ABSTRACT
To determine the prevalence and clinical features of olfactory and taste disorders among coronavirus disease 2019 (COVID-19) patients in China. A cross-sectional study was performed in Wuhan from April 3, 2020 to April 15, 2020. A total of 187 patients with confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) completed face-to-face interviews or telephone follow-ups. We found that the prevalence of olfactory and taste disorders was significantly lower in the Chinese cohort than in foreign COVID-19 cohorts. Females were more prone to olfactory and taste disorders. In some patients, olfactory and taste disorders precede other symptoms and can be used as early screening and warning signs.
Subject(s)
COVID-19/complications , Olfaction Disorders/etiology , Smell , Taste Disorders/etiology , Taste , Adolescent , Adult , Aged , Aged, 80 and over , China/epidemiology , Cross-Sectional Studies , Female , Humans , Male , Middle Aged , Olfaction Disorders/epidemiology , Prevalence , SARS-CoV-2 , Sex Factors , Taste Disorders/epidemiology , Young AdultABSTRACT
Aberrant microRNA (miRNA) expression has been linked to cancer development. In this study, we aimed to investigate whether the anticancer effect of miRNA2993p on laryngeal cancer Hep2 cells is mediated through targeting human telomerase reverse transcriptase (hTERT). The expression of miR2993p in laryngeal cancer Hep2 cells and human osteosarcoma U2OS cells was quantified by stemloopmediated reverse transcription quantitative polymerase chain reaction. miR2993p mimic was transfected into Hep2 cells to induce overexpression of miR2993p. A CCK8 assay was performed to identify the effects of miR2993p overexpression on the proliferation of Hep2 cells. Western blot analysis was carried out to determine the expression of hTERT protein. A significant decrease was noted in the expression of miR2993p in Hep2 cells compared with that of U2OS cells (P<0.05). Overexpression of miR2993p resulted in a notable inhibition of cellular proliferation (P<0.05), as well as downregulation of hTERT mRNA and protein in Hep2 cells (P>0.05). The expression of miR2993p is downregulated in human laryngeal cancer Hep2 cells. Overexpression of miR2993p inhibits Hep2 cell growth by targeting the 3'untranslated region of hTERT mRNA.
Subject(s)
MicroRNAs/metabolism , Telomerase/metabolism , 3' Untranslated Regions , Base Sequence , Cell Line, Tumor , Cell Proliferation , Down-Regulation , HeLa Cells , Humans , Laryngeal Neoplasms/genetics , Laryngeal Neoplasms/pathology , MicroRNAs/antagonists & inhibitors , MicroRNAs/genetics , Oligonucleotides, Antisense/metabolism , RNA, Messenger/metabolism , Sequence Alignment , Telomerase/chemistry , Telomerase/geneticsABSTRACT
The study on determining the concentration of coating solution attaching to honeycomb denuder was conducted, from 1 July to 31 August, 2013, at the roof of Lihua building at Nankai University in Tianjin. The results of experiment showed that the optimized concentration of sodium carbonate coated on the honeycomb denuder was 3%, and the optimized concentration of citric acid was 6%. The contrast sampling results of PM2.5 between honeycomb denuder system and conventional method showed that 86% of the concentration of PM2.5 samples obtained by honeycomb denuder system were less than those obtained by conventional method, the main reasons may include that: (1) the majority of acid/alkaline gases were removed, so they could neither react with the enriched particles on the sampling membrane nor be adsorbed on particles; (2) parts of the particles were captured by the denuder during sampling; (3) the removal of acid/alkaline gases disturbed the state of equilibrium between gas- and particle-phases which may lead to the volatilization of some particles.
