ABSTRACT
OBJECTIVE: To determine and compare the immunolocalization of functionally important antigens in human spermatozoa in an unexplained infertility (UI) group. MATERIALS AND METHODS: In this study, the sperm samples of 20 patients undergoing evaluation belonging to normozoospermic group, whose primary reason of infertility was under investigation for this purpose, were screened. CD46, CD55 and CD52, CD69, CD98, fMLP, HI307, and 80280 were stained on the spermatozoa through indirect immunofluorescence technique. RESULTS: In addition to CD46, CD55, and CD52 antigens, which are known to be localized on human spermatozoa, significant immunolocalization of several novel antigens including: CD52, CD69, CD98, fMLP, HI307, and 80280 were determined on the spermatozoa of the unexplained infertility group, possibly reflecting important roles in the pathophysiology of such unresolved clinical situations. CONCLUSION: Identification and characterization of antigens present on sperm cells is crucial for understanding of the diagnosis and treatment of unexplained infertility. Further studies were conducted to evaluate a possible correlation between the expression of these antigens and clinical outcomes in different well-defined infertility groups.
Subject(s)
Antigens/analysis , Infertility/immunology , Spermatozoa/immunology , Antigens/immunology , Antigens, CD/analysis , Antigens, Differentiation, T-Lymphocyte/analysis , Antigens, Neoplasm/analysis , CD52 Antigen , CD56 Antigen/analysis , Female , Fluorescent Antibody Technique, Indirect , Fusion Regulatory Protein-1/analysis , Glycoproteins/analysis , Humans , Lectins, C-Type/analysis , Male , Membrane Cofactor Protein/analysis , Receptors, Formyl Peptide/analysisABSTRACT
Our aim in this study was to compare reflection contrast microscopy (RCM) with transmission electron microscopy (TEM) to understand whether RCM could be used in the histopathological diagnosis of various kidney diseases as a less expensive and an easier alternative to TEM. The diagnoses of kidney pathologic lesions included Alport syndrome, thin membrane disease, Ig A nephropathy. RCM is a form of light microscope that works in the reflected mode, suitable to observe ultrathin (50-100 nm) plastic sections that is also used in TEM. Our findings showed that RCM showed similar results compared with TEM on these lesions described earlier.
Subject(s)
Kidney Diseases/diagnosis , Microscopy, Electron, Transmission/methods , Microscopy, Phase-Contrast/methods , Glomerulonephritis, IGA/diagnosis , Humans , Kidney/pathology , Kidney/ultrastructure , Kidney Tubules/pathology , Kidney Tubules/ultrastructure , Nephritis, Hereditary/diagnosis , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
PURPOSE: The objective of the present study was to determine whether neural cell adhesion molecules (NCAM; CD56) and neurothelin (CD147) are expressed by adontogenic cells in the ameloblastoma. MATERIALS AND METHODS: Frozen sections from ameloblastoma specimens (n = 7) were stained with monoclonal antibody-recognizing CD56 and CD147 molecules using indirect immunoperoxidase and indirect immunofluorescent techniques. RESULTS: CD56 and CD147 molecules were strongly expressed by the peripheral columnar cells of the tumor nests. Neurothelin reactivity was also present in the stellate reticulum of the nests and in some stromal components. CONCLUSIONS: The presence of these antigens in ameloblastoma supports the classic view about the neural crest origin of cells giving use to this tumor.