Effect of textile colour on vector mosquito host selection: a simulated field study in Mali, West Africa.

BACKGROUND: The effect of clothing colour on the biting rates of different vector mosquito species is not well understood. Studies under tropical field conditions are lacking. This study aimed to determine the influence of clothing colours on mosquito biting rates in rural and suburban settings in West Africa. METHODS: We performed a simulated field study in a suburban and a rural site in Mali using Mosquito-Magnet traps utilizing CO2 and other attractants, which were covered with black, white, and black/white striped textile sheets covers. These targets operated continuously for 10 consecutive days with bright nights (around full moon) and 10 consecutive days with dark nights (around new moon). Trapped mosquitoes were collected and catch rates counted hourly. Mosquitoes were morphologically identified to the species complex level (Anopheles gambiae s.l. and Culex pipiens s.l.) or species level (Aedes aegypti). A subset of Anopheles specimens were further identified by molecular methods. RESULTS: Under bright-night conditions, An. gambiae s.l. was significantly more attracted to black targets than to white and striped targets; during dark nights, no target preference was noted. During bright nights, Cx. pipiens s.l. was significantly more attracted to black and striped targets than to white targets; a similar trend was noted during dark nights (not significant). For day-active Ae. aegypti, striped targets were more attractive than the other targets and black were more attractive than white targets. CONCLUSIONS: The study firstly demonstrated that under field conditions in Mali, West Africa, mosquito catch rates were influenced by different clothing colours, depending on mosquito species and light conditions. Overall, light colours were least attractive to host-seeking mosquitoes. Using white or other light-coloured clothing can potentially reduce bite exposure and risk of disease transmission in endemic tropical regions.

Description of two new species of Dinometa from East Africa with remarks on D. maputuana (Lepidoptera, Lasiocampidae, Lasiocampinae).

The genus Dinometa Aurivillius, 1927 (type species Gastroplakaeis maputuana Wichgraf, 1906) is reviewed, with two new species described: Dinometa ethani sp. n. from Tanzania and Dinometa abigailae sp. n. from Kenya and Tanzania. All Dinometa species showed no significant differences in male genitalia, but D. ethani sp. n. and D. abigailae sp. n. are allopatric with D. maputuana. D. abigailae sp. n. has specific reddish spots on hindwings that distinguishes it from closely distributed D. ethani sp. n. Two D. maputuana specimens collected at the same night and locality in the Republic of South Africa have an intraspecific variation of 1.52% in cytochrome c oxidase subunit I, while all barcoded D. maputuana are 2.432.74% distant from D. ethani sp. n. Adults, male genitalia and distribution maps of all three species are illustrated.

Phylogeography of Two Enigmatic Sulphur Butterflies, Colias mongola Alphéraky, 1897 and Colias tamerlana Staudinger, 1897 (Lepidoptera, Pieridae), with Relations to Wolbachia Infection.

The genus Colias Fabricius, 1807 includes numerous taxa and forms with uncertain status and taxonomic position. Among such taxa are Colias mongola Alphéraky, 1897 and Colias tamerlana Staudinger, 1897, interpreted in the literature either as conspecific forms, as subspecies of different but morphologically somewhat similar Colias species or as distinct species-level taxa. Based on mitochondrial and nuclear DNA markers, we reconstructed a phylogeographic pattern of the taxa in question. We recover and include in our analysis DNA barcodes of the century-old type specimens, the lectotype of C. tamerlana deposited in the Natural History Museum (Museum für Naturkunde), Berlin, Germany (ZMHU) and the paralectotype of C. tamerlana and the lectotype of C. mongola deposited in the Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia (ZISP). Our analysis grouped all specimens within four (HP_I-HP_IV) deeply divergent but geographically poorly structured clades which did not support nonconspecifity of C. mongola-C. tamerlana. We also show that all studied females of the widely distributed haplogroup HP_II were infected with a single Wolbachia strain belonging to the supergroup B, while the males of this haplogroup, as well as all other investigated specimens of both sexes, were not infected. Our data highlight the relevance of large-scale sampling dataset analysis and the need for testing for Wolbachia infection to avoid erroneous phylogenetic reconstructions and species misidentification.

