Anisotropy in the Liquefaction Resistance of Fibre Reinforced Sand.

Adding discrete fibres to sand has been seen as a feasible technique to improve sand's strength as well as liquefaction resistance. Considering the anisotropic distribution of fibre orientations, the anisotropy in the liquefaction resistance of the reinforced sand is also introduced using fibres. Here, the triaxial compression and extension test results of unreinforced and fibre-reinforced sand in different density states are provided, from which the anisotropy in the liquefaction resistance of fibre-reinforced sand is demonstrated. Fibre reinforcement improves the liquefaction resistance of sand by introducing both the densifying effect and the confining effect. The inclusion of fibres increases both the slope and the intercept of the strength envelope in comparison with the unreinforced sand under triaxial compression, while the strength envelope is not affected by fibres under triaxial extension. Stress contribution of fibres makes the ESP of the composite under undrained loading reverse its direction to develop even though the phase transformation is absent. The stress ratio initiating the ESP reversal is irrespective of the fibre content but dependent on the density state under triaxial compression. Under triaxial extension, the stress ratio initiating the ESP reversal remains the same in the samples with varied density states and fibre contents. The mechanism correlating to the strength envelope and ESP reversal of the fibre-reinforced sand was demonstrated following a rule of mixture based constitutive modelling framework. By introducing an alternatively defined pore pressure ratio that incorporates the stress contribution of fibres, the liquefaction state of the fibre reinforced sand is reasonably assessed. Liquefaction remains absent in the sand once the fibres are mixed. The anisotropy in the liquefaction resistance of fibre-reinforced sand arises, as the predominant role played by the fibres to suppress the liquefaction is different when varied loading paths are involved, which is sourced from the anisotropic distribution of fibre orientations.

Alkylamine-Grafted Molybdenum Disulfide Nanosheets for Enhanced Dispersion Stability and Lubricity Properties.

The unique structure and ultralow interlayer shear strength give molybdenum disulfide (MoS2) materials a broad prospect for energy savings, economic benefits, and extended operating life of lubrication systems. Herein, we prepared an effective integration strategy to prepare novel small-sized and chemically grafted MoS2 to solve the problems of poor dispersibility and easy agglomeration of MoS2. The MoS2 powder was stripped and oxidized to generate active centers using acid oxidation and high-speed ultrasonic crushing to obtain two different types of alkylamine chemically, covalently grafted, oxidized MoS2 nanosheets as lubricant additives to achieve friction reduction and antiwear. The chemical changes and structural characteristics of different types of alkylamine molecules upon covalent interaction with oxidized MoS2 were investigated in detail by FTIR, XPS, TGA, XRD, and TEM analyses. The results showed that the alkylamine-grafted MoS2 oxide nanosheets had good dispersion in 15# industrial white oil, and friction experiments confirmed that the alkylamine-grafted MoS2 oxide (MoS2-O-OLA) nanosheets exhibited better friction and wear resistance such that, compared with pure 15# industrial white oil, the 0.02 wt % MoS2-O-OLA nanosheets could significantly reduce friction (36.2%) and wear (22.4%). The field-emission scanning electron microscopy (FESEM) and EDS analyses of the wear surface showed that MoS2-O-OLA nanosheets play an important role in improving tribological properties by generating interlayer slippage at the steel ball contact interface, thereby forming surface protection and a uniform oil film.

Automatic multi-tissue segmentation in pancreatic pathological images with selected multi-scale attention network.

The morphology of tissues in pathological images has been used routinely by pathologists to assess the degree of malignancy of pancreatic ductal adenocarcinoma (PDAC). Automatic and accurate segmentation of tumor cells and their surrounding tissues is often a crucial step to obtain reliable morphological statistics. Nonetheless, it is still a challenge due to the great variation of appearance and morphology. In this paper, a selected multi-scale attention network (SMANet) is proposed to segment tumor cells, blood vessels, nerves, islets and ducts in pancreatic pathological images. The selected multi-scale attention module is proposed to enhance effective information, supplement useful information and suppress redundant information at different scales from the encoder and decoder. It includes selection unit (SU) module and multi-scale attention (MA) module. The selection unit module can effectively filter features. The multi-scale attention module enhances effective information through spatial attention and channel attention, and combines different level features to supplement useful information. This helps learn the information of different receptive fields to improve the segmentation of tumor cells, blood vessels and nerves. An original-feature fusion unit is also proposed to supplement the original image information to reduce the under-segmentation of small tissues such as islets and ducts. The proposed method outperforms state-of-the-arts deep learning algorithms on our PDAC pathological images and achieves competitive results on the GlaS challenge dataset. The mDice and mIoU have reached 0.769 and 0.665 in our PDAC dataset.

Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene.

