Insight into spatial variations of DOM fractions and its interactions with microbial communities of shallow groundwater in a mesoscale lowland river watershed.

Dissolved organic matter (DOM) plays a crucial role in driving biogeochemical processes and determining water quality in shallow groundwater systems, where DOM could be susceptible to dynamic influences of surface water influx. This study employed fluorescence excitation-emission matrix (EEM) spectroscopy combined with principal component coefficients, parallel factor analysis (PARAFAC), co-occurrence network analysis and structural equation modeling (SEM) to examine changes of DOM fractions from surface water to shallow groundwater in a mesoscale lowland river basin. Combining stable isotope and hydrochemical parameters, except for surface water (SW), two groups of groundwater samples were defined, namely, deeply influenced by surface water (IGW) and groundwater nearly non-influenced by surface water (UGW), which were 50.34 % and 19.39 % recharged by surface water, respectively. According to principal component coefficients, reassembled EEM data of these categories highlighted variations of the tyrosine-like peak in DOM. EEMs coupled with PARAFAC extracted five components (C1-C5), i.e. C1, protein-like substances, C2 and C4, humic-like substances, and C3 and C5, microbial-related substances. The abundance of the protein-like was SW > IGW > UGW, while the order of the humic-like was opposite. The bacterial communities exhibited an obvious cluster across three regions, which hinted their sensitivity to variations in environmental conditions. Based on co-occurrence, SW represented the highest connectivity between bacterial OTUs and DOM fractions, followed by IGW and UGW. SEM revealed that microbial activities increased bioavailability of the humic-like in the SW and IGW, whereas microbial compositions promoted the evolution of humic-like substances in the UGW. Generally, these results could be conducive to discern dissimilarity in DOM fractions across surface water and shallow groundwater, and further trace their interactions in the river watershed.

Appling fluorescence spectroscopy with absolute principal component coefficient to explore dynamic migration of DOM fractions from an urbanized river during torrential rainfall.

This research delves into the dynamic interplay between urbanization and the characteristics of Dissolved Organic Matter (DOM) in the Anyang River, particularly under the stress of torrential rain. The motivation stems from a critical need to decipher how urban landscapes influence water quality, focusing on the intricate transformations and movements of DOM. Employing advanced fluorescence spectroscopy techniques like Excitation-Emission Matrices (EEM) and Parallel Factor Analysis (PARAFAC), the study meticulously differentiates DOM compositions in urban and agricultural settings. It unveils a pronounced distinction, with urban streams showing elevated proteinaceous DOM from wastewater, contrasting with the humic substances prevalent in agricultural runoff. The analysis also captures how intense rainfall events catalyze significant shifts in DOM profiles, thereby emphasizing the need for tailored water quality management strategies in urbanized catchments. This comprehensive approach not only bridges gaps in understanding the urban impact on riverine ecosystems but also sets a foundation for future research and policy development in the face of escalating environmental changes.

Applying fluorescence spectroscopy coupled with Gaussian band fitting to reveal dynamic variation process of humus fractions from riparian soils along an urbanized river.

Humus, an important fraction of soil organic matter, play an environmental role on nutrients, organic and inorganic pollutants in riparian zones of urbanized rivers. In this study, dynamic variation process of humus fractions from riparian soils was revealed along Puhe River. Composite soil samples of four depths were collected from four land-uses, i.e., eco-conservation area (ECA), industrial area (INA), urban/town area (UTA), rural/agricultural area (RAA). Based on synchronous fluorescence spectra coupled with Gaussian band fitting, fulvic/humic acid predominantly contained tyrosine-like (TYLF), tryptophan-like (TRLF), microbial-like (MLF), fulvic-like (FLF) and humic-like (HLF) substances within each soil profile. TRLF, MLF and FLF (89.43-90.30 %) are the representative components in fulvic-acid, while MLF and HLF (52.81-59.97 %) in humic-acid. Phenolic, carboxylic and humified materials were present in both humus. According to 2-dimensitonal correlation spectroscopy and canonical correlation analysis, fulvic/humic acid within the ECA soil profile could be mainly derived from the degradations of terrestrial plant metabolites and residuals. Within the INA, fulvic-acid could be associated with treated/untreated wastewater, which entered the river and flew into the riparian during high flow period; whereas humic-acid could be relative to the terrestrials. Fulvic-acid had the same source as humic-acid in the UTA, which might be concerned with scattered domestic sewage and livestock wastewater, rather than the fluvial water. Furthermore, the source of fulvic/humic acid in the RAA was the crop metabolites and residuals, apart from the livestock wastewater. Noticeably, the variations of humus fractions in the ECA and RAA roughly occurred in 0-60 cm, while approximately in 20-80 cm in the INA and UTA. This proved that humus fractions in the former were referred to the plant/crop residuals, whereas humus fractions in the latter were those the terrestrials and fluvial water. This study could provide a key support for the construction and restoration of the urbanized riparian zone.

