Regulation and function of endoplasmic reticulum autophagy in neurodegenerative diseases.

The endoplasmic reticulum, a key cellular organelle, regulates a wide variety of cellular activities. Endoplasmic reticulum autophagy, one of the quality control systems of the endoplasmic reticulum, plays a pivotal role in maintaining endoplasmic reticulum homeostasis by controlling endoplasmic reticulum turnover, remodeling, and proteostasis. In this review, we briefly describe the endoplasmic reticulum quality control system, and subsequently focus on the role of endoplasmic reticulum autophagy, emphasizing the spatial and temporal mechanisms underlying the regulation of endoplasmic reticulum autophagy according to cellular requirements. We also summarize the evidence relating to how defective or abnormal endoplasmic reticulum autophagy contributes to the pathogenesis of neurodegenerative diseases. In summary, this review highlights the mechanisms associated with the regulation of endoplasmic reticulum autophagy and how they influence the pathophysiology of degenerative nerve disorders. This review would help researchers to understand the roles and regulatory mechanisms of endoplasmic reticulum-phagy in neurodegenerative disorders.

GPR50 regulates neuronal development as a mitophagy receptor.

Neurons rely heavily on high mitochondrial metabolism to provide sufficient energy for proper development. However, it remains unclear how neurons maintain high oxidative phosphorylation (OXPHOS) during development. Mitophagy plays a pivotal role in maintaining mitochondrial quality and quantity. We herein describe that G protein-coupled receptor 50 (GPR50) is a novel mitophagy receptor, which harbors the LC3-interacting region (LIR) and is required in mitophagy under stress conditions. Although it does not localize in mitochondria under normal culturing conditions, GPR50 is recruited to the depolarized mitochondrial membrane upon mitophagy stress, which marks the mitochondrial portion and recruits the assembling autophagosomes, eventually facilitating the mitochondrial fragments to be engulfed by the autophagosomes. Mutations Δ502-505 and T532A attenuate GPR50-mediated mitophagy by disrupting the binding of GPR50 to LC3 and the mitochondrial recruitment of GPR50. Deficiency of GPR50 causes the accumulation of damaged mitochondria and disrupts OXPHOS, resulting in insufficient ATP production and excessive ROS generation, eventually impairing neuronal development. GPR50-deficient mice exhibit impaired social recognition, which is rescued by prenatal treatment with mitoQ, a mitochondrially antioxidant. The present study identifies GPR50 as a novel mitophagy receptor that is required to maintain mitochondrial OXPHOS in developing neurons.

Temperature-dependent jumonji demethylase modulates flowering time by targeting H3K36me2/3 in Brassica rapa.

Global warming has a severe impact on the flowering time and yield of crops. Histone modifications have been well-documented for their roles in enabling plant plasticity in ambient temperature. However, the factor modulating histone modifications and their involvement in habitat adaptation have remained elusive. In this study, through genome-wide pattern analysis and quantitative-trait-locus (QTL) mapping, we reveal that BrJMJ18 is a candidate gene for a QTL regulating thermotolerance in thermotolerant B. rapa subsp. chinensis var. parachinensis (or Caixin, abbreviated to Par). BrJMJ18 encodes an H3K36me2/3 Jumonji demethylase that remodels H3K36 methylation across the genome. We demonstrate that the BrJMJ18 allele from Par (BrJMJ18Par) influences flowering time and plant growth in a temperature-dependent manner via characterizing overexpression and CRISPR/Cas9 mutant plants. We further show that overexpression of BrJMJ18Par can modulate the expression of BrFLC3, one of the five BrFLC orthologs. Furthermore, ChIP-seq and transcriptome data reveal that BrJMJ18Par can regulate chlorophyll biosynthesis under high temperatures. We also demonstrate that three amino acid mutations may account for function differences in BrJMJ18 between subspecies. Based on these findings, we propose a working model in which an H3K36me2/3 demethylase, while not affecting agronomic traits under normal conditions, can enhance resilience under heat stress in Brassica rapa.

Rhizospheric microbiota of suppressive soil protect plants against Fusarium solani infection.

