ABSTRACT
The side effects of kwao keur dietary supplements (obtained from the tuberous root of Pueraria mirifica) have recently been reported by the Ministry of Health, Labour and Welfare, Japan. To control the quality of kwao keur products, its ingredients need to be maintained by characteristic marker compounds, such as miroestrol, deoxymiroestrol, and kwakhurin (KWA). In this study, we described the facile synthesis of KWA, a marker compound of P. mirifica. Our revised synthetic method produced KWA with shorter steps and higher yield than the reported method. Furthermore, the absolute purity of KWA was determined by quantitative NMR analysis for standardization as a reagent, and its purity was 92.62 ± 0.12%.
Subject(s)
Isoflavones/chemical synthesis , Magnetic Resonance Spectroscopy/standards , Pueraria/chemistry , Dietary Supplements/standards , Drug Design , Isoflavones/chemistry , Isoflavones/standards , Plant Roots/chemistry , Plant Roots/metabolism , Pueraria/metabolism , Reference StandardsABSTRACT
Under the irradiation of red light (690 nm), quinones were converted to hydroquinones by thiols in the presence of metallophthalocyanines. The reaction proceeded via the charge separation between the triplet state of phthalocyanine and the quinone. The product determining step was protonation of the quinone anion radical, as indicated by the fact that the reaction was accelerated by the use of more acidic thiols or addition of an acid.
