Challenges and opportunities in commercializing whole-cell bioreporters in environmental application.

Since the initial introduction of whole-cell bioreporters (WCBs) nearly 30 years ago, their high sensitivity, selectivity, and suitability for on-site detection have rendered them highly promising for environmental monitoring, medical diagnosis, food safety, biomanufacturing, and other fields. Especially in the environmental field, the technology provides a fast and efficient way to assess the bioavailability of pollutants in the environment. Despite these advantages, the technology has not been commercialized. This lack of commercialization is confusing, given the broad application prospects of WCBs. Over the years, numerous research papers have focused primarily on enhancing the sensitivity and selectivity of WCBs, with little attention paid to their wider commercial applications. So far, there is no a critical review has been published yet on this topic. Therefore, in this article we critically reviewed the research progress of WCBs over the past three decades, assessing the performance and limitations of current systems to understand the barriers to commercial deployment. By identifying these obstacles, this article provided researchers and industry stakeholders with deeper insights into the challenges hindering market entry and inspire further research toward overcoming these barriers, thereby facilitating the commercialization of WCBs as a promising technology for environmental monitoring.

Enhancement of nutrient use efficiency with biochar and wood vinegar: A promising strategy for improving soil productivity.

BACKGROUND: The co-application of biochar and wood vinegar has demonstrated the potential to enhance premium crop production. The present study reveals the effects of co-applying rice husk biochar and wood vinegar (both foliar and soil application) on soil properties and the growth of Chinese cabbage (Brassica chinensis L.) in a two-season pot experiment. RESULTS: The soil pH, electrical conductivity and dissolved organic carbon contents in combination treatments of wood vinegar and biochar were increased more when wood vinegar was applied to soils rather than to leaves, and the parameters were observed to surpass those for chemical fertilizer treatments. The biomass of Chinese cabbage shoots was significantly increased by 60.8- and 27.3-fold in the combined treatments compared to the control when 1% wood vinegar was sprayed to the leaves (WF1) in 2022 and 2023, respectively. Higher contents of vitamin C, soluble protein and soluble sugar were also observed in the combined wood vinegar and biochar treatments compared to chemical fertilizer treatments and the control; for example, the vitamin C content of plant shoot in WF1 was 21.3 times that of the control. The yield and quality of plants were decreased across all treatments in 2023 compared to 2022 but the combination treatments still displayed superiority. CONCLUSION: The co-application of wood vinegar and biochar enhances the growth and improve the quality of Chinese cabbage through improving the soil properties and plant photosynthesis. Moreover, the foliage application of wood vinegar is more preferable compared to soil application. © 2024 Society of Chemical Industry.

The interaction between biochar and earthworms: Revealing the potential ecological risks of biochar application and the feasibility of their co-application.

Biochar's interaction with soil-dwelling organisms, particularly earthworms, is crucial in ensuring the effective and secure utilization of biochar in the soil. This review introduces the application of biochar in soil, summarizes how earthworms respond to biochar-amended soil and the underlying factors that can influence their response, discusses the synergistic and antagonistic impacts of earthworm activity on the efficacy of biochar, and considers the feasibility of applying them together. A review of existing research has identified uncertainty in the effect of biochar exposure on earthworms, with biochar derived from animal wastes, produced at higher pyrolysis temperatures, and used at higher doses of biochar having more negative effects on earthworms. Habitat modification, toxicity release, particle effects, and contaminant immobilization are underlying factors in how biochar affects earthworm indicators. While biochar in contaminated soils may alleviate the stress of pollutants on earthworms by decreasing their bioaccumulation, this remedial effect is not always effective. Additionally, earthworm bioturbation can enhance the migration, fragmentation, and oxidation of biochar, while also stimulating extracellular enzymes that convert biochar into 'vermichar'. Earthworms and biochar can synergize well to improve soil fertility and remediate soil organic pollution, yet exhibit contrasting roles in soil C sequestration and immobilizing heavy metals in soil. These findings highlight both the advantages and risks of their co-application. Therefore, when considering the use of biochar alone or with earthworms, it is crucial to thoroughly assess its potential ecotoxicity on earthworms and other soil organisms, as well as the influence of bioturbation, such as that caused by earthworms, on the effectiveness of biochar.

A critical review of biochar for the remediation of PFAS-contaminated soil and water.

