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1.
Article in English | WPRIM (Western Pacific) | ID: wpr-1010297

ABSTRACT

OBJECTIVE@#To assess the outcomes after acupoint application in patients with pharyngeal pain in a real-world settings, and analyze the characteristics of effective population and prescription characteristics of acupoint application.@*METHODS@#Based on CHUNBO platform, patients with pharyngeal pain who were candidates for acupoint application on the basis of physician-evaluation, were enrolled in a nationwide, prospective, 69-week multicenter observational study from August 2020 to February 2022. Propensity score matching (PSM) was used to match the confounding factors and the association rules were used to analyze the characteristics of effective population and prescription characteristics of acupoint application. Outcome assessments included the disappearance rate of pharyngeal pain (within 3, 7, and 14 days), disappearance time of pharyngeal pain, as well as adverse events.@*RESULTS@#Of 7,699 enrolled participants, 6,693 (86.9%) received acupoint application and 1,450 (21.7%) with non-acupoint application. After PSM, there were 1,004 patients each in the application group (AG) and non-application group (NAG). The disappearance rate of pharyngeal pain in the AG at 3, 7, and 14 days were all higher than those in the NAG (P<0.05). The disappearance time of pharyngeal pain in the AG were shorter than that in the NAG (logrank P<0.001, hazard ratio=1.51, 95% confidence interval: 1.41-1.63). The median age of effective cases was 4 years, mainly 3-6 years old (40.21%). The disappearance rate of pharyngeal pain in the application group with tonsil diseases was 2.19 times higher than that in the NAG (P<0.05). The commonly used acupoints for the effective cases were Tiantu (RN 22), Shenque (RN 8) and Dazhui (DU 14). The commonly used herbs for the effective cases were Natrii sulfas, Radix et Rhizoma Rhei, and Herba Ephedrae. Among them, Natrii sulfas was applied to RN 8 most frequently (support 84.39%). A total of 1,324 (17.2%) patients experienced AEs, and mainly occurred in the AG, with significant difference in the incidence of AEs between goups (P<0.05). All AEs reported were the first grade, and the average regression days of AEs was 2.8 days.@*CONCLUSIONS@#Acupoint application in patients with pharyngeal pain resulted in improved effective rate and shortened duration, especially children aged 3-6 years old, and those with tonsil diseases. Acupoint of RN 22, RN 8 and DU 14, Natrii sulfas, Radix et Rhizoma Rhei, and Herba Ephedrae were the most commonly used herbs in the treatment of pharyngeal pain.


Subject(s)
Child , Humans , Child, Preschool , Acupuncture Points , Medicine, Chinese Traditional/methods , Prospective Studies , Pain
2.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-981526

ABSTRACT

This study aimed to evaluate the efficacy and safety of various oral Chinese patent medicines in the adjuvant treatment of rotavirus gastroenteritis(RVGE) in children based on network Meta-analysis. Randomized controlled trial(RCT) of oral Chinese patent medicine in the adjuvant treatment of RVGE in children was retrieved from the databases such as CNKI, Wanfang, VIP, SinoMed, PubMed, Cochrane Library, EMbase, and Web of Science from database inception to October 22, 2022. The quality of the included RCT was evaluated according to the Cochrane risk-of-bias tool, and the data were analyzed by RevMan 5.4 and Stata 16 software. Sixty-three RCTs were included, with 11 oral Chinese patent medicines involved, including Xingpi Yanger Granules, Weichang'an Pills, Qiuxieling Mixture, Erxieting Granules, and Changyanning Granules/Syrup. The results of the network Meta-analysis showed that in terms of clinical total effective rate, the top 3 optimal interventions were Changyanning Granules/Syrup, Xiaoer Guangpo Zhixie Oral Liquid, and Xiaoer Shuangjie Zhixie Granules combined with conventional western medicine. In terms of the anti-diarrheal time, the top 3 optimal interventions were Shenling Baizhu Granules, Qiuxieling Mixture, and Shuangling Zhixie Oral Liquid combined with conventional western medicine. In terms of the antiemetic time, the top 3 optimal interventions were Changyanning Granules/Syrup, Xingpi Yanger Granules, and Xiaoer Shuangjie Zhixie Granules combined with conventional western medicine. In terms of the antipyretic time, the top 3 optimal interventions were Shenling Baizhu Granules, Xiaoer Shuangjie Zhixie Granules, and Qiuxieling Mixture combined with conventional western medicine. In terms of the negative conversion rate of rotavirus, the top 3 optimal interventions were Xingpi Yanger Granules, Erxieting Granules, and Cangling Zhixie Oral Liquid combined with conventional western medicine. In terms of reducing creatine kinase isoenzyme MB(CK-MB) level, the top 3 optimal interventions were Weichang'an Pills, Xingpi Yanger Granules, and Xiaoer Shuangjie Zhixie Granules combined with conventional western medicine. In terms of adverse reactions, no se-rious adverse reactions were reported in all studies. Oral Chinese patent medicines in the adjuvant treatment of children with RVGE have their own advantages, Specifically, Changyanning Granules/Syrup + conventional western medicine focuses on improving the clinical total effective rate and shortening the antiemetic time, Shenling Baizhu Granules + conventional western medicine on shortening the anti-diarrheal time and antipyretic time, Xingpi Yanger Granules + conventional western medicine on improving the negative conversion rate of rotavirus, and Weichang'an Pills + conventional western medicine on reducing the CK-MB level. Limited by the quantity and quality of literature included in this study, the results need to be verified by high-quality RCT with a larger sample size.