Notes on Central Asian Furcula with description of a new species.

Genitalia of the female holotype of the Central Asian species Furcula terminata (Wiltshire, 1958) (TL: northeastern Afghanistan) were studied for the first time. They showed a clear similarity with the genitalia of Furcula gorbunovi Schintlmeister, 1998 (TL: Gissar Range, Tajikistan) and Furcula mimonovi Schintlmeister, 1998 (TL: Karakalpakstan, Uzbekistan). Males of F. terminata from Afghanistan were not found in the collections. However, the specimens from neighboring Tajikistan and topotypes of F. gorbunovi and F. mimonovi were studied and all of them have pointed apex of the costal process of valva specific for the bifida-group, not the furcula-group as suggested previously. Considering the morphological similarity and overlapping distribution areas of the three studied taxa, two new synonyms are established for F. terminata: F. gorbunovi syn. n. and Furcula mimonovi syn. n. The population of Tian Shan and Alay Range in Kyrgyzstan and Uzbekistan, previously regarded as F. terminata, are described here as a new species based on differences in the genitalia of both sexes.

New records of Lasiocampidae (Lepidoptera) from Zanzibar Island with taxonomic notes and description of one new species of Odontopacha Aurivillius, 1909.

Seven genera and seven species of Lasiocampidae are newly recorded from the Zanzibar Island (Unguja): Bombycopsis C. & R. Felder, 1874 with Bombycopsis nigrovittata Aurivillius, 1927; Pallastica Zolotuhin & Gurkovich, 2009 with an unidentified species; Dollmania Tams, 1930 with an unidentified species; Mallocampa Aurivillius, 1902 with Mallocampa leighi Aurivillius, 1922; Eucraera Tams, 1930 with Eucraera witti Prozorov, 2016; Philotherma Möschler, 1887 with Philotherma montibia Strand, 1912; and Odontopacha Aurivillius, 1909 with Odontopacha fenestrata Aurivillius, 1909. The species are followed with taxonomic notes updating the status and distribution of the taxa. Bombycopsis nigrovittata is shown to have the maximum p-distance of 0.3% in cytochrome c oxidase I from Bombycopsis pallida Joannou & Krüger, 2009. Two specimens of Pallastica sp. from Zanzibar are different in wing coloration but identical genetically, both are 0.8-1.2% far from sequenced specimens collected in southern Malawi and eastern Zimbabwe and altogether 3.0-3.8% far from the Zambian and Malawian populations considered to be Pallastica pallens (Bethune-Baker, 1908). The barcoding revealed two distinct lineages of Dollmania in Tanzania with a p-distance of 3.5-3.7% between them, neither can be attributed to either Dollmania marwitzi (Strand, 1913) or Dollmania reussi (Strand, 1913) until the primary types or fresh topotypes are sequenced. The species Ph. montibia is taken out from the synonymy to Philotherma rosa (Druce, 1887) and is stated to be a bona species because of the difference in wing pattern and p-distance of 5.7-5.9%. A new species of the genus Odontopacha - Odontopacha dargei sp. n. - is described from southern Kenya and northern Tanzania where it occurs sympatrically with O. fenestrata. It differs from O. fenestrata by the paler coloration with the spotted external fascia on both wings and a p-distance of 3.04-3.65%. Lectotypes for D. marwitzi and Ph. montibia are established. Mallocampa leighi is recorded from Tanzania for the first time. Females of Chrysopsyche lutulenta Tams, 1923 earlier recorded from Zanzibar Island are figured and the species is recorded from DRC for the first time.

Phragmacossia bozanoia new species from southern Greece with some notes on Phragmataecia and Phragmacossia (Lepidoptera, Cossidae, Zeuzerinae).

The taxonomic relationship between the genera Phragmataecia Newman, 1850 and Phragmacossia Schawerda, 1924 is considered. Diagnostic characters and distribution of the species Phragmataecia castaneae (Hbner, 1790), Phragmataecia albida (Erschoff, 1874), Phragmacossia ariana (Grum-Grshimailo, 1899), Phragmacossia territa (Staudinger, 1879), and Phragmacossia minos Reisser, 1963 are studied in detail. A new species, Phragmacossia bozanoi sp. n., is described from the Peloponnese Peninsula and the South Greek mainland.