Mitigated N2O emissions from submerged-plant-covered aquatic ecosystems on the Changjiang River Delta.

Submerged plants affect nitrogen cycling in aquatic ecosystems. However, whether and how submerged plants change nitrous oxide (N2O) production mechanism and emissions flux remains controversial. Current research primarily focuses on the feedback from N2O release to variation of substrate level and microbial communities. It is deficient in connecting the relative contribution of individual N2O production processes (i.e., the N2O partition). Here, we attempted to offer a comprehensive understanding of the N2O mitigation mechanism in aquatic ecosystems on the Changjiang River Delta according to stable isotopic techniques, metagenome-assembly genome analysis, and statistical analysis. We found that the submerged plant reduced 45 % of N2O emissions by slowing down the dissolved inorganic nitrogen conversion velocity to N2O in sediment (Vf-[DIN]sed). It was attributed to changing the N2O partition and suppressing the potential capacity of net N2O production (i.e., nor/nosZ). The dominated production processes showed a shift with increasing excess N2O. Meanwhile, distinct shift thresholds of planted and unplanted habitats reflected different mechanisms of stimulated N2O production. The hotspot zone of N2O production corresponded to high nor/nosZ and unsaturated oxygen (O2) in unplanted habitat. In contrast, planted habitat hotspot has lower nor/nosZ and supersaturated O2. O2 from photosynthesis critically impacted the activities of N2O producers and consumers. In summary, the presence of submerged plants is beneficial to mitigate N2O emissions from aquatic ecosystems.

Differences in greenhouse gas emissions and microbial communities between underground and conventionally constructed wastewater treatment plants.

Large quantities of greenhouse gases (GHGs) are emitted into the atmosphere during wastewater treatment. In this study, GHG and microbial samples were collected from four wastewater treatment plants (WWTPs), and their differences and relationships were assessed. The study showed that, compared with conventionally constructed WWTPs, well-established gas collection systems in underground WWTPs facilitate comprehensive collection and accurate accounting of GHGs. In aboveground WWTPs, capped anoxic ponds promote methane production releasing it at 2-8 times the rate of uncapped emissions, in contrast to nitrous oxide emissions. Moreover, a stable subsurface environment allows for smaller fluctuations in daily GHG emissions and higher microbial diversity and abundance. This study highlights differences in GHG emission fluxes and microbial communities in differently constructed WWTPs, which are useful for control and accurate accounting of GHG emissions.

Novel hemoperfusion adsorbents based on collagen for efficient bilirubin removal - A thought from yellow skin of patients with hyperbilirubinemia.

Hemoperfusion is a well-developed method for removing bilirubin from patients with hyperbilirubinemia. The performance of adsorbents is crucial during the process. However, most adsorbents used for bilirubin removal are not suitable for clinical applications, because they either have poor adsorption performance or limited biocompatibility. Patients with hyperbilirubinemia usually have distinctive yellow skin, indicating that collagen, a primary component of the skin, may be an effective material for absorbing bilirubin from the blood. Based on this idea, we designed and synthesized collagen (Col) and collagen-polyethyleneimine (Col-PEI) microspheres and employed them as hemoperfusion adsorbents for bilirubin removal. The microspheres have an efficient adsorption rate, higher bilirubin adsorption capacity, and competitive adsorption of bilirubin in the bilirubin/bovine serum albumin (BSA) solution. The maximum adsorption capacities of Col and Col-PEI microspheres for bilirubin are 150.2 mg/g and 258.4 mg/g, respectively, which are higher than those of most traditional polymer microspheres. Additionally, the microspheres exhibit excellent blood compatibility originating from collagen. Our study provides a new collagen-based strategy for the hemoperfusion treatment of hyperbilirubinemia.