Per- and polyfluoroalkyl substances (PFAS) present significant environmental and health hazards due to their inherent persistence, ubiquitous presence in the environment, and propensity for bioaccumulation. Consequently, the development of efficacious remediation strategies for soil and water contaminated with PFAS is imperative. Biochar, with its unique properties, has emerged as a cost-effective adsorbent for PFAS. Despite this, a comprehensive review of the factors influencing PFAS adsorption and immobilization by biochar is lacking. This narrative review examines recent findings indicating that the application of biochar can effectively immobilize PFAS, thereby mitigating their environmental transport and subsequent ecological impact. In addition, this paper reviewed the sorption mechanisms of biochar and the factors affecting its sorption efficiency. The high effectiveness of biochars in PFAS remediation has been attributed to their high porosity in the right pore size range (>1.5 nm) that can accommodate the relatively large PFAS molecules (>1.02-2.20 nm), leading to physical entrapment. Effective sorption requires attraction or bonding to the biochar framework. Binding is stronger for long-chain PFAS than for short-chain PFAS, as attractive forces between long hydrophobic CF2-tails more easily overcome the repulsion of the often-anionic head groups by net negatively charged biochars. This review summarizes case studies and field applications highlighting the effectiveness of biochar across various matrices, showcasing its strong binding with PFAS. We suggest that research should focus on improving the adsorption performance of biochar for short-chain PFAS compounds. Establishing the significance of biochar surface electrical charge in the adsorption process of PFAS is necessary, as well as quantifying the respective contributions of electrostatic forces and hydrophobic van der Waals forces to the adsorption of both short- and long-chain PFAS. There is an urgent need for validation of the effectiveness of the biochar effect in actual environmental conditions through prolonged outdoor testing.

Biodegradable microplastics pose greater risks than conventional microplastics to soil properties, microbial community and plant growth, especially under flooded conditions.

Biodegradable plastics (bio-plastics) are often viewed as viable option for mitigating plastic pollution. Nevertheless, the information regarding the potential risks of microplastics (MPs) released from bio-plastics in soil, particularly in flooded soils, is lacking. Here, our objective was to investigate the effect of polylactic acid MPs (PLA-MPs) and polyethylene MPs (PE-MPs) on soil properties, microbial community and plant growth under both non-flooded and flooded conditions. Our results demonstrated that PLA-MPs dramatically increased soil labile carbon (C) content and altered its composition and chemodiversity. The enrichment of labile C stimulated microbial N immobilization, resulting in a depletion of soil mineral nitrogen (N). This specialized environment created by PLA-MPs further filtered out specific microbial species, resulting in a low diversity and simplified microbial community. PLA-MPs caused an increase in denitrifiers (Noviherbaspirillum and Clostridium sensu stricto) and a decrease in nitrifiers (Nitrospira, MND1, and Ellin6067), potentially exacerbating the mineral N deficiency. The mineral N deficit caused by PLA-MPs inhibited wheatgrass growth. Conversely, PE-MPs had less effect on soil ecosystems, including soil properties, microbial community and wheatgrass growth. Overall, our study emphasizes that PLA-MPs cause more adverse effect on the ecosystem than PE-MPs in the short term, and that flooded conditions exacerbate and prolong these adverse effects. These results offer valuable insights for evaluating the potential threats of bio-MPs in both uplands and wetlands.

Type VII secretion system extracellular protein B targets STING to evade host anti-Staphylococcus aureus immunity.

Staphylococcus aureus (S. aureus) can evade antibiotics and host immune defenses by persisting within infected cells. Here, we demonstrate that in infected host cells, S. aureus type VII secretion system (T7SS) extracellular protein B (EsxB) interacts with the stimulator of interferon genes (STING) protein and suppresses the inflammatory defense mechanism of macrophages during early infection. The binding of EsxB with STING disrupts the K48-linked ubiquitination of EsxB at lysine 33, thereby preventing EsxB degradation. Furthermore, EsxB-STING binding appears to interrupt the interaction of 2 vital regulatory proteins with STING: aspartate-histidine-histidine-cysteine domain-containing protein 3 (DHHC3) and TNF receptor-associated factor 6. This persistent dual suppression of STING interactions deregulates intracellular proinflammatory pathways in macrophages, inhibiting STING's palmitoylation at cysteine 91 and its K63-linked ubiquitination at lysine 83. These findings uncover an immune-evasion mechanism by S. aureus T7SS during intracellular macrophage infection, which has implications for developing effective immunomodulators to combat S. aureus infections.

