Wild garlic extract reduces lipid peroxidation in terbuthylazine-treated human erythrocytes.

Background: Among other negative effects, herbicides induce oxidative stress, leading to lipid peroxidation and protein oxidation. Therefore, there is a growing need to identify natural compounds with sufficient antioxidant capacity and mitigate the negative effects of herbicides without side effects.Objective: Our study aimed to examine the protective effect of the phenolic extract of wild garlic (WG) leaves on terbuthylazine-treated erythrocytes.Material and methods: In human erythrocytes treated with the herbicide terbuthylazine (4.5 mg/L) alone and a combination of terbuthylazine and WG extract, we measured malondialdehyde (MDA) and haemoglobin (Hb) concentrations and the antioxidant activities of CuZn superoxide dismutase (SOD1; EC 1.15.1.1) and catalase (CAT; EC 1.11.1.6) in vitro.Results: In comparison with terbuthylazine, WG extract reduced the concentrations of MDA and Hb from 59.69 to 43.45 nmol/gHb (27%, p < 0.001) and 165.08 to 128.64 g/L (22%, p < 0.05), respectively. Catalase activity was induced for samples treated with both WG extract and terbuthylazine compared with terbuthylazine alone (p < 0.05).Conclusions: The results demonstrated that WG may reduce the toxicity of terbuthylazine, and the erythrocyte membrane may be the primary site of phenolic action. Therefore, the lipid peroxidation intensity could be a biomarker of oxidative damage caused by terbuthylazine and the protective effect of WG.

Changes in the physicochemical, antioxidant and antibacterial properties of honeydew honey subjected to heat and ultrasound pretreatments.

The objective of this study was to examine the effect of different treatments on the physicochemical, antioxidant, and antibacterial properties of honeydew honey. Honeydew honey was subjected to heat treatment and 9 different ultrasound treatments. Our results showed that the following parameters were significantly changed: water content, pH, electrical conductivity, diastase activity, HMF content and water activity. The ultrasound resulted in an increase in the total phenol content and the antioxidant capacity (DPPH, FRAP, and ABTS tests) in comparison with the conventional thermal technique. In most cases, the samples subjected to ultrasound improved the antibacterial activity; the heat treatment resulted in a significant reduction of the antibacterial activity, and sample 4 (ultrasound 30 °C, 5 min) showed the best antibacterial activity. The ultrasound treatment, especially at lower temperatures, represents a technique that enables the preservation and improvement of the biological properties of honeydew honey.

Comparative study of toxic heavy metal residues and other properties of honey from different environmental production systems.

Honeybees forage a large spatial area around the hives. In addition, honey production takes place in various environments, and polluted environment is often hard to detect. It impacts both human and beehive health, especially through honey which is used for human consumption. Pollen analysis was conducted by a novel approach through a multivariate principal component analysis where it was possible to obtain grouping patterns related to foraging plant species. Samples of honey were acquired from three different environmental production systems: (i) honey from the apiaries in the vicinity of thermal power plant, (ii) apiary of certified organic production and (iii) the conventional production with semi-controlled production. Significantly higher contents of the Pb, Cd and Zn are found in the analysed honeys taken near the thermal power plant compared with those of the other analysed honeys. The origin of Zn, Pb and Cd in the honey is the contaminated forage plants and foraging honeybees. Honey from certified organic production differentiated significantly from other two types of production by the water content, electrical conductivity and total soluble solids and notably it contained significantly less ash and lead. There is a clear advantage of certified organic honey in terms of heavy metal residues as the most prominent pollution factor in honey. Therefore, honey can be used as the broad range environmental pollution indicator, as bees will forage on polluted plants and bring the pollutant from a wide spatial range inside the hive, where it can be traced in the honey. Graphical abstract.