Subject(s)
Child , Humans , Adjuvants, Pharmaceutic , Antiemetics , Antipyretics , Drugs, Chinese Herbal/therapeutic use , Enteritis/drug therapy , Network Meta-Analysis , Nonprescription Drugs/therapeutic use , Rotavirus , Randomized Controlled Trials as Topic
3.
Article in English | WPRIM (Western Pacific) | ID: wpr-970315

ABSTRACT

OBJECTIVE@#Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.@*METHODS@#In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.@*RESULTS@#Under optimal experimental conditions, the linear range of the sensor was 10 -13-10 -9 mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability.@*CONCLUSION@#The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.


Subject(s)
Humans , Microgels , Spike Glycoprotein, Coronavirus , COVID-19/diagnosis , Gold , Metal Nanoparticles , SARS-CoV-2
4.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-976117

ABSTRACT

@# Objective - - To analyze the prevalence and influencing factors of multi site work related musculoskeletal disorders ( ) Methods WMSDs in surgeons. A total of 102 surgeons from four hospitals were selected as study subjects by convenient sampling method. The Chinese version of Musculoskeletal Disorders Questionnaire was used to investigate the prevalence of , Results WMSDs in the past one year the related individuals and occupational factors. The total prevalence of WMSDs among ( ), ( ) ( ) surgeons was 54.9%. The top three sites were neck 48.0% lower back 35.3% and shoulder 32.4% . The prevalence of ( vs ,P ) WMSDs in multiple sites was higher than that in a single site 43.1% 11.8% <0.01 . Multivariate logistic regression , , analysis showed that surgeons who smoked were tired at work and had a bent back had a higher risk of developing WMSDs [ ( - ), ( - ), ( - ), P ] odds ratios and 95% confidence intervals were 3.66 1.41 9.46 8.33 2.15 32.20 and 18.74 2.14 166.77 all <0.01 Conclusion - after excluding the influence of confounding factors. The prevalence rate of multi site WMSDs among surgeons is , high and the influencing factors include bad living habits and occupational factors such as working load and working posture.