Pretreatment of wastepaper with an aqueous solution of amino acid-derived ionic liquid for biochar production as adsorbent.

The carbonization of lignocellulosic biomass with ionic liquids (ILs) are considered as an advantageous approach for the preparation of carbonaceous materials. The commonly used imidazolium and pyridinium based ILs have drawbacks such as toxicity, resistance to biodegradation, high cost and viscosity. These issues can be mitigated by diluting ILs with water, although excessive water content above 1 wt% can reduce the solubility of biomass. This research aims to investigate the potential of pretreating wastepaper with a "fully green" ILs, amino acid-based IL with high water content, followed by pyrolysis without IL, in enhancing the properties of biochar. For this purpose, the paper was treated with an aqueous solution of IL cysteine nitrate ([Cys][NO3]), and the IL was not involved in the pyrolysis process to prevent the formation of secondary gaseous pollutants. The findings revealed that the hemicellulose and mineral filler in the paper were eliminated during pretreatment, leading to higher carbon content but lower oxygen content. As a result, the biochar exhibited micropores of 0.42 cm3g-1 and a specific surface area of 1011.21 m2 g-1. The biochar demonstrated high adsorption capacities for Cd2+, enrofloxacin, bisphenol A, ciprofloxacin, and tetracycline, with values of 45.20 mg g-1, 49.82 mg g-1, 49.90 mg g-1, 49.88 mg g-1, and 49.65 mg g-1, respectively. The proposed mechanism for the adsorption of enrofloxacin by the biochar primarily involves physical adsorption such as pore filling and electrostatic interactions, along with chemical adsorption facilitated by graphitic nitrogen.

TEA domain transcription factor 1(TEAD1) induces cardiac fibroblasts cells remodeling through BRD4/Wnt4 pathway.

Cardiac fibroblasts (CFs) are the primary cells tasked with depositing and remodeling collagen and significantly associated with heart failure (HF). TEAD1 has been shown to be essential for heart development and homeostasis. However, fibroblast endogenous TEAD1 in cardiac remodeling remains incompletely understood. Transcriptomic analyses revealed consistently upregulated cardiac TEAD1 expression in mice 4 weeks after transverse aortic constriction (TAC) and Ang-II infusion. Further investigation revealed that CFs were the primary cell type expressing elevated TEAD1 levels in response to pressure overload. Conditional TEAD1 knockout was achieved by crossing TEAD1-floxed mice with CFs- and myofibroblasts-specific Cre mice. Echocardiographic and histological analyses demonstrated that CFs- and myofibroblasts-specific TEAD1 deficiency and treatment with TEAD1 inhibitor, VT103, ameliorated TAC-induced cardiac remodeling. Mechanistically, RNA-seq and ChIP-seq analysis identified Wnt4 as a novel TEAD1 target. TEAD1 has been shown to promote the fibroblast-to-myofibroblast transition through the Wnt signalling pathway, and genetic Wnt4 knockdown inhibited the pro-transformation phenotype in CFs with TEAD1 overexpression. Furthermore, co-immunoprecipitation combined with mass spectrometry, chromatin immunoprecipitation, and luciferase assays demonstrated interaction between TEAD1 and BET protein BRD4, leading to the binding and activation of the Wnt4 promoter. In conclusion, TEAD1 is an essential regulator of the pro-fibrotic CFs phenotype associated with pathological cardiac remodeling via the BRD4/Wnt4 signalling pathway.

Double Advantages of Nutrients and Biostimulants Derived from Sewage Sludge by Alkaline Thermal Hydrolysis Process for Agricultural Use: Quality Promotion of Soil and Crop.

