ABSTRACT
PURPOSE: The aim of this study was to investigate the effects of intra-articular resveratrol injections on cartilage destruction and synovial inflammation in an experimental temporomandibular joint osteoarthritis (TMJ-OA) model. MATERIALS AND METHODS: Freund's complete adjuvant injection method was used to construct the TMJ-OA model. Twenty-eight male Wistar rats were randomly placed into 4 groups: control (n = 4), TMJ arthritis (n = 8), low-dose intra-articular resveratrol (RES[L]; n = 8), and high-dose intra-articular resveratrol (RES[H]; n = 8). Intra-articular injections of resveratrol were performed 3 times at 1-week intervals, 1 week after the administration of a single dose of Freund's complete adjuvant to the TMJ. The effects of resveratrol on cartilage destruction and synovial inflammation were examined histopathologically. The histomorphometric examination revealed condylar cartilage and articular disc thickness. An apoptotic chondrocyte count was performed with terminal deoxynucleotidyl transferase dUTP nick end labeling staining, and matrix metalloproteinase 13 expression was evaluated through an immunohistochemical examination. RESULTS: The thickness of the condylar cartilage in the RES(L) and RES(H) groups was statistically significantly greater than that in the control and TMJ arthritis groups (P < .05). The inflammation-induced articular disc thickening was significantly lower in the RES(L) and RES(H) groups (P < .05). The chondrocyte apoptosis in the RES(L) and RES(H) groups was significantly lower than that in the TMJ arthritis group (P < .05). The matrix metalloproteinase 13 expression in the RES(L) and RES(H) groups was obviously less than that in the TMJ arthritis group (P < .05). CONCLUSIONS: The intra-articular resveratrol treatment exerted a curative effect by preventing the inflammation and cartilage destruction associated with TMJ-OA.
Subject(s)
Cartilage, Articular , Osteoarthritis , Animals , Inflammation/drug therapy , Injections, Intra-Articular , Male , Osteoarthritis/drug therapy , Rats , Rats, Wistar , Resveratrol , Temporomandibular JointABSTRACT
OBJECTIVES: The aim of this study was to evaluate the importance of neopterin levels in brucellosis and its alterations with treatment. METHODS: Thirty patients who had positive clinical findings, Brucella standard tube agglutination tests and/or positive blood culture were included in the study. The control group was composed of 30 healthy subjects. Neopterin (NPT) levels were measured by ELISA according to the protocol of manufacturer. RESULTS: Mean NPT levels were 54.15+/-33.7 nmol/l in study groups and 8.75+/-2.48 nmol/l in controls (P<0.001). It was determined that NPT levels measured at the time of admission of the patients had a linear correlation between the duration of complaint (r=0.560, P=0.001). While the mean NPT levels in patients with complaints of over 30 days were 79.07+/-34.9 nmol/l and it was 39.71+/-23.4 nmol/l in the patients with complaints of less than or equal to 30 days (P=0.002). NPT values measured after treatment (20.48+/-25.6) was determined lower by statistical means than the values measured before treatment (54.15+/-33.7, P=0.0001). The NPT levels of patients with positive blood culture (75.56+/-33.6) were higher than those of with negative blood cultures (32.72+/-15.2, P=0.001). CONCLUSION: It was concluded that the NPT levels were higher in patients with brucellosis. In addition, it was found that the levels of NPT were higher in non-responding patients than responded to the brucellosis treatment. It was thought that monitoring NPT levels could be useful for the extent and activity of the disease.
Subject(s)
Brucella melitensis , Brucellosis/diagnosis , Neopterin/blood , Agglutination Tests/methods , Blood Sedimentation , Brucella melitensis/isolation & purification , Brucellosis/blood , Brucellosis/drug therapy , C-Reactive Protein/analysis , Case-Control Studies , Enzyme-Linked Immunosorbent Assay/methods , Female , Humans , Leukocyte Count/methods , Male , Prognosis , Treatment OutcomeABSTRACT
Brucellosis is a zoonosis that affects several organs and has a protean presentation. The authors report the case of a 61-year-old male patient with brucellar spondylodiscitis involving several vertebrae and a paravertebral abscess localized in the erector spinae muscle. Diagnosis was made by positive blood culture and MRI. No relapse was seen with a combined treatment (doxycycline/rifampin) for 3 months, followed by doxycycline alone for 6 months. Almost all radiologic findings disappeared at the end of a 1-year follow-up without any further treatment.
Subject(s)
Brucellosis/complications , Discitis/etiology , Lumbar Vertebrae/pathology , Thoracic Vertebrae/pathology , Abscess/drug therapy , Abscess/microbiology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Brucella/isolation & purification , Brucellosis/drug therapy , Discitis/drug therapy , Discitis/microbiology , Doxycycline/administration & dosage , Doxycycline/therapeutic use , Drug Therapy, Combination , Fever/etiology , Fever/microbiology , Humans , Low Back Pain/drug therapy , Low Back Pain/etiology , Magnetic Resonance Imaging , Male , Middle Aged , Rifampin/administration & dosage , Rifampin/therapeutic use , Treatment OutcomeABSTRACT
In this prospective study, we investigated the serum levels of hepatocyte growth factor (HGF) and C-reactive protein (CRP) before and after the treatment of patients with acute brucellosis. The study comprised 58 patients with acute brucellosis and 30 healthy volunteers. Pre-treatment serum HGF levels of 58 patients with acute brucellosis (1548.6 +/- 220.1) were significantly higher than levels of the control group (401.4 +/- 69.7) (p < 0.001). Serum levels of HGF and CRP significantly decreased at the end of the treatment period (p < 0.001). Post treatment, levels did not differ from those of the control group (p > 0.05). Serum HGF levels of patients with acute brucellosis correlated to CRP and ALT levels (r: 0.922, 0.752; p < 0.001, respectively). Our findings suggest that serum HGF levels may be used as a supplementary marker to evaluate the effectiveness of the treatment in patients with acute brucellosis.