BACKGROUND: Fusarium infection has caused huge economic losses in many crops. The study aimed to compare the microbial community of suppressive and conducive soils and relate to the reduction of Fusarium wilt. RESULTS: High-throughput sequencing and microbial network analysis were used to investigate the differences in the rhizosphere microbiota of the suppressive and conducive soils and to identify the beneficial keystone taxa. Plant pathogens were enriched in the conducive soil. Potential plant-beneficial microorganisms and antagonistic microorganisms were enriched in the suppressive soil. More positive interactions and keystone taxa existed in the suppressive soil network. Thirty-nine and 16 keystone taxa were identified in the suppressive and conducive soil networks, respectively. Sixteen fungal strains and 168 bacterial strains were isolated from suppressive soil, some of which exhibited plant growth-promotion traits. Thirty-nine bacterial strains and 10 fungal strains showed antagonistic activity against F. solani. Keystone taxa Bacillus and Trichoderma exhibited high antifungal activity. Lipopeptides produced by Bacillus sp. RB150 and chitinase from Trichoderma spp. inhibited the growth of F. solani. Microbial consortium I (Bacillus sp. RB150, Pseudomonas sp. RB70 and Trichoderma asperellum RF10) and II (Bacillus sp. RB196, Bacillus sp. RB150 and T. asperellum RF10) effectively controlled root rot disease, the spore number of F. solani was reduced by 94.2% and 83.3%. CONCLUSION: Rhizospheric microbiota of suppressive soil protects plants against F. solani infection. Antagonistic microorganisms in suppressive soil inhibit pathogen growth and infection. Microbial consortia consisted of keystone taxa well control root rot disease. These findings help control Fusarium wilt. © 2024 Society of Chemical Industry.

Constructing highly efficient bifunctional catalysts for oxygen reduction and oxygen evolution by modifying MXene with transition metal.

Exploring highly active electrocatalysts for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) has become a growing interest in recent years. Herein, an efficient pathway for designing MXene-based ORR/OER catalysts is proposed. It involves introducing non-noble metals into Vo (vacancy site), H1 and H2 (the hollow sites on top of C and the metal atom, respectively) sites on M2CO2 surfaces, named TM-VO/H1/H2-M2CO2 (TM = Fe, Co, Ni, M = V, Nb, Ta). Among these recombination catalysts, Co-H1-V2CO2 and Ni-H1-V2CO2 exhibit the most promising ORR catalytic activities, with low overpotential values of 0.35 and 0.37 V, respectively. Similarly, Fe-H1-V2CO2, Co-VO-Nb2CO2, and Ni-H2-Nb2CO2 possess low OER overpotential values of 0.29, 0.39, and 0.44 V, respectively, suggesting they have enormous potential as effective catalysts for OER. Notably, Co-H2-Ta2CO2 possesses the lowest potential gap value of 0.53 V, demonstrating it has an extraordinary bifunctional catalytic activity. The excellent catalytic performance of these recombination catalysts can be elucidated through an electronic structure analysis, which primarily relies on the electron-donating capacity and synergistic effects between transition metals and sub-metals. These results provide theoretical guidance for designing new ORR and OER catalysts using 2D MXene materials.

Theoretical prediction of emerging high-performance trifunctional ORR/OER/HER single-atom catalysts: transition metals anchored into π-π conjugated graphitic carbon nitride (g-C10N3).

The design of high-performance trifunctional oxygen reduction/oxygen evolution/hydrogen evolution reactions (ORR/OER/HER) electrocatalysts has become the current research focus. In this work, we report a series of single-atom catalysts formed by nine kinds of transition metal (Fe, Co, Ni, Ru, Rh, Pd, Os, Ir, and Pt) anchored in g-C10N3 (namely TM@g-C10N3) as promising trifunctional electrocatalysts to replace precious metal catalysts by density functional theory methods. All TM@g-C10N3 have good thermodynamic and electrochemical stability. Especially, Rh@g-C10N3 and Ir@g-C10N3 exhibit extremely low ORR/OER overpotentials with the values of 0.26/0.28 V and 0.34/0.41 V, respectively. Besides, their hydrogen adsorption free energy values are close to Pt(111), with their values being 0.16 and -0.16 eV, respectively. The calculated results indicate that Rh@g-C10N3 and Ir@g-C10N3 can become trifunctional electrocatalysts with great probability. Through the analysis of the dynamic mechanism for Rh@g-C10N3, it can be concluded that the four-electron ORR pathway is more conducive to occurring on Rh@g-C10N3 because the energy barrier forming this pathway is lower. Besides, the step of *OH + H+ + e- → * + H2O has the highest energy barrier in dynamics, which is consistent with this step being a potential determining step in thermodynamics. Ultimately, the solvation effect considered has little effect on the catalytic performance of screened Rh@g-C10N3 and Ir@g-C10N3, and even at a high temperature of 500 K, the structures of these two catalysts have no significant distortion after 2 ps simulations. Our calculations will provide clear guidance for future experimental synthesis and design of such catalysts.