5.
Chinese Journal of Pediatrics ; (12): 227-231, 2022.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-935675

ABSTRACT

Objective: To investigate the clinical features, pathological phenotype, treatment and prognosis of Castleman's disease in children. Methods: Clinical data of 15 children diagnosed with Castleman's disease in Henan Provincial People's Hospital and the First Affiliated Hospital of Zhengzhou University from May 2010 to October 2019 were analyzed retrospectively. The clinical characteristics, laboratory examination and histopathological data were analyzed. Results: Among the 15 Castleman's disease patients, 12 were males and 3 females. The age of first visit was 12 (10, 15) years. The time from mass discovery to pathologic diagnosis was 9.0 (2.0, 13.0) months. The majority of patients were unicentric (13 cases), and the histopathological type was hyaline vascular (11 cases). Unicentric lesions were most common in the neck (11 cases), all 13 patients received complete surgical resection of the lesions, the follow-up time was 20.0 (13.5, 50.5) months, and the prognosis was good. Two cases were multicentric type, the pathological types were mixed variant, meeting the criteria of idiopathic Castleman's disease, the two children underwent partial surgical resection, one was treated with rituximab and prednisone and the other was treated with thalidomide and prednisone. The follow-up time was 32 months and 10 month, both of them had good prognosis. Conclusions: Most cases of Castleman's disease in children are diagnosed late, and the unicentric type is dominant. The most common pathological type is hyaline vascular, which is characterized by painless lymphadenopathy, while multicentric type has systemic symptoms and both of them have a good overall prognosis.


Subject(s)
Female , Humans , Male , Castleman Disease/therapy , Neck/pathology , Prognosis , Retrospective Studies , Rituximab
6.
Preprint in English | medRxiv | ID: ppmedrxiv-21254429

ABSTRACT

Predictively forecasting future developments for the spread of the COVID-19 pandemic is extremely challenging. A recently published logistic mathematic model has achieved good predictions for infections weeks ahead. In this short communication, we summarize the Logistic spread model, which describes the dynamics of the pandemic evolution and the impacts of people social behavior in fighting against the pandemic. The new pandemic model has two parameters (i.e., transmission rate {gamma} and social distancing d) to be calibrated to the data from the pandemic regions in the early stage of the outbreak while the social distancing is put in place. The model is capable to make early predictions about the spreading trajectory in the communities of any size (countries, states, counties and cities) including the total infections, the date of peak daily infections and the date of the infections reaching a plateau if the testing is sufficient. The results are in good agreement with data and have important applications for ongoing outbreaks and similar infectious disease pandemics in the future.

7.
Acta Anatomica Sinica ; (6): 465-472, 2021.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-1015467

ABSTRACT

Objective To investigate the somatotype characteristics of Dong adults in rural areas of Hu'nan province. Methods The Heath-Carter somatotyping method was used to study the somatotype of 749 adults (304 males and 445 females) of Dong in the rural areas of Hu'nan. Results The mean somatotype in males of Dong was endomorph- mesomorph category (4.83-4.95-1.80) and was mesomorphic endomorph category (6.09-4.68-1.37) in females. With their age increasing, the values of endomorphy decreases gradually in males, the values of endomorphy of females and mesomorphy of males and females increased at first and then decreased gradually, the values of ectomorphy of males and females decreased at first and then increased. The values of endomorphy of males were significantly lower than those of females with the same age groups, and the values of mesomorphy and the values of ectomorphy of males were significantly higher than those of females in some age groups. Compared with other ethnic groups, the values of endomorphy of Dong were larger, the values of mesomorphy were smaller, and the values of ectomorphy were in a middle level. Conclusion Adults of Dong in Hu'nan have thick subcutaneous fat, underdeveloped skeletal and muscular systems, and medium linearity. The somatotype of Dong adults in Hu'nan is close to that of Jiangsu Han, Fujian Han and Liaoning Han.

8.
Acta Pharmaceutica Sinica ; (12): 456-464, 2021.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-873773

ABSTRACT

Biomarkers are defined as a characteristic that is objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, or pharmacological responses to a therapeutic intervention. Biomarkers can help the decision-making process for new drug research and development, provide guidance for the early clinical development of candidate drugs and reduce the risk of failure. Therefore, as a key factor in the development of new drugs, the discovery and research on biomarkers has increased the interest of the pharmaceutical industry and regulatory agencies. Guidelines on the development and use of biomarkers have been issued by drug regulatory agencies including the EMA, FDA and ICH. Biomarkers are encouraged to be used to facilitate drug development by these relevant regulatory agencies, and also to be used to monitor the safety and efficacy of drugs in post-marketing drug surveillance. The application of biomarkers is encouraged at different stages of a drug's life cycle, including at the stage of basic science research and target identification, prototype design or discovery, preclinical development, clinical development, FDA filling/approval and launch, as well as post-marketing was reviewed. The identification, development, and application of biomarkers in pharmaceutical research is discussed.