Characterizing dissolved organic matter and bacterial community interactions in a river network under anthropogenic landcover.

Anthropogenic landcover could rise nutrient concentrations and impact the characteristics and bioavailability of dissolved organic matter (DOM) in a river network. Exploring the interactions between DOM and microbials might be conducive to revealing biogeochemistry behaviors of organic matter. In this study, synchronous fluorescence spectra (SFS) with Gaussian band fitting and two-dimensional correlation spectroscopy (2D-COS) were employed to identify DOM fractions and reveal their interactions with bacterial communities. DOM was extracted from a river network under eco-agricultural rural (RUR), eco-residential urban (URB), eco-economical town (TOW), and eco-industrial park (IND) regions in Jiashan Plain of eastern China. The overlapping peaks observed in the SFS were successfully separated into four fractions using Gaussian band fitting, i.e., tyrosine-like fluorescence (TYLF), tryptophan-like fluorescence (TRLF), microbial humic-like fluorescence (MHLF), and fulvic-like fluorescence (FLF) materials. Across all four regions, TRLF (44.79% ± 7.74%) and TYLF (48.09% ± 8.85%) were the dominant components. Based on 2D-COS, variations of TYLF and TRLF were extremely larger than those of FLF in RUR-TOW. However, in URB-IND, the former exhibited lower variations compared to the latter. These suggested that FLF be likely derived continuously from lignin and other residue of terrestrial plant origin along the river network, and TYLF and TRLF be originated discontinuously from domestic wastewater in RUR-TOW. By high-throughput sequenced OTUs, the number of organisms in RUR-TOW could be higher than those in URB-IND, while genes associated with carbohydrate metabolism were lower in former than those in the latter. According to co-occurrence networks, microbes could promote the production of TYLF and TRLF in RUR-TOW. In contrast, microbial communities in URB-IND might contribute to decompose FLF. The obtained results could not only reveal interactions between DOM fractions and bacterial communities in the river network, but this methodology may be applied to other water bodies from different landscapes.

A novel recyclable hemoperfusion adsorbent based on TiO2 nanotube arrays for the selective removal of ß2-microglobulin.

Prolonged and excessive accumulation of ß2-microglobulin (ß2m) in the blood can lead to various kidney-related and other diseases. Currently, the most effective method of removing ß2m from the blood is hemoperfusion. Although some traditional hemoperfusion adsorbents such as cellulose and polystyrene microspheres have been used for the removal of ß2m, their selectivity still needs improvement. Immunosorbents have been developed to address this issue, but high cost and limited application are concerns. TiO2 nanotube arrays (TNTAs) have shown great potential in adsorption-related biomedical applications. In this study, we designed and developed a novel TNTA-based hemoperfusion adsorbent for the removal of ß2m, which has demonstrated good biocompatibility, selectivity, and reusability. We investigated the ß2m adsorption capacities of TNTAs with different pore sizes. The results indicate that TNTAs with a pore size matching the size of ß2m exhibit higher adsorption capacity while also having lower adsorption capacity for albumin, showing the importance of pore size on the selectivity of adsorbents. Additionally, green regeneration of TNTAs is achieved via the photocatalytic activity originating from TiO2. Even after five cycles, the adsorption capacity of TNTAs remained above 70%. Our work demonstrates that inorganic materials with ordered pores are capable to be candidates for hemoperfusion, possessing advantages over traditional organic materials such as high stability, security, and low cost.

Driving mechanism of water replenishment on DOM composition and eutrophic status changes of lake in arid and semi-arid regions of loess area.

