated Proteins , Neoplasm Proteins/genetics , Neoplastic Stem Cells/metabolism , Proteins/genetics , RNA, Messenger/biosynthesis , RNA, Neoplasm/biosynthesis , Adolescent , Adult , Aged , Cell Differentiation/drug effects , Child , Child, Preschool , Doxorubicin/pharmacology , Drug Resistance, Neoplasm , Female , Gene Expression Regulation, Neoplastic , HL-60 Cells/drug effects , HL-60 Cells/metabolism , Hematopoietic Stem Cells/pathology , Humans , Infant, Newborn , Inhibitor of Apoptosis Proteins , K562 Cells/drug effects , K562 Cells/metabolism , Leukemia/genetics , Leukemia/pathology , Lymphoma, Non-Hodgkin/genetics , Lymphoma, Non-Hodgkin/pathology , Male , Middle Aged , Neoplastic Stem Cells/pathology , RNA, Messenger/genetics , RNA, Neoplasm/genetics , Reverse Transcriptase Polymerase Chain Reaction , Survivin , Tretinoin/pharmacology , Tumor Cells, Cultured/drug effects , Tumor Necrosis Factor-alpha/pharmacologySubject(s)
Gastric Mucosa/chemistry , Neoplasm Proteins/genetics , RNA, Messenger/analysis , Trans-Activators/genetics , Gastric Mucosa/metabolism , Gastritis/metabolism , Humans , Metaplasia/metabolism , Neoplasm Proteins/biosynthesis , Securin , Stomach Neoplasms/chemistry , Trans-Activators/biosynthesisABSTRACT
BACKGROUND: We studied whether the hematopoietic progenitor cell (HPC) count in peripheral blood as evaluated by an automated counter, the Sysmex SE-9000, correlated with CD34 positive (+) cell count and therefore could guide the timing of peripheral blood stem cell (PBSC) harvest. MATERIALS AND METHODS: HPC count and flow cytometric CD34+ cell count were measured in 90 peripheral blood samples and 30PBSC samples. The correlation between HPC count and apheretic CD34+ cell yield was examined in 19 patients. RESULTS: HPC count showed good correlations with CD34+ cell count in peripheral blood (r = 0.699) and PBSC (r = 0.892). The correlation between peripheral blood HPC count and apheretic CD34+ cell yield also was good (r = 0.789). CONCLUSION: Automated HPC counting can be used as a screening test to guide the timing of PBSC harvest.