Prevalence and antibacterial susceptibilities of Arcobacter spp. and Campylobacter spp. from fresh vegetables.

This study was aimed at the isolation and identification of Arcobacter spp. and Campylobacter spp. from fresh vegetables sold at district markets in the Kayseri province, and at the determination of the antibacterial susceptibility of the recovered isolates. For this purpose, a total of 175 vegetable samples, including 35 spinach, 35 lettuce, 35 parsley, 35 arugula, and 35 radish samples, were collected. While the pre-enrichment and membrane filtration techniques were used for the isolation of Arcobacter spp., the pre-enrichment and direct inoculation methods were used for the isolation of Campylobacter spp. The isolates were identified by means of phenotypic tests and the polymerase chain reaction (PCR), using genus- and species-specific primers. In addition, the susceptibilities of the isolates to amoxicillin-clavulanic acid, enrofloxacin, erythromycin, gentamicin, neomycin, streptomycin, and tetracycline were determined by the disk diffusion method. Out of the 175 vegetable samples tested, 93 (53.14%) were found to be positive for Arcobacter spp., and 119 Arcobacter spp. isolates were recovered from these 93 positive samples. All of the samples examined were found to be negative for Campylobacter spp. One hundred one (86%) and 14 (10%) of the 119 Arcobacter isolates obtained were identified as A. butzleri and A. cryaerophilus, respectively, but four isolates could not be identified at the species level by mPCR. Mixed contamination with more than one species and/or genotypes of Arcobacter was detected in 24 of the positive samples. While all of the Arcobacter isolates were susceptible to erythromycin, gentamicin, streptomycin, and tetracycline, 2 (1.68%), 2 (1.68%), and 5 (4.20%) isolates were resistant to amoxicillin/clavulanic acid, enrofloxacin, and neomycin, respectively. Consequently, the determination of a high prevalence of arcobacters and mixed contamination with more than one species and/or genotypes of arcobacters in vegetables often consumed raw by humans demonstrated that the consumption of raw vegetables may be a risk to the public health.

The effects of stocking density on slaughter performance and some meat quality parameters of Pekin ducks.

The effects of stocking density on slaughter performance and meat quality were primarily investigated in this research. A total of 240 Pekin ducks were used, and they were reared until slaughter age (42 d) in three different stocking density groups (three, five and seven ducklings m - 2 ). To compare the slaughter performance of the ducklings' live weight, carcass weight, carcass yield, thigh and breast meat weight and yield, and edible giblet weight (heart, liver and gizzard) were investigated. The meat quality was compared between the treatment groups based on dry matter ratio, cooking loss, water-holding capacity, pH values, and colour parameters ( L , a , b , c , h and Δ E values). Carcass weight, carcass yield, thigh and breast meat weight were found to decrease in parallel to the increasing stocking density, resulting in a reduction in thigh and breast meat weights and ratios ( P < 0.05 ). Increasing the stocking density decreased the heart weight and positively improved the liver and gizzard ratio ( P < 0.05 ). However, it did not affect the meat quality parameters investigated in this research ( P < 0.05 ). The breast meat of the ducks reared under higher stocking density had higher L , h and Δ E values, lower a value ( P < 0.05 ), and similar b and c values ( P < 0.05 ). Evaluating the overall research findings, it was concluded that increased stocking density when rearing ducks negatively affects the slaughter performance, affecting only breast meat colour and weight of thigh meat with skin in investigated meat quality parameters.