Water replenishment can be a key factor in driving lake eutrophication status. In arid and semi-arid regions of China, water replenishment for a lake has been widely carried out for not only improving water environmental quality, but also maintaining ecological system function. However, it is still unclear in terms of mechanism by which water replenishment drives lake eutrophication status. In this study, fluorescence excitation-emission matrix spectroscopy (EEMs) combined with multiple statistical analysis models (including parallel factor analysis, correlation analysis, redundancy analysis, and partial least squares structural equation modeling) was utilized to reveal potential driving mechanism and causality between water replenishment, dissolved organic matter (DOM) fractions and eutrophic status of Lake Shahu in China. Based on variations of DOM fractions, fulvic-like substances could be accumulated during the replenishment period, while nutrients carried along the replenishment might conduce to increase microbial activities during the non-replenishment period. This should be contributed to an alteration of prominent component from fulvic-like substances to tyrosine-like substances during the replenishment period to non-replenishment period. According to partial least squares structural equation modeling, two potential indirect paths were finally revealed, i.e., water replenishment derived the eutrophic status of Lake Shahu: water replenishment → microbial activity → algae → eutrophication, and water replenishment → microbial activity → eutrophication. This supposed that the water replenishment should indirectly drive the algae and eutrophication of the lake by promoting the transformation of DOM fractions. In addition, natural conditions could indirectly contribute to the eutrophication of the lake through impacting the algae growth. These findings should be conducive to trace the alteration of DOM fractions in lakes by water replenishment and in recognizing potential driving mechanisms of water replenishment on eutrophication of lakes by changing DOM fractions. This could provide basic theoretical support for policymakers to regulate and treat the eutrophication of lakes.

Insight into the microbial nitrogen cycle in riparian soils in an agricultural region.

Riparian zones are considered as an effective measure on preventing agricultural non-point source nitrogen (N) pollution. However, the mechanism underlying microbial N removal and the characteristics of N-cycle in riparian soils remain elusive. In this study, we systematically monitored the soil potential nitrification rate (PNR), denitrification potential (DP), as well as net N2O production rate, and further used metagenomic sequencing to elucidate the mechanism underlying microbial N removal. As a whole, the riparian soil had a very strong denitrification, with the DP 3.17 times higher than the PNR and 13.82 times higher than the net N2O production rate. This was closely related to the high soil NO3--N content. In different profiles, due to the influence of extensive agricultural activities, the soil DP, PNR, and net N2O production rate near the farmland edge were relatively low. In terms of N-cycling microbial community composition, the taxa of denitrification, dissimilatory nitrate reduction, and assimilatory nitrate reduction accounted for a large proportion, all related to NO3--N reduction. The N-cycling microbial community in waterside zone showed obvious differences to the landside zone. The abundances of N-fixation and anammox genes were significantly higher in the waterside zone, while the abundances of nitrification (amoA&B&C) and urease genes were significantly higher in the landside zone. Furthermore, the groundwater table was an important biogeochemical hotspot in the waterside zone, the abundance of N-cycle genes near the groundwater table was at a relative higher level. In addition, compared to different soil depths, greater variation in N-cycling microbial community composition was observed between different profiles. These results reveal some characteristics of the soil microbial N-cycle in the riparian zone in an agricultural region and are helpful for restoration and management of the riparian zone.

Insight into suppression of dibutyl phthalate on DOM removal during municipal sewage treatment using fluorescence spectroscopy with PARAFAC and moving-window 2D-COS.

Dibutyl phthalate (DBP) has been widely detected in municipal and industrial wastewater, which could indirectly inhibit pollutant removals, especially degradation of dissolved organic matter (DOM). Here, the inhibition of DBP on DOM removal from wastewater in pilot-scale A2O-MBR system was investigated by fluorescence spectroscopy with two-dimensional correlation (2D-COS) and structural equation modeling (SEM). Seven components were extracted from DOM using parallel factor analysis, i.e., tryptophan-like (C1 and C2), fulvic-like (C4), tyrosine-like (C5), microbial humic-like (C6) and heme-like (C7). The tryptophan-like had a blue-shift at DBP occurrence, defined as blue-shift tryptophan-like (C3). DBP with 8 mg L-1 exhibited a stronger inhibition on removals of DOM fractions, extraordinarily tyrosine-like and tryptophan-like in anoxic unit than DBP of 6 mg L-1 by moving-window 2D-COS. The indirect removals of C1 and C2 through the C3 removal were more strongly inhibited by 8 mg L-1 DBP than those by 6 mg L-1 DBP, while the former exhibited a weaker inhibition on the direct degradation of C1 and C2 than the latter via SEM. Based on metabolic pathways, abundances of key enzymes secreted by microorganism in anoxic unit, degrading tyrosine-like and tryptophan-like, were higher in wastewater with 6 mg L-1 DBP than those with 8 mg L-1 DBP. These could provide a potential approach for online monitoring of DBP concentrations in wastewater treatment plants, which could rectify operating parameters, and then enhance the treatment efficiencies.

