[Le miR-224-5p régulé sert de biomarqueur pour l'insuffisance hépatique aiguë pédiatrique et régule l'inflammation en modulant ZBTB20]. / Upregulated miR-224-5p served as a biomarker for pediatric acute liver failure and regulate inflammation by modulating ZBTB20.

Pediatric acute liver failure (PALF) is a severe liver dysfunction with complex pathological mechanisms and rapid development. MiRNAs have been identified as promising biomarkers for human disease screening and monitoring. This study focused on evaluating the clinical significance of miR-224-5p in PALF and revealing its potential molecular mechanism in regulating liver cell injury. This study enrolled 103 children with PALF and 55 healthy children without liver diseases. Serum miR-224-5p levels were compared between the two groups, and their clinical significance was estimated by analyzing the correlation with clinicopathological features and outcomes of PALF children. In vitro, a normal liver cell was treated with lipopolysaccharide (LPS), and cell growth and inflammation were assessed by CCK8 and ELISA assay. Upregulated miR-224-5p in PALF showed significance in screening PALF children from healthy children with the sensitivity and specificity of 78.64% and 84.47%, respectively. Increasing serum miR-224-5p in PALF children was closely associated with increasing prothrombin time, alanine transaminase, international normalized ratio, total bilirubin, ammonia, and aspartic transaminase and decreasing albumin of PALF children. MiR-224-5p was also identified as a risk factor for adverse outcomes in children with PALF. In LPS-treated liver cells, miR-224-5p could negatively regulate ZBTB20, and silencing miR-224-5p could alleviate the inhibited cell growth and promoted inflammation by LPS, which was reversed by ZBTB20 knockdown. Increasing miR-224-5p distinguished PALF children, predict severe disease development and risk of adverse prognosis. miR-224-5p also reguled LPS-induced liver cell injury via negatively regulating ZBTB20.

Effect of Yangxin Huoxue Jiedu recipe on inflammatory factors and oxidative stress on viral myocarditis in children.

OBJECTIVE: This observation purposed to investigate the effect of the Yangxin Huoxue Jiedu formula on children with viral myocarditis and its effect on inflammatory factors and oxidative response. MATERIALS AND METHODS: A total of 121 children with viral myocarditis were randomly divided into two groups, namely the control group (N = 60) and the traditional Chinese medicine group (N = 61). The control group was mainly treated with routine therapy, while the traditional Chinese medicine group was treated with Yangxin Huoxue Jiedu recipes based on the control group. The creatine kinase, creatine kinase myocardial isoenzyme, aspartate aminotransferase, lactic dehydrogenase, hydroxybutyrate dehydrogenase, cardiac troponin I, brain natriuretic peptide, interleukin-6, interleukin-8, and tumour necrosis factor-alpha, superoxide dismutase and malondialdehyde in viral myocarditis patients were tested to estimate the myocardial function, inflammation, and oxidative situation. RESULTS: After Yangxin Huoxue Jiedu treatment, 15 cases were recovered, 20 were excellent, and 21 were effective, which had a significant difference from the control group. The concentration of creatine kinase, creatine kinase myocardial isoenzyme, aspartate aminotransferase, lactic dehydrogenase, hydroxybutyrate dehydrogenase, cardiac troponin I and brain natriuretic peptide was decreased in the traditional Chinese medicine group. The levels of interleukin-6, interleukin-8, and tumour necrosis factor-alpha in the traditional Chinese medicine group were significantly lower than those in the control group. Superoxide dismutase was higher and malondialdehyde was lower than those in the control group. CONCLUSION: The use of Yangxin Huoxue Jiedu in the treatment of viral myocarditis has a definite clinical effect, which could improve myocardial function, reduce body inflammation, and promote oxidative recovery.

Resource Utilization of Red Mud from the Solid Waste of Aluminum Industry Used in Geothermal Wells.

It is difficult for the commonly used Class G oil well cement to withstand the high-temperature environment of geothermal wells, and it is easy to deteriorate the mechanical properties and damage the integrity of the cement sheath. Industrial solid waste red mud can be used as supplementary cementing materials (SCMs) to improve its mechanical properties at high temperatures. In addition, compared to Class G oil well cement, high belite cement (HBC) has lower energy consumption and better mechanical properties at high temperatures. In this study, the mechanical properties of HBC as a gel material and quartz sand and red mud as SCMs were studied at high temperatures. The ratio of HBC to SCMs and the ratio of quartz sand to red mud in SCMs were optimized using the response surface method (RSM). The response surface was established using the three-level factorial design model, which fit well with the experimental data. The optimization results show that the best mass ratio of SCMs/HBC is 37.5% and that the best quality ratio of quartz sand/red mud is 9 under the curing conditions of 180 °C. However, the best mass ratio of SCMs/HBC is 49.3%, and the best quality ratio of quartz sand/red mud is 7 under 220 °C. With the addition of SCMs, the silicon-to-calcium ratio of HBC hydration products decreases, and high-temperature-stable xonotlite and tobermorite can be formed. After adding SCMs, the cement sample is denser without obvious cracks.

Resource utilization from solid waste originated from oil-based shale drilling cutting during shale gas development.

With the large-scale development of shale gas, oil-based drilling fluids are widely used, generating significant amounts of solid wastes from oil-based shale drilling cutting (OBSDC). These solid wastes are biologically toxic and are difficult to degrade. The current treatment methods do not meet the requirements for oily sludge. This study begins with pyrolysis of OBSDC in order to use it as an external admixture for preparing cement slurry for resource utilization. The research results showed that when the OBSDC content was increased to 35%, the mechanical properties of developed cement were favourable. Evaluating the cement sheath integrity showed that the OBSDC cement met the subsequent production requirements for a casing with an internal pressure of 50.01 MPa, applicable for cementing surface casings as well as technical casings. The active SiO2 and feldspar in OBSDC after pyrolysis promoted the hydration reaction of cement and accelerated the crystallization of C-S-H, which in turn complemented the cement mechanical properties. When OBSDC was in the proper dosage range, the particle gradation characteristics further optimized the pore structure of the cement matrix and increased the cement strength.