Factors influencing thrombelastography in pregnancy / 中南大学学报(医学版)

OBJECTIVES@#The number of gestational women has been increased in recent years, resulting in more adverse pregnancy outcomes. It is crucial to assess the coagulation function of pregnant women and to intervene in a timely manner. This study aims to analyze the influencing factors on thrombelastography (TEG) and explore the evaluation of TEG for gestational women.@*METHODS@#A retrospective study was conducted on 449 pregnant women who were hospitalized in the obstetrics department in Xiangya Hospital of Central South University from 2018 to 2020. We compared the changes on the TEG parameters among normal pregnant women between different age groups, different ingravidation groups, and different stages of pregnancy groups. The influence on TEG of hypertensive disorders in pregnancy (HDP) and gestational diabetes mellitus (GDM) as well as two diseases synchronization was explored.@*RESULTS@#Compared with the normal second trimester women, the R values and K values of TEG were increased, and α angle, CI values and LY30 values were decreased in third trimester women (all P<0.05). Compared with normal group, the R values and CI values of TEG of the HDP group have significant difference (both P<0.05). There were no significant difference of TEG between the GDM group, the HDP combined with GDM group and the normal group (all P>0.05). Multiple linear regression analysis showed that the influencing factors for R value in TEG were weeks of gestation (P<0.001) and mode of conception (P<0.05), for α angle was weeks of gestation (P<0.05), for MA value was mode of conception (P<0.05), and for CI value was weeks of gestation (P<0.05). The analysis of correlation between TEG with platelet (PLT) and coagulation routines represented that there was a correlation between TEG R values and activated partial thromboplastin time (APTT) (P<0.01), and negative correlation between TEG CI values and APTT (P<0.05). There was a negative correlation between TEG K values and FIB (P<0.05). The correlation of α angle (P<0.05), MA values (P<0.01) and CI values (P<0.05) with FIB were positive respectively.@*CONCLUSIONS@#The TEG parameters of 3 stages of pregnancy were different. The different ingravidation approach has effect on TEG. The TEG parameters were consistent with conventional coagulation indicators. The TEG can be used to screen the coagulation status of gestational women, recognize the abnormalities of coagulation and prevent the severe complication timely.

Effects of combined spinal epidural anesthesia and general anesthesia in cesarean section for pediatric ;respiration / 中国基层医药

Objective To compare the effect of combined spinal epidural anesthesia and general anesthesia for cesarean section surgery on pediatric respiration,discuss the choice of anesthesia in caesarean operation.Methods 140 cases of maternal line cesarean section were randomly divided into control group and observation group,70 cases in each group.Control group used propofol,sufentanil and cis atracurium intravenous induction,after fetal take out used conventional medicine.The observation group used combined spinal epidural anesthesia with ropivacaine.Recor-ded the operation time,cut skin to fetal childbirth time and uterine incision to fetal childbirth time.Fetal umbilical arterial blood for blood gas analysis,compared the two groups of Apger score at 1min,5min,10min after birth,the first breath of time,and the use of auxiliary breathing,chest compressions and total drug recovery.Results The operation time,cut skin to take time out and uterine incision to fetus fetus in the two groups had no statistical differences(t =0.974,P =0.332;t =0.067,P =0.947;t =0.803,P =0.423).Apger score at 1min in the observation group [(8.91 ±0.75)min]was better than the control group[(9.26 ±0.86)min](t =-2.566,P =0.011),while Apger score between the two groups at 5min,10min had no statistically significant differences(t =-0.533,P =0.595;t =-0.299,P =0.765).The pediatric first breath[(7.17 ±3.26)s]of the observation group was shorter than the control group[(21.34 ±5.75)s](t =17.936,P <0.001).pH,PaCO2 ,PaO2 in umbilical artery blood of the two groups had no statistically significant differences(t =-0.438,P =0.662;t =0.102,P =0.919;t =-0.524,P =0.601).In the observation group used auxiliary breathing,chest compressions and drug recovery in all 7 cases,which was less than 15 cases in the control group,but there was no statistically significant difference (χ2 =3.451,P =0.063).Conclusion The effect of combined spinal epidural anesthesia for cesarean section in pediatric breathing is smaller,it can be used in a cesarean section safely,and worth popularizing in clinical application.

Hybridization monitor: a method for identifying differences between complex genomes.

We have developed a method to identify and amplify differential fragments between two complex genomes. This technique, named hybridization-monitored genome differential analysis (HMDA), incorporates a monitor system into a PCR-based solid subtraction hybridization that tracks the entire hybridization process. This is achieved by monitoring the subtraction progress using PCR analysis of the conserved sequence of 18S rDNA in the tester sample after each round of subtraction. Homologous fragments can then be eliminated when bound to the driver DNA immobilized on a solid membrane. The hybridization continues until the conserved DNA sequence of 18S rDNA can no longer be detected, and most of the unbound DNA fragments left in the liquid were mainly the tester-specific fragments, thus greatly decreasing the complexity of DNA template of PCR amplification, increasing the amplification efficiency of differences accordingly, and ensuring high positive efficiency and coverage across the tester genome. We have applied the technique in a comparison between the genomes of Saccharomyces cerevisiae and Schizosaccharomyces pombe, which are two completely sequenced organisms. Results indicated that 95% of the subtracted clones have been confirmed to be different to the driver analyzed using the BLASTN homology alignment. With this technique, 240-fold enrichment of differences is obtained, and the coverage of the difference is up to 79%. These results indicate that HMDA can efficiently identify sequences that differ between two complex genomes.

ISOLATION AND IDENTIFICATION OF THE CELLULOSE-UTILIZINGBACTERIA FROM THE GUT OF APRIONA GERMATI(HOPE) / 微生物学通报

In order to clarify the cellulose-utilizing mechanism of the Cerambycidae, the microorganism in the Apriona germari(Hope) larvae's gut was cultured and identified. A strain of facultative anaerobic cellulose-utilizing bacteria was isolated from the midgut fluid of A. germari larvae with the cellulose-congo red agar medium. The colonies of the bacteria were white and round with regular margin. Clear zones of cellulolysis surrounding the colonies might reach 10m～20mm. The size of the bacteria was 0.5μm～0. 8μm× l～3μm approximately. The cell was gram-positive rod with polar flagella. It hadn't spores. With the results of biochemical tests, the bacteria were tentatively identified as Cellulomonas.

ISOLATION AND IDENTIFICATION OF THE CELLULOSE-UTILIZING BACTERIA FROM THE GUT OF APRIONA GERMATI(HOPE) / 微生物学通报

In order to clarify the cellulose utilizing mechanism of the Cerambycidae, the microorganism in the Apriona germari(Hope) larvae’s gut was cultured and identified. A strain of facultative anaerobic cellulose utilizing bacteria was isolated from the midgut fluid of A. germari larvae with the cellulose congo red agar medium. The colonies of the bacteria were white and round with regular margin. Clear zones of cellulolysis surrounding the colonies might reach 10mm～20mm. The size of the bacteria was 0.5?m～0.8?m?1～3?m approximately. The cell was gram positive rod with polar flagella. It hadn't spores. With the results of biochemical tests, the bacteria were tentatively identified as Cellulomonas.