Explore variations of DOM components in different landcover areas of riparian zone by EEM-PARAFAC and partial least squares structural equation model.

Dissolved organic matter (DOM) plays key roles in species-distribution of contaminants and the biogeochemical cycle of carbon in ecosystems. Riparian zone is the representative of water-land ecotone and controls the DOM exchange between water and land. However, the variance of DOM in different landcover areas of an urban river riparian zone is unknown. In this study, fluorescence excitation-emission matrix (EEM) spectroscopy coupled with parallel factor analysis (PARAFAC) and partial least squares structural equation model (PLS-SEM) was applied to character dissolved organic matter (DOM) fractions in four types of landcover riparian areas (natural forest, artificial forest, semi-natural grassland, and cropland) of Puhe River and trace latent factors. Soil samples were collected at 0-20 cm, 20-40 cm, 40-60 cm, and 60-80 cm. The results showed that soil DOM components and humification varied between forests with grassland and cropland samples, and soil humification was obviously higher in the forest samples than that in the grassland and cropland samples. In the natural and artificial forest soils, the humic/fulvic-like were the dominant fractions of DOM, whose variations were smaller than the protein-like with soil depths. However, the tyrosine-like was the representative component in the grassland and cropland soils, whose variation was smaller than the humus substances. According to the PLS-SEM, the DOM components and humification were affected by soil physiochemical properties and DOM sources. The humification in the forest soils had a positive correlation with tryptophan-like, which derived from blended source of the autochthonous and terrigenous. Nevertheless, a positive correlation was observed between humification and humus substances, which could derive from microbial degradation of tyrosine-like, in the grassland and cropland soils. Moreover, the soil physiochemical properties were negatively related to DOM components in all soil samples, which could affect indirectly soil humification. Therefore, EEM combined with PARAFAC and PLS-SEM might be an effective method to investigate DOM fractions and trace the latent factors in different landcover areas of the riparian zone.

Impacts of dibutyl phthalate on bacterial community composition and carbon and nitrogen metabolic pathways in a municipal wastewater treatment system.

Dibutyl phthalate (DBP) is a typical toxic and hazardous pollutant in pharmaceutical wastewater, affecting the metabolism of microbial flora, leading to decreased treatment efficiency, and deteriorated effluent quality in municipal wastewater treatment plants (WWTPs). This study conducted a long-term experiment with 6 operational stages in a pilot-scale A2O-MBR system, analyzing the effect of DBP on the bacterial community and their carbon and nitrogen metabolic pathways. 16S rRNA gene amplicon sequencing analysis and principal components analysis (PCA) showed that DBP at 8 mg/L significantly influenced the structure of bacterial community (P < 0.05), resulting in reduced bacterial community diversity. Metagenomic analysis was used to explore the embedded carbon and nitrogen metabolic pathways. At the presence of DBP, the metabolism of saccharides, lipids, and aromatic compounds were blocked owing to the vanishment of key enzyme (such as acetylaminohexosyltransferase (EC 2.4.1.92) and UDP-sugar pyro phosphorylase (EC 2.7.7.64)) encoding genes, resulting in weakened carbon metabolism, and thus reduced COD removal performance. The resultant deficiency of the genes such as those encoding hydroxyproline dehydrogenase (EC 1.5.5.3) gave rise to interrupted metabolic pathways of amino acid (arginine, proline, tyrosine, and tryptophan), resulting in declined function of nitrogen metabolism and thus reduced TN removal efficiency. The uncovery of the mechanisms by which DBP affects wastewater treatment system efficiency and microbial metabolism is of theoretical importance for the efficient operation of municipal and pharmaceutical wastewater treatment systems.