Rare Earth Metal Anchored into Nitrogen-Doped Graphene for CO2 Electrocatalytic Reduction to C1 Products.

Single-atom catalysts (SACs) are attracting global attention due to their 100% atomic utilization rate and unique properties. Rare-earth-based SACs have shown great potential in the field of electrocatalysis in recent years. In this study, the catalytic performance of four rare earth metals (REMs) anchored into N-graphene for the CO2RR is systematically studied by density functional theory. The calculation results of formation energy show that all REM@N6-G compounds have favorable stability. In addition, the Gibbs free energy calculation results of all possible elementary reactions show that the *OCHO pathway is the optimal hydrogenation pathway for all catalysts, and they have the same potential determining step (*OCHO + e- + H+ → *HCOOH). Meanwhile, the products of the CO2RR on these catalysts are different, and the product on REM@N6-G (REM = La, Pr, and Nd) is CH4, while the product on Ce@N6-G is CH3OH. In particular, Nd@N6-G exhibits the best catalytic activity in this work, with a very low limiting potential of -0.38 V. These results may guide the development of rare-earth-based SACs for CO2RR.

BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa.

Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa.

Transcription Factor Spo0A Regulates the Biosynthesis of Difficidin in Bacillus amyloliquefaciens.

Bacillus amyloliquefaciens WH1 produces multiple antibiotics with antimicrobial activity and can control bacterial wilt disease caused by Ralstonia solanacearum. Antibacterial substances produced by WH1 and the regulation mechanism are unknown. In this study, it was found that difficidin, and to a minor extent bacillibactin, exhibited antibacterial activity against R. solanacearum. Lipopeptides, macrolactin, bacillaene, and bacilysin had no antibacterial activity. Ferric iron uptake transcriptional regulator Fur bound the promoter region of the dhb gene cluster of bacillibactin biosynthesis. Mutant Δfur showed a higher bacillibactin production and its antibacterial activity increased by 27% than wild-type WH1. Difficidin inhibited R. solanacearum growth and disrupted the integrity of the cells. Lack of transcription factor Spo0A abolished difficidin biosynthesis. Spo0A bound the promoter region of the dfn gene cluster of difficidin biosynthesis. Changing phosphorylation levels of Spo0A via deletion of phosphatase gene spo0E and histidine kinases genes kinA and kinD affected the biosynthesis of difficidin. Deletion of spo0E increased the phosphorylation level of Spo0A and consequently improved the difficidin production. The antibacterial activity of mutant Δspo0E and ΔkinA increased by 12% and 19%. The antibacterial activity of mutant ΔkinD decreased by 28%. Collectively, WH1 produced difficidin to disrupt the cell of R. solanacearum and secreted siderophore bacillibactin to compete for ferric iron. Spo0A regulated difficidin biosynthesis. Spo0A regulates quorum-sensing responses and controls the biosynthesis of secondary metabolites in B. amyloliquefaciens. This study has important findings in the regulation mechanism of antibiotic synthesis and helps to improve antibiotic yield in Bacillus. IMPORTANCE Pathogen R. solanacearum causes bacterial wilt disease in many crops. There is no chemical bactericide that can control bacterial wilt disease. It is vital to find antagonistic microorganisms and antibacterial substances that can efficiently control bacterial wilt disease. B. amyloliquefaciens WH1 could inhibit the growth of R. solanacearum. Via genetic mutation, it was found that difficidin and to a minor extent bacillibactin produced by WH1 acted efficiently against R. solanacearum. The transcription factor Spo0A regulated the synthesis of difficidin. Phosphorylation of Spo0A affected the production of difficidin. Increasing the phosphorylation level of Spo0A improved the difficidin production and antibacterial activity. In-depth analysis of the regulation mechanism of antibiotic difficidin is meaningful for enhancing the control efficiency of WH1. B. amyloliquefaciens WH1 and the antibacterial substances have vast application potential in controlling bacterial wilt disease.

