Identification of a prognostic signature based on five ferroptosis-related genes for diffuse large B-cell lymphoma.

BACKGROUND: Therapies for diffuse large B-cell lymphoma (DLBCL) are limited due to the diverse gene expression profiles and complicated immune microenvironments, making it an aggressive lymphoma. Beyond this, researches have shown that ferroptosis contributes to tumorigenesis, progression, and metastasis. We thus are interested to dissect the connection between ferroptosis and disease status of DLBCL. We aim at generating a valuable prognosis gene signature for predicting the status of patients of DLBCL, with focus on ferroptosis-related genes (FRGs). OBJECTIVE: To examine the connection between ferroptosis-related genes (FRGs) and clinical outcomes in DLBCL patients based on public datasets. METHODS: An expression profile dataset for DLBCL was downloaded from GSE32918 (https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=gse32918), and a ferroptosis-related gene cluster was obtained from the FerrDb database (http://www. zhounan.org/ferrdb/). A prognostic signature was developed from this gene cluster by applying a least absolute shrinkage and selection operator (LASSO) Cox regression analysis to GSE32918, followed by external validation. Its effectiveness as a biomarker and the prognostic value was determined by a receiver operator characteristic curve mono factor analysis. Finally, functional enrichment was evaluated by the package Cluster Profiler of R. RESULTS: Five ferroptosis-related genes (FRGs) (GOP1, GPX2, SLC7A5, ATF4, and CXCL2) associated with DLBCL were obtained by a multivariate analysis. The prognostic power of these five FRGs was verified by TCGA (https://xenabrowser.net/datapages/?dataset=TCGA.DLBC.sampleMap%2FHiSeqV2_PANCAN&host=https%3A%2F%2Ftcga.xenahubs.net&removeHub=https%3A%2F%2Fxena.treehouse.gi.ucsc.edu%3A44) and GEO (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=gse 32918) datasets, with ROC analyses. KEGG and GO analyses revealed that upregulated genes in the high-risk group based on the gene signature were enriched in receptor interactions and other cancer-related pathways, including pathways related to abnormal metabolism and cell differentiation. CONCLUSION: The newly developed signature involving GOP1, GPX2, SLC7A5, ATF4, and CXCL2 has the potential to serve as a prognostic biomarker. Furthermore, our results provide additional support for the contribution of ferroptosis to DLBCL.

Intrusion Detection System in the Advanced Metering Infrastructure: A Cross-Layer Feature-Fusion CNN-LSTM-Based Approach.

Among the key components of a smart grid, advanced metering infrastructure (AMI) has become the preferred target for network intrusion due to its bidirectional communication and Internet connection. Intrusion detection systems (IDSs) can monitor abnormal information in the AMI network, so they are an important means by which to solve network intrusion. However, the existing methods exhibit a poor ability to detect intrusions in AMI, because they cannot comprehensively consider the temporal and global characteristics of intrusion information. To solve these problems, an AMI intrusion detection model based on the cross-layer feature fusion of a convolutional neural networks (CNN) and long short-term memory (LSTM) networks is proposed in the present work. The model is composed of CNN and LSTM components connected in the form of a cross-layer; the CNN component recognizes regional features to obtain global features, while the LSTM component obtain periodic features by memory function. The two types of features are aggregated to obtain comprehensive features with multi-domain characteristics, which can more accurately identify intrusion information in AMI. Experiments based on the KDD Cup 99 and NSL-KDD datasets demonstrate that the proposed cross-layer feature-fusion CNN-LSTM model is superior to other existing methods.

Single-transverse-mode broadband InAs quantum dot superluminescent light emitting diodes by parity-time symmetry.

Parity-time (PT) symmetry breaking in counterintuitive gain/loss coupled waveguide designs is numerically and theoretically investigated. The PT symmetry mode selection conditions are determined theoretically. Single-transverse-mode broadband InAs quantum dot (QD) superluminescent light emitting diodes (SLEDs) are fabricated and characterized; the PT symmetric broad-area SLEDs contain laterally coupled gain and loss PT- symmetric waveguides. Single-transverse-mode operation is achieved by parity-time symmetry breaking. The broadband SLEDs exhibit a uniform Gaussian-like emission spectrum with the 3-dB bandwidth of 110 nm. Far-field characteristics of the coupled waveguide SLEDs exhibit a single-lobe far-field pattern when the gain and loss waveguides are biased at the injection current of 600 mA and 60 mA, respectively.