ABSTRACT
Treatment with a combination of peripheral nerve grafts and acidic fibroblast growth factor improves hind limb locomotor function after spinal cord transection. This study examined the effect of treatment on expression of arginase I (Arg I) and polyamines. Arg I expression was low in the spinal cords of normal rats but increased following spinal injury. Only fully repaired spinal cords expressed higher Arg I levels 6-14 days following repair. In 10-day repaired spinal cords, high Arg I immunoreactivity was detected in motoneurons and alternatively activated macrophages in the graft area and graft-stump edges, and high levels of the polyamine spermine were expressed by macrophages within the intercostal nerve graft. Thus, in addition to enhancing the expression of Arg I and spermine in repaired spinal cords, our treatment may recruit activated macrophages and create a more favorable environment for axonal regrowth.
Subject(s)
Arginase/metabolism , Carrier Proteins/administration & dosage , Peripheral Nerves/transplantation , Polyamines/metabolism , Spermine/metabolism , Spinal Cord Injuries/metabolism , Spinal Cord Injuries/surgery , Animals , Female , Membrane Proteins , Nerve Regeneration/drug effects , Nerve Regeneration/physiology , Rats , Rats, Sprague-Dawley , Spinal Cord/drug effects , Spinal Cord/metabolism , Spinal Cord/surgery , Spinal Cord Injuries/drug therapyABSTRACT
OBJECTIVE: To establish the quality control standard for Xueyakang capsule. METHOD: Radix Rehmanniae, Cacumen Platycladi, Folium Artemisiae Argyi, Folium Nelumbinis were identified by TLC, and the content of quercitrin was determined by HPLC. RESULT: The TLC sports developed was fairly clear, the HPLC method showed good repeatability, and the average recovery of quercitrin was 100.7% with RSD 2.0%. CONCLUSION: The method is simple, accurate and can effectively control quality of Xueyakang capsule.