Oxo transition metal anchored on C3N4 with constructing a high-activity bifunctional electrocatalyst for rechargeable metal-air batteries.

It is urgent to find efficient bifunctional electrocatalysts of oxygen reduction and evolution reactions to catalyze the oxygen electrode reaction of metal-air batteries. Herein, oxo transition metal anchored on C3N4 as bifunctional oxygen electrocatalyst is investigated using density functional theory calculations. Various stability analysis results show that all catalysts in this study have excellent stability. In particular, for the two sites of the catalyst, the calculated results show that the effect of the ß site on the reaction species is generally stronger than that of the α site, while the catalytic activity of the α site is slightly better than that of ß site. In particular, the α site on Ni2@C3N4 has the lowest overpotential (ηORR = 0.44 V, ηOER = 0.51 V) and bifunctional index value (BI = 0.95 V). Finally, the linear relationships between eight activity descriptors and the adsorption strength of reaction intermediates are used to analyze the influencing factors of the effective activity of the catalyst. The results reflect that the activity descriptors can well describe the change in adsorption strength of intermediates on the catalyst. Thus, this work provides a good idea for designing excellent bifunctional catalysts for rechargeable metal-air batteries.

Wall-associated kinase BrWAK1 confers resistance to downy mildew in Brassica rapa.

The plant cell wall is the first line of defence against physical damage and pathogen attack. Wall-associated kinase (WAK) has the ability to perceive the changes in the cell wall matrix and transform signals into the cytoplasm, being involved in plant development and the defence response. Downy mildew, caused by Hyaloperonospora brassicae, can result in a massive loss in Chinese cabbage (Brassica rapa L. ssp. pekinensis) production. Herein, we identified a candidate resistant WAK gene, BrWAK1, in a major resistant quantitative trait locus, using a double haploid population derived from resistant inbred line T12-19 and the susceptible line 91-112. The expression of BrWAK1 could be induced by salicylic acid and pathogen inoculation. Expression of BrWAK1 in 91-112 could significantly enhance resistance to the pathogen, while truncating BrWAK1 in T12-19 increased disease susceptibility. Variation in the extracellular galacturonan binding (GUB) domain of BrWAK1 was found to mainly confer resistance to downy mildew in T12-19. Moreover, BrWAK1 was proved to interact with BrBAK1 (brassinosteroid insensitive 1 associated kinase), resulting in the activation of the downstream mitogen-activated protein kinase (MAPK) cascade to trigger the defence response. BrWAK1 is the first identified and thoroughly characterized WAK gene conferring disease resistance in Chinese cabbage, and the plant biomass is not significantly influenced by BrWAK1, which will greatly accelerate Chinese cabbage breeding for downy mildew resistance.

Single transition metal-decorated C4N/MoS2 heterostructure for boosting oxygen reduction, oxygen evolution, and hydrogen evolution.

Multifunctional electrocatalysts for oxygen reduction reaction (ORR), oxygen evolution reaction (OER), and hydrogen evolution (HER) are required preconditions for the development of a highly promising new green energy conversion and storage technology. Herein, a comprehensive computation of the ORR, OER and HER catalytic performance for the pristine and metal-decorated C4N/MoS2 (TM-C4N/MoS2) is researched using density functional theory. Remarkably, Pd-C4N/MoS2 exhibits distinguished bifunctional catalytic performance with lower ORR/OER overpotentials of 0.34/0.40 V. Rh-C4N/MoS2 is the prospective trifunctional catalyst with the low ORR/OER/HER overpotentials of 0.48/0.55/-0.16 V, but its electrochemical stability needs to be further improved. Furthermore, the strong correlation between intrinsic descriptor (φ) and adsorption free energy of *OH verifies that the catalytic activity of TM-C4N/MoS2 is affected by active metal and surrounding coordination environment. The heap map has summarized the correlations of d-band center, adsorption free energy of reaction species, and φ as the vital parameter for ORR/OER overpotentials of designing catalysts. The electronic structure analysis uncovers the activity enhancement is due to the adjustable adsorption behavior of reaction intermediates on TM-C4N/MoS2. This finding paves the way to develop high-activity and multifunctional catalysts, making them suitable for multifunctional applications in the forthcoming critically needed green energy conversion and storage technologies.

