ABSTRACT
Seasonal reproduction depends on photoperiod-regulated activation or suppression of the gonadal axis. Recent studies in quail have identified long-day induced TSH-beta expression in the pars tuberalis (PT) as a rapid trigger of gonadal activation. Thyroid-stimulating hormone (TSH) induces type 2 deiodinase (Dio2) in the ependymal cell layer (EC) of the infundibular recess to stimulate the gonadal axis. A similar mechanism is proposed in sheep and mice, but the experimental data on the temporal patterns of induction and suppression of TSH-beta and Dio2 expression are incomplete. In the present study, we examined the expression of TSH-beta and Dio2 in hamsters transferred from short- to long-day conditions for 9 days, and demonstrate the induction of TSH-beta and Dio2 on day 8 after transition. These data demonstrate the close relationship between TSH-beta and Dio2 expression in the inductive pathway. The temporal expression of TSH-beta and Dio2 in the suppressive pathway was also examined by s.c. melatonin injection, which mimics the transition from long to short days. Importantly, Dio2 expression in the EC is suppressed on day 1 after the onset of injection, whereas TSH-beta expression in the PT was not suppressed until day 10. These data suggest that regulated transcription of TSH-beta is involved in the induction of the gonadal axis in mammals, whereas the suppression of this axis is mediated by different mechanisms.
Subject(s)
Hypothalamo-Hypophyseal System/metabolism , Photoperiod , Pituitary Gland, Anterior/metabolism , Thyrotropin, beta Subunit/metabolism , Analysis of Variance , Animals , Circadian Rhythm/physiology , Cricetinae , Ependyma/drug effects , Ependyma/metabolism , Fluorescent Antibody Technique , Hypothalamo-Hypophyseal System/drug effects , In Situ Hybridization , Iodide Peroxidase/genetics , Iodide Peroxidase/metabolism , Male , Melatonin/pharmacology , Mesocricetus , Pituitary Gland, Anterior/drug effects , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Thyrotropin/genetics , Receptors, Thyrotropin/metabolism , Third Ventricle/drug effects , Third Ventricle/metabolism , Thyrotropin, beta Subunit/genetics , Time Factors , Iodothyronine Deiodinase Type IIABSTRACT
Circadian rhythms are generated by an internal biological clock. The suprachiasmatic nucleus (SCN) in the hypothalamus is known to be the dominant biological clock regulating circadian rhythms in mammals. In birds, two nuclei, the so-called medial SCN (mSCN) and the visual SCN (vSCN), have both been proposed to be the avian SCN. However, it remains an unsettled question which nuclei are homologous to the mammalian SCN. We have identified circadian clock genes in Japanese quail and demonstrated that these genes are expressed in known circadian oscillators, the pineal and the retina. Here, we report that these clock genes are expressed in the mSCN but not in the vSCN in Japanese quail, Java sparrow, chicken, and pigeon. In addition, mSCN lesions eliminated or disorganized circadian rhythms of locomotor activity under constant dim light, but did not eliminate entrainment under light-dark (LD) cycles in pigeon. However, the lesioned birds became completely arrhythmic even under LD after the pineal and the eye were removed. These results indicate that the mSCN is a circadian oscillator in birds.
Subject(s)
Birds/physiology , Circadian Rhythm/physiology , Eye Proteins/genetics , Suprachiasmatic Nucleus/physiology , Animals , Biological Clocks , Chickens/physiology , Circadian Rhythm/genetics , Columbidae/physiology , Coturnix/physiology , Darkness , Light , Motor Activity , RNA, Messenger/analysis , RNA, Messenger/genetics , Songbirds/physiology , Transcription, GeneticABSTRACT
We investigated the characteristics of hemophilic arthropathy of the elbow joint in 32 patients 5-12 years of age who had been followed-up from infancy. Restriction of forearm rotation and elbow extension was the most common finding in the joints with recurrent hemarthrosis. Limitation of rotation resulted mainly from hypertrophy of the radial head. Advancement of bone age was also commonly observed in the joints with hemarthrosis. Mild to moderate hemophilic arthropathy was associated with greater advancement of bone age than was severe arthropathy. There was a discrepancy between the growth of the intraarticular subchondral epiphyseal cartilages and the cartilages of the medial and lateral epicondyles (apophyseal cartilage). This differential growth may be an important factor in the development of complicated and variable hemophilic elbow deformities.