ABSTRACT
OBJECTIVE: The efficacy of single-incision plus one-port laparoscopic surgery (SILS + 1) versus conventional laparoscopic surgery (CLS) for colorectal cancer treatment remains unclear. This study compares the short-term and long-term outcomes of SILS + 1 and CLS using a high-quality systematic review and meta-analysis. METHOD: Literature search followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, drawing from PubMed, Embase, Web of Science, and the Cochrane Library until December 10, 2023. Statistical analysis was conducted using RevMan and Stata. RESULT: The review and meta-analysis included seven studies with 1740 colorectal cancer patients. Compared to CLS, SILS + 1 showed significant improvements in operation time (WMD = - 18.33, P < 0.00001), blood loss (WMD = - 21.31, P < 0.00001), incision length (WMD = - 2.07, P < 0.00001), time to first defecation (WMD = - 14.91, P = 0.009), time to oral intake (WMD = - 11.46, P = 0.04), and time to ambulation (WMD = - 11.52, P = 0.01). There were no significant differences in lymph node harvest, resection margins, complications, anastomotic leakage, hospital stay, disease-free survival, overall survival, and postoperative recurrence. CONCLUSIONS: Compared to CLS, SILS + 1 demonstrates superiority in shortening the surgical incision and promoting postoperative recovery. SILS + 1 can provide a safe and feasible alternative to CLS.
Subject(s)
Colorectal Neoplasms , Laparoscopy , Humans , Colorectal Neoplasms/surgery , Treatment Outcome , Operative Time , Postoperative Complications/etiology , Length of Stay , Female , Male , Neoplasm Recurrence, Local , Middle AgedABSTRACT
Dysregulation of long noncoding RNAs (lncRNAs) is involved in the development of colorectal cancer (CRC). In the present study, the identification of muscle blind like splicing regulator 1 antisense RNA 1 (MBNL1AS1) lncRNA was reported. Firstly, Cell Counting Kit8, EdU and colony formation assays were uesed to explore the role of MBNL1AS1 in regulating the proliferation of CRC cells. According to TCGA database, it was found that MBNL1AS1 was correlated with microRNA (miR)29c3p and blood vessel epicardial substance (BVES) expression in CRC cells. Then, the regulation among MBNL1AS1, miR29C3P and BVES was detected by dual luciferase reporter assay and the function of MBNL1AS1/miR29C3P/BVES axis was explored by rescue assay. The results demonstrated that MBNL1AS1 expression was decreased in CRC and was associated with the size of tumors derived from patients with CRC. Functionally, the upregulation of MBNL1AS1 suppressed CRC cell proliferation in vitro and inhibited tumor growth in vivo, while knockdown of MBNL1AS1 expression caused the opposite effects. MBNL1AS1 expression correlated with BVES expression in CRC tissues and MBNL1AS1 enhanced the stability of BVES mRNA by functioning as a competing endogenous RNA to sponge miR29c3p; the latter directly targeted MBNL1AS1 and BVES mRNA 3'UTR. Collectively, the results indicated that MBNL1AS1 suppressed CRC cell proliferation by regulating miR29c3p/BVES signaling, suggesting that the MBNL1AS1/miR29c3p/BVES axis may be a potential therapeutic target for CRC.
Subject(s)
Colorectal Neoplasms , MicroRNAs , Humans , RNA, Antisense , Muscles , Cell Proliferation/genetics , Colorectal Neoplasms/genetics , MicroRNAs/genetics , RNA-Binding Proteins/genetics , Muscle Proteins , Cell Adhesion MoleculesABSTRACT
Background: For cancer patients, red blood cell distribution width (RDW) is a readily accessible and cost-effective preoperative prognostic predictor. This study aimed to determine whether RDW is a predictive factor for individuals undergoing radical surgery for gastric cancer (GC). Methods: A literature search was performed to select relevant studies for inclusion in the subsequent meta-analysis. Relevant data were pooled to assess the association between RDW and GC results, including overall survival (OS), disease-free survival (DFS), and cancer-specific survival (CSS), as well as clinicopathological features. Results: The meta-analysis and systemic review included data from 8 studies comprising 1,587 individuals diagnosed with GC. In this context, RDW refers to the coefficient of variation of RDW (RDW-CV). A high level of RDW-CV was significantly associated with older age [odds ratio (OR) =2.25; 95% confidence interval (CI): 1.72-2.94; P<0.00001], larger tumor diameter (OR =1.90; 95% CI: 1.42-2.56; P<0.0001), and vascular invasion (OR =2.22; 95% CI: 1.10-4.49; P=0.03). After hazard ratios (HRs) and 95% CIs were pooled, RDW-CV was found to be an independent prognostic factor of OS (HR =1.79; 95% CI: 1.21-2.66; I2=85%; P=0.004), DFS (HR =1.81; 95% CI: 1.37-2.39; I2=0%; P<0.0001), and CSS (HR =2.73; 95% CI: 1.36-5.49; I2=0%; P=0.005) in patients with GC. Conclusions: The association between high levels of RDW-CV and poor survival in GC suggests that RDW-CV may be a viable prognostic indicator for patients with GC.