9.
Acta Pharmaceutica Sinica ; (12): 1921-1926, 2021.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-887006

ABSTRACT

In this study, a rat morphine drug discrimination model with a fixed ratio (FR) of 10 (FR10) was established using different methods to explore which methods can shorten the modeling time and test the dose-response relationship and median effective dose (ED50) value. Animal welfare and experimental procedures are in accordance with the provision of the Animal Ethics Committee of Shanghai InnoStar Bio-tech Co., Ltd. Forty rats were initially shaped to press lever under a fixed-ratio schedule of food reinforcement. The animals that were successfully trained under a FR10 schedule of food reinforcement were divided into two groups, namely the single-lever + double-lever training group 1 and the double-lever training group 2. In each group, rats were trained to discriminate morphine at 5.6 mg·kg-1 from saline by the intraperitoneal route. After training, different doses of morphine were used to substitute for training dose of morphine, the dose-response curve for morphine were identified in rats, and the ED50 value was calculated. The results showed that, in food training phase: 34 rats successfully entered the discrimination training during food training; in discrimination training phase: 14 animals in group 1 met the discrimination training standard for the first time, which took about (40.71 ± 2.93) days, and there were 13 animals in group 2 that met the discrimination training criteria for the first time, and it took about (51.15 ± 2.55) days. It can be seen that the method of single-lever + double-lever training is better than single-lever training, and the difference is significant compared with group 1 (P ˂ 0.05); in generalization test phase: there are 17 rats completed morphine generalization test, and the percentages of morphine-lever responses produced by the generalization test of different doses of morphine (0, 0.1, 0.5, 1, 3, 5.6, and 10 mg·kg-1) were (9.56 ± 3.13) %, (9.01 ± 5.83) %, (13.82 ± 7.95) %, (29.04 ± 10.13) %, (41.70 ± 10.65) %, (85.36 ± 7.16) %, (94.56 ± 2.76) %, respectively. The results showed that the discriminative stimulative effect induced by morphine dose between 0-10 mg·kg-1 increased in a dose-dependent manner, producing a good dose-response curve, and the ED50 value of morphine was 4.74 mg·kg-1 by linear fitting. The above results showed that, the FR10 morphine drug discrimination model has been successfully established using different methods; the single-lever + double-lever training method is better than the single-lever training, and can relatively shorten the discrimination training cycle.

10.
Preprint in English | medRxiv | ID: ppmedrxiv-20166967

ABSTRACT

This work presents a mathematical model that captures time-dependent social-distancing effects and presents examples of the consequences of relaxing social-distancing restrictions in the fight against the novel coronavirus epidemic. Without social distancing, the spread of COVID-19 will grow exponentially, but social distancing and peoples learning behavior (isolating, staying at home, wearing face masks, washing hands, restricting the size and frequency of group gatherings, etc.) can significantly impede the epidemic spread, flatten the infection curve, and change the final outcome of the COVID-19 outbreak. Our results demonstrate that strict social distancing and peoples learning behavior can be effective in slowing the spread rate and significantly reducing the total number of infections, daily infection rate, peak of daily infections, and duration of the epidemic. Under strict social distancing, the rise and fall of infections would be nearly symmetric about the peak of of daily infections, and the epidemic spread would be essentially over within 60 days. Relaxing social distancing and people learning behaviors will significantly increase the total and daily numbers of infections and prolong the course of the outbreak. These results have immediate applications for the implementation of various social-distancing policies and general significance for ongoing outbreaks and similar infectious disease epidemics in the future (LA-UR 20-22877). DisclaimerThis material is not final and is subject to be updated any time. Contact information: bcheng@lanl.gov.)

11.
Preprint in English | medRxiv | ID: ppmedrxiv-20081281

ABSTRACT

The spread of the novel coronavirus is characterized by two phases: (I) a natural exponential growth phase that occurs in the absence of intervention and (II) a regulated growth phase that is affected by enforcing social distancing and isolation. We have developed a fundamental spreading model for the COVID-19 epidemic that has two parameters: the community transmission rate and a metric describing the degree of isolation and social distancing in a given community or region (country, state, county, or city). These two parameters are calibrated to data from the community, so the model uncertainty depends on the quality of the data and ability to test for COVID-19. The model shows that social distancing significantly reduces the epidemic spread and flattens the curve. The model predicts well the spreading trajectory and peak time of new infections for a community of any size and provides an upper estimate for the total number of infections and daily new infection rate for weeks into the future, providing the vital information and lead time needed to prepare for and mitigate the epidemic. The theory has immediate and far-reaching applications for ongoing outbreaks or similar future outbreaks of other emergent infectious diseases (LA-UR-20-22877). DisclaimerThis material is not final and is subject to be updated any time. Contact information: bcheng@lanl.gov.