Deletion of African Swine Fever Virus (ASFV) H240R Gene Attenuates the Virulence of ASFV by Enhancing NLRP3-Mediated Inflammatory Responses.

African swine fever (ASF) is a highly contagious infectious disease of domestic pigs and wild boars caused by African swine fever virus (ASFV), with a mortality rate of up to 100%. In order to replicate efficiently in macrophages and monocytes, ASFV has evolved multiple strategies to evade host antiviral responses. However, the underlying molecular mechanisms by which ASFV-encoded proteins execute immune evasion are not fully understood. In this study, we found that ASFV pH240R strongly inhibits transcription, maturation, and secretion of interleukin-1ß (IL-1ß). Importantly, pH240R not only targeted NF-κB signaling but also impaired NLRP3 inflammasome activation. In this mechanism, pH240R interacted with NF-kappa-B essential modulator (NEMO), a component of inhibitor of kappa B kinase (IKK) complex and subsequently reduced phosphorylation of IκBα and p65. In addition, pH240R bonded to NLRP3 to inhibit NLRP3 inflammasome activation, resulting in reduced IL-1ß production. As expected, infection with H240R-deficient ASFV (ASFV-ΔH240R) induced more inflammatory cytokine expression both in vitro and in vivo than its parental ASFV HLJ/18 strain. Consistently, H240R deficiency reduced the viral pathogenicity in pigs compared with its parental strain. These findings reveal that the H240R gene is an essential virulence factor, and deletion of the H240R gene affects the pathogenicity of ASFV HLJ/18 by enhancing antiviral inflammatory responses, which provides insights for ASFV immune evasion mechanisms and development of attenuated live vaccines and drugs for prevention and control of ASF. IMPORTANCE African swine fever (ASF), caused by African swine fever virus (ASFV), is a highly contagious and acute hemorrhagic viral disease of domestic pigs, with a high mortality approaching 100%. ASFV has spread rapidly worldwide and caused huge economic losses and ecological consequences. However, the pathogenesis and immune evasion mechanisms of ASFV are not fully understood, which limits the development of safe and effective ASF attenuated live vaccines. Therefore, investigations are urgently needed to identify virulence factors that are responsible for escaping the host antiviral innate immune responses and provide a new target for development of ASFV live-attenuated vaccine. In this study, we determined that the H240R gene is an essential virulence factor, and its depletion affects the pathogenicity of ASFV by enhancing NLRP3-mediated inflammatory responses, which provides theoretical support for the development of an ASFV attenuated live vaccine.

Molecular Mechanisms of Poxvirus Evolution.

Poxviruses are often thought to evolve relatively slowly because they are double-stranded DNA pathogens with proofreading polymerases. However, poxviruses have highly adaptable genomes and can undergo relatively rapid genotypic and phenotypic change, as illustrated by the recent increase in human-to-human transmission of monkeypox virus. Advances in deep sequencing technologies have demonstrated standing nucleotide variation in poxvirus populations, which has been underappreciated. There is also an emerging understanding of the role genomic architectural changes play in shaping poxvirus evolution. These mechanisms include homologous and nonhomologous recombination, gene duplications, gene loss, and the acquisition of new genes through horizontal gene transfer. In this review, we discuss these evolutionary mechanisms and their potential roles for adaption to novel host species and modulating virulence.

Applying synchronous fluorescence spectra with Gaussian band fitting and two-dimensional correlation to characterize structural composition of DOM from soils in an aquatic-terrestrial ecotone.