The cellular redox state in Bacillus amyloliquefaciens WH1 affects biofilm formation indirectly in a surfactant direct manner.

Surfactin is a signal to trigger biofilm formation against harsh environments. Generally, harsh environments can result in change of the cellular redox state to induce biofilm formation, but we know little about whether the cellular redox state influences biofilm formation via surfactin. Here, the reductant glucose could reduce surfactin and enhance biofilm formation by a surfactin-indirect way. The oxidant H2 O2 led to a decrease of surfactin accompanying with weakened biofilm formation. Spx and PerR were both necessary for surfactin production and biofilm formation. H2 O2 improved surfactin production but inhibited biofilm formation by a surfactin-indirect manner in Δspx, while it reduced surfactin production without obvious influence on biofilm formation in ΔperR. The ability against H2 O2 stress was enhanced in Δspx, but weakened in ΔperR. Thereby, PerR was favorable for resisting oxidative stress, while Spx played a negative role in this action. Knockout and compensation of rex also supported that the cells could form biofilm by a surfactin-indirect way. Collectively, surfactin is not a unique signal to trigger biofilm formation, and the cellular redox state can influence biofilm formation by a surfactin-direct or -indirect way in Bacillus amyloliquefaciens WH1.

Theoretical Screening of Highly Efficient Single-Atom Catalysts Based on Covalent Triazine Frameworks for Oxygen Reduction.

Covalent triazine frameworks (CTFs) obtained from the trimerization of aromatic nitriles are expected to be the preferred carrier for single-atom catalysts (SACs). Using density functional theory methods, the oxygen reduction reaction (ORR) performance of a series of 3d, 4d, and 5d transition metals supported on the 6N or 9N pore of the CTF system [M-CTF(6N) or M-CTF(9N)] is explored. At first, 32 kinds of M-CTF(6N) and M-CTF(9N) are screened out with high thermodynamic and electrochemical stability. The binding energy of ORR intermediates and the change of Gibbs free energy in each step of the ORR are calculated. The overpotential of Pd-CTF(6N) is the lowest, which is 0.38 V. Considering that the ORR activity of M-CTFs is mainly limited by the strong binding of *OH, M-CTF(6N) and M-CTF(9N) are further modified by the OH ligand, namely, M-OH-CTF(6N) and M-OH-CTF(9N). After being modified by the OH ligand, due to the weakened binding strength of *OH, all these screened M-CTFs exhibit better ORR activity. Among them, the η values of Cu-OH-CTF(6N), Pd-OH-CTF(6N), Rh-OH-CTF(6N), Ir-OH-CTF(6N), Rh-OH-CTF(9N), and Ir-OH-CTF(9N) are 0.39, 0.38, 0.24, 0.30, 0.31, and 0.33 V, respectively, which possess better ORR activity than the Pt(111) surface (η = 0.45 V). This work highlights the great potential of CTFs as an efficient carrier for SACs.

Application of nematicide avermectin enriched antibiotic-resistant bacteria and antibiotic resistance genes in farmland soil.

The extensive use of antibiotics in medicine and agriculture has resulted in the accumulation of antibiotic-resistant microorganisms and antibiotic resistance genes (ARGs) in environments, which threaten human health and contaminate environment. Nematicide avermectin is widely applied to control root-knot nematodes. The effect of five-years application of avermectin on rhizosphere microbiome and resistome of sick tobacco plants in farmland were investigated in present study. The environmental risks of avermectin was assessed adequately. Metagenomic method was used to analyze antibiotic-resistant bacteria and antibiotic resistance genes in the avermectin-treated soil. The abundance and distribution of antibiotic-resistant bacteria and their antibiotic resistance genes were affected by avermectin application. The antibiotic resistant Proteobacteria occupied the highest percentage (36%) in rhizosphere soil and carried 530 ARGs. Opportunistic human pathogens carrying antibiotic resistance genes were enriched in the avermectin-treated soil. Avermectin application increased the counts of many types of antibiotic resistance genes. The relative abundances of genes adeF, BahA, fusH, ileS, and tlrB in the avermectin-treated soil were significantly greater than in the untreated control soil. Different resistance mechanisms were revealed in the avermectin-treated soil. The efflux of antibiotic (670 ARGs), inactivation of antibiotic (475 ARGs), and alteration of antibiotic target (267 ARGs) were the main resistance mechanisms. Rigid control the avermectin dose and use frequency and other pesticides can decrease soil antibiotic resistance genes and protect agricultural products' safety and public health. Overall, application of nematicide avermectin enriched antibiotic-resistant bacteria and antibiotic resistance genes in farmland soil, which should be on the alert for environment protection.