12.
Journal of Medical Postgraduates ; (12): 377-380, 2018.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-700837

ABSTRACT

Objective At present, there is little report of clinical results of metal-on-metal HRA in China.This study was aimed at retrospectively analyzing the factors of survival rate of HRA in China. Methods A retrospective study was conducted in 113 patients who accepted HRA in our hospital from September 2005 to May 2010.All patients were followed up by telephone or outpatient visit,and the average follow-up time was 10.2 years.The clinical function was evaluated by Harris score,and the prosthesis was evalu-ated by pelvic film.Inductively coupled plasma mass spectrometry(ICP-MS)was used to detect the concentration of blood metal ions, ultrasound and MRI were used to screen and diagnose inflammatory pseudotumor.Cox proportional hazards models were used to analyze the factors affecting the survival rate of the prosthesis. Results A total of 91 patients(120 hips)were followed up while 22 patients (26 hips)were lost,the follow-up rate was 80.5%.17 patients underwent revision surgeries,including 8 cases of femoral neck fracture and 8 cases of aseptic loosening,the other one was infection.Revision in 13 cases was within 3 years, the proportion was as high as 76.5%.The 10-year survival rate of prosthesis was 87.1%,the abduction angle of acetabular prosthesis is the only risk factor affecting the survival. Conclusion Postoperative survival rate of HRA is relatively high, and the survival rate of prosthesis is relevant to socket position.We should notice that the risk increased when the acetabular abduction angle of the prosthesis was over 55 degree.O-verall,HRA is still a successful surgical method.

13.
Acta Pharmaceutica Sinica ; (12): 1827-1838, 2017.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-779795

ABSTRACT

Cell membrane chromatography (CMC) was first proposed by Professor He in 1996. As one of bio-affinity chromatography technique, CMC was a simple and convenient technology in the study of interactions of active components in traditional Chinese medicines (TCMs) with membrane receptors in vitro, and screen active components from complicated TCMs. Recently, the CMC technology was developed rapidly, and widely applied in the discovery of lead compounds from nature product. This review article is focused on the application of cell membrane chromatography in the identification of active components in traditional Chinese medicine, together with the recent development of CMC methodology. Combining with our previous works in the analysis of the composition of complex substances, biochromatography and TLC bioautography for quality evaluation of TCM, we proposed a new holistic quality evaluation strategy of TCM related with bioactivity, which could be summarized as the integration of screen (screening of quality control marker by CMC), macroscopic characterization (characterizing the chemical material basis by multi-dimension and multi data fingerprinting) and microscopic description (multi-component quantification). The proposed strategy would provide a new idea for the holistic quality evaluation of TCM in the composition and concentration of bioactive components as quality evaluation indicators.

14.
Acta Pharmaceutica Sinica ; (12): 556-562, 2017.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-779628

ABSTRACT

This study was aimed to construct a “drug-target-pathway” network of Xuebijing injection for sepsis treatment in an effort to explore the “multi-components, multi-targets, multi-pathways” mechanism based on “system-system” research mode. Active ingredients of Xuebijing injection were used to predict the potential targets according to reversed pharmacophore matching method. The pathway information was acquired from DAVID and KEGG database. The Cytoscape software was used to construct the “ingredient-target-pathway” network of Xuebijing injection for sepsis treatment. The results showed that 21 major active ingredients of Xuebijing injection regulated 550 targets (HRAS, GSK3B, BTK, AK, et al) and affected 10 inflammation and immune-related pathways, such as B cell receptor signaling pathway, VEGF signaling pathway, natural killer cell mediated cytotoxicity, Toll-like receptor signaling pathway. The sepsis therapeutic effect of Xuebijing injection reflected the action features of traditional Chinese medicine as multi-ingredients, multi-targets, multi-pathways. This research clarifies the material basis of Xuebijing injection for anti-inflammation and immunoregulation, providing a scientific basis for elucidation the mechanism of Xuebijing injection in the treatment of sepsis.

