Resveratrol ameliorates DSS-induced ulcerative colitis by acting on mouse gut microbiota.

OBJECTIVE AND DESIGN: Ulcerative colitis (UC) is a multi-faceted, recurrent immune disorder caused by dextran sulfate sodium (DSS). The intestinal microbiota has multiple functions in the host, so UC requires long-term potent medication. The effect of resveratrol (RSV) has seldom been reported, and this study researched that. Herein, the effect of RSV and Grape seed oil that anti-inflammatory ability in experimental mice was explored, also why RSV altered Gut Microbiota has been researched. MATERIALS AND METHODS: In this experiment, the effects of experimental drugs on colon length in mice with DSS-induced colitis were compared. H&E Staining was performed on serial sections of colon tissues and histological scores were determined for all groups. The expression of cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) in the colon tissue of mice was detected by immunohistochemical staining. In the end, the α-diversity index, sobs index, and rarefaction curve of the cecal and colon microbiota of different groups of mice were measured. Bray-Curtis-based Venn diagram of PCoA (principal coordinate analysis) and OTUs distribution in mouse gut microbiota were obtained. RESULTS: The results showed that the use of 40 mg/kg RSV (high dose) significantly reduced the severity of UC. The use of 10 mg/kg RSV (low dose) significantly reduced the effect of shortened colon length in DSS mice. Compared with the DSS-treated group, the levels of COX-2 and TNF-α in the colon tissues of RSV + DSS-treated mice were significantly decreased. According to this experiment, 19 mouse gut microbiota species had a relative abundance greater than 0.1%, with Beerella, Bacteroides, Helicobacter, Oscillator, and cecum pylori being more abundant in the colon than in the colon. A higher relative abundance of Lachnospira NK4A136 was observed in DSS and RSV groups compared with the control group, whereas the opposite was observed for Alloprevotella. This proves that resveratrol increases the uniformity and diversity of gut microbes to a certain extent, and has a protective effect on the gut.

Geographical distribution and potential distribution prediction of thirteen species of Citrus L. in China.

Using DIVA-GIS software to study the spatial accumulation of Citrus species, an important economic crop in China. Draw the distribution maps of Citrus to concerning altitude and vegetation, and use DIVA-GIS' Bioclim ecological model and maximum information entropy model Maxent software to estimate the potential distribution areas of various Citrus species. The results show that the Citrus genus is located in the south of Qinling Mountains, mainly in the southwest of China and the coast of southeastern China. Sichuan and Chongqing are the most densely distributed regions of Citrus. The distribution of Citrus is closely related to the vegetation type and altitude. The vegetation types in the distribution area is evergreen broad-leaved forest, evergreen shrub, deciduous broad-leaved forest, mixed-leaf forest coverage area, deciduous coniferous forest, farmland, trees, other vegetation coverage, and evergreen coniferous forest. The current potential distribution area of Citrus is mainly in Guizhou, Hubei, Hunan, Guangdong, Guangxi, Yunnan, Sichuan, Chongqing, and other provinces and municipalities and their borders, while the potential distribution area in the future moves northward and enter Henan and southern Gansu. At the same time, climate warming changes the distribution of suitable areas of Citrus, which makes the suitable areas of C. sinensis Osbeck, C. reticulata Blanco, and other crops greatly increased. Planning the planting area will effectively improve the yield and quality in the future. Planning presents new challenges.

Glutamine Regulates Gene Expression Profiles to Increase the Proliferation of Porcine Intestinal Epithelial Cells and the Expansion of Intestinal Stem Cells.

The intestinal epithelium is known for its rapid self-renewal, and glutamine is crucial in providing carbon and nitrogen for biosynthesis. However, understanding how glutamine affects gene expression in the intestinal epithelium is limited, and identifying the essential genes and signals involved in regulating intestinal epithelial cell growth is particularly challenging. In this study, glutamine supplementation exhibited a robust acceleration of intestinal epithelial cell proliferation and stem cell expansion. RNA sequencing indicated diverse transcriptome changes between the control and glutamine supplementation groups, identifying 925 up-regulated and 1152 down-regulated genes. The up-regulated DEGs were enriched in the KEGG pathway of cell cycle and GO terms of DNA replication initiation, regulation of phosphatidylinositol 3-kinase activity, DNA replication, minichromosome maintenance protein (MCM) complex, and ATP binding, whereas the down-regulated DEGs were enriched in the KEGG pathway of p53 signaling pathway, TNF signaling pathway, and JAK-STAT signaling pathway and GO terms of inflammatory response and intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress. Furthermore, GSEA analysis revealed a significant up-regulation of the cell cycle, DNA replication initiation, ATP-dependent RNA helicase activity, and down-regulation of the TNF signaling pathway. The protein-protein association network of the intersecting genes highlighted the significance of DNA replication licensing factors (MCM3, MCM6, and MCM10) in promoting intestinal epithelial growth in response to glutamine. Based on these findings, we propose that glutamine may upregulate DNA replication licensing factors, leading to increased PI3K/Akt signaling and the suppression of TNF, JAK-STAT, and p53 pathways. Consequently, this mechanism results in the proliferation of porcine intestinal epithelial cells and the expansion of intestinal stem cells.

Geographical distribution and predict potential distribution of Cerasus serrulata.

