Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters











Database
Language
Publication year range
1.
Metab Eng Commun ; 16: e00221, 2023 Jun.
Article in English | MEDLINE | ID: mdl-37006831

ABSTRACT

Isoprene is a clear, colorless, volatile 5-carbon hydrocarbon that is one monomer of all cellular isoprenoids and a platform chemical with multiple applications in industry. Many plants have evolved isoprene synthases (IspSs) with the capacity to liberate isoprene from dimethylallyl diphosphate (DMADP) as part of cellular thermotolerance mechanisms. Isoprene is hydrophobic and volatile, rapidly leaves plant tissues and is one of the main carbon emission sources from vegetation globally. The universality of isoprenoid metabolism allows volatile isoprene production from microbes expressing heterologous IspSs. Here, we compared heterologous overexpression from the nuclear genome and localization into the plastid of four plant terpene synthases (TPs) in the green microalga Chlamydomonas reinhardtii. Using sealed vial mixotrophic cultivation, direct quantification of isoprene production was achieved from the headspace of living cultures, with the highest isoprene production observed in algae expressing the Ipomoea batatas IspS. Perturbations of the downstream carotenoid pathway through keto carotenoid biosynthesis enhanced isoprene titers, which could be further enhanced by increasing flux towards DMADP through heterologous co-expression of a yeast isopentenyl-DP delta isomerase. Multiplexed controlled-environment testing revealed that cultivation temperature, rather than illumination intensity, was the main factor affecting isoprene yield from the engineered alga. This is the first report of heterologous isoprene production from a eukaryotic alga and sets a foundation for further exploration of carbon conversion to this commodity chemical.

2.
Front Microbiol ; 10: 1112, 2019.
Article in English | MEDLINE | ID: mdl-31214129

ABSTRACT

Aeolian dust exerts a considerable influence on atmospheric and oceanic conditions negatively impacting human health, particularly in arid and semi-arid regions like Saudi Arabia. Aeolian dust is often characterized by its mineral and chemical composition; however, there is a microbiological component of natural aerosols that has received comparatively little attention. Moreover, the amount of materials suspended in the atmosphere is highly variable from day to day. Thus, understanding the variability of atmospheric dust loads and suspended microbes throughout the year is essential to clarify the possible effects of dust on the Red Sea ecosystem. Here, we present the first estimates of dust and microbial loads at a coastal site on the Red Sea over a 2-year period, supplemented with measurements from dust samples collected along the Red Sea basin in offshore waters. Weekly average dust loads from a coastal site on the Red Sea ranged from 4.6 to 646.11 µg m-3, while the abundance of airborne prokaryotic cells and viral-like particles (VLPs) ranged from 77,967 to 1,203,792 cells m-3 and from 69,615 to 3,104,758 particles m-3, respectively. To the best of our knowledge, these are the first estimates of airborne microbial abundance in this region. The elevated concentrations of resuspended dust particles and suspended microbes found in the air indicate that airborne microbes may potentially have a large impact on human health and on the Red Sea ecosystem.

SELECTION OF CITATIONS
SEARCH DETAIL