15.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-230947

ABSTRACT

To observe the effect of processed Polygonum multiflorum on mRNA expression levels of five subtypes of CYP450 enzymes in rat liver. SD rats were randomly divided into the normal control group, processed P. multiflorum high dose and low dose groups (5.40 g•kg⁻¹ and 1.08 g•kg⁻¹). The rats in administration groups were continuously given with processed P. mutiflorum for 7 days by ig administration, and the rats in normal control group were given with the same volume of distilled water. After successive administration of 7 days, the serum biochemical indications were detected, and Real-time quantitative PCR technology was used to detect the mRNA expression levels of five subtypes of CYP450 enzymes in rat liver. Experimental results showed that AST was decreased significantly in both low and high dose groups. ALT was significantly decreased in low dose group and significantly increased in high dose group. The mRNA expression levels of five subtypes of CYP450 enzymes in rat liver were decreased in high dose and low dose groups in a dose-dependent manner. Especially the high dose processed P. multiflorum could significantly inhibit CYP1A2 and CYP2E1 mRNA expression levels in rats. The study showed that high dose P. multiflorum water extract had hepatotoxicity, and the degree of liver damage was increased with the increase of dose. It shall be noted that 5.40 g•kg⁻¹ water extract of P. multiflorum could significantly inhibit CYP1A2 and CYP2E1 mRNA expression levels in the liver of rats.

16.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-271912

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinical efficacy of cellular immunotherapy combined with bortezomib for treatment of patients with multiple myeloma.</p><p><b>METHODS</b>A total of 76 patients with multiple myeloma in our hospital from October 2012 to October 2013 were selected and randomly divided into 2 groups: the patients in 1 group (38 cases) were treated with cellular immunotherapy combined with chemotherapy including bortezomib (combined therapy group), the patients in other group(38 cases) were treated with only chemotherapy including bortezomib(single chemotherapy as control group). The treatment remission rate, the expression changes of immunophenotype, progression-free survival(PFS) and adverse reactions were compared in the 2 groups.</p><p><b>RESULTS</b>The total remission rate of combined therapy group was significantly higher than that in the control group (P<0.05); the positive rates of CD38, CD56and CD138in combined therapy group were all significantly lower than those in control group, and the CD19was significantly higher (P<0.05). The PFS rates of 1, 2 and 3 years in the combined therapy group were all significantly higher than those in the control group (P<0.05). The incidence of fatigue, rash, peripheral neuropathy, anemia and granulocyte deficiency in the combined therapy group was all significantly lower than that in the control group (P<0.05).</p><p><b>CONCLUSION</b>Cellular immunotherapy combined with bortezomib can significantly improve the remission rate, prolong survival, and significantly decrease adverse event rate of multiple myeloma patients.</p>

17.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-256532

ABSTRACT

<p><b>OBJECTIVE</b>To detect the expressions of cyclooxygenase-2 (COX-2) and basic fibroblast growth factor (bFGF) and evaluate their value in predicting the radiotherapy sensitivity in nasopharyngeal carcinoma (NPC).</p><p><b>METHODS</b>The expressions of COX-2 and bFGF were detected immunohistochemically in biopsy samples of NPC, and their relationship with the radiotherapy sensitivity of the tumors were analyzed.</p><p><b>RESULTS</b>In 97 NPC cases, the positivity rates of COX-2 and bFGF were 71.1% (69/97) and 64.9% (63/97), respectively. Correlation analysis demonstrated that a positive COX-2 expression was positively correlated with an advanced T status and N status, and bFGF expression was positively associated with an advanced N status in NPCs. In radiotherapy-sensitive and radiotherapy-insensitive cases, the positive rate of COX-2 was 62.8% and 92.6%, and that of bFGF was 57.1% and 85.2%, respectively. The expression of COX-2 was positively correlated with that of bFGF (r=0.486, P<0.05). The radiotherapy sensitivity differed significantly among patients with different statuses of COX-2 and bFGF positivity.</p><p><b>CONCLUSION</b>COX-2 and bFGF can be effective and sensitive biomarkers for predicting radiotherapy sensitivity in NPC.</p>

