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1.
Redox Biol ; 72: 103128, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38554523

ABSTRACT

YbbN/CnoX are proteins that display a Thioredoxin (Trx) domain linked to a tetratricopeptide domain. YbbN from Escherichia coli (EcYbbN) displays a co-chaperone (holdase) activity that is induced by HOCl. Here, we compared EcYbbN with YbbN proteins from Xylella fastidiosa (XfYbbN) and from Pseudomonas aeruginosa (PaYbbN). EcYbbN presents a redox active Cys residue at Trx domain (Cys63), 24 residues away from SQHC motif (SQHC[N24]C) that can form mixed disulfides with target proteins. In contrast, XfYbbN and PaYbbN present two Cys residues in the CXXC (CAPC) motif, while only PaYbbN shows the Cys residue equivalent to Cys63 of EcYbbN. Our phylogenetic analysis revealed that most of the YbbN proteins are in the bacteria domain of life and that their members can be divided into four groups according to the conserved Cys residues. EcYbbN (SQHC[N24]C), XfYbbN (CAPC[N24]V) and PaYbbN (CAPC[N24]C) are representatives of three sub-families. In contrast to EcYbbN, both XfYbbN and PaYbbN: (1) reduced an artificial disulfide (DTNB) and (2) supported the peroxidase activity of Peroxiredoxin Q from X. fastidiosa, suggesting that these proteins might function similarly to the canonical Trx enzymes. Indeed, XfYbbN was reduced by XfTrx reductase with a high catalytic efficiency (kcat/Km = 1.27 x 107 M-1 s-1), similar to the canonical XfTrx (XfTsnC). Furthermore, EcYbbN and XfYbbN, but not PaYbbN displayed HOCl-induced holdase activity. Remarkably, EcYbbN gained disulfide reductase activity while lost the HOCl-activated chaperone function, when the SQHC was replaced by CQHC. In contrast, the XfYbbN CAPA mutant lost the disulfide reductase activity, while kept its HOCl-induced chaperone function. In all cases, the induction of the holdase activity was accompanied by YbbN oligomerization. Finally, we showed that deletion of ybbN gene did not render in P. aeruginosa more sensitive stressful treatments. Therefore, YbbN/CnoX proteins display distinct properties, depending on the presence of the three conserved Cys residues.


Subject(s)
Escherichia coli , Oxidoreductases , Pseudomonas aeruginosa , Thioredoxins , Xylella , Amino Acid Sequence , Bacterial Proteins/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/chemistry , Escherichia coli/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/metabolism , Escherichia coli Proteins/genetics , Escherichia coli Proteins/chemistry , Oxidation-Reduction , Oxidoreductases/metabolism , Oxidoreductases/genetics , Oxidoreductases/chemistry , Phylogeny , Pseudomonas aeruginosa/enzymology , Pseudomonas aeruginosa/genetics , Pseudomonas aeruginosa/metabolism , Thioredoxins/metabolism , Thioredoxins/genetics , Thioredoxins/chemistry , Xylella/enzymology , Xylella/genetics , Xylella/metabolism
2.
Exp Gerontol ; 58: 120-7, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25084214

ABSTRACT

The p53 protein, a transcription factor with many gene targets, can also trigger apoptosis in the cytoplasm. The disruption of cell homeostasis, such as Ca(2+) signaling and mitochondrial respiration, contributes to the loss of viability and ultimately leads to cell death. However, the link between Ca(2+) signaling and p53 signaling remains unclear. During aging, there are alterations in cell physiology that are commonly associated with a reduced adaptive stress response, thus increasing cell vulnerability. In this work, we examined the effects of a cytoplasmic p53 inhibitor (pifithrin µ) in the striatum of young and aged rats by evaluating Ca(2+) signaling, mitochondrial respiration, apoptotic protein expression, and tissue viability. Our results showed that pifithrin µ differentially modulated cytoplasmic and mitochondrial Ca(2+) in young and aged rats. Cytoplasmic p53 inhibition appeared to reduce the mitochondrial respiration rate in both groups. In addition, p53 phosphorylation and Bax protein levels were elevated upon cytoplasmic p53 inhibition and could contribute to the reduction of tissue viability. Following glutamate challenge, pifithrin µ improved cell viability in aged tissue, reduced reactive oxygen species (ROS) generation, and reduced mitochondrial membrane potential (ΔΨm). Taken together, these results indicate that cytoplasmic p53 may have a special role in cell viability by influencing cellular Ca(2+) homeostasis and respiration and may produce differential effects in the striatum of young and aged rats.


Subject(s)
Aging/metabolism , Basal Ganglia/drug effects , Calcium Signaling/drug effects , Cell Survival/drug effects , Cytoplasm/metabolism , Mitochondria/drug effects , Sulfonamides/pharmacology , Tumor Suppressor Protein p53/antagonists & inhibitors , Age Factors , Aging/pathology , Animals , Basal Ganglia/metabolism , Basal Ganglia/pathology , Basal Ganglia/radiation effects , Calcium Signaling/radiation effects , Cell Survival/radiation effects , Glutamic Acid/toxicity , Glutathione/metabolism , Homeostasis , In Vitro Techniques , Membrane Potential, Mitochondrial/drug effects , Mitochondria/metabolism , Mitochondria/radiation effects , Oxidative Stress/drug effects , Phosphorylation , Rats, Wistar , Reactive Oxygen Species/metabolism , Tumor Suppressor Protein p53/metabolism , Ultraviolet Rays , bcl-2-Associated X Protein/metabolism
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