ABSTRACT
Nickel stands out as one of the prevalent heavy metal ionic pollutants found in water. It is urgent to devise a simple, efficient, budget-friendly, highly-selective and proficient method for detecting Ni(II). This work reports an approach to design a nanofluidic diode for the ultrasensitive and label-free detection of nickel ions based on layer-by-layer assembly of polyarginine (PA) and polyglutamic acid (γ-PGA) on the inner surface of asymmetric nanochannels. We can tune the adsorption/desorption characteristics of the asymmetric nanochannels for Ni2+ by adjusting the pH changes, i.e., the PA-γ-PGA modified nanochannels adsorb Ni2+ at pH 6 and desorb at pH 3 in aqueous solution. This pivotal adjustment facilitates the reusable and specific detection of nickel ions with a detection limit of 1 × 10-8 M. Moreover, the system demonstrates commendable stability and recyclability, enhancing its practical applicability. This innovative system holds promise for recognizing and detecting nickel ions in diverse environments such as water, blood, and cells. The robust performance and adaptability of our proposed system instill confidence in its potential for future applications.
ABSTRACT
As a carbon substrate, propionate can be used to synthesize poly(3-hydroxybutyrateco-3-hydroxyvalerate) [PHBV] biopolymer, but high concentrations can inhibit PHBV production. Therefore, novel PHBV producers that can utilize high propionate concentrations are needed. Here, a novel halophile, Halomonas sp. YJ01 was applied to PHBV production via a propionate-dependent pathway, and optimal culture growth conditions were determined. The maximum poly(3-hydroxybutyrate) [PHB] content and yield in the presence of glucose were 89.5 wt% and 5.7 g/L, respectively. This strain utilizes propionate and volatile fatty acids (VFAs) for PHBV accumulation. Multiple genes related to polyhydroxyalkanoate (PHA) synthesis were identified using whole-genome annotation. The PHBV yield and 3HV fraction obtained by strain YJ01 utilizing 15 g/L propionate were 0.86 g/L and 29 mol%, respectively, but in cultures with glucose-propionate, it decreased its copolymer dry weight. This indicates that propionyl-CoA was converted to pyruvate through the 2-methylcitrate cycle (2MCC), which reduced propionate detoxification for the strain.
ABSTRACT
Artificial solid-state nanochannels have garnered considerable attention as promising nanofluidic tools for ion/molecular detection, DNA sequencing, and biomimicry. Recently, nanofluidic devices have emerged as cost-effective detection tools for heavy metal ions by modifying stimuli-responsive materials. In this work, high-purity glycyl-l-histidyl-l-lysine (GHK) peptide is synthesized by using 7-diphenylphosphonooxycoumarin-4-methanol (DPCM) as a protecting group and auxiliary carrier by homogeneous synthesis of photocleavable groups. Subsequently, we developed a GHK-modified asymmetric nanochannel nanofluidic diode by covalently attaching the GHK peptide to the inner surface of the nanochannels. This modification facilitated specific recognition and ultra-trace level detection of Cu2+ ions, achieving a detection limit of 10-15 M. Due to the robust complexing ability between Cu2+ and GHK peptide, the GHK-modified asymmetric nanochannels can form GHK-Cu complexes on the inner surface of nanochannels when Cu2+ passes through the nanochannels. This results in changes of current-potential (I-V) properties, which facilitated Cu2+ detection. Theoretical calculations confirmed the high affinity of the GHK peptide for Cu2+, thereby ensuring excellent Cu2+ selectivity. To evaluate the applicability of our system for detecting Cu2+ in real-world scenarios, we analyzed the concentration of Cu2+ in tap water. The GHK-Cu complexes could be dissociated by adding EDTA to the solution, enabling the regeneration and reuse of this ultrasensitive and label-free Cu2+ detection system using GHK-modified asymmetric multi-nanochannels. We anticipate that the GHK-modified asymmetric nanochannels will find future applications in the label-free detection of Cu2+ in domestic water.