18.
Acta Pharmaceutica Sinica ; (12): 284-290, 2015.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-251782

ABSTRACT

Applications of network pharmacology are increasingly widespread and methods abound in the field of drug development and pharmacological research. In this study, we choose rosiglitazone compound as the object to predict the targets and to discuss the mechanism based on three kinds of prediction methods of network pharmacology. Comparison of the prediction result has identified that the three kinds of prediction methods had their own characteristics: targets and pathways predicted were not in accordance with each other. However, the calcium signaling pathway could be predicted in the three kinds of methods, which associated with diabetes and cognitive impairment caused by diabetes by bioinformatics analysis. The above conclusion indicates that the calcium signaling pathway is important in signal pathway regulation of rosiglitazone compound, which provides a clue to further explain the mechanism of the compound and also provides a reference for the selection and application of methods of network pharmacology in the actual research.


Subject(s)
Humans , Calcium Signaling , Cognitive Dysfunction , Computational Biology , Diabetes Mellitus , Pharmacology , Methods , Thiazolidinediones , Pharmacology
19.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-300157

ABSTRACT

In this study, the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis, and explore its possible mechanism. The cell model was established to determine the effect of lipophilic components in S. miltiorrhiza on the proliferative activity and migration capacity of endothelial cells. Then the realtime fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cytokines VEGF-A, VEGF-C and MMP-9. The results showed that 5 mg x L(-1) lipophilic components in S. miltiorrhiza could inhibit the proliferation and migration of endothelial cells, and reduce the expression of VEGF-A and MMP-9 genes. It indicated that lipophilic components in S. miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes, so as to show the inhibitory effect on angiogenesis.


Subject(s)
Humans , Angiogenesis Inhibitors , Chemistry , Pharmacology , Cell Movement , Cell Proliferation , Drugs, Chinese Herbal , Chemistry , Pharmacology , Human Umbilical Vein Endothelial Cells , Cell Biology , Metabolism , Matrix Metalloproteinase 9 , Genetics , Metabolism , Salvia miltiorrhiza , Chemistry , Vascular Endothelial Growth Factor A , Genetics , Metabolism
20.
Acta Pharmaceutica Sinica ; (12): 1415-1421, 2013.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-259460

ABSTRACT

The objective of this study is to fully investigate the therapeutic effect and mechanisms of action of Gegen Qinlian decoction (GD) on type 2 diabetes mellitus (type 2 DM). A rat model of type 2 DM was established with the combination of high-fat diet and multiple low doses of streptozotocin (STZ). Biochemical indicators related to glucose metabolism disorders, insulin resistance, oxidative stress were observed. The type 2 DM rats were administrated with GD for 80 days, the above-mentioned indexes were detected. The results indicated that the hepatic glycogen synthesis level was promoted, fasting blood glucose level and fasting blood insulin level were significantly reduced, insulin sensitivity index was significantly improved; the level of superoxide dismutase (SOD) was increased and the level of malondialdehyde (MDA) was reduced; pathologic morphology of pancreas and kidney was ameliorated in the GD group. It was indicated that the therapeutic mechanisms of action of GD on type 2 DM might be related to its effect of ameliorating glucose metabolism disorders, relieving insulin resistance, increasing the tissues' sensitivity to insulin, improving the antioxidative ability of living system, GD has therapeutic effect on type 2 DM and protective effects against damaged pancreatic function.


Subject(s)
Animals , Female , Male , Rats , Blood Glucose , Metabolism , Diabetes Mellitus, Type 2 , Drug Therapy , Metabolism , Pathology , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Glycogen , Metabolism , Hypoglycemic Agents , Pharmacology , Therapeutic Uses , Insulin , Blood , Insulin Resistance , Kidney , Pathology , Liver , Metabolism , Malondialdehyde , Metabolism , Pancreas , Pathology , Phytotherapy , Plants, Medicinal , Chemistry , Random Allocation , Rats, Sprague-Dawley , Streptozocin , Superoxide Dismutase , Metabolism
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