Freshwater shrimp are a rich species group, with a long and problematic taxonomic history attributed to their wide distribution and similar morphological characteristics. Shrimp diversity and species identification are important cornerstones for fisheries management. However, identification based on morphological characteristics is a difficult task for a nonspecialist. Abundant freshwater shrimp species are distributed in the waters of Henan Province, but investigations of freshwater shrimp are limited in this region, especially concerning molecular features. Here, we combined morphology and DNA barcodes to reveal the species diversity of freshwater shrimp in Henan province. A total of 1,200 freshwater shrimp samples were collected from 46 sampling sites, and 222 samples were chosen for further microscopic examination and molecular delimitation. We used tree-based methods (NJ, ML, and bPTP) and distance-based methods (estimation of the paired genetic distances and ABGD) to delimit species. The results showed that there were nine morphospecies based on morphological characteristics; all could effectively be defined by molecular methods, among which bPTP and ABGD defined 13 and 8 MOTUs, respectively. The estimation of the paired genetic distances of K2P and the p-distances had similar results. Mean K2P distances and p-distances within species were both equal to 1.2%. The maximum intraspecific genetic distances of all species were less than 2%, with the exception of Palaemon modestus and M. maculatum. Various analyses have shown that P. modestus and M. maculatum have a large genetic differentiation, which may indicate the existence of cryptic species. By contrast, DNA barcoding could unambiguously discriminate 13 species and detect cryptic diversity. Our results demonstrate the high efficiency of DNA barcoding to delimit freshwater shrimp diversity and detect the presence of cryptic species.

The Chromosome-Level Genome of Triplophysa dalaica (Cypriniformes: Cobitidae) Provides Insights into Its Survival in Extremely Alkaline Environment.

Lake Dali Nur, located in Inner Mongolia, North China, is alkaline, with Triplophysa dalaica one of the three fish species that not only survive, but thrive, in the lake. To investigate the presence of molecular mutations potentially responsible for this adaptation, the whole-genome sequence of the species was sequenced. A total of 126.5 and 106 Gb data, covering nearly 200× of the estimated genome, were generated using long-read sequencing and Hi-C technology, respectively. De novo assembly generated a genome totalled 607.91 Mb, with a contig N50 of 9.27 Mb. Nearly all whole-genome sequences were anchored and oriented onto 25 chromosomes, with telomeres for most chromosomes also being recovered. Repeats comprised approximately 35.01% of the whole genome. A total of 23,925 protein-coding genes were predicted, within which, 98.62% could be functionally annotated. Through comparisons of T. dalaica, T. tibetana, and T. siluroides gene models, a total of 898 genes were identified as likely being subjected to positive selection, with several of them potentially associated with alkaline adaptation, such as sodium bicarbonate cotransporter, SLC4A4. Demographic analyses suggested that the Dali population might have diverged from endemic freshwater Hai River populations, approximately 1 Ma. The high-quality T. dalaica genome, created in this study, not only aids in the analyses of alkaline adaptation, but may also assist in revealing the mysteries of the highly divergent genus Triplophysa in the future.

Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties.

Bacterial type VI secretion systems (T6SSs) inject toxic effectors into adjacent eukaryotic and prokaryotic cells. It is generally thought that this process requires physical contact between the two cells. Here, we provide evidence of contact-independent killing by a T6SS-secreted effector. We show that the pathogen Yersinia pseudotuberculosis uses a T6SS (T6SS-3) to secrete a nuclease effector that kills other bacteria in vitro and facilitates gut colonization in mice. The effector (Tce1) is a small protein that acts as a Ca2+- and Mg2+-dependent DNase, and its toxicity is inhibited by a cognate immunity protein, Tci1. As expected, T6SS-3 mediates canonical, contact-dependent killing by directly injecting Tce1 into adjacent cells. In addition, T6SS-3 also mediates killing of neighboring cells in the absence of cell-to-cell contact, by secreting Tce1 into the extracellular milieu. Efficient contact-independent entry of Tce1 into target cells requires proteins OmpF and BtuB in the outer membrane of target cells. The discovery of a contact-independent, long-range T6SS toxin delivery provides a new perspective for understanding the physiological roles of T6SS in competition. However, the mechanisms mediating contact-independent uptake of Tce1 by target cells remain unclear.

Application of DNA barcoding in fish identification of supermarkets in Henan province, China: More and longer COI gene sequences were obtained by designing new primers.

In recent years, DNA barcode technology has been widely used in food identification, especially in the identification of fish. In China, there are few studies on the authenticity of fish products in Henan province of China. In this study, 179 fish samples were collected from supermarkets in Zhengzhou city and Xinxiang city in Henan province, China. COI gene sequences were obtained with PCR technology by designing specific primers and universal primers. COI gene sequences of all samples were obtained to identify species, which is used to investigate species substitution and mislabeling of the fish sold in the two regional markets. The molecular identification results showed that 28.49% (51/179) fish samples were not consistent with the labels. Substitution of high-price fish by low-price fish was prevalent. For example, halibut (Pleuronectiformes) and cod (Gadus) are replaced by striped catfish (Pangasianodon hypophthalmus), and some merchants label bighead carp (Hypophthalmichthys nobilis) as cod (Gadus), there are also accidental labeling errors (such as labels for greenfin horse-faced filefish (Thamnaconus septentrionalis) have been identified as grass carp (Ctenopharyngodon idella) and Wuchang bream (Megalobrama amblycephala) etc. Most of the samples labeled correctly are the fish of low economic value and the fresh fish. This study shows that almost all the commercial fish can be identified by COI DNA barcoding by newly designed primers. Finally, this study also gives a reference of real species of fish fillet in Henan province in China.