A new genus of Afrotropical Lasiocampini: Revaya gen. n. (Lepidoptera, Lasiocampidae, Lasiocampinae).

A new genus Revaya gen. n. with Revaya yahya sp. n. (type-species) and Revaya edita sp. n. are described. A detailed diagnosis for the new genus is provided and compared with three closely related genera: Catalebeda Aurivillius, 1902; Ptyssophlebia Berio, 1937; and Oplometa, Aurivillius 1894. Male genitalia of Oplometa cassandra (Druce, 1887) and female genitalia of Ptyssophlebia discocellularis (Strand, 1912) are illustrated for the first time. Catalebeda producta, P. discocellularis and O. cassandra are recorded for the first time in the Democratic Republic of the Congo.

Revision of the Lemonia taraxaci complex, with a description of a new species from Italy and clarification of the status of Lemonia strigata (Lepidoptera: Brahmaeidae: Lemoniinae).

The species Lemonia taraxaci (Denis & Schiffermüller, 1755) is divided into three species based on distribution areas and cytochrome c oxidase subunit 1 (COI) distances above 2%: Central European Lemonia taraxaci (type locality: Austria, Vienna area), Eastern European to South Siberian Lemonia sibirica Wnukowsky, 1934 stat. rev., bona sp. (type locality: Russia, Novosibirsk Oblast, Ozero-Karachi and Russia, Altai Krai, Kornilovo), and Italian Lemonia italiana sp. n. (type locality: Italy, Calabria centr., Sila grd., Casali del Manco, San Nicola Silano (CS)). Authorship of Lemonia strigata Antoshin & Zolotuhin, 2011 is reconsidered. The close relation between the typical L. strigata and taraxaci-like adults from the Balkans is proven genetically. According to this, L. strigata is suggested to be a junior synonym of taraxaci-looking Lemonia taraxaci var. montana Buresch, 1915 (type locality: Bulgaria, Rhodope Mts, Musala), although further DNA investigation is needed to prove this hypothesis.

Identification of Natural Hybrids between Ahlbergia frivaldszkyi (Lederer, 1853) and Callophrys rubi (Linnaeus, 1758) (Lepidoptera, Lycaenidae) Using Mitochondrial and Nuclear Markers.

Natural hybridization is rather widespread and common in animals and can have important evolutionary consequences. In terms of taxonomy, exploring hybridization and introgression is crucial in defining species boundaries and testing taxonomic hypotheses. In the present paper, we report on natural hybrid specimens between Ahlbergia frivaldszkyi (Lederer, 1853) and Callophrys rubi (Linnaeus, 1758). To test the hypothesis of their hybrid origin, we employed the molecular mitochondrial (COI gene) and nuclear (wingless, RPS5, and Ca-ATPase genes) markers commonly used in phylogenetic studies and explored the morphology of the specimens. Our analysis revealed that hybrids bear mitochondrial haplotypes of C. rubi, while nuclear fragments are heterozygous, sharing a combination of A. frivaldszkyi and C. rubi lineages. The hybrid specimens combine morphological characters of both genera. Our results for the first time empirically demonstrate the possibility of genetic introgression between these species and between the genera Callophrys and Ahlbergia on the whole.

A new species of Typhonoya Prozorov (Lepidoptera, Lasiocampidae, Lasiocampinae, Gastropachini) from the moist broadleaf forest of the Democratic Republic of the Congo.

The second species of the genus Typhonoya Prozorov 2011T. kravchenkoi Prozorov, Mller Zolotuhin sp. n. from the Democratic Republic of the Congo is described. A previously monotypic genus becomes bitypic. One paratype specimen was collected in the buffer zone of the southern part of the Salonga National Park during a one year-long stationary expedition (June 2017June 2018). Spread imagoes, heads, legs, venations and genitalia of both species are figured and compared. Additional notes on the genus description and previously known species are added.