Recent Advancements and Biotechnological Implications of Carotenoid Metabolism of Brassica.

Carotenoids were synthesized in the plant cells involved in photosynthesis and photo-protection. In humans, carotenoids are essential as dietary antioxidants and vitamin A precursors. Brassica crops are the major sources of nutritionally important dietary carotenoids. Recent studies have unraveled the major genetic components in the carotenoid metabolic pathway in Brassica, including the identification of key factors that directly participate or regulate carotenoid biosynthesis. However, recent genetic advances and the complexity of the mechanism and regulation of Brassica carotenoid accumulation have not been reviewed. Herein, we reviewed the recent progress regarding Brassica carotenoids from the perspective of forward genetics, discussed biotechnological implications and provided new perspectives on how to transfer the knowledge of carotenoid research in Brassica to the crop breeding process.

Cooperative Action of Fulvic Acid and Bacillus paralicheniformis Ferment in Regulating Soil Microbiota and Improving Soil Fertility and Plant Resistance to Bacterial Wilt Disease.

Excessive continuous cropping and soil degradation, such as acidification, hardening, fertility decline, and the degradation of microbial community, lead to the epidemic of soilborne diseases and cause great loss in agriculture production. Application of fulvic acid can improve the growth and yield of various crops and effectively suppress soilborne plant diseases. Bacillus paralicheniformis strain 285-3 producing poly-gamma-glutamic acid is used to remove the organic acid that can cause soil acidification and increase the fertilizer effect of fulvic acid and the effect of improving soil quality and inhibiting soilborne disease. In field experiments, the application of fulvic acid and Bacillus paralicheniformis ferment effectively reduced the incidence of bacterial wilt disease and improved soil fertility. Both fulvic acid powder and B. paralicheniformis ferment improved soil microbial diversity and increased the complexity and stability of the microbial network. For B. paralicheniformis ferment, the molecular weight of poly-gamma-glutamic acid became smaller after heating, which could better improve the soil microbial community and network structure. In fulvic acid and B. paralicheniformis ferment-treated soils, the synergistic interaction between microorganisms increased and the number of keystone microorganisms increased, which included antagonistic bacteria and plant growth-promoting bacteria. Changes in the microbial community and network structure were the main reason for the reduced incidence of bacterial wilt disease. Application of fulvic acid and Bacillus paralicheniformis ferment improved soil physicochemical properties and effectively controlled bacterial wilt disease by changing microbial community and network structure and enriching antagonistic and beneficial bacteria. IMPORTANCE Continuous cropping tobacco has led to soil degradation and caused soilborne bacterial wilt disease. Fulvic acid as a biostimulator was applied to restore soil and control bacterial wilt disease. For improving its effect, fulvic acid was fermented with Bacillus paralicheniformis strain 285-3 producing poly-gamma-glutamic acid. Fulvic acid and B. paralicheniformis ferment inhibited bacterial wilt disease, improved soil quality, enriched beneficial bacteria, and increased microbial diversity and microbial network complexity. Some keystone microorganisms in fulvic acid and B. paralicheniformis ferment-treated soils had potential antimicrobial activity and plant growth-promoting attributes. Fulvic acid and B. paralicheniformis 285-3 ferment could be used to restore soil quality and microbiota and control bacterial wilt disease. This study found new biomaterial to control soilborne bacterial disease by combining fulvic acid and poly-gamma-glutamic acid application.

Microbial network and composition changes according to tobacco varieties and interferes differently in black shank disease defense.