Dissolved organic matter (DOM), the primary participant of carbon and nitrogen cycle, has a great impact on the behavior and fate of organic pollutants and heavy metals in eutrophic lakes. The dynamic spectral properties of DOM fractions were revealed in an aquatic-terrestrial ecotone under the different types of land use. Composite soil samples of different depths (0-20, 20-40 and 40-60 cm) were collected from four different land uses along a disturbed-impact gradient in Taihu Lake, China, i.e., grassland (GRL), forest land (FOL), paddy field (PAF), and vegetable field (VEF). DOM mainly consisted of tyrosine-like material (TYLF), tryptophan-like material (TRLF), microbial humic-like material (MHLF), fulvic-like material (FLF) and humic-like material (HLF) within all soil profiles, where TRLF was the dominant component (61.30 %) using synchronous fluorescence spectroscopy (SFS) combined with principal component analysis and Gaussian band fitting. Based on two-dimensional correlation spectroscopy with SFS and Fourier transform infrared, the variation order of DOM fractions was FLF → MHLF → HLF → TRLF → TYLF within the GRL soil profile, and MHLF exhibited an oppositive change with aliphatic OH and amide I in protein. The order of DOM fractions was MHLF → FLF → HLF → TYLF → TRLF within the FOL soil profile, and the change trend of MHLF remained oppositive with aliphatic OH and CO in ester. The order of DOM within the PAF soil profile fractions was TRLF → MHLF → HLF → TYLF → FLF, and changing trends of TYLF were oppositive to aliphatic OH, CH bending vibration, CH bending vibration and CO in ester. The order of DOM fractions was HLF → TYLF → TRLF → FLF → MHLF within the VEF soil profile, where the changing trend TYLF remained oppositive to aliphatic OH, CH deformations in lignin and aliphatic group and amide I in protein. This study may provide important support for alleviating lake water eutrophication or pollution.

Applying EEM-PARAFAC combined with moving-window 2DCOS and structural equation modeling to characterize binding properties of Cu (II) with DOM from different sources in an urbanized river.

Dissolved organic matter (DOM) in aquatic environment distinctly affects the behavior and fate of heavy metals via complexation, while the interfacial mechanisms and processes are still lacking in detail. Here, Cu (II) binding characteristics of DOM originated from hilly (NDOM), rural (RDOM) and urban (UDOM) regions in an urbanized river was explored by fluorescence excitation-emission matrix spectroscopy (EEM) combined with principal component coefficients, parallel factor analyses (PARAFAC), moving-window two-dimensional correlation spectroscopy (MW2DCOS) and structural equation modeling (SEM). Eight components were extracted from the titrants through EEM-PARAFAC, i.e., phenol-like substance (C1), tyrosine-like substance (C2), visible tryptophan-like substance (C3), ultraviolet tryptophan-like substance (C4), recent biological production (C5), wastewater-derived organic matter (C6), microbial humic-like substance (C7) and fulvic-like substance (C8). Interestingly, NDOM only contained C1, C3, C5 and C8, while nearly all components were found in RDOM (except for C2) and UDOM (except for C4). The f value of C1 (1.239) in NDOM was much higher than those in RDOM (0.134) and UDOM (0.115), so was of C8. It indicated that phenol-like and fulvic-like derived from autochthonous sources exhibited great binding ratios in the complexation with Cu (II). Moreover, C3 and C5 from UDOM exhibited higher f values (0.591 and 1.983) than those from NDOM and RDOM, suggesting that Cu (II) has a great binding capacity on protein-like from domestic and industrial wastewater. The MW2DCOS revealed that phenol-like and protein-like in NDOM and RDOM were essential for the binding of 160 µmol L-1 Cu (II), whereas fulvic-like in NDOM and UDOM could react significantly with 10 µmol L-1 Cu (II). Based on SEM, Cu (II) concentration had a negative direct effect on the fluorescence intensity of C7 or C8, whereas it showed an indirect positive effect on C7 or C8 through influencing C5, so was C6. It suggested that Cu (II) showed an indirect positive effect on the C8. This study might present a further comprehend of the environmental behaviors of Cu (II) in rivers.

Impacts of dibutyl phthalate on biological municipal wastewater treatment in a pilot-scale A2/O-MBR system.