A world catalogue of Xyleutes Hbner, 1820 (Lepidoptera: Cossidae: Zeuzerinae) with description of a new species from the Western Ghats of India.

A new species of carpenter moth Xyleutes ramamurthyi Yakovlev Sankararaman, sp. nov. (Lepidoptera, Cossidae, Zeuzerinae) is described as new to science from the Western Ghats of south India. The identification key and species catalog of the genus is provided. In this study, we illustrated with images of all the species of the genus and morphological structures of the new species. The photos of biotopes and the map of new species distribution are provided.

Redescription and review of the Neotropical genus Carohamilia Dyar, 1940 (Lepidoptera: Cossidae: Zeuzerinae).

Based on the study of type specimens, we provide a redescription of the little known tropical genus of Cossidae (Lepidoptera), Carohamilia Dyar, 1940 (type species - Carohamilia ophelia (Schaus, 1921)). We establish the new combinations: Carohamilia masoni (Schaus, 1894), comb. n., Carohamilia terrafirma (Schaus, 1911), comb. n., Carohamilia poam (Dyar, 1918), comb. n., Schreiteriana lineaeplena (Dognin, 1911), comb. n., and a new synonym: Schreiteriana lineaeplena (Dognin, 1911) = Schreiteriana urcuchillay Yakovlev, Penco and Witt, 2017, syn. n.

A new species of Carpenter Moths (Lepidoptera, Cossidae) from Tarbagatai (NE Kazakhstan) and Altai (SW Siberia, Russia) Mountains.

We described a new cossid species, Dyspessa ulgen sp. nov. from the Tarbagatai and Altai Mountains and compared it to other taxa of Dyspessa reported from the region (D. tristis, D. saldaitisi, D. saissanica), as well as to morphologically similar D. ulula. The new species is most closely related to D. ulula but differs from the latter in the characteristics of the male genitalia, wing pattern, and molecular data (a 658 bp fragment of the mitochondrial COI gene).

Two new taxa of Plebejus Kluk, 1780 (Lepidoptera, Lycaenidae) from the Gobi desert, Mongolia.

Plebejus chrisreai sp. n. and P. anikini azhbogdo ssp. n. are described from the Dzhungarian Gobi desert and the Transaltajan Gobi desert respectively. External distinctions as well as distinctions of the male genitalia are given for the new taxa and two related species known from Mongolia: Plebejus anikini Yakovlev, 2012 and P. germani Yakovlev, 2012. Genitalia of all the taxa mentioned are illustrated, the aedeagus and valva bear the most valuable taxonomic characters. Females of the new species have an unusual whitish colour of the upperside not known for the christophi species complex before. The distribution of Mongolian taxa and relatives from East Kazakhstan is discussed.

Two new species of the genus Givarbela Clench, 1957 (Lepidoptera: Cossidae: Hypoptinae) from South Neotropics.

Givarbela steinbachi was described as a new genus and species by Clench (1957), based on 21 specimens from central Bolivia (Prov. del Sara; Buena Vista and Rio Japacani). He indicated that Givarbela (Figs 1‒8) belongs to the Langsdorfia-Givira group of genera but differs from them by the following combination of characters: "R2 stalked with R3-R4, R5 free; the long palpi; absence of fore tibial epiphysis; absence of median cell-vein on fore wing and the open cell-end there; stalked M2-M3 on hind wing; absence of all but traces of a single hind wing anal vein; short hind wing cell; deeply excised hind wing costa" (Clench 1957).

Preliminary list of Cossidae sensu str. (Lepidoptera, Cossoidea: Cossidae: Cossinae amp; Zeuzerinae) of the Republic of Angola with description of a new Strigocossus species.

An annotated list of the Cossidae of the Republic of Angola, including 24 species from 12 genera in two subfamilies, is presented. Thirteen species of Cossidae are reported for the first time from Angola. Strigocossus otti is described as a new species, sp. nov.

Global phylogeography reveals the origin and the evolutionary history of the gypsy moth (Lepidoptera, Erebidae).

