ABSTRACT
Irritable bowel syndrome (IBS) is the most frequent functional gastrointestinal disorder, worldwide, with a high prevalence among Mestizo Latin Americans. Because several inflammatory disorders appear to affect this population, a further understanding of host genomic background variants, in conjunction with colonic mucosa dysbiosis, is necessary to determine IBS physiopathology and the effects of environmental pressures. Using a simple polygenic model, host single nucleotide polymorphisms (SNPs) and the taxonomic compositions of microbiota were compared between IBS patients and healthy subjects. As proof of concept, five IBS-Rome III patients and five healthy controls (HCs) were systematically studied. The human and bacterial intestinal metagenome of each subject was taxonomically annotated and screened for previously annotated IBS, ulcerative colitis, and Crohn's disease-associated SNPs or taxon abundance. Dietary data and fecal markers were collected and associated with the intestinal microbiome. However, more than 1,000 variants were found, and at least 76 SNPs differentiated IBS patients from HCs, as did associations with 4 phyla and 10 bacterial genera. In this study, we found elements supporting a polygenic background, with frequent variants, among the Mestizo population, and the colonic mucosal enrichment of Bacteroides, Alteromonas, Neisseria, Streptococcus, and Microbacterium, may serve as a hallmark for IBS.
Subject(s)
Bacteria/classification , Colon/microbiology , Ethnicity , Gastrointestinal Microbiome , Irritable Bowel Syndrome/genetics , Irritable Bowel Syndrome/microbiology , Multifactorial Inheritance , Adult , Bacteria/genetics , Brain/metabolism , Diet , Ethnicity/genetics , Feces/microbiology , Female , Gene Frequency , Humans , Immunity/genetics , Intestinal Mucosa/microbiology , Male , Metagenome , Middle Aged , Polymorphism, Single Nucleotide , Young AdultABSTRACT
BACKGROUND AND STUDY AIMS: Addition of a reminder program to conventional indications improves colonoscopy. The aim of this study was to evaluate the effectiveness of a short telephone call reminder (STCR) on a patient's first colonoscopy. PATIENTS AND METHODS: One day before colonoscopy, we made a STCR of <â10 minutes to 141 randomly selected patients of 258 recruited. The STCRs informed patients about the procedure date, indications for taking laxatives, and dietetic requirements. Questions were clarified only when patients asked directly. We evaluated bowel preparation, quality indicators, and patient satisfaction. Data were expressed as meanâ±âSD and percentages. Statistical differences were evaluated by Student's t and Chi squared tests; alphaâ=â0.05. All authors had access to the study data and reviewed and approved the final manuscript. RESULTS: The STCR group had better bowel preparation which was demonstrated by higher completion frequency (97.16â% vs. 82.05â%), in less time (4.52â±â3.06 vs. 5.38â±â3.03 hours) intake of laxative, and higher Boston's scale (7.66â±â2.42 vs. 5.2â±â1.65). Quality indicators of colonoscopy were better in patients that received a STCR [cecal intubation rate: 100.00â% vs. 87.18â%; polyp detection: 42.55â% vs. 9.4â%; and cecal arrival time (min): 12.09â±â3.62 vs. 15.09â±â5.02]. STCR patients were more satisfied (97.87â% vs. 55.56â%) and would repeat colonoscopy (21.99â% vs. 11.11â%). CONCLUSIONS: A simple additional step such as a STCR improves quality of bowel preparation, quality indicators, and satisfaction of patients undergoing their first colonoscopy. Clinical trial registry in Mexico City General Hospital: DI/16/107/3/108.
ABSTRACT
[This corrects the article DOI: 10.1055/s-0043-117954.].
