ABSTRACT
Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer-Emmett-Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.
Subject(s)
DNA Probes/chemistry , Gels/chemistry , Nucleotides/analysis , Oligonucleotide Array Sequence Analysis/methods , Organosilicon Compounds/chemistry , Polymers/chemistry , Humans , Magnetic Resonance Spectroscopy , Microscopy, Electron, Scanning , Nitrogen/chemistry , Nucleic Acid Conformation , Nucleotides/genetics , Nucleotides/metabolism , Silicon Dioxide/chemistry , Spectroscopy, Fourier Transform Infrared , TemperatureABSTRACT
A 17-year-old boy presented to the emergency department for severe left flank pain and gross hematuria 1 hour after playing basketball without significant collision. Laboratory tests showed normal renal function and massive hematuria. Abdominal computed tomography scan disclosed a primary megaureter with ruptured kidney. We successfully treated him with ureteral stenting followed by endoscopic ureterotomy and ureteroneocystostomy.