We examined the global phylogeography of gypsy moth (Lymantria dispar L.) using molecular data based on mitochondrial and nuclear genes. Populations from all biogeographic regions of the native and introduced range of L. dispar, were sampled to fully document intraspecific and subspecies variation, identify potential cryptic species, and to clarify the relationships among major phylogeographic lineages. We recovered three major mtDNA lineages of L. dispar: Transcaucasia; East Asia + Japan; and Europe + Central Asia. The circumscription of these lineages is only partially consistent with the current taxonomic concept (i.e., L. dispar dispar; L. dispar asiatica; L. dispar japonica), with the following important discrepancies: (1) north-central Asian populations, including topotypical populations of L. dispar asiatica, may be more closely related to European rather than Asian segregates, which would require the synonymization of the taxon asiatica and establishment of a new name; (2) the Japanese populations (L. d. japonica) are not distinct from east Asian populations; (3) the presence of a distinct, unnamed mitogenomic lineage endemic to the Trancaucasus region. We demonstrated that the population from Transcaucasia contains the highest mitochondrial haplotype diversity among L. dispar, potentially indicative of an ancestral area for the entire dispar-group. Our study corroborates the endemic Hokkaido, Japan taxon Lymantria umbrosa (Butler) as the sister group to all other L. dispar populations, but the applicability of the names umbrosa versus hokkaidoensis Goldschmidt needs to be re-evaluated. The ancestral area analysis suggest that Japan was likely colonized via Sakhalin ∼1 Mya, in contrast to previous studies which have suggested colonization of the Japanese archipelago via the Korean Peninsula. Lastly, mitogenomic variation within L. dispar is incongruent with phylogenies based on nuclear DNA, as nDNA gene phylogenies did not recover the three major mtDNA lineages, and also failed to recover L. dispar and L. umbrosa as reciprocally monophyletic.

A DNA-based description of a new carpenter moth species (Lepidoptera: Cossidae) from Morocco.

We used a combination of morphological data (genitalia structure) and a molecular marker (a 658bp fragment of the COI gene) to demonstrate that carpenter moth populations from central and southern Morocco, previously identified as Cossus cossus (Linnaeus, 1758) based on external morphology, represent a new species, described herein as C. romantsovi Yakovlev Shapoval, sp. n. The genetic divergence of the new species with respect to other members of genus Cossus is significant and includes at least 23 fixed nucleotide substitutions in the 658 bp of the COI barcode.

Description of male of the rarest European Carpenter-moth Stygioides persephone (Reisser, 1962) (Lepidoptera: Cossidae).

Despite the generally good knowledge of the European fauna of Cossidae, several new species (Lepidoptera) have been described in recent years: Dyspessa kostyuki Yakovlev, 2005 (type locality Ukraine, [Lugansk region], "Proval'skaya Stepp" Naturschutsgebiete), D. aphrodite Yakovlev & Witt, 2007 (type locality Greece, Peloponnes, Mega Spileon), and Stygia nilssoni Saldaitis & Yakovlev, 2008 (type locality Islas Canarias, Gran Canaria, Puerto de Mogan) (Yakovlev 2005; Yakovlev & Witt 2007; Saldaitis & Yakovlev 2008). However, a number of European cossids are still poorly known.

Three new species and one new subspecies of Deserticossus Yakovlev, 2006 (Lepidoptera: Cossidae) from Kazakhstan, Kyrgyzstan and Russia, with world catalogue of the genus.

Three new species and one new subspecies of the genus Deserticossus Yakovlev, 2006 are described: Deserticossus doroshkini Yakovlev & Witt sp. nov. from eastern Kazakhstan (Tarbagatai Mts.), D. selevini Yakovlev & Witt sp. nov. from southeastern Kazakhstan (Malye Boguty Mts.), D. kamelini Yakovlev & Witt sp. nov. from Kyrgyzstan (Fergana Valley), and D. tsingtauana didenkoi Yakovlev & Witt subsp. nov. from Russia (Southern Siberia, Buryatia Republic). The described species and subspecies of Deserticossus are listed, with notes on the type material, synonymies, and distribution for each taxon.