Dibutyl phthalate (DBP) is a typical contaminant in pharmaceutical wastewater with strong bio-depressive properties which potentially affects the operation of municipal wastewater treatment systems. Based on a year-round monitoring of the quality of influent and effluent of a full-scale pharmaceutical wastewater treatment plant in Northeast China, the DBP was found to be the representative pollutant and its concentration in the effluent ranged 4.28 ± 0.93 mg/L. In this study, the negative effects of DBP on a pilot-scale A2/O-MBR system was investigated. When the influent DBP concentration reached 8.0 mg/L, the removals of chemical oxygen demand (COD) and total nitrogen (TN) were significantly inhabited (P < 0.01), with the effluent concentration of 54.7 ± 2.6 mg/L and 22.8 ± 3.7 mg/L, respectively. The analysis of pollutant removal characteristics of each process unit showed that DBP had the most significant effects on the removals of COD and TN in the anoxic tank. The α- and ß-diversity in the system decreased significantly when the influent DBP concentration reached 8.0 mg/L. The impacts of DBP on known nitrifying bacteria, such as Nitrospira, and phosphorus accumulating organisms (PAOs), such as Cadidatus Accumulibacter, were not remarkable. Whereas, DBP negatively affected the proliferation of key denitrifying bacteria, represented by Simplicispira, Dechloromonas and Acinetobacter. This study systematically revealed the impacts of DBP on the pollutants removal performance and the bacterial community structure of the biological municipal wastewater treatment process, which would provide insights for understanding the potential impacts of residues in treated pharmaceutical wastewater on biological municipal wastewater treatment.

African swine fever virus pE301R negatively regulates cGAS-STING signaling pathway by inhibiting the nuclear translocation of IRF3.

African swine fever (ASF) is a highly contagious and lethal infectious disease of domestic pigs and wild boars by the African swine fever virus (ASFV). ASFV infects domestic pigs with the mortality rate approaching 100 % at acute stage of infection. The cGAS-STING-mediated antiviral responses are wildly accepted that cGAS acts as DNA sensor for sensing of viral DNA during DNA virus infection. However, the molecular mechanisms underlying negatively regulation of cGAS-STING signaling and type I IFN (IFN-I) production by ASFV proteins are not fully understood. In this study, we demonstrated that ASFV pE301R antagonize the activities of IFN-ß-, NF-κB-, ISRE-luciferase (Luc) reporters-induced by cGAS-STING in a dose dependent manner. Consistent with these results, the mRNA levels of Ifnb1, Isg15, Isg56 are attenuated by ASFV pE301R. Furthermore, ASFV pE301R executes its inhibitory function at the downstream of IFN-regulatory factor 3 (IRF3) phosphorylation. Mechanistically, pE301R interacts with IRF3 via its amino acid (aa) 1-200 region, resulting in inhibition of the nuclear translocation of IRF3 induced by cGAMP and poly(dA:dT). Overall, our findings reveal that pE301R acts as a negatively regulator to inhibit IFN-I production and to subvert host antiviral innate immunity during ASFV infection.

Maladaptation after a virus host switch leads to increased activation of the pro-inflammatory NF-κB pathway.

Myxoma virus (MYXV) causes localized cutaneous fibromas in its natural hosts, tapeti and brush rabbits; however, in the European rabbit, MYXV causes the lethal disease myxomatosis. Currently, the molecular mechanisms underlying this increased virulence after cross-species transmission are poorly understood. In this study, we investigated the interaction between MYXV M156 and the host protein kinase R (PKR) to determine their crosstalk with the proinflammatory nuclear factor kappa B (NF-κB) pathway. Our results demonstrated that MYXV M156 inhibits brush rabbit PKR (bPKR) more strongly than European rabbit PKR (ePKR). This moderate ePKR inhibition could be improved by hyperactive M156 mutants. We hypothesized that the moderate inhibition of ePKR by M156 might incompletely suppress the signal transduction pathways modulated by PKR, such as the NF-κB pathway. Therefore, we analyzed NF-κB pathway activation with a luciferase-based promoter assay. The moderate inhibition of ePKR resulted in significantly higher NF-κB­dependent reporter activity than complete inhibition of bPKR. We also found a stronger induction of the NF-κB target genes TNFα and IL-6 in ePKR-expressing cells than in bPKR-expressing cells in response to M156 in both transfection and infections assays. Furthermore, a hyperactive M156 mutant did not cause ePKR-dependent NF-κB activation. These observations indicate that M156 is maladapted for ePKR inhibition, only incompletely blocking translation in these hosts, resulting in preferential depletion of short­half-life proteins, such as the NF-κB inhibitor IκBα. We speculate that this functional activation of NF-κB induced by the intermediate inhibition of ePKR by M156 may contribute to the increased virulence of MYXV in European rabbits.