Low-carbon alkaline thermal hydrolysis of sewage sludge for the production of high-quality plant-growth-promoting nutrients and biostimulants is a growing concern for sludge resource utilization in agriculture. Thus, this study aims to investigate functional characteristics and soil biochemical effects of sewage sludge-derived nutrients and biostimulants (SS-NB). The content of heavy metals in SS-NB decreased by 47.39-100%, and an increase in soil protease, invertase, and soil nutrient utilization rates are observed in SS-NB groups. SS-NB substituted for chemical fertilizer increased the diversity and evenness of microbial community and reduced the abundance of the soil-borne bacterial genus Arthrobacter. The dominant community of SS-NB100 group is mainly enriched in Microvirga, Ensifer, Novosphingobium, Bosea and Ellin6055, which are principally beneficial symbiotic bacteria of plants and participated in C and N cycles. Moreover, SS-NB reduced the accumulation of Ktedonobacteria and Nitrosospira, which are involved in the production of CO2 and N2O, and also enhanced the coordination of soil microorganisms with enzyme activities and nutrient utilization rate. In conclusion, the results suggest that SS-NB exerts a positive effect on reducing greenhouse gas emissions and preventing soil-borne diseases, and can further enhance collaboration with soil enzyme activity and soil nutrient utilization by stimulating soil microorganisms.

Antifungal efficacy of biogenic waste derived colloidal/nanobiochar against Colletotrichum gloeosporioides species complex.

Anthracnose caused by Colletotrichum spp. usually resulting in significant postharvest losses in the banana production chain. This study investigated the inhibitory effect of corn cob colloidal/nanobiochar (CCN) and Gliricidia sepium wood colloidal/nanobiochar (GCN) on the Colletotrichum gloeosporioides species complex. The CCN and GCN materials were synthesized and thoroughly characterized using various techniques, including UV-Vis and Fluorescence spectroscopy. Then after the fungal growth was examined on Potato Dextrose Agar (PDA) media supplemented with different CCN and GCN concentrations of 0.4 - 20 g/L and CCN and GCN with zeolite at various weight percentages of 10% to 50% w/w. Results from the characterization revealed that CCN exhibited a strong UV absorbance peak value of 0.630 at 203 nm, while GCN had a value of 0.305 at 204 nm. In terms of fluorescence emission, CCN displayed a strong peak intensity of 16,371 at 412 nm, whereas GCN exhibited a strong peak intensity of 32,691 at 411 nm. Both CCN and GCN, at concentrations ranging from 1 to 8 and 0.4 - 20 g/L, respectively, displayed notable reductions in mycelial densities and inhibited fungal growth compared to the control. Zeolite incorporation further enhanced the antifungal effect. To the best of our knowledge, this is the first study to demonstrate the promising potential of colloidal/nanobiochar in effectively controlling anthracnose disease. The synthesized CCN and GCN demonstrate promising antifungal potential against Colletotrichum gloeosporioides species complex, offering the potential for the development of novel and effective antifungal strategies for controlling anthracnose disease in Musa spp.

Environmental behaviors and toxic mechanisms of engineered nanomaterials in soil.

Engineered nanomaterials (ENMs) are inevitably released into the environment with the exponential application of nanotechnology. Parts of ENMs eventually accumulate in the soil environment leading to potential adverse effects on soil ecology, crop production, and human health. Therefore, the safety application of ENMs on soil has been widely discussed in recent years. More detailed safety information and potential soil environmental risks are urgently needed. However, most of the studies on the environmental effects of metal-based ENMs have been limited to single-species experiments, ecosystem processes, or abiotic processes. The present review formulated the source and the behaviors of the ENMs in soil, and the potential effects of single and co-exposure ENMs on soil microorganisms, soil fauna, and plants were introduced. The toxicity mechanism of ENMs to soil organisms was also reviewed including oxidative stress, the release of toxic metal ions, and physical contact. Soil properties affect the transport, transformation, and toxicity of ENMs. Toxic mechanisms of ENMs include oxidative stress, ion release, and physical contact. Joint toxic effects occur through adsorption, photodegradation, and loading. Besides, future research should focus on the toxic effects of ENMs at the food chain levels, the effects of ENMs on plant whole-lifecycle, and the co-exposure and long-term toxicity effects. A fast and accurate toxicity evaluation system and model method are urgently needed to solve the current difficulties. It is of great significance for the sustainable development of ENMs to provide the theoretical basis for the ecological risk assessment and environmental management of ENMs.

Antibody-antibiotic conjugate targeted therapy for orthopedic implant-associated intracellular S. aureus infections.

