ABSTRACT
Albopeptins produced by Streptomyces albofaciens JC-82-120 were isolated as effective antibiotics for plant pathogenetic disease in 1986. However, their unusual physicochemical properties hampered the determination of their chemical structures. In this report, we describe our efforts to elucidate their structures. Initially, the structure of an unusual C13-fatty acid with an N-hydroxyguanidyl group was determined using degradation and chemical synthesis. After the linear portion of the octapeptide core was constructed based on the 2D-NMR data, the final assembly of the unusual structure, including the sulfoxide bridge, was achieved through the analysis of detailed NMR data. The proposed structure of albopeptin B was supported by MS/MS data, which also enabled us to determine the structure of 5 albopeptin family members. Bioinformatics analysis of the genomic data of the producer strain further led us to propose that their biosynthetic pathway is similar to the ribosomally derived lanthipeptides possessing a long-chain fatty acid.
Subject(s)
Anti-Bacterial Agents , Lipopeptides , Anti-Bacterial Agents/chemistry , Biosynthetic Pathways/genetics , Fatty Acids , Multigene Family , Tandem Mass SpectrometryABSTRACT
Objective To explore the advantage of Body-Jet hydrodynamic liposuction system in autologous fat transplantation.Methods Through the unique hydrodynamic jet washing liposuction technology and the closed fat storage system,the fat was filtrated and purified,and then the fat was injected into the posterior space of greater pectoral muscle and breast tissue as well as subcutanous tissue of the breast with unique reverse-directed double orifice injector.During May 2015 to July 2016,27 patients were treated in our hospital and followed up for 30 to 180 days.Results All patients had good incision healing;there were no rugged skin,hematoma,seroma and other complications in the liposuction site.In 25 patients after augmentation the breast had rounded appearance and good feeling;1 patient presented liquefied fat,1 patient had poor survival of the injected fat;later 2 cases underwent second surgery.Conclusions The liposuction system can be applied in autologous adipose transplantation breast augmentation.The procedure is simple and quick.The survival rate after adipose transplantation is high with quick recovery.The shape of the breast is full and round with good feeling.
ABSTRACT
Objective To investigate the reason and prevention of complications of head and neck expander implant.Methods From May,2003 to October,2007,patients with expander implant in the skin soft tissues of head and neck were retrospectively reviewed.Results A total of 139 expanders were implanted in 68 patients.Complications caused by the expander included appearance(1 case),leaking(1 case),haematoma(2 cases)and infection(2 cases).Conclusion The complications induced by expander implanted in head and neck are more commonly occurred.The prevention and early detection of the complication are important to decrease its ineidence and the degree of damage.Moreover,surgeon's procedure and operational methods are main factors in the prevention of complications.
