ABSTRACT
Hepatocellular carcinoma is one of the most common malignant tumors worldwide; it is estimated that there were 782,000 new cases in 2012. MicroRNAs (miRNAs) play an important role in carcinogenesis by regulating oncogenes and tumor suppressors. We investigated the role of miR-146a, miR-196a2, and miR-499 polymorphisms in the risk of hepatocellular carcinoma in a Chinese population. Hepatocellular carcinoma patients (175) and healthy controls (302) were recruited between April 2013 and March 2015. Genotype analysis of miR-146a, miR-196a2, and miR-499 polymorphisms was carried out by polymerase chain reaction-restriction fragment length polymorphism. There was a significant difference between the genotype distribution of miR-196a2 in hepatocellular carcinoma patients and controls (X2 = 17.23, P < 0.001). CG and GG miR-146a genotypes significantly elevated the risk of hepatocellular carcinoma compared with the CC genotype, with adjusted ORs (95%CI) of 3.05 (1.07-8.70) and 4.96 (1.64-14.97), respectively. In the recessive model, the CG + GG genotype had a 3.75-fold risk of hepatocellular carcinoma compared with the CC genotype, with an adjusted OR (95%CI) of 3.75 (1.39-10.11). However, no significant association was observed between miR-196a2 and miR-499 variants and risk of hepatocellular carcinoma in the co-dominant, dominant, and recessive models. The miR-146a polymorphism is a G to C substitution that causes a mismatch in the stem-loop of miRNA, which influences how the expression and transcriptional regulation of miRNA affects its target genes. Our study revealed that the GG and CG genotypes of miR-146a increased the risk of hepatocellular carcinoma in the Chinese population.
Subject(s)
Carcinoma, Hepatocellular/genetics , Liver Neoplasms/genetics , MicroRNAs/genetics , Polymorphism, Single Nucleotide , Aged , Case-Control Studies , Female , Gene Frequency , Genotype , Humans , Male , Middle AgedABSTRACT
We conducted this case-control study to investigate the genetic role of IL-1ß+3954C/T, IL-6-174G/C, IL-6-572C/G, IL-10-1082A/G, and IL-10-819C/T in the development of coronary artery disease (CAD) in a Chinese population. Polymorphisms IL-1ß+3954C/T, IL-6-174G/C, IL-6-572C/G, IL-10-1082A/G, and IL-10-819C/T were determined by polymerase chain reaction restriction fragment length polymorphism assay. The CAD patients were more likely to be cigarette smokers, have a history of hypertension, have a higher value of total cholesterol, triglycerides, and low-density lipoprotein cholesterol, and have a lower value of high-density lipoprotein cholesterol. Conditional logistic regression analyses showed that the CC genotype of IL-6-174G/C was significantly associated with increased risk of CAD [odds ratio (OR) = 2.99, 95% confidence interval (95%CI) = 1.56-6.00]. Moreover, the GG genotype of IL-6-572C/G was correlated with increased risk of CAD (OR = 1.99, 95%CI = 1.25-3.19). We found that IL-6-174G/C and IL-6-572C/G gene polymorphisms are associated with an increased risk of CAD.
Subject(s)
Coronary Artery Disease/genetics , Interleukin-6/genetics , Adult , Aged , Aged, 80 and over , Case-Control Studies , Cholesterol, HDL/metabolism , Coronary Artery Disease/metabolism , Female , Genetic Association Studies , Genetic Predisposition to Disease , Humans , Interleukin-10/genetics , Interleukin-1beta/genetics , Interleukins/genetics , Lipoproteins, LDL/metabolism , Male , Middle Aged , Odds Ratio , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Risk FactorsABSTRACT
We conducted a case-control study to assess the relation-ship between rs35767, rs2288377, and rs5742612 insulin-like growth factor-1 (IGF-1) and osteoporosis risk in a Chinese female population. The genotypes of rs35767, rs2288377, and rs5742612 of IGF-1 were determined by polymerase chain reaction-restriction fragment length polymorphism. Patients with osteoporosis were more likely to have drinking and smoking habits and have lower bone mineral density in the L2-L4 vertebrae, femoral neck, and total hip. According to conditional regression analysis, individuals carrying the TT genotype of rs35767 had an increased risk of osteoporosis, with an adjusted odds ratio (95% confidence interval) of 2.29 (1.35-4.97). In conclusion, our results sug-gest that the TT genotype of IGF-I rs35767 was associated with an in-creased risk of osteoporosis, suggesting that this polymorphism can be used as a predictive factor for osteoporosis risk.
Subject(s)
Genetic Association Studies , Genetic Predisposition to Disease , Insulin-Like Growth Factor I/genetics , Osteoporosis/genetics , Polymorphism, Single Nucleotide/genetics , Case-Control Studies , Demography , Female , Gene Frequency , Humans , Male , Middle Aged , Odds Ratio , Risk FactorsABSTRACT
Gastric cancer is the fourth most common cancer worldwide and the leading cause of tumor-related death in China. Gastric cancer is a heterogeneous disease and therefore requires different treatments based on the subtype. We describe a patient who had gastric cancer with liver metastases. Biopsy and tumor analysis using the HercepTest revealed a human epidermal growth factor receptor 2 (HER2)-positive adenocarcinoma as confirmed by fluorescence in situ hybridization. The patient was treated with a regimen of trastuzumab, oxaliplatin, and S-1 (six cycles). When positron emission tomography findings suggested that the metastases had resolved, the patient underwent surgery. Histopathologically, no cancer cells were observed in the resected hepatic tissue. The patient underwent tumor resection surgery, during which the tumor and gastric lymph nodes with lesions were removed. The patient has remained disease-free for 3 months. Therefore, trastuzumab may be an effective agent in the chemotherapeutic treatment of liver metastases in patients with HER2-positive gastric adenocarcinoma.
Subject(s)
Antibodies, Monoclonal, Humanized/administration & dosage , Antineoplastic Agents/administration & dosage , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Liver Neoplasms/secondary , Stomach Neoplasms/drug therapy , Stomach Neoplasms/surgery , Drug Combinations , Female , Humans , Liver Neoplasms/drug therapy , Liver Neoplasms/genetics , Liver Neoplasms/surgery , Middle Aged , Organoplatinum Compounds/administration & dosage , Oxaliplatin , Oxonic Acid/administration & dosage , Receptor, ErbB-2/genetics , Stomach Neoplasms/genetics , Stomach Neoplasms/pathology , Tegafur/administration & dosage , Trastuzumab , Treatment OutcomeABSTRACT
Polymerase chain reaction-ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR-RFLP) analysis and sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and NADH dehydrogenase 1 (ND1) genes were used to characterize 33 Echinococcus granulosus isolates collected from different regions and hosts in Argentina, and to determine which genotypes occurred in humans with cystic hydatid disease. The results of the study demonstrated the presence of at least 4 distinct genotypes; the common sheep strain (G1) in sheep from Chubut Province and in humans from Río Negro Province, the Tasmanian sheep strain (G2) in sheep and 1 human from Tucumán Province, the pig strain (G7) in pigs from Santa Fe Province and the carnel strain (G6) in humans from Río Negro and Buenos Aires Provinces. The finding that pigs harboured the pig strain and the occurrence of the Tasmanian sheep strain has considerable implications for the implementation of hydatid control programmes due to the shorter maturation time of both strains in dogs compared with the common sheep strain. Furthermore, this is the first report of the presence of the G2 and G6 genotypes in humans which may also have important consequences for human health.