INTRODUCTION: Treating orthopedic implant-associated infections, especially those caused by Staphylococcus aureus (S. aureus), remains a significant challenge. S. aureus has the ability to invade host cells, enabling it to evade both antibiotics and immune responses during infection, which may result in clinical treatment failures. Therefore, it is critical to identify the host cell type of implant-associated intracellular S. aureus infections and to develop a strategy for highly targeted delivery of antibiotics to the host cells. OBJECTIVES: Introduced an antibody-antibiotic conjugate (AAC) for the targeted elimination of intracellular S. aureus. METHODS: The AAC comprises of a human monoclonal antibody (M0662) directly recognizes the surface antigen of S. aureus, Staphylococcus protein A, which is conjugated with vancomycin through cathepsin-sensitive linkers that are cleavable in the proteolytic environment of the intracellular phagolysosome. AAC, vancomycin and vancomycin combined with AAC were used in vitro intracellular infection and mice implant infection models. We then tested the effect of AAC in vivo and in vivo by fluorescence imaging, in vivo imaging, bacterial quantitative analysis and bacterial biofilm imaging. RESULTS: In vitro, it was observed that AAC captured extracellular S. aureus and co-entered the cells, and subsequently released vancomycin to induce rapid elimination of intracellular S. aureus. In the implant infection model, AAC significantly improved the bactericidal effect of vancomycin. Scanning electron microscopy showed that the application of AAC effectively blocked the formation of bacterial biofilm. Further histochemical and micro-CT analysis showed AAC significantly reduced the level of bone marrow density (BMD) and bone volume fraction (BV/TV) reduction caused by bacterial infection in the distal femur of mice compared to vancomycin treatment alone. CONCLUSIONS: The application of AAC in an implant infection model showed that it significantly improved the bactericidal effects of vancomycin and effectively blocked the formation of bacterial biofilms, without apparent toxicity to the host.

Downregulation of apoptotic repressor AVEN exacerbates cardiac injury after myocardial infarction.

Myocardial infarction (MI) is a leading cause of heart failure (HF), associated with morbidity and mortality worldwide. As an essential part of gene expression regulation, the role of alternative polyadenylation (APA) in post-MI HF remains elusive. Here, we revealed a global, APA-mediated, 3' untranslated region (3' UTR)-lengthening pattern in both human and murine post-MI HF samples. Furthermore, the 3' UTR of apoptotic repressor gene, AVEN, is lengthened after MI, contributing to its downregulation. AVEN knockdown increased cardiomyocyte apoptosis, whereas restoration of AVEN expression substantially improved cardiac function. Mechanistically, AVEN 3' UTR lengthening provides additional binding sites for miR-30b-5p and miR-30c-5p, thus reducing AVEN expression. Additionally, PABPN1 (poly(A)-binding protein 1) was identified as a potential regulator of AVEN 3' UTR lengthening after MI. Altogether, our findings revealed APA as a unique mechanism regulating cardiac injury in response to MI and also indicated that the APA-regulated gene, AVEN, holds great potential as a critical therapeutic target for treating post-MI HF.

IDAF: Iterative Dual-Scale Attentional Fusion Network for Automatic Modulation Recognition.

Recently, deep learning models have been widely applied to modulation recognition, and they have become a hot topic due to their excellent end-to-end learning capabilities. However, current methods are mostly based on uni-modal inputs, which suffer from incomplete information and local optimization. To complement the advantages of different modalities, we focus on the multi-modal fusion method. Therefore, we introduce an iterative dual-scale attentional fusion (iDAF) method to integrate multimodal data. Firstly, two feature maps with different receptive field sizes are constructed using local and global embedding layers. Secondly, the feature inputs are iterated into the iterative dual-channel attention module (iDCAM), where the two branches capture the details of high-level features and the global weights of each modal channel, respectively. The iDAF not only extracts the recognition characteristics of each of the specific domains, but also complements the strengths of different modalities to obtain a fruitful view. Our iDAF achieves a recognition accuracy of 93.5% at 10 dB and 0.6232 at full signal-to-noise ratio (SNR). The comparative experiments and ablation studies effectively demonstrate the effectiveness and superiority of the iDAF.

Toxins from harmful algal blooms: How copper and iron render chalkophore a predictor of microcystin production.