AIMS: The soil-borne oomycete pathogen Phytophthora parasitica can cause black shank disease in tobacco plants. The use of resistant varieties can be used to control black shank disease. The potential relationships of the composition of the rhizosphere microbiome to resistance to black shank disease are poorly understood. This work aims to compare the rhizosphere microbial community and network of the tobacco resistant variety HB202 with the susceptible variety XY3. METHODS AND RESULTS: Rhizospheric soils were collected from tobacco plants of HB202 and XY3 in the fields with same soil types and agricultural operations. The compositions of the rhizosphere microbial communities were revealed by Illumina sequencing of bacterial 16S rRNA genes and fungal spacer (ITS) sequences and analysed with molecular ecological network pipeline. The alpha diversity of fungal communities of the two varieties was significantly different. The structure and composition of bacterial and fungal communities in the resistant variety in the rhizosphere was different from the susceptible variety. Relative abundances of beneficial genera in the HB202 microbiota were higher than in the XY3. Conversely, the XY3 microbiota exhibited a higher abundance of deleterious genera compared to the HB202 microbiota. The resistant variety influences the topological properties and microbial interactions in the rhizosphere against the disease. The network of the HB202 was more complex and had higher connectivity compared to the XY3 network. CONCLUSIONS: The rhizosphere microbial communities and networks of two tobacco varieties are very different. These changes in the microbial communities and their interactions may play an important role in tobacco resistance to black shank disease.

The Endophytic Root Microbiome Is Different in Healthy and Ralstonia solanacearum-Infected Plants and Is Regulated by a Consortium Containing Beneficial Endophytic Bacteria.

Plant bacterial wilt disease caused by Ralstonia solanacearum leads to huge economic losses worldwide. Endophytes play vital roles in promoting plant growth and health. It is hypothesized that the endophytic root microbiome and network structure are different in healthy and diseased plants. Here, the endophytic root microbiomes and network structures of healthy and diseased tobacco plants were investigated. Composition and network structures of endophytic root microbiomes were distinct between healthy and diseased plants. Healthy plants were enriched with more beneficial bacteria and bacteria with antagonistic activity against R. solanacearum. R. solanacearum was most abundant in diseased plants. Microbial networks in diseased plants had fewer modules and edges, lower connectivity, and fewer keystone microorganisms than those in healthy plants. Almost half of the nodes were unique in the two networks. Ralstonia was identified as a key microorganism of the diseased-plant network. In healthy plants, abundant bacteria and biomarkers (Pseudomonas and Streptomyces) and keystone microorganisms (Bacillus, Lysobacter, and Paenibacillus) were plant-beneficial bacteria and showed antibacterial and plant growth-promoting activities. The endophytic strain Bacillus velezensis E9 produced bacillaene to inhibit R. solanacearum. Consortia containing keystone microorganisms and beneficial endophytic bacteria significantly regulated the endophytic microbiome and attenuated bacterial wilt by inducing systemic resistance and producing antibiotic. Overall, the endophytic root microbiome and network structure in diseased plants were different from those in healthy plants. The endophytic root microbiome of diseased plants had low abundances of beneficial bacteria and an unstable network and lacked beneficial keystone microorganisms, which favored infection. Synthetic microbial consortia were effective measures for preventing R. solanacearum infection. IMPORTANCE Bacterial wilt disease causes heavy yield losses in many crops. Endophytic microbiomes play important roles in control of plant diseases. However, the role of the endophytic root microbiome in controlling bacterial wilt disease is poorly understood. Here, differences in endophytic root microbiomes and network structures between healthy and diseased tobacco plants are reported. A synthetic microbial consortium containing beneficial endophytic bacteria was used to regulate the endophytic microbiome and attenuate bacterial wilt disease. The results could be generally used to guide control of bacterial wilt disease.

Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network.

Alzheimer's disease (AD) is a neurodegenerative disorder with cognitive impairment and abnormal mental behaviour. There is currently no effective cure. The development of early diagnostic markers and the mining of potential therapeutic targets are one of the important strategies. This study aimed to explore potential biomarkers or therapeutic targets related to AD in the hippocampus and prefrontal cortex, two brain regions highly related to AD. Differentially expressed genes and miRNAs between AD patients and healthy controls were obtained from the Gene Expression Omnibus database. The mRNA-miRNA network was constructed and key genes involved in AD were screened out by protein-protein interaction analysis, and were subsequently verified by independent datasets and qPCR in an AD mouse model. Our findings showed that six hub genes including CALN1, TRPM7, ATR, SOCS3, MOB3A and OGDH were believed to be involved in the pathogenesis of AD. Western blot analysis further determined that CALN1, ATR and OGDH were the possible biomarkers and therapeutic targets for AD. In addition, 6 possible miRNAs biomarkers have also been verified by qPCR on AD animal models. Our findings may benefit clinical diagnosis and early prevention of AD.