Climate change is closely related to the distribution of plant resources. Cerasus serrulata is an important plant resource in China. The study on the influence of environmental factors on the distribution of suitable areas of C. serrulata is conducive to the protection and development of C. serrulata. In this paper, the distribution information of 238 Chinese Cerasus serrulata plants was processed by DIVA-GIS. The MaxEnt model was used to simulate the current and future distribution, and the ecological distribution and richness of Cerasus were analyzed. The results showed that the Cerasus serrulata was widely distributed in Hebei, Heilongjiang, Shandong, Jiangsu, Zhejiang, Anhui, Jiangxi, Hunan, and Guizhou provinces, mainly in the low and middle elevation areas of 10 to 1200 m. Based on this model, the precipitation of the warmest quarter, the precipitation of the driest month, and the mean temperature of the coldest were the most significant bioclimatic variables affecting the distribution of C. serrulata. In the future, climate change may lead to a slight increase of 2.31% in the area of suitable habitat for Cerasus serrulata, while the optimal habitat will decrease from 20.81 to 14.55%. Therefore, conservation measures should be taken to protect these precious resources.

Effects of climate change on the geographical distribution and potential distribution areas of 35 Millettia Species in China.

Climate change has an extremely important impact on the geographic distribution of plants. The genus Millettia is an important plant resource in China and is widely used in medicine and ornamental industries. Due to the continuous changes of climate and the development and utilization of plant resources of the genus Millettia, it is of great significance to systematically investigate the geographic distribution of plants of the Millettia and their potential distribution under climate change. DIVA-GIS software was used to analyze 3492 plant specimens of 35 species of genus Millettia in the herbarium, and the ecological geographic distribution and richness of Millettia were analyzed, and the MaxEnt model was used to analyze the current and potential distribution in the future. The results show that the genus Millettia is distributed in 30 provinces in China, among which Yunnan and Guangdong provinces are the most distributed. Our model determines that precipitation in the driest month and annual temperature range are the most important bioclimatic variables. Future climate changes will increase the suitable habitat area of M. congestiflora by 16.75%, but other cliff beans Suitable habitats for vines will decrease significantly: M. cinereal by 47.66%, M. oosperma by 39.16%, M. pulchra by 36.04%, M. oraria by - 29.32%, M. nitida by 22.88%, M. dielsiana by 22.72%, M. sericosema by 19.53%, M. championii by 7.77%, M. pachycarpa by 7.72%, M. speciose by 2.05%, M. reticulata by 1.32%. Therefore, targeted measures should be taken to protect and develop these precious plant resources.

Anti-inflammatory Study on the Constituents of Angelica sinensis (Oliv.) Diels, Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav., Angelica pubescence Maxim and Foeniculum vulgare Mill. Essential Oils.

Umbelliferae plants, which are widely used as traditional Chinese medicine because of their characteristics of relieving rheumatism, alleviating fever, circulating blood and easing pain. This experimental study was based on ear edema model caused by 12-O-tetracycline-propylphenol-13-acetic acid (TPA) in mice and compared with the Ibuprofen (Ib) group. Gas chromatography-mass spectrometry (GC-MS) was used to analyse the composition of the essential oils from the four studied Umbelliferae plants (Angelica sinensis (Oliv.) Diels, A. dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav., A. pubescens Maxim and Foeniculum vulgare Mill.). Biologically active components in volatile oils from the four studied Umbelliferae plants were evaluated. The expression levels of inflammatory cytokines Tumor Necrosis Factor-α (TNF-α), Cyclooxygenase-2 (COX-2), Interleukin-6 (IL-6) and RelA (p65) in mouse skin were determined by immunohistochemical method. The refractive index of the four essential oils was calculated. A total of 239 compounds were identified by GC-MS from the four studied plants, and the main constituents were osthole (44.61%, APEOs), obepin (0.59%, APEOs & 86.58%, FVEOs), undecanol (8.58%, ADEOs), α-muurolene (7.95%, ADEOs) and cis-anethol (9.11%, ADEOs). E-ligustilide (0.14%, APEOs & 81.14%, ASEOs), (-)-spathulenol (0.08%, FVEOs & 1.21%, ASEOs), (-)-terpinen-4-ol (4.91%, FVEOs), 2-butylthiolane (5.76%, APEOs) and α-bisabolol (3.80%, APEOs). This study showed that all the essential oils from the four studied Umbelliferae plants contained various lactones, including ligustrongolactone, trans-anisol and imperatorin. According to the results of the TPA induction test in the mouse ear edema model, the essential oils of four Umbelliferae plants reduced the levels of inflammatory cytokines TNF-α, COX-2, IL-6 and p65. All of them showed extraordinary biological activity in anti-inflammatory, so they have potential application value for biomedical products, pharmaceutical preparations, natural functional nutrients and cosmetic additives.

Aromatic-turmerone ameliorates DSS-induced ulcerative colitis via modulating gut microbiota in mice.

Curcuma longa L. is one of the traditional Chinese herbs in the list of medicinal and food homology. Aromatic-turmerone is the main ingredient in turmeric essential oil. The aim of the present study is to investigate the treatment of Aromatic-turmerone on DSS-included colitis and its regulatory effect on intestinal flora disorder. Male KM mice supplemented with different concentration of aromatic-turmerone and mesalazine are subjected to 2% DSS in drinking water to induce colitis. Colon and cecum contents were collected for colitis lesion evaluation and inflammation-related gene analysis and colon contents for gut microbiota. The results show that treatments with Aromatic-turmerone significantly prevents colon shortening, alleviates the damage of colonic tissue, and reduces colonic inflammatory cytokines TNF-α and COX-2. Furthermore, the 16S rDNA gene sequence data indicate that Aromatic-turmerone improve the abundance of bacterial species, maintain some beneficial bacteria, and reduce harmful bacteria. Aromatic-turmerone downregulates the colonic inflammatory cytokines and modulates the abundance of intestinal flora, which is conductive to ameliorates DSS-induced colitis. Regularly intake of the edible herb may be help to prevent ulcerative colitis-related diseases.