Research on harmful algal blooms has focused on macronutrients, yet recent research increasingly indicates that understanding micronutrient roles is also important in the development of effective environmental management interventions. Here, we report results on metallophore production from mesocosms amended with copper and iron (enzymatic co-factors in photosynthetic electron transport) to probe questions of how cyanobacteria navigate the divide between copper nutrition, copper toxicity, and issues with iron bioavailability. These experiments utilized Microcystis, Chlorella and Desmodesmus spp., in mono- and mixed-cultures in lake water from a large, hypereutrophic lake (Taihu, China). To initiate experiments, copper and iron amendments were added to mesocosms containing algae that had been acclimated to achieve a state of copper and iron limitation. Mesocosms were analyzed over time for a range of analytes including algal growth parameters, algal assemblage progression, copper/iron concentrations and biomolecule production of chalkophore, siderophore and total microcystins. Community Trajectory Analysis and other multivariate methods were used for analysis resulting in our findings: 1) Microcystis spp. manage copper/iron requirements though a dynamically phased behavior of chalkophore/siderophore production according to their copper and iron limitation status (chalkophore correlates with Cu concentration, R2 = 0.99, and siderophore correlates with the sum of Cu and Fe concentrations, R2 = 0.98). 2) A strong correlation was observed between the production of chalkophore and the cyanobacterial toxin microcystin (R2 = 0.76)-Chalkophore is a predictor of microcystin production. 3) Based on our results and literature, we posit that Microcystis spp. produces microcystin in response to copper/iron availability to manage photosystem productivity and effect an energy-saving status. Results from this work underscore the importance of micronutrients in influencing harmful algal bloom progression and represents a major advance in understanding the ecological function for the cyanobacterial toxin microcystin as a hallmark of micronutrient limitation stress.

Exposure pathways, environmental processes and risks of micro (nano) plastics to crops and feasible control strategies in agricultural regions.

Micro/nanoplastics (MPs/NPs) pollution may adversely impact agricultural ecosystems, threatening the sustainability and security of agricultural production. This drives an urgent need to comprehensively understand the environmental behavior and effects of MPs/NPs in soil and atmosphere in agricultural regions, and to seek relevant pollution prevention strategies. The rhizosphere and phyllosphere are the interfaces where crops are exposed to MPs/NPs. The environmental behavior of MPs/NPs in soil and atmosphere, especially in the rhizosphere and phyllosphere, determines their plant accessibility, bioavailability and ecotoxicity. This article comprehensively reviews the transformation and migration of MPs/NPs in soil, transportation and deposition in the atmosphere, environmental behavior and effects in the rhizosphere and phyllosphere, and plant uptake and transportation pathways. The article also summarizes the key factors controlling MPs/NPs environmental processes, including their properties, biotic and abiotic factors. Based on the sources, environmental processes and intake risks of MPs/NPs in agroecosystems, the article offers several feasible pollution prevention and risk management options. Finally, the review highlights the need for further research on MPs/NPs in agro-systems, including developing quantitative detection methods, exploring transformation and migration patterns in-situ soil, monitoring long-term field experiments, and establishing pollution prevention and control systems. This review can assist in improving our understanding of the biogeochemistry behavior of MPs/NPs in the soil-plant-atmosphere system and provide a roadmap for future research.

Effects of biochar on earthworms during remediation of potentially toxic elements contaminated soils.

With the widespread use of biochar for soil remediation and improvement, its effects on soil organisms are receiving increased attention. The impacts of biochar on earthworms are still poorly understood. This study aimed to assess the potential ecotoxicity of rice husk biochar (RB) and sludge biochar (SB) on earthworms during potentially toxic elements (PTEs) contaminated soil remediation. The results showed that high rates of RB addition (5% and 10%) caused earthworm mortality, but SB addition did not affect earthworm survival. When added at non-lethal rates (3%), RB and SB addition did not affect survival, weight loss, and PTEs accumulation of earthworms, while resulting in apparent avoidance behavior and oxidative stress response. Among them, RB addition was more likely to cause avoidance behavior, while SB addition had a more pronounced stress effect on earthworms. Additionally, the bacterial communities in the earthworm gut were more sensitive to biochar addition than those in soil. SB addition had a greater impact on earthworm gut bacterial communities than RB addition. The addition of RB and SB increased the abundance of Bacillaceae while decreasing the abundance of Rhizobiaceae in the earthworm gut. This change in the composition of bacterial community may impact the nitrogen cycle and organic matter degradation functions of earthworms. The study suggests that RB and SB may have different effects on earthworms during PTEs-contaminated soil remediation, depending on their properties. It will assist us to understand the potential ecotoxicity of biochar and provide several guidance for its safe application.

