Performance, blood biochemistry, carcass fatty acids, antioxidant status, and HSP70 gene expressions in Japanese quails reared under high stocking density: the effects of grape seed powder and meal.

Japanese quails reared under high stocking density (SD) were evaluated for the effects of grape seed powder (GSP) and meal (GSM) supplementation on performance, blood biochemistry, thigh and breast muscle fatty acids, antioxidant status, and HSP70 gene expression. We randomly assigned 288 (15-day-old) quail chicks to six treatment groups in a factorial design (2 × 3) with four replicates, involving two density levels [160 cm2/bird (LD) and 80 cm2/bird (HD)] and three feed forms (FFs) [no supplementation, grape seed powder (3% GSP), grape seed meal (3% GSM)]. SD had a significant effect on live weight, but not on weekly feed intake, daily weight gain, and feed conversion ratio. Serum creatinine and aspartate aminotransferase levels were significantly affected by FF and SD × FF (p < 0.05). A high SD reduced the n-3/n-6 ratio of breast muscle and a significant interaction was found between FF (p < 0.001). The SD × FF interaction reduced the Σn-6 ratio in HDM's thigh muscle, whereas in LDM, the ratio increased (p < 0.01). At high SD, neither GSP nor GSM reduced biological markers of oxidative stress (p > 0.05). Compared to GSP, GSM had higher efficacy at reducing HSP70 levels related to high SD levels. Despite this, at high SD, a diet containing 3% of GSP and GSM was not effective in overcoming oxidative stress. Therefore, more studies using different doses of GSM and GSP in quail diets would be beneficial.

Effects of fermented distillers grains with solubles, partially replaced with soybean meal, on performance, blood parameters, meat quality, intestinal flora, and immune response in broiler.

This study set out to examine the effects of fermented distillers grains with solubles (DDGS) partially replaced with soybean meal on growth performance, some blood parameters, meat quality, intestinal microflora, and immune response in broilers. A total of 504 chicks were randomly allocated into 6 groups with 3 replicates. All chicks were fed with one of the following formulated diets i) basal diet based on the maize-soybean meal (C), ii) partially replaced with non-fermented DDGS (NC), iii) partially replaced fermented DDGS with B. subtilis (BS), iv) partially replaced with BS + multienzyme (BSE), v) partially replaced fermented DDGS with S. cerevisiae (SC), vi) partially replaced with SC + multienzyme (SCE). Results showed no significant difference between groups for body weight, daily weight gain (DWG), and feed intake (FI) (P > 0.05). However, feed conversion ratios (FCR) of BS, BSE, and SCE groups were significantly lower than the C and NC groups (P < 0.001). Albumin, total protein, alanine aminotransferase (P < 0.01), Total antioxidant status, aspartate aminotransferase, high-density lipoprotein, low-density lipoprotein, and uric acid (P < 0.05) were significantly affected by treatments. The meat color of the SC and SCE groups was darker after 24 h compared to the C group (P < 0.01). The highest LAB counts of ileal and cecum were observed in the BSE and SCE groups (P < 0.001). These results suggest that partial replacement of soybean meal with fermented DDGS had a positive effect on FCR without affecting DWG and FI, and combining fermented DDGS with multienzymes decreased FCR and improved immune and gut health status.