Metabolomics reveals the molecular mechanism of sewage sludge-derived nutrients and biostimulants stimulating resistance enhancement and the redistribution of carbon and nitrogen metabolism in pakchoi cabbage.

Alkaline thermal hydrolysis of sewage sludge to produce high-quality liquid fertilizer that contains protein, amino acid, organic acid and biostimulants is receiving wide attention, however, the impact on plants and environmental risks must be evaluated for sustainable use. In this study, the interactions between sewage sludge-derived nutrients, biostimulants (SS-NB), and pakchoi cabbage were investigated by a combination of phenotypic and metabolic approaches. Compared with SS-NB0 (single chemical fertilizer), SS-NB100, SS-NB50, and SS-NB25 had no effect on crop yield, but the net photosynthetic rate increased from 1.13 % to 9.82 %. In addition, the antioxidant enzyme activity (SOD) increased from 29.60 % to 71.42 %, with decreasing malondialdehyde (MDA) and H2O2 levels by 84.62-92.93 % and 8.62-18.97 %, respectively, indicating positive effects on photosynthetic and antioxidant capacities. Leaf metabolomics revealed that SS-NB100, SS-NB50, and SS-NB25 resulted in up-regulation of amino acid and alkaloid metabolites, down-regulation of carbohydrate metabolites, and up-regulation and down-regulation of organic acid metabolites, which were involved in carbon and nitrogen redistribution. Galactose metabolism was inactivated by SS-NB100, SS-NB50, and SS-NB25, indicating the protective role of SS-NB in oxidative damage in cells. Furthermore, the application of SS-NB also resulted in a significant reduction in heavy metal contents (Cr, Ni, and Pb) and the target hazard quotient (THQ). The THQ values of Cd, Cr, Ni and Pb were <1.0 in SS-NB50, and may be considered as an optimal fertilization strategy. The result provided better understanding of the phenotypic and metabolic changes imposed by SS-NB-replaced chemical fertilizer nitrogen in pakchoi cabbage leaves.

Pandemic COVID-19 ends but soil pollution increases: Impacts and a new approach for risk assessment.

For three years, a large amount of manufactured pollutants such as plastics, antibiotics and disinfectants has been released into the environment due to COVID-19. The accumulation of these pollutants in the environment has exacerbated the damage to the soil system. However, since the epidemic outbreak, the focus of researchers and public attention has consistently been on human health. It is noteworthy that studies conducted in conjunction with soil pollution and COVID-19 represent only 4 % of all COVID-19 studies. In order to enhance researchers' and the public awareness of the seriousness on the COVID-19 derived soil pollution, we propose the viewpoint that "pandemic COVID-19 ends but soil pollution increases" and recommend a whole-cell biosensor based new method to assess the environmental risk of COVID-19 derived pollutants. This approach is expected to provide a new way for environmental risk assessment of soils affected by contaminants produced from the pandemic.

Plant nanobionics: Fortifying food security via engineered plant productivity.

The world's human population is increasing exponentially, increasing the demand for high-quality food sources. As a result, there is a major global concern over hunger and malnutrition in developing countries with limited food resources. To address this issue, researchers worldwide must focus on developing improved crop varieties with greater productivity to overcome hunger. However, conventional crop breeding methods require extensive periods to develop new varieties with desirable traits. To tackle this challenge, an innovative approach termed plant nanobionics introduces nanomaterials (NMs) into cell organelles to enhance or modify plant function and thus crop productivity and yield. A comprehensive review of nanomaterials affect crop yield is needed to guide nanotechnology research. This article critically reviews nanotechnology applications for engineering plant productivity, seed germination, crop growth, enhancing photosynthesis, and improving crop yield and quality, and discusses nanobionic approaches such as smart drug delivery systems and plant nanobiosensors. Moreover, the review describes NM classification and synthesis and human health-related and plant toxicity hazards. Our findings suggest that nanotechnology application in agricultural production could significantly increase crop yields to alleviate global hunger pressures. However, the environmental risks associated with NMs should be investigated thoroughly before their widespread adoption in agriculture.